Microbiota intestinale e Patologie NeurodegenerativeASMaD
Presentazione a cura del Professor Giovanni Gasbarrini - XII° Congresso Nazionale FIMeG 2018 - The Silver Tsunami: l'anziano fra appropriatezza e farmaeconomia
Presentazione a cura del Professor Enrico Corazziari - M.A.S.T.E.R. ECM in Gastroenterologia: Focus on: Microbiota e dintorni - Fondazione Santa Lucia - Roma
Microbiota intestinale e Patologie NeurodegenerativeASMaD
Presentazione a cura del Professor Giovanni Gasbarrini - XII° Congresso Nazionale FIMeG 2018 - The Silver Tsunami: l'anziano fra appropriatezza e farmaeconomia
Presentazione a cura del Professor Enrico Corazziari - M.A.S.T.E.R. ECM in Gastroenterologia: Focus on: Microbiota e dintorni - Fondazione Santa Lucia - Roma
Probiotics in diarrhea in India - Special Ref to LrGG Lactobacillus Rhamnosus...Gaurav Gupta
Probiotics in diarrhea in India - Special Ref to LrGG Lactobacillus Rhamnosus GG - june 2018 unobiotic, superflora GG,
Talk taken in Chandigarh in June 2018
Dr. Tom Burkey - Host-Microbe Interactions: Effects on nutrition and physiologyJohn Blue
Host-Microbe Interactions: Effects on nutrition and physiology - Dr. Tom Burkey, University of Nebraska-Lincoln, from the 2014 Allen D. Leman Swine Conference, September 15-16, 2014, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2014-leman-swine-conference-material
Lactobacillus acidophilus CRL 1014 improved "gut health" in the SHIME(R) reactorEnrique Moreno Gonzalez
How to maintain “gut health” is a goal for scientists throughout the world. Therefore, microbiota management models for testing probiotics, prebiotics, and synbiotics have been developed.
The storyline behind the modern epidemic of inflammation driven disease goes as follows:
• Chronic degenerative conditions (Diseases of Civilization – DOCs) are on the rise and have overtaken infections as the major source of death and illness.
• This epidemiological transition is due, at least in part, to environmental changes that have increased the disease liability of genetic profiles that were previously benign.
• These DOCs are associated with increased systemic inflammation stemming from inappropriate stressor-response mechanisms.
• Much of this inflammation is generated and/or perpetuated by the gut-brain axis.
• Gut-brain axis derived inflammation leads to disruptions in psycho-neuro-immunological (PNI) balances.
• Pathophysiological mediators of PNI disturbances can be measured and treated.
• A cornerstone of said treatment is the regular consumption of a high quality multi-strain probiotic.
The carefully selected strains in Tri-Flora have been shown to:
• Decrease pro-inflammatory cytokines;
• Increase anti-inflammatory cytokine IL-10;
• Stimulate regulatory immune cells;
• Inhibit immune response to LPS (lipopolysaccharide) transduction;
• Improve intestinal barrier function;
• Enhance mucosal and systemic immune responses;
• Produce and deliver neurotransmitters such as GABA and serotonin.
And in so doing accomplish some very intriguing clinical feats:
1. Lessen symptoms of chronic fatigue syndrome;
2. Act as an adjunct to standard care in the treatment of Major Depressive Disorder;
3. Improve anxiety and reduce brain cell death; and
4. Help women lose weight.
It is for reasons such as this, that my most commonly recommended supplement for most people is a high quality probiotic. Tri-Flora by IP Formulas is my new favorite.
Increase your Understanding of the Pathogenesis of Gluten Spectrum DisordersCell Science Systems
Recently, researchers at Harvard University, Alessio Fasano et. al., and the National Institutes of Health (laboratories of immunology and cellular and molecular biology), reported real-time microscopic observations of gluten-induced neutrophil activation.
According to authors, " To what extent neutrophil function adds to, or protects against, gluten intolerance is currently under vigorous investigation."
This presentation will shed light on this question. It will also review the Fasano study and examine the role of neutrophil function in multiple disease conditions, as well as explore how neutrophil function may also play a dual role in protecting the body from the untoward effects of dietary and environmental agents.
