This document discusses medical applications of blood group systems. It covers blood transfusions, identification of disease susceptibility, blood compatibility, blood incompatibility, blood banking, whole blood and blood components, transfusion reactions, and clinical implications such as paternity disputes and criminal cases. It provides details on various anticoagulant solutions, storage of whole blood and components, and separation of components through centrifugation. It also summarizes immediate and delayed transfusion reactions.
The Compatibility can be determined by matching the different blood group systems, such as ABO and Rh system, and/or by directly testing for the presence of antibodies against a sample of donor tissues or blood.
The main purpose of this test is to distinguish the appearance of antibodies in the recipient against the red blood cells of the donor. These antibodies can be found on the surface of red blood cells of the donor after transfusion.
how to select a healthy donor & care of donor .A healthy donor is one of the most vital part of transfusion medicine for safe transfusion of blood & blood product
The Compatibility can be determined by matching the different blood group systems, such as ABO and Rh system, and/or by directly testing for the presence of antibodies against a sample of donor tissues or blood.
The main purpose of this test is to distinguish the appearance of antibodies in the recipient against the red blood cells of the donor. These antibodies can be found on the surface of red blood cells of the donor after transfusion.
how to select a healthy donor & care of donor .A healthy donor is one of the most vital part of transfusion medicine for safe transfusion of blood & blood product
blood and blood component have an important role in transfusion medicine. when blood contain all its part and no separation is done thats known as whole blood but when you centrifuge and separate it that is know as component. transfusion of whole blood is now adays absolute from transfusion service and blood components are transfuses now a days which is a good practice and beneficial for the patient
blood and blood component have an important role in transfusion medicine. when blood contain all its part and no separation is done thats known as whole blood but when you centrifuge and separate it that is know as component. transfusion of whole blood is now adays absolute from transfusion service and blood components are transfuses now a days which is a good practice and beneficial for the patient
It contains indications of blood and blood products and perioperative blood therapy that we usually follow in Aiims Patna ..its is most recent one made in April 2020
Components Of Blood (For Transfusion)
• Each unit of blood is tested for evidence of hepatitis-b,
hepatitis-c, Human Immune deficiency Virus I & II.
• The blood is then processed into sub-components.
• Whole blood
• Packed cell volume
• Fresh frozen plasma
• Platelets
• Cryoprecipitate
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
4. BLOOD COMPATIBILITY
Impossible to obtain perfectly matched blood for transfusion.
Routinely used system- ABO& Rh.
Other antigens are too weakto be of importance.
Choice of donor:
1. Recipient’s plasma should not contain antibodies against donor’s RBCs
2. Donor’s plasma should not contain antibody that will damage the
recipient’s red cells.
3. Donor’s red cells should not have any antigen lacking in the recipient.
8. NEEDOF BLOODBANK-
• STORE VIABLE AND FUNCTIONAL BLOOD AS WELL AS BLOOD COMPONENTS
• PREVENT PHYSICAL CHANGES IN BLOOD & BLOOD COMPONENTS
• MINIMISE CONTAMINATION IN BLOOD & BLOOD COMPONENTS
9. ANTICOAGULANT SOLUTIONS-
• ACD- ACIDCITRATE DEXTROSE
TEMPERATURE- 2-6°C FOR 21 DAYS.
CITRATE - ANTICOAGULANT BY CHELATING CALCIUM.
DEXTROSE – INCREASES POST-TRANSFUSION SURVIVAL OF RED CELLS.
CITRIC ACID – PREVENTS CARAMELIZATION OF GLUCOSE DURING AUTOCLAVE.
10. • CPD– CITRATE PHOSPHATE DEXTROSE
PHOSPHATE CAN INCREASE THE POST-TRANSFUSION SURVIVAL RATE OF RBC.
• HEPARIN – PREVENTS COAGULATION BY INACTIVATING THROMBIN AFTER
COMPLEXING WITH ANTI-THROMBIN III AND THROMBIN.
• EDTA – ACTS AS CHELATING AGENT AND BINDS CALCIUM IONS.
11. WHOLE BLOOD & BLOOD
COMPONENTS:
• Whole blood is one unit of donor blood which is collected in a suitable anticoagulant-
preservative solution.
• Blood components are constituents separated from whole blood by differential
centrifugation or by apheresis.
12.
13. WHOLE BLOOD
• Total volume = 400 ml (350 ml of blood + 49 ml of anticoagulant).
• Storage temperature = 2-6°C (in refrigerator)
• Shelf life = 35 days (in CPDA-1).
14. BLOOD COMPONENTS
• Separation of blood components is achieved using centrifugation.
• Separation is carried out in double or triple bags with closed internal
tubings.
• Blood should be processed for component within 6 hours of collection.
15.
16.
17.
18.
