This document discusses the use of nanotechnology in horticultural pest management. It begins with definitions of nanotechnology and nanopesticides. Some key formulations discussed include nanoemulsions, nanoencapsulation, and nanoparticles. Examples are provided of research using nanoemulsions containing plant extracts that showed insecticidal effects. Another study discusses the use of pheromone-loaded nanogels for fruit pest management that lasted up to a month. A third study found that chitosan nanoparticles loaded with a pesticidal protein had higher mortality effects on a major groundnut pest compared to the free protein. The document concludes with discussing the potential of DNA-tagged nano gold as a tool for armyworm control.

1. BIGGEST REVOLUTION BY
THE SMALLEST SCIENCE
Welcome to
NANO WORLD

2. Impact of Nano formulation
in Horticultural crop Pest
Management
V. G O W T H A M
2 0 1 6 6 0 0 5 0 4
A G R L . E N TO M O L O G Y
TA M I L N A D U A G R I C U LT U R A L U N I V E R S I T Y

3. Nanotechnology…?
“Nanotechnology is the art and
science of manuplating matter at
nano scale’’
 “Nanotechnology refers to
controlling, building, and
restructuring materials and
devices on the scale of atoms and
molecules”.
 A nanometer (nm) is one-
billionth of a meter.

4. Nanopesticides
Nano-scale either active ingredients with a particle
size of 100 nm or less
Formulation of a pesticides
 Nano emulsion
 Nano suspension
 Nano encapsulation
 Nanoparticles

5. Nano Emulsion
 Consist of lipid or polymeric vesicles or particles
 Size 20-200nm
 Larger surface area, slower release
 Non sedimentation or creaming

7. Development of an insecticidal nanoemulsion
with Manilkara subsericea (Sapotaceae) extract
 Fernandes et al. 2014
 Journal of Nanobiotechnology 2014, 12:22

8.  Hexane-soluble fraction from ethanolic crude extract
from fruits of Manilkara subsericea and its
triterpenes were considered active against a cotton
pest (Dysdercus peruvianus).
 poor water solubility, including triterpenes
 A nano emulsion prepared
 5% of oil (octyldodecyl myristate),
 5% of surfactants (sorbitan monooleate/polysorbate
80),
 5% of apolar fraction from M. subsericea
 85% of water.

9. Nano Encapsulation
 Encapsulation packaging the nano-scale active
ingredient within a kind of tiny “ envelope or shell”
 Nanotechnology enables us to manipulate the
properties of the outer shell of the capsule.

10. Uses
 Protection
 Increase solubility
 Reduce the contact of active ingredients with agriculture
workers
 Environments reducing run off rates
Mohdzobir et al., 2011

12. Efficient Management of Fruit Pests by Pheromone
Nanogels
 Deepa Bhagat et al.,
 Scientific Reports | 3 : 1294 | DOI: 10.1038/srep01294
 2013

13. Pheromones
• Nanogel prepared using methyl eugenol (ME)+low-
molecular mass gelator
• Very stable at ambient condition
• works well in rainy season
• Last for month

14. (Deepa Bhagat,2013)

15. Slope magnitude comparison
(Deepa Bhagat,2013)

16. (Deepa Bhagat,2013)
30 days after storage
Fruit flies attracted towards the thin film nanogel

17. 1 2
3 4
Nanogel
Fruit fly counting
Establishment
(Deepa Bhagat,2013)
Field experiment

18. Numberofcatches
Number of days
(Deepa Bhagat,2013)
Number of days of catches for the ME and Nano ME gel

19. Nano suspension
 Submicron colloidal dispersion of pure active
compounds typically range from 50-500nm.
 Improvement of efficacy due to higher surface area.
 Higher solubility, higher mobility.
 Induction of systematic acivity due to smaller
particle size.

21. Nanoparticles
 Solid core or matrix, usually composed of metallic
atom
 Material used for the fabrication Metal oxide certain
and silicates, magnetic material.

22.  Biocompatible Chitosan Nanoparticles Incorporated
Pesticidal Protein Beauvericin (CSNp-BV) Preparation for
the Improved Pesticidal Activity Against Major Groundnut
Defoliator Spodoptera Litura (Fab.) (Lepidoptera;
Noctuidae)
 Arvind Bharani et al.,
 International Journal of ChemTech Research,2014

23. Cumulative mortality (%) of S.litura treated with free beauvericin (F-BV)
and chitosan nanoparticles incorporated beauvericin (CSNp-BV)
(Arvind Bharani, 2014)

24. Effect of free beauvericin (F-BV) and chitosan nanoparticles
incorporated beauvericin (CSNp-BV) on pupal and adult
emergence
(Arvind Bharani, 2014)

25.  DNA-tagged nano gold: A new tool for the
control of the armyworm, Spodoptera litura
Fab. (Lepidoptera: Noctuidae)
 Chakravarthy et al.,
 African Journal of Biotechnology Vol. 11(38), pp. 9295-9301, 10
May, 2012

28. 4th days after treatment
5th days after treatment 6th days after treatment
3rd days after treatment

29. 7th days after treatment
control

30. THANK YOU