Scientific Validation Of Polydextrose As A Fibre AndGeoffreyOsullivan
Presents an overview of the scientific evidence and methods used to prove polydextrose is a safe and effective soluble prebiotic fibre with high toleration
Probiotics in diarrhea in India - Special Ref to LrGG Lactobacillus Rhamnosus...Gaurav Gupta
Probiotics in diarrhea in India - Special Ref to LrGG Lactobacillus Rhamnosus GG - june 2018 unobiotic, superflora GG,
Talk taken in Chandigarh in June 2018
Dr. Tom Burkey - Host-Microbe Interactions: Effects on nutrition and physiologyJohn Blue
Host-Microbe Interactions: Effects on nutrition and physiology - Dr. Tom Burkey, University of Nebraska-Lincoln, from the 2014 Allen D. Leman Swine Conference, September 15-16, 2014, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2014-leman-swine-conference-material
Lactobacillus acidophilus CRL 1014 improved "gut health" in the SHIME(R) reactorEnrique Moreno Gonzalez
How to maintain “gut health” is a goal for scientists throughout the world. Therefore, microbiota management models for testing probiotics, prebiotics, and synbiotics have been developed.
The storyline behind the modern epidemic of inflammation driven disease goes as follows:
• Chronic degenerative conditions (Diseases of Civilization – DOCs) are on the rise and have overtaken infections as the major source of death and illness.
• This epidemiological transition is due, at least in part, to environmental changes that have increased the disease liability of genetic profiles that were previously benign.
• These DOCs are associated with increased systemic inflammation stemming from inappropriate stressor-response mechanisms.
• Much of this inflammation is generated and/or perpetuated by the gut-brain axis.
• Gut-brain axis derived inflammation leads to disruptions in psycho-neuro-immunological (PNI) balances.
• Pathophysiological mediators of PNI disturbances can be measured and treated.
• A cornerstone of said treatment is the regular consumption of a high quality multi-strain probiotic.
The carefully selected strains in Tri-Flora have been shown to:
• Decrease pro-inflammatory cytokines;
• Increase anti-inflammatory cytokine IL-10;
• Stimulate regulatory immune cells;
• Inhibit immune response to LPS (lipopolysaccharide) transduction;
• Improve intestinal barrier function;
• Enhance mucosal and systemic immune responses;
• Produce and deliver neurotransmitters such as GABA and serotonin.
And in so doing accomplish some very intriguing clinical feats:
1. Lessen symptoms of chronic fatigue syndrome;
2. Act as an adjunct to standard care in the treatment of Major Depressive Disorder;
3. Improve anxiety and reduce brain cell death; and
4. Help women lose weight.
It is for reasons such as this, that my most commonly recommended supplement for most people is a high quality probiotic. Tri-Flora by IP Formulas is my new favorite.
Increase your Understanding of the Pathogenesis of Gluten Spectrum DisordersCell Science Systems
Recently, researchers at Harvard University, Alessio Fasano et. al., and the National Institutes of Health (laboratories of immunology and cellular and molecular biology), reported real-time microscopic observations of gluten-induced neutrophil activation.
According to authors, " To what extent neutrophil function adds to, or protects against, gluten intolerance is currently under vigorous investigation."
This presentation will shed light on this question. It will also review the Fasano study and examine the role of neutrophil function in multiple disease conditions, as well as explore how neutrophil function may also play a dual role in protecting the body from the untoward effects of dietary and environmental agents.
Scientific Validation Of Polydextrose As A Fibre AndGeoffreyOsullivan
Presents an overview of the scientific evidence and methods used to prove polydextrose is a safe and effective soluble prebiotic fibre with high toleration
Voter Perceptions: Common Core State Standards and AssessmentsAchieve, Inc.
