This document describes a study that used a photometric assay to measure the antioxidant power of various beverage samples like orange juice, apple juice, and different teas using a Thermo Scientific Multiskan FC microplate photometer. The assay measured the ability of samples to reduce copper ions and form a complex that is detected photometrically. Green tea showed the highest antioxidant power while orange and apple juices showed the lowest. The microplate reader provided high sensitivity and precision for the assay measurements.
Evaluation of antioxidant and antiradical properties of pomegranate (punica g...Pritam Kolge
Evaluation of antioxidant and antiradical properties
of Pomegranate (Punica granatum L.) seed and defatted
seed extracts
This is Journal Club activity Presentation with the reference of various research papers.
This Presentation Contain following...
#Info about Paper
#Abstract
#Materials
#Methods
#Results
#Discussion
#Conclusion
#References
*Important Methods used
#Moisture content
#Fat content
#Acid value
#Peroxide value
#Oxidative stability index
#Total phenols content
#Preparation of Pomegranate seed extracts and calculate extract yield
#Evaluation of antioxidant properties of Pomegranate seed extracts using
-DPPH radicals scavenging activity
-FRAP assay
#Antioxidant efficiency of seed extract (Oxidative stability extract)
#Statistical analysis
Journal Club Presentation at Bharati Vidyapeeth College of Pharmacy, Kolhapur.
Thanks for Help and Guidance of Dr. P. B. Choudhari (Assistant Professor, Pharmaceutical Chemistry) and Dr. A. J. Shinde (Assistant Professor, Department of Pharmaceutics)
Evaluation of antioxidant properties of pomegranate peel extract in compariso...Pritam Kolge
Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract.....
This is Journal Club activity Presentation with the reference of various research papers.
This Presentation Contain following...
#Info about Paper
#Highlights
#Introduction
#Materials
#Methods
#Results
#Discussion
#Conclusion
#References
Important Methods used
1)Antioxidants extraction
2)Determination of total phenolics content
3)FRAP Assay
4)Determination of content of phenolic compounds in the
extract
5)Superoxide radical scavenging activity (DPPH)
6)Hydroxyl radical (OH) prevention activity
Journal Club Presentation at Bharati Vidyapeeth College of Pharmacy, Kolhapur.
Thanks for Help and Guidance of Dr. P. B. Choudhari (Assistant Professor, Pharmaceutical Chemistry) and Mr. D. P. Mali Assistant Professor, Pharmaceutical Chemistry
This slide is about grape seed extract and their structures, along with the extraction methods of grape seed oils. explains about the uses and side effects.Their antioxidant property.
Evaluation of antioxidant and antiradical properties of pomegranate (punica g...Pritam Kolge
Evaluation of antioxidant and antiradical properties
of Pomegranate (Punica granatum L.) seed and defatted
seed extracts
This is Journal Club activity Presentation with the reference of various research papers.
This Presentation Contain following...
#Info about Paper
#Abstract
#Materials
#Methods
#Results
#Discussion
#Conclusion
#References
*Important Methods used
#Moisture content
#Fat content
#Acid value
#Peroxide value
#Oxidative stability index
#Total phenols content
#Preparation of Pomegranate seed extracts and calculate extract yield
#Evaluation of antioxidant properties of Pomegranate seed extracts using
-DPPH radicals scavenging activity
-FRAP assay
#Antioxidant efficiency of seed extract (Oxidative stability extract)
#Statistical analysis
Journal Club Presentation at Bharati Vidyapeeth College of Pharmacy, Kolhapur.
Thanks for Help and Guidance of Dr. P. B. Choudhari (Assistant Professor, Pharmaceutical Chemistry) and Dr. A. J. Shinde (Assistant Professor, Department of Pharmaceutics)
Evaluation of antioxidant properties of pomegranate peel extract in compariso...Pritam Kolge
Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract.....
This is Journal Club activity Presentation with the reference of various research papers.
This Presentation Contain following...
