TOTAL POLYPHENOLS AND DPPH FREE RADICALS SCAVENGING ACTIVITY IN SIX LEAFY VEGETABLES OF BANGLADESH
Harun-Ar-Rashid, Sheikh Julfikar Hossain, Sk. Amir Hossain, Md Mahfuzur Rahman, Md. Kamaruzzaman
Institut Kurz specializes in performing various assays for food analysis.
In this presentation you can see some of them.
Contact: info@institut-kurz.com
Website: www.institut-kurz.com/
Various human diseases have oxidative stress as one of their component. Many herbs have been reported to exhibit properties that combat oxidative stress through their active constituents such as flavonoids, tannins, phenolic compounds etc. Different Plants of Dillenicea family has been shown in in vitro experiments to be endowed with antioxidant activity. Therefore this study was carried out to evaluate Dillenicea family for its antioxidant activity.
Evaluation of antioxidant and antiradical properties of pomegranate (punica g...Pritam Kolge
Evaluation of antioxidant and antiradical properties
of Pomegranate (Punica granatum L.) seed and defatted
seed extracts
This is Journal Club activity Presentation with the reference of various research papers.
This Presentation Contain following...
#Info about Paper
#Abstract
#Materials
#Methods
#Results
#Discussion
#Conclusion
#References
*Important Methods used
#Moisture content
#Fat content
#Acid value
#Peroxide value
#Oxidative stability index
#Total phenols content
#Preparation of Pomegranate seed extracts and calculate extract yield
#Evaluation of antioxidant properties of Pomegranate seed extracts using
-DPPH radicals scavenging activity
-FRAP assay
#Antioxidant efficiency of seed extract (Oxidative stability extract)
#Statistical analysis
Journal Club Presentation at Bharati Vidyapeeth College of Pharmacy, Kolhapur.
Thanks for Help and Guidance of Dr. P. B. Choudhari (Assistant Professor, Pharmaceutical Chemistry) and Dr. A. J. Shinde (Assistant Professor, Department of Pharmaceutics)
Institut Kurz specializes in performing various assays for food analysis.
In this presentation you can see some of them.
Contact: info@institut-kurz.com
Website: www.institut-kurz.com/
Various human diseases have oxidative stress as one of their component. Many herbs have been reported to exhibit properties that combat oxidative stress through their active constituents such as flavonoids, tannins, phenolic compounds etc. Different Plants of Dillenicea family has been shown in in vitro experiments to be endowed with antioxidant activity. Therefore this study was carried out to evaluate Dillenicea family for its antioxidant activity.
Evaluation of antioxidant and antiradical properties of pomegranate (punica g...Pritam Kolge
Evaluation of antioxidant and antiradical properties
of Pomegranate (Punica granatum L.) seed and defatted
seed extracts
This is Journal Club activity Presentation with the reference of various research papers.
This Presentation Contain following...
#Info about Paper
#Abstract
#Materials
#Methods
#Results
#Discussion
#Conclusion
#References
*Important Methods used
#Moisture content
#Fat content
#Acid value
#Peroxide value
#Oxidative stability index
#Total phenols content
#Preparation of Pomegranate seed extracts and calculate extract yield
#Evaluation of antioxidant properties of Pomegranate seed extracts using
-DPPH radicals scavenging activity
-FRAP assay
#Antioxidant efficiency of seed extract (Oxidative stability extract)
#Statistical analysis
Journal Club Presentation at Bharati Vidyapeeth College of Pharmacy, Kolhapur.
Thanks for Help and Guidance of Dr. P. B. Choudhari (Assistant Professor, Pharmaceutical Chemistry) and Dr. A. J. Shinde (Assistant Professor, Department of Pharmaceutics)
การวิเคราะห์ฤทธิ์ต้านอนุมูลอิสระที่ระยะต่างกันในกล้วยเล็บมือนาง
Analysis of Antioxidant Activity at Different Stage in Musa (AA group) ‘Kluai Leb Mu Nang’
อดิศร จำรูญ
317 - In-vitro Antioxidant studies on ethanolic extracts of Boswellia ovalifo...pharmaindexing
317 - In-vitro Antioxidant studies on ethanolic extracts of Boswellia ovalifoliolata and saccharum spontaneum by DPPH, Nitric Oxide and Lipid perooxidation methods
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Synthesis and Characterization of Schiff Base from Aromatic Amine and Aromati...ijtsrd
The synthesis of Schiff base From Aromatic Amine And Aromatic P Nitro benzaldehyde was performed by a novel method of stirring followed by the addition of p nitro benzaldehydeandm nitro aniline 0.02M . Characterization of the synthesized compounds, determination of purity and identity of the compounds using following spectroscopic and chromatographic techniques Solubility, Thin Layer Chromatographic studies, Ultra Violet studied rotational and vibrational studies FT IR studies. The compounds were investigated for their Antimicrobial activity by cup plate method. Compound1 nitro 4 1 imino,4 nitrophenyl benzene was found to be the most active according to pharmacological evaluation exhibited antimicrobial. Ms. Chetana D. Patil | Mr. Digamber N. Bhosale | Ms. Smita P. Bedis "Synthesis and Characterization of Schiff Base from Aromatic Amine and Aromatic P-Nitro Benzaldehyde" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26401.pdfPaper URL: https://www.ijtsrd.com/pharmacy/medicinal-chemistry/26401/synthesis-and-characterization-of-schiff-base-from-aromatic-amine-and-aromatic-p-nitro-benzaldehyde/ms-chetana-d-patil