19. PACKED RED CELLS
• Prepared by removing most of the plasma from one unit of whole blood.
• It is done by either allowing whole blood to sediment overnight in a refrigerator at 2-6°C or by
spinning whole blood in a refrigerated centrifuge.
• Supernatant plasma is separated from red cells in a closed system.
• Red cells and small amount of plasma are left behind in the primary blood bag.
20. RED CELLS IN ADDITIVE SOLUTION-
• Commonly used additive solution contains Saline,Adenine, Glucose & Mannitol.
• Whole Blood is collected in primary collection bag which contained CPDA-1 and centrifuged.
• Maximum amount of plasma is removed and transferred to one satellite bag.
• Additive solution from second satellite bag is then transferred into the primary collection bag.
21. LEUKOCYTE-POOR RED CELLS-
• Obtained by passing blood through a special leukocyte-depletion filter.
• Should contain less than 5 X 106
white cells per bag.
• Cannot prevent graft-versus-host disease.
22. IRRADIATED RED CELLS-
• Gamma irradiation inhibits donor lymphocyte replication.
• Gamma-irradiated red cells can prevent graft-versus-host
disease in susceptible individuals.
23. WASHED RED CELLS-
• Packed red cells are washed with normal saline to
remove plasma proteins,WBCs and platelets.
24. FROZEN RED CELLS-
• Frozen red blood cells can be stored for up to 10 years.
• Donor red cells with rare blood groups can be stored frozen.
• Cryoprotective agent such as glycerol is added during freezing and thawing.
• Virtually free from lymphocytes, platelets and plasma.
25. PLATELETS-
• It can be obtained by differential centrifugation of one unit of whole
blood or by Plateletpheresis.
• Donor pheresis is a procedure in which the donor is connected to an
automated cell separator machine which withdraws whole blood,
retains desired component and returns back the remainder of the
blood to the donor.
26. PLATELET CONCENTRATE-
• Obtained by differential centrifugation of one unit of whole blood within 6 hours of donation
and before refrigeration.
• One unit of whole blood is centrifuged at low speed to obtain platelet-rich plasma or PRP.
• PRP is then transferred to attached satellite bag and centrifuged at high speed.
• Platelet aggregates at the bottom and platelet-poor plasma (PPP) remains at the top.
• Most of the PPP is returned to the primary collection bag.
27. PLATELET CONCENTRATE-
• Transfusion of one unit of platelet concentrate will raise the platelet count in the recipient by about
5000/ microlitres.
• Usual adult dose is 4 to 6 units or 1 unit/10 kg of body weight.
• These units are collected from different donors and are pooled into one bag before transfusion.
• It is preferable to transfuse platelet concentrate of same or compatible ABO group and similar Rh
group.
• Platelet concentrate can be stored at 20 to 24°C with continuous agitation for 3 to 5 days.
28. PLATELETPHERESIS-
• The donor is connected to blood cell separator machine through which whole blood is
collected in an anticoagulant solution.
• Then, platelets are separated and retained.
• The remaining blood components are returned back to the donor.
• Large number of platelets can be obtained from a single donor.
29. GRANULOCYTE CONCENTRATE-
• Rarely used.
• It can be obtained by leukapheresis or by differential centrifugation.
• Leukapheresis is preferred.
• Administration of Corticosteroids to the donor may enhance leukapheresis by providing
more granulocytes.
31. FRESH FROZEN PLASMA-
• Plasma is separated and transferred to the attached satellite bag.
• Rapidly frozen at -25°C or lower temperature.
• Must be performed within 6 hours of collection.
• Can be stored for 1 year at temperature below -25 ° C.
• When required for transfusion, it is thawed between 30 and 37 ° C and then stored in blood
bank refrigerator at 2 to 6°C.
• Should be transfused within 2 hours of thawing.
32. CRYOPRECIPITATE-
• Prepared by slowly thawing one unit of FFP at 4 to 6°C.
• Plasma and a white precipitate are obtained.
• After centrifugation, most of the supernatant plasma is removed leaving behind sediment of
cryoprecipitate which is suspended in 10 to 20 ml of plasma.
• The unit is then refrozen at -25°C or colder temperature.
• It can be stored for 1 year at this temperature.
• When required for transfusion, it is thawed between 30 and 37°C and then stored in blood bank
refrigerator at 2 to 6°C till transfusion.
37. Thank YouThank You
Presented by- Bishwarup S.
References-
1. Kuby immunology/ 6th ed. / Thomas J. Kindt,
Richard A. Goldsby, Barbara A. Osborne.
2. Banaji N. Ananthanarayan and Paniker's Textbook of
Microbiology/ 10th
ed./ Indian J Med Microbiol
2013;31:423.
3. Saran RK. Transfusion medicine technical manual.
DGHS/ 2nd ed./ India: Ministry of Health and Family
Welfare; 2003.