#Info about Paper
#Highlights
#Introduction
#Materials
#Methods
#Results
#Discussion
#Conclusion
#References
Important Methods used
1)Antioxidants extraction
2)Determination of total phenolics content
3)FRAP Assay
4)Determination of content of phenolic compounds in the
extract
5)Superoxide radical scavenging activity (DPPH)
6)Hydroxyl radical (OH) prevention activity
Journal Club Presentation at Bharati Vidyapeeth College of Pharmacy, Kolhapur.
Thanks for Help and Guidance of Dr. P. B. Choudhari (Assistant Professor, Pharmaceutical Chemistry) and Mr. D. P. Mali Assistant Professor, Pharmaceutical Chemistry
This slide is about grape seed extract and their structures, along with the extraction methods of grape seed oils. explains about the uses and side effects.Their antioxidant property.
Mycotoxins are strictly regulated around the world because of their strong carcinogenic effects. A simple and reliable method to analyze mycotoxins is required to ensure food safety. The current methods require time-consuming sample pretreatment. This presentation reports on a fully automated online sample extraction and analysis of mycotoxins in foods by online SFE-SFC-MS.
There is high demand for oxysterol quantitation due to their correlation with neurodegenerative diseases. The ratios of various oxysterols in biological fluids are used by researchers to study disease states. This application presents a fast, sensitive LC-MS/MS method using the LCMS-8060, with detection quantitation limits determined using multiple reaction monitoring mode for each analyte.
International Journal of Engineering Research and Applications (IJERA) is an open access online peer reviewed international journal that publishes research and review articles in the fields of Computer Science, Neural Networks, Electrical Engineering, Software Engineering, Information Technology, Mechanical Engineering, Chemical Engineering, Plastic Engineering, Food Technology, Textile Engineering, Nano Technology & science, Power Electronics, Electronics & Communication Engineering, Computational mathematics, Image processing, Civil Engineering, Structural Engineering, Environmental Engineering, VLSI Testing & Low Power VLSI Design etc.
Immunoassays have been widely used in many important areas of pharmaceutical analysis such as diagnosis of diseases, therapeutic drug monitoring, clinical pharmacokinetic and bioequivalence studies in drug discovery and pharmaceutical industries.
Investigate and Compare Chemical Compositions in Ginkgo biloba ProductsCassandra Quave
Undergraduate student research student from Quave Lab. Poster presentation given April 2016.
Ginkgo, sometimes called maidenhair tree, is the only living member of the botanical family Ginkgoaceae.
Medicinal usage was recorded in Chinese Materia Medica Ben cao gang mu by Li Shizhen in 16th Century.
Historical record: Most historical reference to the medicinal use of ginkgo refers to the seed. For example, in Ben Cao Gang Mu, ginkgo seed was cited for its use in the treatment of “ asthma, coughs, irritability of the bladder, blennorrhea, and uterine fluxes” (Read 1982).
Modern usage: the highly concentrated extract of ginkgo leaf has been primarily used to enhance mental acuity and promote peripheral circulation in dementia such as Alzheimer’s disease.
External usage: effective treatment for infectious skin diseases. A specifically characterized ginkgo leaf extract contains 24% flavonol glycosides; 6% terpene trilactones; < 5ppm ginkgolic acids.
Sensitive and selective detection of chemical residues in hops is necessary to ensure protection of consumers and the environment. Methods using LC-MS provide efficient and effective detection of chemical residues in a complex sample matrix such as hops. Presented here is an LC-MS method for detection of over 150 analytes in hops and a market survey of over 50 different hops pellets samples.
Mycotoxins are strictly regulated around the world because of their strong carcinogenic effects. A simple and reliable method to analyze mycotoxins is required to ensure food safety. The current methods require time-consuming sample pretreatment. This presentation reports on a fully automated online sample extraction and analysis of mycotoxins in foods by online SFE-SFC-MS.
There is high demand for oxysterol quantitation due to their correlation with neurodegenerative diseases. The ratios of various oxysterols in biological fluids are used by researchers to study disease states. This application presents a fast, sensitive LC-MS/MS method using the LCMS-8060, with detection quantitation limits determined using multiple reaction monitoring mode for each analyte.