Electrochemical, in-vitro in-vivo study of Co (II)-ofloxacin complexIOSR Journals
Ofloxacin complex has been synthesized and screened for its physicochemical, microbial as well as pharmacological activity have been done in solid and aqueous phase. On the basis of elemental analysis, polarographic studies, amperometric titration and IR spectral studies the probable formula for the complex has been determined at 30±1OC and ionic strength of μ= 1.0[KCl]. Raper’s paper disc method was used for microbial study against various pathogenic bacteria and fungi.Invivo syudy of Swiss mice [25-30gm] were used for antibacterial activity against ofloxacin and its complex on xyline-Alcoholic activity test Kidney, liver and serum of these rats were also studied. On the basis of observed result it could be concluded that Co(II)-Ofloxacin complex were found to be non-toxic and more potent than pure Ofloxacin.(1)
Synthesis and antimicrobial activity of some methyl (4- (benzo[d]oxazol-2-yl)...QUESTJOURNAL
ABSTRACT: In the present study, a new series of methyl (4-(benzo[d]oxazol-2-yl)phenyl) carbamodithioate amine derived from methyl (4-(benzo[d]oxazol-2-yl)phenyl) carbamodithioate (4TO1-TO6) have been synthesized by reacting the thio methyl group with different amines in presence of ethenol. The structural assessment of the compounds (TO 1- TO 6) was made on the basis of spectral data. The synthesized compounds were screened for their in vitro growth inhibiting activity against different strains of bacteria and fungi viz., Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, S. aureus, Candida albicans and Cryptococcus neoformans were compared with standard agents such as Norfloxacin (10μg/ml) and Amphotericin B (10μg/ml) using broth dilution method. Compounds exhibit moderate to high antibacterial and antifungal activity
Visible Spectrophotometric Determination of Gemigliptin Using Charge Transfer...Ratnakaram Venkata Nadh
A visible spectrophotometric method was developed and validated for the determination of gemigliptin present in bulk drug and tablet formulation. It involves an indirect method of charge transfer complex formation in presence of NBS, metol and suphanilic acid. Gemigliptin was subjected to oxidation with excess amount of oxidant (NBS) and the unconsumed NBS oxidizes metol to give p-N-methylbenzoquinone monoamine (PNMM) which in turn forms a charge transfer complex with sulphanilic acid. Then validated the above developed method as per the current ICH guidelines. An excellent correlation coefficient (> 0.999) was found for the obtained regression equation
(y = –0.0302x + 0.928) in the range of 2.0–30.0 μg mL-1. The method was found to be simple and rapid because it does not involve any solvent extraction. The recovery levels of the drug were in the range 99.92 – 100.08.
STUDIES ON TREATMENT OF PHARMACEUTICAL WASTE EFFLUENTS BY POLYMER MATERIALS M...EDITOR IJCRCPS
In the present study, sorption technique was used to achieve the optimum recovery of the pharmaceutical waste from effluents.
The modified urea formaldehyde resin was prepared and mixed with inorganic adsorbent at various proportions. The removal
capabilities of pharmaceutical waste by the prepared composite materials were investigated. Different factors affecting the uptake
such as contact time, pH value and aqueous volume to resin weight ratio have been investigated. The reaction mechanisms and
the optimum conditions for the treatment were deduced in the light of the obtained results.
Keywords: Sorption technique, Pharmaceutical waste, Different factors.
ABSTRACT- Secondary metabolites or phytochemicals from plants have eminent pharmacological activities such as
anti-oxidative, anti-allergic, antibiotic, hypoglycaemic and anti-carcinogenic. These secondary metabolites protect the
cells from the damage caused by unstable molecules known as free radicals. They can inhibit oxidation of free radicals in
both human body and food system. Food industry uses both natural and synthetic antioxidants to extend shelf life of
products. But the application of synthetic antioxidant has been limited due to its carcinogenicity. Recently research is
being focused on fruit materials, which are considered rich source of antioxidant compounds. In this study the
phytoconstituents of seed extracts of two varieties of Cucumis melo L, namely Cucumis melo cantalupensis and Cucumis
melo reticulatus, were studied for their antioxidant property by DPPH free radical scavenging method. In this
investigation, chloroform, petroleum ether, acetone, aqueous and ethanolic extracts of the fruit seed were made using cold
extraction process. Phytochemical study reveals that anthroquinones, quinines, cardiac glycosides, terpenoids, phenols and
steroids were present in aqueous extract of both the samples. The total phenolic content of their seed extracts were found
to be 8.8 mg GAE/g of dry sample and 9.2 mg GAE/g of dry sample respectively. The phenolic content was found to be
linearly proportional to the antioxidant ability of the samples.