On February 25, 2014 Achieve hosted a webinar open to state leaders and partners in all 50 states on the release of Achieve’s third national poll – Voter Perceptions: Common Core State Standards & Tests – which shows solid majorities of voters support common standards, common assessments, and allowing teacher and students time to adjust to these new expectations. Chad Colby, Achieve’s Director of Strategic Communications and Outreach, was joined on the webinar by Alex Bratty, Partner with Public Opinion Strategies and Dave Walker, Vice President of Greenberg Quinlan Rosner Research, to present an overview of the findings and discuss implications for leaders at the state level. This year's poll shows that awareness of the Common Core State Standards (CCSS) is inching up, even though nearly two-thirds of those surveyed still have heard "nothing" or "not too much" about the CCSS. Of those that said they had read, seen or heard recently about the standards, opinions were almost equally split between favorable and unfavorable, yet a plurality still favor implementation. Once voters were read a brief description of the CCSS, a solid majority, 69%, favored implementing the standards. For the first time in Achieve's series of polls, voters were asked about the effect of the Common Core and new tests on accountability and teacher evaluations. Voters believe that both student testing and teacher evaluations are important and should continue during implementation. Consequences, voters said, should only come for teachers, students and schools after an adjustment period, with a majority favoring a one or two year adjustment period. For more information, visit http://www.achieve.org/meetings-webinars
Keynote on the adoption of mobile and the opportunities for the offshore industry. Including new hire training, recruitment and retention. The presentation sets out the trends and challenges of the new App generation, how to approach them and leverage this new generation to impact your company in a positive way. Including a poll that has been filled in online by over 60 senior crew managers, HR, and key decision makers in the offshore industry.
The IWISS Rebar Tiers are extremely fast and easy to use. Just pull the trigger and you can tie rebar perfectly in less
than a second. By using them, you can also reduce the risk of health problems such as Carpal Tunnel Syndrome. Save
time, savemoney, increase job productivity with MAX Rebar Tying Tools.
A brief info on the immunostimulants and probiotics in aquaculture. hope it will help whoever visits and go through the seminar.
Please comment if any mistakes found for my rectification as well as for others.
thank you
We are what we eat - The role of diets in the gut-microbiota-health interactionNorwich Research Park
Lecture at Summer School Nutrigenomics in Camerino Italy Sept. 2016.
The (small) intestine has increasingly been recognized to play a key role in the early phase of pro-inflammatory disturbances e.g. by enhanced overflow of dietary components to the distal intestine (ileum, colon) and affecting the gut microbiota & their metabolites (e.g. bile acids, short chain fatty acids). Transcription factors e.g. PPARγ, FXR, AHR or NRF2 are involved in host sensing mechanisms of microbial metabolites. Strong impact of dietary composition on small and large intestinal microbiota and their metabolic functions.
Targeting the (small) intestine and its microbiota with (plant) foods, bioactives, probiotics and drugs will improve gut and liver functions with strong implications for human health during life.
Patologie digestive, extradigestive e MicrobiotaASMaD
Presentazione a cura del Professor Giovanni Barbara - M.A.S.T.E.R. ECM in Gastroenterologia: Focus on: Microbiota e dintorni - Fondazione Santa Lucia - Roma
Microbiome: The genes and genomes of the microbiota, as well as the products of the microbiota and the host environment” [the collective genomes of the micro-organisms in a particular environment. Although the composition of the gut microbiota varies between individuals, the community in each individual is relatively stable over time.
Similar to Immunomodulation induced by symbiotic (20)
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
Safalta Digital marketing institute in Noida, provide complete applications that encompass a huge range of virtual advertising and marketing additives, which includes search engine optimization, virtual communication advertising, pay-per-click on marketing, content material advertising, internet analytics, and greater. These university courses are designed for students who possess a comprehensive understanding of virtual marketing strategies and attributes.Safalta Digital Marketing Institute in Noida is a first choice for young individuals or students who are looking to start their careers in the field of digital advertising. The institute gives specialized courses designed and certification.