International Journal of Engineering Research and Applications (IJERA) is an open access online peer reviewed international journal that publishes research and review articles in the fields of Computer Science, Neural Networks, Electrical Engineering, Software Engineering, Information Technology, Mechanical Engineering, Chemical Engineering, Plastic Engineering, Food Technology, Textile Engineering, Nano Technology & science, Power Electronics, Electronics & Communication Engineering, Computational mathematics, Image processing, Civil Engineering, Structural Engineering, Environmental Engineering, VLSI Testing & Low Power VLSI Design etc.
Immunoassays have been widely used in many important areas of pharmaceutical analysis such as diagnosis of diseases, therapeutic drug monitoring, clinical pharmacokinetic and bioequivalence studies in drug discovery and pharmaceutical industries.
Investigate and Compare Chemical Compositions in Ginkgo biloba ProductsCassandra Quave
Undergraduate student research student from Quave Lab. Poster presentation given April 2016.
Ginkgo, sometimes called maidenhair tree, is the only living member of the botanical family Ginkgoaceae.
Medicinal usage was recorded in Chinese Materia Medica Ben cao gang mu by Li Shizhen in 16th Century.
Historical record: Most historical reference to the medicinal use of ginkgo refers to the seed. For example, in Ben Cao Gang Mu, ginkgo seed was cited for its use in the treatment of “ asthma, coughs, irritability of the bladder, blennorrhea, and uterine fluxes” (Read 1982).
Modern usage: the highly concentrated extract of ginkgo leaf has been primarily used to enhance mental acuity and promote peripheral circulation in dementia such as Alzheimer’s disease.
External usage: effective treatment for infectious skin diseases. A specifically characterized ginkgo leaf extract contains 24% flavonol glycosides; 6% terpene trilactones; < 5ppm ginkgolic acids.
Sensitive and selective detection of chemical residues in hops is necessary to ensure protection of consumers and the environment. Methods using LC-MS provide efficient and effective detection of chemical residues in a complex sample matrix such as hops. Presented here is an LC-MS method for detection of over 150 analytes in hops and a market survey of over 50 different hops pellets samples.
Reserve, with complex antioxidant compounds, has very different solubility properties for each antioxidant compound.
Therefore, Reserve has been tested in both aqueous & non-aqueous solvent to analyze the antioxidant protection provided to living cells by Reserve.
The CAP-e assay is used to test whether natural products contain antioxidants capable of entering into & protecting live cells from oxidative damage. Thus, when any protective eect is seen in the CAP-e assay, it shows a biologically meaningful antioxidant protection by the product. In addition, the CAP-e assay is useful for comparing dierent production lots of the same product & for dose comparison between dierent test products or ingredients.
Estamos encantados por Você ter escolhido a Jeunesse Global® como seu parceiro.
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Analysis of Phenolic Antioxidants in Edible Oil/Shortening Using the PerkinEl...PerkinElmer, Inc.
Phenolic antioxidants are commonly used in food to prevent the oxidation of oils. Oxidized oil and fats cause foul odor and rancidity in food products, which is a major cause for concern to the food industry. Globally, regulations vary, but current maximum allowable levels are as low as 100 μg/g (100 ppm). This application note presents a UHPLC method for the analysis of the ten most common phenolic antioxidants that may be found in such products.