Key-words- Cucumis melo cantalupensis, Cucumis melo reticulates, DPPH, Antioxidant, Phenolic content,
Phytochemicals
การวิเคราะห์ฤทธิ์ต้านอนุมูลอิสระที่ระยะต่างกันในกล้วยเล็บมือนาง
Analysis of Antioxidant Activity at Different Stage in Musa (AA group) ‘Kluai Leb Mu Nang’
อดิศร จำรูญ
317 - In-vitro Antioxidant studies on ethanolic extracts of Boswellia ovalifo...pharmaindexing
317 - In-vitro Antioxidant studies on ethanolic extracts of Boswellia ovalifoliolata and saccharum spontaneum by DPPH, Nitric Oxide and Lipid perooxidation methods
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Synthesis and Characterization of Schiff Base from Aromatic Amine and Aromati...ijtsrd
The synthesis of Schiff base From Aromatic Amine And Aromatic P Nitro benzaldehyde was performed by a novel method of stirring followed by the addition of p nitro benzaldehydeandm nitro aniline 0.02M . Characterization of the synthesized compounds, determination of purity and identity of the compounds using following spectroscopic and chromatographic techniques Solubility, Thin Layer Chromatographic studies, Ultra Violet studied rotational and vibrational studies FT IR studies. The compounds were investigated for their Antimicrobial activity by cup plate method. Compound1 nitro 4 1 imino,4 nitrophenyl benzene was found to be the most active according to pharmacological evaluation exhibited antimicrobial. Ms. Chetana D. Patil | Mr. Digamber N. Bhosale | Ms. Smita P. Bedis "Synthesis and Characterization of Schiff Base from Aromatic Amine and Aromatic P-Nitro Benzaldehyde" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26401.pdfPaper URL: https://www.ijtsrd.com/pharmacy/medicinal-chemistry/26401/synthesis-and-characterization-of-schiff-base-from-aromatic-amine-and-aromatic-p-nitro-benzaldehyde/ms-chetana-d-patil
Electrochemical, in-vitro in-vivo study of Co (II)-ofloxacin complexIOSR Journals
Ofloxacin complex has been synthesized and screened for its physicochemical, microbial as well as pharmacological activity have been done in solid and aqueous phase. On the basis of elemental analysis, polarographic studies, amperometric titration and IR spectral studies the probable formula for the complex has been determined at 30±1OC and ionic strength of μ= 1.0[KCl]. Raper’s paper disc method was used for microbial study against various pathogenic bacteria and fungi.Invivo syudy of Swiss mice [25-30gm] were used for antibacterial activity against ofloxacin and its complex on xyline-Alcoholic activity test Kidney, liver and serum of these rats were also studied. On the basis of observed result it could be concluded that Co(II)-Ofloxacin complex were found to be non-toxic and more potent than pure Ofloxacin.(1)
Synthesis and antimicrobial activity of some methyl (4- (benzo[d]oxazol-2-yl)...QUESTJOURNAL
ABSTRACT: In the present study, a new series of methyl (4-(benzo[d]oxazol-2-yl)phenyl) carbamodithioate amine derived from methyl (4-(benzo[d]oxazol-2-yl)phenyl) carbamodithioate (4TO1-TO6) have been synthesized by reacting the thio methyl group with different amines in presence of ethenol. The structural assessment of the compounds (TO 1- TO 6) was made on the basis of spectral data. The synthesized compounds were screened for their in vitro growth inhibiting activity against different strains of bacteria and fungi viz., Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, S. aureus, Candida albicans and Cryptococcus neoformans were compared with standard agents such as Norfloxacin (10μg/ml) and Amphotericin B (10μg/ml) using broth dilution method. Compounds exhibit moderate to high antibacterial and antifungal activity
Visible Spectrophotometric Determination of Gemigliptin Using Charge Transfer...Ratnakaram Venkata Nadh
A visible spectrophotometric method was developed and validated for the determination of gemigliptin present in bulk drug and tablet formulation. It involves an indirect method of charge transfer complex formation in presence of NBS, metol and suphanilic acid. Gemigliptin was subjected to oxidation with excess amount of oxidant (NBS) and the unconsumed NBS oxidizes metol to give p-N-methylbenzoquinone monoamine (PNMM) which in turn forms a charge transfer complex with sulphanilic acid. Then validated the above developed method as per the current ICH guidelines. An excellent correlation coefficient (> 0.999) was found for the obtained regression equation
(y = –0.0302x + 0.928) in the range of 2.0–30.0 μg mL-1. The method was found to be simple and rapid because it does not involve any solvent extraction. The recovery levels of the drug were in the range 99.92 – 100.08.
STUDIES ON TREATMENT OF PHARMACEUTICAL WASTE EFFLUENTS BY POLYMER MATERIALS M...EDITOR IJCRCPS
In the present study, sorption technique was used to achieve the optimum recovery of the pharmaceutical waste from effluents.
The modified urea formaldehyde resin was prepared and mixed with inorganic adsorbent at various proportions. The removal
capabilities of pharmaceutical waste by the prepared composite materials were investigated. Different factors affecting the uptake
such as contact time, pH value and aqueous volume to resin weight ratio have been investigated. The reaction mechanisms and
the optimum conditions for the treatment were deduced in the light of the obtained results.
Keywords: Sorption technique, Pharmaceutical waste, Different factors.
ABSTRACT- Secondary metabolites or phytochemicals from plants have eminent pharmacological activities such as
anti-oxidative, anti-allergic, antibiotic, hypoglycaemic and anti-carcinogenic. These secondary metabolites protect the
cells from the damage caused by unstable molecules known as free radicals. They can inhibit oxidation of free radicals in
both human body and food system. Food industry uses both natural and synthetic antioxidants to extend shelf life of
products. But the application of synthetic antioxidant has been limited due to its carcinogenicity. Recently research is
being focused on fruit materials, which are considered rich source of antioxidant compounds. In this study the
phytoconstituents of seed extracts of two varieties of Cucumis melo L, namely Cucumis melo cantalupensis and Cucumis
melo reticulatus, were studied for their antioxidant property by DPPH free radical scavenging method. In this
investigation, chloroform, petroleum ether, acetone, aqueous and ethanolic extracts of the fruit seed were made using cold
extraction process. Phytochemical study reveals that anthroquinones, quinines, cardiac glycosides, terpenoids, phenols and
steroids were present in aqueous extract of both the samples. The total phenolic content of their seed extracts were found
to be 8.8 mg GAE/g of dry sample and 9.2 mg GAE/g of dry sample respectively. The phenolic content was found to be
linearly proportional to the antioxidant ability of the samples.