for beginners, providing thorough training in areas such as SEO, digital communication marketing, and PPC training in Noida. After finishing the program, students receive the certifications recognised by top different universitie, setting a strong foundation for a successful career in digital marketing.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Exploiting Artificial Intelligence for Empowering Researchers and Faculty, In...Dr. Vinod Kumar Kanvaria
Exploiting Artificial Intelligence for Empowering Researchers and Faculty,
International FDP on Fundamentals of Research in Social Sciences
at Integral University, Lucknow, 06.06.2024
By Dr. Vinod Kumar Kanvaria
1. Prof Dr Ariyanto Harsono dr SpAK
CURICULUM VITAE
Tempat /tgl lahir : Kediri, 3- Juli
Agama : Islam
Status Perkawinan: Menikah
Pendidikan
S-1: Lulus Dokter : FK UNAIR, 1972
S-2: Spesialis Anak : FK UNAIR, 1982
S-3: Program Pascasarjana UNAIR, 2004
Pendidikan tambahan : Sertifikat “Fellowship on Food Allergy”,
Academisch Ziekenhuis Utrecht, Nederland, 1990-1991
Jabatan sekarang : Guru Besar Ilmu Kesehatan Anak,
Dep IKA FK UNAIR/RSU Dr.Soetomo, Surabaya.
Konsultan Alergi/Imunologi: 1992
1
3. PENDAHULUAN
• Mikrobiota pada usus manusia terdiri atas
beberapa ratus spesies, banyak diantaranya
yang sangat bermanfaat. Misalnya untuk
mencegah kolonisasi kuman patogen dan
memelihara integritas imunitas mukosa usus.
• Sebaliknya, sistem imun mukosa usus sehat
memungkinkan bertahannya mikrobiota usus
dan menghindari toleransi imunologi,
mempertahankan homeostasis usus.
3Prof DR Dr Ariyantto Harsosno SpA(K)
4. Ag
APC MHC-II Th0
IL-12/ IL-1
Th-2
Th.1
IL-1
TNF-β, IFN-γ
IL-2, IFN-γ
B-Cell
IL-4
IL-5
SEL PLASMASEL MEMORI
IL-6
IL-10
CTL
MHC-I
I L-2
IFN-γ
SEL-NK
SEL-NK AKTIF
FC-R
L
L
SEL ABNORMAL
SITOTOKSIN
SEL-LISIS
Sel Abnormal FASL
Memory Cells
ADCC
4Prof DR Dr Ariyantto Harsosno SpA(K)
Probiotic
Treg
TGFβ
5. • Prebiotik yang banyak dikenal dan digunakan
adalah oligosakarida kedelai (yang terdiri atas
rafinosa dan stakiosa), frukto-oligosakarida
(disebut juga oligofruktosa), Inulin, Laktulosa
dan Laktosukrosa. Inulin dan oligofruktosa
memiliki fungsi penting sebagai penyeimbang
fungsi gastrointestinal (menyeimbangkan
mikroflora kolon).
5Prof DR Dr Ariyantto Harsosno SpA(K)
7. Prebiotik membantu mengatasi
kerusakan barier mukosa usus
• Prebiotik
fructooligosaccharides (FOS)
dan inulin merupakan substrat
untuk fermentasi mikrobiota
usus proximal dan distal
• Fermentasi FOS dan inulin di
kolon menghasilkan short-
chain fatty acids (SCFA)¹
• SCFAs merupakan pelumas
untuk epitel ileum dan kolon,
yang membantu memelihara
integritas dinding kolon²
7Prof DR Dr Ariyantto Harsosno SpA(K)
8. Prebiotik membantu pertumbuhan flora
normal usus:
• Fermentasi FOS dan inulin
menghasilkan lactic acid4
• Lactic acid membantu
menurunkan pH kolon,
menyebabkan kolon kurang
baik untuk pertumbuhan
pathogenic bacteria2,4
• FOS dan inulin secara selektif
mempermudah pertumbuhan
bacteria yang baik dengan
dosis 8 gram per hari2-4,5
• Lactate membantu
menurunkan pH kolon untuk
mengontrol pertumbuhan
bacteria patogen
8Prof DR Dr Ariyantto Harsosno SpA(K)
10. PROBIOTIK
NON PATOGENIK
• Probiotik adalah mikroorganisme hidup yang
berasal dari tumbuhan. Mereka pada
dasarnya adalah ganggang. Bakteri probiotik
hadir di usus sejak manusia lahir. Bakteri ini
terus menerus mengalahkan mikroba lain
dalam tubuh, dan hal ini dapat dapat
mengancam populasinya. Selain itu,
pengobatan antibiotik dapat membunuh
semua bakteri dalam tubuh, termasuk
bakteri probiotik. 10Prof DR Dr Ariyantto Harsosno SpA(K)
14. Bagaimana bacteria intra luminal
membangkitkan respons imun sistemik?:
• (A) Fluoresensi di Peyer's
patches usus halus
• (B) Fluoresensi di lamina
propria usus halus
• (C) Fluoresensi di nodule
dan crypta usus besar
14Prof DR Dr Ariyantto Harsosno SpA(K)
Internalisasi bacteria kedalam GI
15. Sebagian mikrobiota difagosit dan dimusnahakan. Sebagian epitopnya dibantu dengan IL6
menimbulkan clonal IgA B lymphocytes, meningkatkan jumlah IgA-producing cells dan transfer ke
plasma sel usus. IL-6 bersama dengan IL-4 dan TGF-ß dapat menginduksi T-independent switch dari IgM
ke IgA dipermukaan sel B.
