High performance liquid chromatography
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Introduction to Chromatography, History, Working Principle and Its types. Introduction to High Performance Liquid Chromatography,Its Working parts and Applications
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High Pressure Liquid Chromatography (HPLC)
The document discusses high-performance liquid chromatography (HPLC), including its basic principles, types, instrumentation, and applications. HPLC forces solvents through columns under high pressure to separate sample components faster than traditional chromatography. There are four main types depending on the stationary phase used: normal phase uses polar stationary and nonpolar mobile phases; reverse phase uses nonpolar stationary and polar mobile; size-exclusion separates by molecular size; ion-exchange uses charged stationary phases. HPLC instrumentation includes pumps, injectors, columns, detectors, and data collection systems. Key applications are in manufacturing quality control, environmental monitoring, forensics, food analysis, research, and medical analysis of substances in blood, urine, and tissues.
Chromatography
This document provides an overview of chromatography techniques. It discusses the definition and history of chromatography, and describes several types including paper chromatography, thin layer chromatography, column chromatography, gas chromatography, and high performance liquid chromatography. Specific details are provided on the principles, procedures, applications, advantages and disadvantages of paper chromatography and thin layer chromatography. Key differences between these two techniques are also compared.
Chromatography
Chromatography is a method of separating components of a mixture through their interactions with two phases - a stationary phase and a mobile phase. The components are distributed between the phases based on properties like solubility and affinity. There are several types of chromatography classified by the shape of the stationary phase (e.g. thin layer), the state of the mobile phase (e.g. gas, liquid), or the interaction between solute and stationary phase (e.g. adsorption, partition). Chromatography techniques are used in various applications including pharmaceutical quality control, forensic analysis, and biological research like protein purification.
Hplc
This document provides an overview of high-performance liquid chromatography (HPLC). It describes HPLC as a chromatographic technique used to separate components of a mixture for identifying, quantifying, or purifying individual components. The document outlines the history, instrumentation, operation, types, advantages, and disadvantages of HPLC. It explains that HPLC involves a mobile phase, column, pump, injector, detector, and computer to separate sample components based on their interactions with the stationary phase.
Planar Chromatography
The document describes planar chromatography techniques, specifically thin layer chromatography (TLC). It explains that TLC separates mixtures by using a thin stationary phase like silica gel coated on a plate and a mobile phase liquid solvent. The steps of TLC are described as sample application, development where separation occurs, visualization under UV light, and interpretation by calculating Rf values. Applications for separating lipids, carbohydrates and other compounds are outlined.
High performance liquid chromatography
HPLC is a technique used to separate components in a mixture using pumps to pass a pressurized liquid solvent containing the sample through a column filled with an adsorbent material. Each component interacts slightly differently with the material, causing different flow rates and separation. HPLC has applications in manufacturing like pharmaceutical production, legal purposes like drug testing, research like separating biological samples, and medical uses like detecting vitamin levels. It provides superior resolving power over traditional chromatography to distinguish between compounds.
Column chromatography
A chromatographic technique used for the separation and purification of solids & liquids
principle is based on Adsorption
High performance liquid chromatography (HPLC)
High performance liquid chromatography (HPLC) is a separation technique used to separate mixtures. It uses columns packed with small particle sizes under high pressure, allowing better separation than traditional liquid chromatography. HPLC involves pumping a mobile phase through a column containing a stationary phase, separating components as they flow through at different rates based on interactions with the phases. Components are then detected and quantified as they exit the column. HPLC provides rapid, sensitive, and precise separation of mixtures and is widely used in fields like pharmaceuticals, chemistry, and environmental analysis.
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High Pressure Liquid Chromatography (HPLC)
The document discusses high-performance liquid chromatography (HPLC), including its basic principles, types, instrumentation, and applications. HPLC forces solvents through columns under high pressure to separate sample components faster than traditional chromatography. There are four main types depending on the stationary phase used: normal phase uses polar stationary and nonpolar mobile phases; reverse phase uses nonpolar stationary and polar mobile; size-exclusion separates by molecular size; ion-exchange uses charged stationary phases. HPLC instrumentation includes pumps, injectors, columns, detectors, and data collection systems. Key applications are in manufacturing quality control, environmental monitoring, forensics, food analysis, research, and medical analysis of substances in blood, urine, and tissues.
Chromatography
This document provides an overview of chromatography techniques. It discusses the definition and history of chromatography, and describes several types including paper chromatography, thin layer chromatography, column chromatography, gas chromatography, and high performance liquid chromatography. Specific details are provided on the principles, procedures, applications, advantages and disadvantages of paper chromatography and thin layer chromatography. Key differences between these two techniques are also compared.