Key-words- Cucumis melo cantalupensis, Cucumis melo reticulates, DPPH, Antioxidant, Phenolic content,
Phytochemicals
Phytochemical Evaluation and Antioxidant Activity of Holarrhena pubescens Wal...BRNSS Publication Hub
The selected medicinal plant Holarrhena pubescens Wall. ex G.Don belongs to the family Apocynaceae, and it was collected in hillock of Muthu Malai hill in Coimbatore, Tamil Nadu. In the present study, preliminary phytochemical screening of H. pubescens a medicinal plant was carried out. Qualitative phytochemical analysis of these plants confirms the presence of various secondary metabolites such as steroids, tannins, alkaloids, and phenols. The results suggest that the phytochemical properties for curing various ailments possess potential anti-inflammatory, antimicrobial, and antioxidant and leads to the isolation of new and novel compounds. Gas chromatography-mass spectrometry analysis showed the existence of various compounds with different chemical structures. The presence of various bioactive compounds confirms the application of H. pubescens for various ailments by traditional practitioners. However, isolation of individual phytochemical constituents may proceed to find a novel drug. Extracts from H. pubescens showed varying antioxidant (free radical scavenging) activities when compared to Vitamin C, and the results suggest that the antioxidant activity of H. pubecens may contribute to their claimed medicinal property.
Healing Effects of Hydroalcoholic Extract of Guava (Psidium guajava) Leaf on ...Dr. Anuj S Parihar
Oral mucositis (OM) is a common inflammatory complication among cancerous patients as an adverse effect of chemotherapy and radiotherapy. The aim of this study is to evaluate the healing effects of hydroalcoholic extract of Psidium
Guajava leaf on oral induced mucositis induced by 5-fluorouracil using histopathologic and tissue antioxidative markers assessment in male dark brown rats. In a prospective randomized double blind animal study, OM was induced in 64 male dark brown rats that allocated in 4 groups by 5-FU (60 mg/kg) on days 0, 5, and 10 of the study. The cheek pouch was scratched with a sterile needle on once daily on days 3 and 4. Starting from day 12, gel base, topical form and 600 mg/kg dietry form of hydroalcoholic extract of Psidium Guajava leaf were administered per day. Pouch histopathology score, superoxide dismutase, glutathione peroxidase, total antioxidant capacity were evaluated on day 14 and 18. DPPH scavenging activity and total phenolic content also were measured. Histopathology scores of mucositis were lower in the systemic and topical treatment groups than the gel base and control groups (P<0.05). Higher activities of SOD, GPX and TAC were detected in the topical and systemic treatment groups in comparison to the others (P<0.05). The extract was rich in total phenolic content as antioxidant. The use of extract of Psidium Guajava leave may be associated with reduced intensity of OM, increased concentration of SOD, GPX and TAC on induced
OM in dark brown rats undergoing 5-FU consumption.
Evaluation of Antioxidant Phytochemicals in Different Genotypes of PotatoIJERA Editor
Potato (Solanum tuberosum L.) is considered a natural source of phytochemicals that help lower the risk of chronic diseases. Thus, ascorbic acid, total phenolic content and antioxidant activities of Indian potato genotypes were studied. ‘Kufri Pukhraj’ was the best genotype in terms of nutritional components, since it contained the highest amount of ascorbic acid (19.42 mg/100g), and total phenolic content (68.20 mg GAE/100g). Also ‘Kufri Pukhraj’ had the highest DPPH radical scavenging activity of 67.30%. On the other hand, genotype ‘Kufri Chipsona-1’, most commonly used cultivar for processing, displayed the lowest content of total phenolics (31.30 mg GAE/100g) and total antioxidant activity (28.80%). Correlation analysis between total phenolic content and antioxidant activity (r=0.8502) showed a high degree of correlation indicating that these bioactive components contributes to the total antioxidant activity in potato genotypes.