Determination of Etodolac in Commercial Formulations by HPLC-UV Methodijtsrd
The aim of this study was to develop and verify a simple, rapid and sensitive high performance liquid chromatography method coupled with UV detector HPLC UV method for the quantitative determination of etodolac in bulk and pharmaceutical dosage forms. Chromatographic separation was performed at ambient conditions on a reverse phase ACE C8 analytical column 250 mm x 4.6 mm ID, 5 umm using the mobile phase containing acetonitrile water 80 20, v v at a flow rate of 1.0 mL min 1. A wavelength of 272 nm was used for etodolok and paracetamol IS . A retention time of 4.21 min and 2.02 min were obtained for etodolac and IS, respectively. The method showed linearity in the range of 0.08 10 µg mL 1 for etodolac R = 0.9999 . The linear regression equations obtained by least square regression method were the ratio of peak area of etodolac and IS =1.559 concentration etodolac µg mL 0.139. The intra day and inter day RE and RSD values of the method were =10.0 and =2.65 , respectively. Limit of detection LOD and limit of quantification LOQ were found to be 0.04 and 0.06 µg mL 1 for etodolac, respectively. A new, simple and sensitive high performance liquid chromatography method was developed and validated for etodolac. The method can be applied for the quantification of etodolac without derivatization in bulk solutions and commercial formulations using the internal standard. Tugrul Cagri Akman | Yucel Kadioglu "Determination of Etodolac in Commercial Formulations by HPLC-UV Method" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-1 , December 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29452.pdfPaper URL: https://www.ijtsrd.com/pharmacy/analytical-chemistry/29452/determination-of-etodolac-in-commercial-formulations-by-hplc-uv-method/tugrul-cagri-akman
Pharmaceutical analysis (PPT) (BP102T)
In this ppt we learn about pharmaceutical analysis, introduction, scope, diffrent technique of analysis, method of expressing concentration, primary and secondary standard,
If any student are study this ppt in Hindi so Hindi and easy lecture are Provided in YouTube channel
Youtube channel link 🖇️
https://youtube.com/@PharmacyTutorial9336?si=h6vdMutP6HnT6xRF
I will provide easy tricks which helps to pramote your study.
—3-Chloro-1,2-propanediol (3-chloropropanediol) is a well-known food processing contaminant found in a wide range of foods and ingredients and there has been recent concern about the levels of carcinogenic 3-chloropropanediol (3-MCPD) in some soy sauces. This paper reports on the development of an analytical method for the fast determination of 3-MCPD at trace level in commercial soy sauce using novel liquid phase extraction (LPE)/cleanup coupled with microwave-assisted derivatization (MAD) method followed by high performance liquid chromatography-ultraviolet (HPLC-UV) detection. In this method, 3-MCPD was first isolated from soy sauce sample matrix by LPE/cleanup with Extrude NT3 column cartridges and the isolated (eluent) solution was subjected to MAD with acetophenone to form 2-methyl-2-phenyl-4-(chloromethyl)-1,3-dioxolane under microwave irradiation using a specially modified domestic microwave oven, then the derivatizeddioxolane was directly analyzed with a HPLC-UV system. The optimum conditions for MAD such as the ratio of reagents, acidic catalyst, microwave irradiation power and time, as well as the chromatographic conditions were thoroughly investigated. Experimental results indicated that maximum derivatization can be achieved in 10 min under microwave irradiation at 362 watts when compared to 18 hours by conventional refluxing reaction. The proposed method provided a simple and rapid analytical procedure for 3-MCPD analysis in soy sauce with the detection limit of 80 ng mL-1. The relative standard deviations were all below 3.0 % (n = 7). Application was illustrated by the analysis of commercial sauce sample obtained from a local traditional store in central Taiwan.
The presentation is about the chemical residues that cloud be seen in the milk. It includes the chemical residues like the antibiotic residues, pesticides, detergents and heavy metals.
Combination drugs containing Paracetamol and Aspirin, displayed in Figure 1, are widely used analgesics with anti-inflammatory properties for treatment of migraines. Both active ingredients have a similar mode of action, whereby they inhibit the cyclooxygenase (COX) enzyme, by preventing the production of prostaglandins which cause pain, inflammation, and fever. UV/Vis spectrometry is a fast and commonly used technique in quality control laboratories for routine analysis of purity and quantity of components within various stages of a product’s manufacture in many industries.
drug execipent compatibilty studies is of prime importance for the better formulation of the new drug and also for reducing cost by verfication of the data at the earlier atage.
this presentation will give the brief explanation of the goal, importance, dteps involve to studi the drug execient compatibility studies with different examples suitable accordiingly.
Poster demonstrating the results from the development/verification project for the quantitation of all- trans retinol and alpha tocopherol in human serum.
STUDY OF MPS UNDER STRESSED CONDITIONSvivatechijri
This study is done to access the chemical stability of the candidate compound in the pharmaceuticals.