Chromatography
Chromatography is a method of separating components of a mixture through their interactions with two phases - a stationary phase and a mobile phase. The components are distributed between the phases based on properties like solubility and affinity. There are several types of chromatography classified by the shape of the stationary phase (e.g. thin layer), the state of the mobile phase (e.g. gas, liquid), or the interaction between solute and stationary phase (e.g. adsorption, partition). Chromatography techniques are used in various applications including pharmaceutical quality control, forensic analysis, and biological research like protein purification.
Hplc
This document provides an overview of high-performance liquid chromatography (HPLC). It describes HPLC as a chromatographic technique used to separate components of a mixture for identifying, quantifying, or purifying individual components. The document outlines the history, instrumentation, operation, types, advantages, and disadvantages of HPLC. It explains that HPLC involves a mobile phase, column, pump, injector, detector, and computer to separate sample components based on their interactions with the stationary phase.
Planar Chromatography
The document describes planar chromatography techniques, specifically thin layer chromatography (TLC). It explains that TLC separates mixtures by using a thin stationary phase like silica gel coated on a plate and a mobile phase liquid solvent. The steps of TLC are described as sample application, development where separation occurs, visualization under UV light, and interpretation by calculating Rf values. Applications for separating lipids, carbohydrates and other compounds are outlined.
High performance liquid chromatography
HPLC is a technique used to separate components in a mixture using pumps to pass a pressurized liquid solvent containing the sample through a column filled with an adsorbent material. Each component interacts slightly differently with the material, causing different flow rates and separation. HPLC has applications in manufacturing like pharmaceutical production, legal purposes like drug testing, research like separating biological samples, and medical uses like detecting vitamin levels. It provides superior resolving power over traditional chromatography to distinguish between compounds.
Column chromatography
A chromatographic technique used for the separation and purification of solids & liquids
principle is based on Adsorption
High performance liquid chromatography (HPLC)
High performance liquid chromatography (HPLC) is a separation technique used to separate mixtures. It uses columns packed with small particle sizes under high pressure, allowing better separation than traditional liquid chromatography. HPLC involves pumping a mobile phase through a column containing a stationary phase, separating components as they flow through at different rates based on interactions with the phases. Components are then detected and quantified as they exit the column. HPLC provides rapid, sensitive, and precise separation of mixtures and is widely used in fields like pharmaceuticals, chemistry, and environmental analysis.
Chromatography and Its Types
Chromatography is a technique used to separate and identify components of a mixture. It works by distributing molecules between a stationary and mobile phase. Molecules that spend more time in the mobile phase move faster through the column. There are several types of chromatography classified by mobile phase or separation mechanism, including gas chromatography which uses gases, thin layer chromatography which uses adsorbents on plates, and liquid chromatography which uses liquids. Chromatography is used in various applications such as pharmaceutical analysis, environmental monitoring, and forensic analysis.
High Performance Thin Layer Chromatography(HPTLC)
High performance thin layer chromatography its principle ,difference between HPTLC and TLC,components involved ,and application of hptlc
High performance liquid chromatography (HPLC)
High performance liquid chromatography (HPLC) head points:
HPLC Advantages Vs GC
Instrumentation
HPLC System
Separations
Mobile Phase Reservoirs
Degasser
Aim of Gradient system
High/Low pressure gradient system
HPLC Pump Criteria
HPLC Pumps: Types
Reciprocating Pumps
Sample introduction
Manual Injector
Auto Injector
HPLC Modes
The Mobile Phase
Hydrophobic interaction
Common reverse phase solvents
Detectors
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Dark field microscope
Dark field microscopy produces bright images of unstained samples against a dark background. It works by using a condenser with an opaque disk to block light entering the objective lens directly, allowing only light reflected off the sample to pass through. This causes specimens to appear bright on a dark background. It is useful for viewing transparent or unstained samples like bacteria, cells, and minerals due to the contrast it provides.
HPLC AND ITS APPLICATIONS
This document provides an overview of high performance liquid chromatography (HPLC). It discusses the basic principles of chromatography and how HPLC works to separate compounds. HPLC uses a stationary phase and mobile phase to separate samples based on properties like polarity. Different separation modes are used like normal phase, reversed phase, ion exchange, size exclusion, and affinity chromatography. Instrumentation includes the column, detector, pump, injection port, and auto-injector. Various detectors can be used like UV/Vis detectors and photo diode array detectors. HPLC provides high resolution, sensitivity, repeatability and is useful for analyzing small samples and purifying compounds.
thin layer chromatography
Thin layer chromatography (TLC) is a technique used to separate mixtures of compounds and identify their components. It involves spotting a sample onto a thin layer of adsorbent material and using a mobile phase solvent to migrate the components at different rates based on their interactions with the stationary and mobile phases. TLC is useful for identifying unknown compounds, analyzing purity, and separating mixtures. It has advantages over column chromatography like being faster, using less solvent, and allowing detection of both colored and non-colored compounds.