Genetic Variability for Antioxidant Activity and Total Phenolic Content in Fo...CrimsonpublishersNTNF
Total phenolic content and antioxidant activity were evaluated in 139 diverse genotypes of four pulse crops including 54 genotypes of chickpea (Cicer arietinum), 37 lentil (Lens culinaris), 21 pigeonpea (Cajanus cajan), and 26 blackgram (Vigna mungo). Results indicate significant genotypic variation (p < 0.01) for total phenolic content (TPC) as well as antioxidant activity (AOA). Amongst the four major pulse crops tested, maximum mean phenolic content was recorded in blackgram genotypes (7.01mg GAE/g), followed by lentil (3.46mg GAE/g), pigeonpea (3.32mg GAE/g) and chickpea (2.30mg GAE/g). In general, the Mediterranean landraces of lentil had higher phenolic content as compared to the other lentil varieties and breeding lines. Amongst the chickpea genotypes the phenolic content ranged from 0.40 to 5.63mg GAE/g and comparatively higher value for phenolic content was recorded in desi types (2.67mg GAE/g) as compared to the Kabuli types (1.05mg GAE/g). The antioxidant activity (AOA) was assayed in mature dry seeds utilizing DPPH (2,2-Diphenyl-1-picryl hydrazyl) radical scavenging assay which ranged from 1.73 to 19.14μmole Trolox/g tissue. As observed for TPC, highest AOA was also recorded in blackgram genotypes (19.14μmole Trolox/g tissue), followed by pigeonpea (2.72μmole Trolox/g tissue), chickpea (2.05μmole Trolox/g tissue) and lentil (1.73μmole Trolox/g tissue). Highly significant genotypic as well as phenotypic correlation (p<0.01) was recorded between phenolic content and antioxidant activity in chickpea, lentil as well as blackgram (rG values ranging from 0.268 to 0.850 and rP from 0.253 to 0.817), however, surprisingly the values were non-significant in case of pigeonpea. Strongest genotypic correlation was recorded in chickpea (rG=0.850), followed by lentil (rG =0.744), and blackgram (rG =0.268). High broad-sense heritability (h2bs) (0.89 to 0.97) for phenol content was recorded which indicates that substantial portion of total variation for phenolic content is due to genetic effects.
Comparative Assessment of Total Polyphenols and Antioxidant Activity of Comme...AnuragSingh1049
Green Tea, made from Camellia sinensis plant leaves, is one of the most popular drinks in the world. For the past decades, scientists have studied this plant in terms of potential health benefits. Research has shown that green tea helps prevent stroke, malignancy and infections. In this paper, antioxidant activity and total phenol content of 4 samples of green tea from local Tuzla stores were investigated, of which two were of foreign origin. The antioxidant activity of the samples was analyzed using FRAP and DPPH methods. The obtained results show that the highest content of total phenols and the largest antioxidant capacity has a sample of foreign origin. The content of total phenols in the samples ranges from 60.01 to 79.34 mg GAE/g. The highest FRAP value is 3.34 mmol/g. The antioxidant capacity was also confirmed by the DPPH method. The IC50 value ranges from 0.014 to 0.030 mg/mL.
Phytochemical, Antioxidant and Antibacterial Studies on Bambusa arundinacea a...SUS GROUP OF INSTITUTIONS
This study was formulated to check the phytochemical, antioxidant, antibacterial potential of
Bambusa arundinacea (Bamboo) and Mangifera indica (Mango) trees. Aqueous, ethanolic and
methanolic extracts were prepared from leaves of former and stem bark of later. The phytochemical
screening of the extracts showed the presence of various bioactive compounds such as
carbohydrates, flavonoids, saponins and proteins in B. arundinacea, alkaloids, flavonoids, tannins,
saponins, steroids and cardiac glycosides in M. indica. Total phenolic concentration and
percentage of free radical scavenging activity was more in ethanolic extracts of B. arundinacea and
M. indica followed by methanolic extracts and aqueous extracts. Highest percentage of ferric
reducing antioxidant power was found in ethanolic extracts and lowest in aqueous extracts indicates
that ethanolic extracts has more antioxidant potential than the other two extracts. Ethanolic extracts
of both plants had higher inhibition on the tested Gram positive (B. subtilis & S. aureus) as well as
Gram negative (E.coli & P. aeruginosa) bacteria evidenced from the zones of inhibition. M. indica
showed more therapeutic potential as compared to B. arundinacea and ethanolic as well as
methanolic extracts of both the tested plants were more effective than aqueous extracts due to better
extraction power of organic solvents. Overall study indicates that B. arundinacea and M. indica are
potential source of natural antioxidants, phytochemicals and antibacterials that can be used for the
development of novel drugs and may represent new source of antimicrobials with stable, biologically
active components that can establish a scientific base for further use in modern medicines.
Phytochemical composition and antiradical activity of Sakersia africana Hook....Open Access Research Paper
The valorization of the medicinal plants of our country and determination of their impact on health due to their abundance of substances with various pharmacological effects are our principal objective. This study was evaluated the phytochemical screening and radical 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of different extracts of Sakersia africana Hook. f.. The results revealed that Sakersia africana Hook. f. is rich in phenols compounds, sterols, triterpenes, alkaloids and reducing compound. The values in total phenols and proanthocyanidines are ranging respectively from 391.58 ± 0.04 to 777 ± 0.03 mg/100 g of drugs and 113.5 ± 3.17 to 653.5 ± 36.83 mg/100 g of drugs. Results also show that different extracts tested present antiradical activity with values of IC50 ranging from 164.21± 0.014 to 195.54± 0.012 % and abundance in bioactive compounds. This study could justify the use of Sakersia africana of some chronic diseases.
in vitro study on total phenols and flavonoids content and dpph activity of w...IJEAB
The escalating interest in appraisal of antioxidant power of herbal plant as medicine, the current study was carried out to explore the antioxidant potential of aqueous extracts of Withania somnifera root and Withania coagulan fruit in-vitro. Antioxidant activity; total phenol,total flavonoids and DPPH free radical scavenging assay of Withania somnifera root and Withania coagulans fruit aqueous extracts were determined by using reference standards gallic acid, quercetin and ascorbic acid, respectively. The highest total phenols content (mgGAE/g) and total flavonoids content (mgQE/g) was found to be 33.1±0.82 and 1.86±0.01 respectively in aqueous somnifera root extracts as compared to coagulans fruit extract . The DPPH radical scavenging activity of the both extracts was increased with the increasing concentration and was observed high in aqueous extract insomniferaroot (IC50= 54) than coagulans fruit (69μg/ml) aqueous extract.Thus,Withania somnifera root has potent antioxidant activity and may serve as a good pharmacotherapeutic agent which could be explored to provide affordable medicines to masses.