Usually, it is performed at the preliminary stage in the process of drug development. Forced degradation/ stress
testing is performed under accelerated environment. The experimental conditions cause the candidate compound
to degrade under extreme conditions like acid and base hydrolysis, peroxide oxidation, photo-oxidation and
thermal stability to identify the resultant degradation products. This helps to establish degradation pathways and
thus intrinsic stability of a drug substance. The stability of product describes shelf life and storage conditions and
helps in the selection of appropriate formulations and their suitable packaging. This is compulsory for regulatory
documentation. The commonly used analytical approach for FDS is HPLC with UV and/ or MS but these
techniques consume a lot of time and not provide high resolution to confirm the precise detection of degradation
products. Use of UPLC with photodiode array and MS analysis supports the identification of degradation
products and also reduces the time needed to evolve stability indicating methods.
The Analysis of SunscreenActive Ingredients and Parabens in Lotions and Lip B...PerkinElmer, Inc.
Individuals typically use 5-20 cosmetics per day, many of which contain sunscreen to prevent skin damage from the sun’s radiation, and antimicrobial preservatives called parabens. Although sunscreen-active ingredients are designed to block UV radiation, some cell damage may be caused when these ingredients are illuminated by sunlight after absorption into the skin. For example, oxybenzone, an ingredient considered safe by the FDA (Food and Drug Administration), is believed to contribute to the recent rise in melanoma cases by increasing the production of DNA-attacking free radicals upon UV exposure. Additionally, studies have shown oxybenzone to behave similarly to the hormone estrogen, suggesting that it may also contribute to the development of breast cancer. Parabens are absorbed through the skin via cosmetic applications and can be found in nearly all adult urine samples, with the highest concentrations observed in adult females and adolescents. Furthermore, parabens are thought to have estrogenic activity, which affects the expression of genes regulated by the natural form of estrogen, leading to early puberty in girls and an increased risk for the development of breast cancer.
Application of uv visible spectroscopy in pharmaceutical industry
MultiskanFC-antioxidant-power
1. Application
Note:
AP-MIB-MSFC01-0908
An Easy Determination of the Antioxidant
Power of Beverage Samples with Thermo
Scientific Multiskan FC Microplate
Photometer
Jorma Lampinen, Arto Perälä, Reija-Riitta Harinen and Marika Raitio, Thermo Fisher Scientific Oy, Vantaa, Finland
Summary
This paper shows how antioxidant
power of different beverage
samples can be easily and rapidly
tested with a photometric assay
using Thermo Scientific Multiskan
FC microplate photometer. With
Multiskan® FC reader it is possible
to measure these antioxidants
with high sensitivity and assay
robustness. The assay is also very
fast to perform, the total time for
the complete assay is around 5 -
10 min.
Introduction
Oxidative stress has been
demonstrated to be involved
in many diseases including
atherochlerosis, Parkinson disease
and cancer. Oxidative stress is
caused by an imbalance between
the production of reactive oxygen
and a biological system’s ability to
detoxify the reactive intermediates,
or easily repair the resulting
damages. The most common
forms of reactive oxygen are free
radicals like superoxide anions,
hydroxy radicals and alkoxy or
peroxy radicals, which start chain
reactions that damage cells.
Antioxidants are molecules
capable of slowing or
preventing the oxidation of
other molecules, therefore
protecting cells from
the oxidation damages.
Antioxidants can remove
free radical intermediates,
and inhibit other oxidation
reactions by being
oxidized themselves.
Antioxidants are often
reducing agents such as
thiols or polyphenols. Commonly
known examples of antioxidants
are vitamins E and C, uric acid,
carotenes and glutathione.
This paper describes how the
total antioxidant capacity of the
unknown samples can be measured
with the photometric assay. The
assay is based on the reduction of
bivalent copper ion, Cu2+, to Cu+
by the antioxidants present on
the sample. This reduced form of
copper is then selectively bound
to chromogenic compound to
form a complex that has strong
absorbance at 490 nm. The assay
is calibrated by using a known
concentration series of standard
antioxidant.