(EC, intestinal epithelial cells; MQ, macrophages; TL, T lymphocytes; BL, B lymphocytes; MS, mast cells;
PC, plasma cells).
15Prof DR Dr Ariyantto Harsosno SpA(K)
22. Cara kerja antibodi yang di-induksi
Probiotik
22Prof DR Dr Ariyantto Harsosno SpA(K)
23. Orihara et. al. WAO Journal 2008, 9-14
The profiles of immune
response in healthy subject
23Prof DR Dr Ariyantto Harsosno SpA(K)
24. The
balancing
square
model.
The 4 T-cell
types (Th1,
Th2, Th17,
and Treg)
antagonize
each other.
Proinflammatory T-cell
subsets, that is, Th17 cells and
Th2 cells that are capable of
producing high levels of TNF-
> (iTh2 cells) are upregulated
Th2 cells that are capable of
producing IL-10 (rTh2 cells)
may be up-regulated, and
Th17 cells may be inactivated
Orihara et. al. WAO Journal 2008, 9-14
The profiles of immune response in
symptomatic & asymptomatic atopy
24Prof DR Dr Ariyantto Harsosno SpA(K)
26. WHAT ARE IMMUNOMODULATION INDUCED BY PROBIOTICS?
Organism days No. of cells producingb
:
Cytokines
IgA
TNF- a IFN- g IL-2 IL-12 IL-4 IL-10
L. casei
CRL 431
2 90 ± 8* 124 ± 15* 24 ± 6 13 ± 3 86 ± 23* 40 ± 2* 99 ± 21
L.
delbrueckii
subsp.
bulgaricus
CRL 423
2 79 ± 6* 59 ± 22* 40 ± 8 23 ± 11 67 ± 4* 85 ± 9* 135 ± 23*
L.
acidophilu
s CRL 724
7 Strains
2
2
52 ± 7
98+/-8
51 ± 25*
165+/-34
27 ± 8
50+/-23
30 ± 16*
45+/-60
44 ± 11
30+/-46
30 ± 7
90+/-54
173 ± 24*
199+/-76
Control 2 24 ± 4 17 ± 6 31 ± 12 11 ± 2 27 ± 7 18 ± 6 98 ± 17
26Prof DR Dr Ariyantto Harsosno SpA(K)
27. Probiotic modulation of humoral
immunity
Bifido
bacterium
bifidus, (live)
Human Total IgA and
response to
polio virus
Increased
sIgA
27Prof DR Dr Ariyantto Harsosno SpA(K)
28. Effects of probiotics on nonspecific
immunity
Probacteria Species Assessment Effect
Lactobacillus casei
Shirota, intravenous
Rodent Peritoneal macrophages Increased phagocytosis
L. acidophilus or
Bifidobacterium bifidum,
oral (live)
Rodent Peritoneal or peripheral
blood macrophages
Enhanced phagocytosis
L. acidophilus or casei,
oral (live)
Rodent Resident peritoneal
macrophages
Enhanced phagocytosis
L. casei Shirota
7 strains oral (live)
Human
Peripheral blood No effect on natural killer
cell cytolysis in vitro
Enhanced phagocytosis
28Prof DR Dr Ariyantto Harsosno SpA(K)
29. Effect of probiotics on select human
disease1
Disease Probiotic Assessment Effect
Asthma Lactobacillus
acidophilus, oral
(live)
Serum IgE and IL-4
Lymphocyte
proliferation
No alterations
Rotavirus infection 7 starins, oral (live) Diarrhea Serum
total IgA and IgM
and anti-rotavirus
IgM and IgG
Duration of
diarrhea decreased;
Increased anti-
rotavirus IgA
Atopic Dermatitis L. casei, oral (live) Anti-ß-lactoglobulin
IgA- secreting cells
Number increased
Clinical
improvement 29Prof DR Dr Ariyantto Harsosno SpA(K)
30. Synbiotic dalam pencegahan
Asthma
• Bayi dengan Dermatitis Atopika lebih sering
menderita Asthma.