Antioxidant and-anticancer-activities-of-moringa-leavesSilentdisco Berlin
Moringa is a plantfood of high nutritional value, ecologically and economically beneficial and readily available in the countries hardest hit by the food crisis. http://miracletrees.org/ http://moringatrees.org/
Phytochemical and antioxidant studies of methanol and chloroform extract from...shailendradhakal
This research was carried out with the aim of phytochemical analysis and determining antioxidant activity present in methanol and chloroform leaf extracts of Azadirachta indica. Due to its potential in curing various ailments as well as wide spread application of antioxidant activity such as in the field of cosmetology, the plant was selected for the study. The total phenolics contained in the plant extracts were also studied which are responsible for the antioxidant activity.
COMPARSION OF ANTIOXIDANT POTENTIAL OF DIMOCARPUS LONGAN LOUR. EXTRACTS AND ...IJSIT Editor
The present study was carried out to evaluate antioxidant activity of Dimocarpus longan stems
extracts and also to investigate the main phytoconstituents in the bio-active extract. N-hexane,
dichloromethane, ethyl acetate and methanol 80% extract were tested for free radical scavenging activity on
model reaction with stable 2,2-diphenyl-1-picrylhydrazyl radical (DPPH). The results showed that ethyl
acetate was the most active one as antioxidant agent and phytochemical analysis of that extract revealed the
presence of triterpenes, flavonoids, tannins and carbohydrates. The results may help to discover new
chemical classes of natural antioxidant substances that could serve as selective agents for infectious diseases.
Forecasting Model for leaf Spot of Banana by Md. Kamaruzzaman Md. Kamaruzzaman
A model is nothing but a sequential hypothesis of what to do before epidemic .It is the simple and easy expression of forecasting information. It can be expressed by graphically or diagrammatically.
A brief Study on Quality Control Attributes of Agricultural Inputs (seeds and...Md. Kamaruzzaman
Bangladesh is primarily an agriculture based country with agriculture accounting for 23.50% of the country’s GDP. About 62% of the people are engaged in agricultural product. Previous some decades Bangladesh is importing huge amount of rice, vegetables, fruits seed including hybrid variety and fertilizer from foreign countries. In Bangladesh only 10% of seeds used for crop production are produced by the government or semi government organizations. The rest (90%) of total requirements of the country are met by the farmer’s own seed of unknown quality with respect to quality and health standard. As many as quality less and 490 seed-borne diseases affecting the seed of 76 different important crops are established in the country and these cause yield losses annually. On the other hand adulated fertilizer may responsible for economic loose with decreasing soil fertility. Poor or quality less fertilizer has significant effect on our overall production. So, role of seed quality and fertilizers are vice versa. Development of quick and cheap earthen dish-newsprint technique of seed and fertilizer quality determination by using physical and chemicals methods have been described. Measures to overcome the activity, problems are discussed in this report. Standard storage condition both seeds and fertilizers are discussed.
IDM MODEL OF RED ROT OF SUGERCANE By Md. Kamaruzzaman ShakilMd. Kamaruzzaman
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TOTAL POLYPHENOLS AND DPPH FREE RADICALS SCAVENGING ACTIVITY IN SIX LEAFY VEGETABLES OF BANGLADESH 187-190(ajpr) BY MD. KAMARUZZAMAN
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Asian J. Pharm. Res. Vol 5, Issue 3, 187-190, 2015.
e-ISSN 2231 – 363X
Print ISSN 2231 – 3621
TOTAL POLYPHENOLS AND DPPH FREE RADICALS
SCAVENGING ACTIVITY IN SIX LEAFY VEGETABLES OF
BANGLADESH
Harun-Ar-Rashid1
*, Sheikh Julfikar Hossain1
, Sk. Amir Hossain1
, Md Mahfuzur Rahman1
,
Md. Kamaruzzaman2
1
Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh.
2
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
ABSTRACT
Vegetables are the most important sources of essential bioactive compounds providing health benefits. To seek out the
potential cheap sources of dietary bioactive compounds, ethanol extracts of six commonly consumed Bangladeshi leafy
vegetables were screened for polyphenols and DPPH free radical scavenging activity. Among the extracts, Lagenaria siceraria
showed the highest total polyphenol content (21.45 mg gallic acid equivalent (GAE)/g extract), followed by Basella alba (15.51
mg GAE/g) and Coriander sativum (14.37 mg GAE/g) whereas Centella asiatica showed the lowest polyphenol content (9.62
mg GAE/g extract), aftreward Chenopodum album (12.93 mg GAE/g) and Pisum sativum (13.17 mg GAE/g). Pisum sativum
showed the most potent DPPH free radical scavenging activity with an IC50 76.64 µg/ml subsequently Lagenaria siceraria
123.78 µg/ml. From the given results it can be concluded that Pisum sativum followed by Lagenaria siceraria are most
potential sources of antioxidants among the six leafy vegetables.
Key words: Leafy vegetables, Total polyphenols, DPPH, Ethanol extracts.
INTRODUCTION
In Bangladesh, Vegetables are the cheapest and
more available food items of daily diet. But most of the
peoples are not conscious about the nutritional value of
these vegetables because of the lack of research and
specific data.