Materials and Methods
The beverage samples used to
measure antioxidant power
were 100% natural orange and
apple juices as well as green,
black and white tea. Antioxidant
power was determined using
photometric Total Antioxidant
Power kit (Cat. no. TA 01) from
Oxford Biomedical Research Inc.
(Oxford, MI, USA) and clear flat
bottom 96-well Thermo Scientific
Immulon 1B microplates (Cat. no.
3355). The assay was performed
according to the kit instructions
except that vitamin B analogue
TroloxTM
((±)-6-Hydroxy-2,5,7,8-
tetramethylchromane-2-carboxylic
acid. Sigma-Aldrich, Cat. no.
56510) was used as a standard for
calibrating the assay.
The Trolox calibration series with
seven calibrators was prepared
to cover the concentration range
of 0.78 – 50 μM. Beverage
samples were tested with three
dilutions: undiluted, 1:5 and
1:10 dilutions. A 200 μl aliquot
of each calibrator and sample
dilution was added as duplicate
into a 96-well microplate. Then,
the background absorbance of the
plate was measured with 492 nm
with Multiskan FC controlled by
Thermo Scientific SkanIt Software
version 2.5. After that, 50 μl of the
Copper Solution was added. The
assay plate was then incubated for
three minutes at room temperature
and the reaction was stopped by
adding 50 μl of the Stop Solution.
The absorbance of the plate
was then read again at 492 nm
immediately.
The results were calculated
according to kit instructions.
Background absorbance of each
well before the addition of the
Copper Solution was subtracted
from the final absorbance values
and a linear calibration curve was
prepared based on these subtracted
2. absorbances. The antioxidant
power of each unknown sample
was then resolved from the
calibration curve as Trolox
equivalents (Trolox concentration
that has equivalent antioxidant
efficiency as the unknown sample).
Results and Discussion
All results of this Antioxidant
Power assay were calculated as
instructed in the kit insert. First,
492 nm absorbance values from
the background measurement at
the beginning were subtracted
from the result obtained after the
reagent additions using automatic
pre-calculation feature of the
SkanIt® Software. Then the linear
calibration curve was drawn
with curve fit function of the
software. The resulting calibration
curve is shown in Figure 1. Assay
robustness was analyzed using
Z-prime calculation and resulting
values are shown in figure 2.
Antioxidant power of unknown
beverage samples was claculated
automatically by SkanIt software
curve fit module. The module
calculates the concentration of
samples based on the calibration
curve and then calculates the
final result value by taking the
dilution factors into account. With
this assay, extrapolation of the
calibrator curve data was allowed
so that result values higher than
the highest calibrator could also be
calculated. These results are shown
in Table 1.
When we analyze the result data
from the measurements, one
striking observation is the excellent
precision of the reader with low
level absorbances. The background
values measured before the dye
addition have clearly below 0.001
absorbance unit standard deviation
in these blank values (n = 46). This
exceptional low level precision
improves the assay sensitivity
because absorbance values very
close to background become
statistically relevant and can be
used for the real measurements.
Both calibration curve analysis
and Z-prime analysis show that
this antioxidant power assay
is performed perfectly with
the Multiskan FC reader. The
calibration curve shows complete
linarity over whole concentration
range and Z-prime values prove
that the assay could be done even
below the concentration range
tested in this work.
When results of unknown beverage
samples are analyzed, first thing
to be noticed is that undiluted
samples clearly contain some
Figure 1. Trolox calibration curve of antioxidant power assay. Calibration curve show
the perfect correlation between amount of known antioxidant and resulting absorbance.
Calculated R2 value was 0.9999.
Figure 2. Z-prime values of antioxidant power assay. The Z-prime value is well over 0.6
over whole calibration range which proves the excellent robustness of the assay.
component disturbing the assay.
This is seen from the calculated
Trolox equivalents that were
always remarkably lower when an
undiluted sample was measured
compared to diluted samples.
Instead, two samples with different
dilution factors behave as expected
so that they gave identical results
when dilutions are taken into
account. This shows that it is
recommended to always test such
heterogenous samples with the
dilution series instead of only one
sample concentration.
Green tea was shown to be
the most powerful antioxidant