• Pemberian Synbiotic pada bayi-bayi
Dermatitis Atopika mencegah terjadinya
Asthma pada masa naka-anak.
(van der Aa LB, Synbiotics prevent asthma-like symptoms in infants with
atopic dermatitis, Allergy, 2011:68:170-7)
30Prof DR Dr Ariyantto Harsosno SpA(K)
31. Regulator Treg
• Probiotic untuk
penderita alergi,
Inflammatory Bowel
Disease, auto imun
membangkitkan
keseimbangan kearah
Treg melalui resptor TLR2,
TLR4, TLR6.
(Rook GAW: microbes,
immunoregulation, and
Gut.accessed fromProf DR Dr Ariyantto Harsosno SpA(K) 31
PROBIOTIC
IgA
32. Menurunkan kejadian penyakit
alergi
• Probiotic Menyebabkan Keseimbangan
intestinal dan sistem imun menurunkan
kejadian penyakit alergi.
(Hormannsperger G: Gut matters: microbe-host interactions in allergic
disease. J Allergy clin Immunol 2012;129:1452-9)
32Prof DR Dr Ariyantto Harsosno SpA(K)
33. Meningkatkan CD4 T limfosit
• Probiotic merangsang
klonal CD4 T limfosit
berperan dalam
perbaikan penyakit
alergi.
(Mazmanian SK: The love–hate
relationship between bacterial
polysaccharides and the host
immune system. Nature Reviews
Immunology, 2006: 6, 849-858)
Prof DR Dr Ariyantto Harsosno SpA(K) 33
38. TH1<Treg <TH2
TH1 > Treg > TH2
TH1, Treg and TH2 immunoregulation through TLR2 and TLR4 by
Lactobacillus plantarum IS-10506 probiotics in reducing allergic
reaction. Experimental Study in Mice Subjects
Allergic
Reaction
Adaptive
Immunity
Innate
Immunity
Control group LIS probiotics group
(Endaryanto A, et.al., 2006)Study on animal model
38Prof DR Dr Ariyantto Harsosno SpA(K)
Schematic representation of the multiple consequences of the cross-talk between the probiotic bacteria and the intestinal mucosa. At the intestinal epithelial level, probiotic bacteria may allow beneficial effects through transient colonization and/or release of bioactive compounds. This translates into reinforcement of the intestinal barrier as well as direct modulation of epithelial cell functions including cytokine and chemokine release. Although a limited event, translocation of bacteria to the lamina propria may affect innate and adaptive immunity by activating production of cytokines by monocytes/macrophages. Sampling by M cells in Peyer's patches ( PP ) and subsequent engulfment by dendritic cells ( DC ) of the innate immune system may contribute to present microbial antigens to naïve T cells in the PP and mesenteric lymph nodes ( MLN ). This allows IgA antibody-mediated mucosal response to take place against the bacterium to prevent overgrowth and spreading beyond MLN but also, for example, to the antigen coded by a recombinant probiotic strain used as a vaccine. Remarkably, the same processing pathway plays a critical role in the shaping of the mucosal immune system toward a noninflammatory, tolerogenic pattern that takes place through the induction of regulatory T cells. Author's caution: The scheme is a simplified synthesis obtained from data collected in vivo and in vitro in various experimental models; the specific effects of a particular probiotic on the development of local and systemic responses must be considered on a case-by-case basis.