Polyphenols are the Secondary metabolites mainly
secretes by plants known as phytochemicals may have
beneficial effects on human health and provide protection
against such chronic diseases as cardiovascular diseases,
neurodegenerative disorders, and cancers [1-5].
DPPH is purple colored, stable free radical, reduced
into the yellow-colored diphenylpicryl hydrazine. DPPH is
widely used to test the ability of compounds to act as free
radicals scavengers or hydrogen donors and to evaluate
antioxidant activity of foods [6].
The various methods used to measure antioxidant
activity of food products can give varying result depending
on the specificity of the free radical being used as a
reactant. The DPPH method can be used for solid or liquid
samples and is not specific to any particular antioxidant
components, but applies to the overall antioxidants
capacity of the sample.
Bangladesh is bestowed with gentle climatic
condition conducive for the growth of different leafy
vegetables known for their nutritional values. A measure of
the total polyphenols and DPPH free radical activity will
help us to understand the functional properties of these
vegetables. Ex:…for their nutritional value. A measure of
the……..of these vegetables. So this research will be
helpful to understand the nutritional value of the six leafy
vegetables. The objectives of this study to determine the
total phenolic content and also the DPPH free radical
scavenging ability of the six leafy vegetables.
MATERIALS AND METHODS
Collection and preparation of plant materials
The leafy vegetables of Centella asiatica,
Chenopodum album, Coriander sativum, Pisum sativum,
Lagenaria siceraria and Basella alba were collected from
Corresponding Author :- Harun-Ar-Rashid Email:- harun_07bge@yahoo.com
1
Asian Journal
of
PHARMACEUTICAL RESEARCH
Journal homepage: - www.ajprjournal.com
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the local market of Khulna city, Bangladesh. After washing
with distilled water the collected vegetables were shed
dried. The dried sample was ground to powder by grinding
machine and stored in air tight containers. Ethanol was
used as solvent.
Twenty five grams of powder of each sample was
taken in 100 ml of ethanol and kept in airtight bottle. After
7 days, the ethanol was filtered by Whatman No. 1 filter
paper. The filtrate was air dried and the solid extracts were
kept in the refrigerator. For the preparation of lipophilic
extract, 10 g powder was shaken vigorously by hand with
100 ml of 100% ethanol and after filtration the filtrate was
air dried to obtain ethanol extract. Finally 10 mg of the
solid extract was dissolved in 1ml of 100% ethanol used to
conduct the experiments.
Determination of Total phenolic content (TPH)
The total concentration of phenolics (TPH) in ethanol
extracts was determined (in triplicate) according to the
Folin-Ciocalteu method [7] with gallic acid (GA) as the
standard and expressed (mg) as gallic acid equivalents
(GAE)/g of extract [8].
Determination of DPPH free radical scavenging activity
The reaction mixture (total volume, 3 ml), consisting
of 0.5 ml of 0.5 M acetic acid buffer solution at pH 5.5, 1
ml of 0.2 mM DPPH in ethanol, and 1.5 ml of 50% (v/v)
ethanol aqueous solution, was shaken vigorously with the
ethanol extracts [9]. After incubation at room temperature
for 30 min, the amount of DPPH remaining was
determined by measuring the spectrophotometer with
absorbance at 517 nm. Mean values were obtained from
triplicate experiments.
Results are expressed as mean ± SD for a given
number of observations (n = 3). The level of significance
was set at p value of 0.05.
RESULTS
Total phenolic content (TPH)
Total Phenolic Contents (TPH) of ethanolic extract of
six leafy vegetables was shown in Table 1. The TPH
content was expressed as gallic acid (GA) equivalent. The
TPH content ranged from 9.62 to 21.45 mg GAE/g of
extract. The amount was highest in the extract of L.
siceraria (21.45 mg GAE/g) followed B. alba (15.51 mg
GAE/g) and C. sativum (14.37 mg GAE/g) whereas it was
lowest in the extract of C. asiatica (9.62 mg GAE/g)
followed by C. album (12.93 mg GAE/g) and P. sativum
(13.17 mg GAE/g).
DPPH Free Radical Scavenging Activity
The DPPH free radical scavenging activities of six
leafy vegetables are also shown in Table 1. The DPPH free
radical scavenging ranged from 46.17 to 92.84 % at 200
µg/ ml of extract. At this concentration P. sativum showed
the highest DPPH radical scavenging activity (92.84%)
followed by L. siceraria (84.84%) and B. alba (71.63%).
C. album exerted the lowest activity (46.17%).We also
done the dose-dependent DPPH free radical scavenging
activity of highest two value at 25, 50,100,150 and
200µg/ml concentration by the ascorbic acid (AA)
standard. The dose-dependent DPPH free radical
scavenging activity of P. sativum and L. siceraria are
shown in Fig .1. The DPPH free radical scavenging
activity was increased by the increasing of the
concentration of the two vegetables. The comparison of
IC50 values for DPPH free radical scavenging activity of
the two most potent leafy vegetables are shown in Fig. 2.
Here we also found the positive result. For P. sativum was
76.64µg/ml and for L. siceraria was 123.78µg/ml. The
correlation between total polyphenols and DPPH free
radical scavenging activity is shown in Fig. 3. The
correlation co- efficient r is 0.57.
Fig 1. Dose-dependency of DPPH free radical scavenging
activity of P. sativum and L. siceraria (n=3).
Fig 2. Comparison of IC50 values of P. sativum and L.
siceraria. The lowest value shows the highest activity
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Fig 3. Correlation between total polyphenols and DPPH free radical scavenging activity at 200 µg/ml of extracts of
Leafy vegetables (r = 0.57)
Table 1. Total phenolic content (TPH) and DPPH Free radical scavenging activity of the ethanolic extracts of six leafy
vegetables .The data are presented as mean ±SD of at least three experiments (n=3)
Name of the Sample Total phenolic content (mg GAE/g)
(%) DPPH Free Radical Scavenging Activity
(200µg/ml)
B. alba 15.51 ± 0.70 71.63 ± 0.57
C. album 12.93 ± 0.58 46.17 ± 1.06
C. asiatica 9.62 ± 0.43 61.43 ± 1.13
C. sativum 14.37 ± 0.65 68.91 ± 1.65
L. siceraria 21.45 ± 0.97 84.84 ± 1.41
P. sativum 13.17 ± 0.59 92.84 ± 0.11
GAE=Gallic acid equivalent, SD=Standard deviation, n=no of experiment
DISCUSSION AND CONCLUTION
Based on my experimental results among the six leafy
vegetables, L. siceraria showed the highest content of total
polyphenols followed by B. alba, C. sativum, P. sativum,
C. album and C. asiatica. Whereas the P. sativum can
scavenge the large amount of DPPH free radical followed
by L. siceraria, B. alba, C. sativum, C. asiatica ,C. album.
From the comparison of the two tests we suggest that from
among the six leafy vegetables L. siceraria and P. sativum
are the most potent two leafy vegetables that are related to
Hossain et al [8].
For the confirmation of our result we also done other
related experiment of the two most potent vegetables .We
also done the dose dependent test of the P. sativum and L.
siceraria .The experiment was conducted at four different
concentrations; 200µg/ml, 100µg/ml, 50µg/ml, 25µg/ml.
The DPPH free radical scavenging activity increased due
to the increased of the concentration that proved by
Hossain et al [8]. The IC50 value of ethanolic extract of
P.sativum was found as 76.64 μg/ml, while the IC50 value
of L. siceraria was found as 123.78µg/ml.The low IC50
value of ethanolic extract of P. sativum is due to presence
of high polyphenols in it.
Hossain et al [8] conducted an experiment on
Bangladeshi fruits and correlation co- efficient between
total polyphenol and DPPH radical scavenging activity was
0.73. In our experiment, the correlation co-efficient
between total polyphenol and DPPH radical scavenging
activity is 0.57. Probably unknown components rather than
polyphenols present in the extracts of vegetables might
contribute in part to their antioxidant activity. To take the
all consideration of our result we can grade our vegetables
according to the antioxidant potentiality P. sativum> L.
siceraria> B. alba> C. sativum> C. album>C. asiatica.
Besides, due to complexity of oxidation-anti-
oxidation process, no single testing method is capable of
providing a complete anti-oxidative profile [10]. Therefore
a multi-method approach is required to asses a complete
anti-oxidative activity. This study was an attempt to
determine the total phenolic content and DPPH free radical
scavenging activity, dose-dependency of DPPH free
radical scavenging activity and correlation between total
polyphenol and DPPH free radical scavenging activity.
ACKNOWLEDGEMENT
This research work was supported by the grant from
the Environmental and Nutraceuticals laboratory, Khulna
University which is gratefully acknowledged.
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REFERENCES
1. Arts ICW and Hollman PCH. Polyphenols and disease risk in epidemiologic studies. The American Journal of Clinical
Nutrition, 81, 2005, 317–25.
2. Chun OK, Chung SJ and Song WO. Estimated dietary flavonoid intake and major food sources of U.S. adults. Journal of
Nutrition, 137, 2007, 1244–52.
3. Erdman JW, Balentine D, Arab L, Beecher G, Dwyer J, Folts J, Harnly J, Hollman P and Keen C. Flavonoids and heart
health: proceedings of the ILSI North America Flavonoids Workshop, May 31-June 1, 2005, Washington, DC. Journal of
Nutrition, 137, 2007, 718–737.
4. Williamson G and Manach C. Bioavailability and bioefficacy of polyphenols in humans. II. Review of 93 intervention
studies. The American Journal of Clinical Nutrition, 81, 2005, 243–55.
5. Prior RL and Wu X. Anthocyanidins: structural characteristics that result in unique metabolic patterns and biological
activities. Free Radical Research, 40, 2006, 1014–28.
6. Vinson JA, Hao Y and Zubik SK. Food Antioxidant quantity and quality in foods and vegetables. Journal of Agricultural
and Food Chemistry, 46, 1998, 3630-3634.
7. Ough CS and Amerine MA. Methods for analysis of musts and wine. Wiley & Sons, New York, 1988, 400.
8. Hossain SJ, Tsujiyama I, Takasugi M, Islam MA, Biswas RS and Aoshima H. Total phenolic content, antioxidative, anti-
amylase, anti-glucosidase, and antihistamine release activities of Bangladeshi fruits. Food Science and Technology
Research, 14, 2008, 261-268.
9. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature, 181, 1958, 1199-1200.
10. Parejo I, Viladomat F, Bastida J, Rosas-Romero, Flerlage N, Burillo J and Codina C.Comparison between the radical
scavenging activity and antioxidant activity of six distilled and non distilled mediterranean herbs and aromatic plants.
Journal of Agricultural and Food Chemistry, 50, 2002, 6882-6890.