HPLC - High Performance Liquid Chromatography
The document discusses High Performance Liquid Chromatography (HPLC). It explains that HPLC is a type of liquid chromatography that uses pumps to force the mobile phase through a column packed with porous particles or beads under high pressure. This allows for effective separation of mixtures as the components elute from the column at different rates depending on their interactions with the stationary phase. The document provides details on the typical components of an HPLC system including the solvent delivery system, pumps, injector, columns, detectors, and data processing unit.
HPLC-High Performance Liquid Chromatography
HPLC, or high-performance liquid chromatography, is an analytical technique used to separate, identify, and quantify components in a mixture. It works by pumping a pressurized mobile liquid phase through a column containing a stationary phase, which causes the components in a sample to separate as they migrate through the column at different rates. Common components of an HPLC system include the pump, injector, column, detector, and recorder or computer system. HPLC has advantages of speed, efficiency, accuracy, and versatility in chemical analysis. It is used in various applications such as drug analysis, environmental analysis, and industrial quality control.
High Performance Liquid chromatography (HPLC)
The document provides an overview of high performance liquid chromatography (HPLC). It describes key components of HPLC systems including pumps to deliver solvent at stable flow rates, columns for molecular separation, and detectors for recognizing analytes. The separation principle is based on the distribution of analytes between a mobile liquid phase and stationary column packing material. Different constituents are eluted at different times, achieving separation. HPLC is widely used in pharmaceutical applications such as drug development, production quality control, and stability testing.
Isoelectric focusing
This document discusses isoelectric focusing, a technique used to separate proteins based on their isoelectric point (PI). Proteins are subjected to an electric field within a pH gradient, which causes them to migrate to the point in the gradient where their net charge is zero (their PI). Different proteins have different PIs and will therefore migrate to distinct positions in the gel. Isoelectric focusing provides high resolution separation and is useful for research applications such as taxonomy, cytology and immunology.
fluroscence spectroscopy
Fluorescence spectroscopy involves using ultraviolet light to excite electrons in molecules, causing them to emit visible light. The emitted light has a longer wavelength than the absorbed light. Fluorimeters are used to measure fluorescence, exciting samples at an absorption wavelength and measuring emission at a longer fluorescence wavelength. Fluorescence spectroscopy is useful for applications like determining fluorescent drugs in formulations, carrying out limit tests for fluorescent impurities, and studying drug-protein binding in bioanalysis.
Chromatography
Chromatography is a technique used to separate and identify the components of a mixture. It works by allowing the molecules present in the mixture to distribute themselves between a stationary and a mobile medium. Molecules that spend most of their time in the mobile phase are carried along faster. There are different types of chromatography classified according to the mobile phase used and the packing of the stationary phase. Chromatography techniques include thin layer chromatography, paper chromatography, column chromatography, gas chromatography and liquid chromatography. These techniques find application in various fields to analyze sample mixtures.
Thin layer Chromatography (TLC)
Thin layer chromatography is a technique used to separate mixtures into their components. It involves a stationary phase, such as silica gel, and a mobile phase, such as a solvent. A sample is applied to the plate and the mobile phase moves up the plate, separating the components by their interaction with the stationary phase. The separated components are then detected and quantified. TLC is useful for analyzing mixtures and is simpler and less expensive than other chromatography methods.
Classification of Chromatography
This document provides information about chromatography. It defines chromatography as a method of separation where components are distributed between a stationary and mobile phase. The stationary phase can be solid or liquid, and the mobile phase can be liquid, gas, or supercritical fluid. Various types of chromatography are described based on the interaction between components and phases, including thin layer chromatography, column chromatography, gas chromatography, and liquid chromatography. Key applications and principles of different chromatographic techniques are also summarized.
chromatographic techniques
Chromatography is a technique used to separate and identify the components of a mixture. It works by allowing molecules to distribute themselves between a stationary and mobile phase, so that molecules that interact more with the mobile phase move faster. Chromatographic techniques can be classified based on the interaction with the stationary phase or physical state of the mobile phase. Key techniques include adsorption, partition, ion exchange, exclusion, gas, liquid, and thin layer chromatography. Proper sample preparation and development conditions are important for achieving optimal separation and resolution of components in the mixture.
Classification of chromatography
Chromatography is a method of separating mixtures into individual components using a stationary and mobile phase. There are several types depending on the physical state of the phases and interaction between the phases and components. Liquid chromatography uses a liquid mobile phase passing through a solid or liquid stationary phase to separate components. Gas chromatography uses a gas mobile phase to separate volatile components. Size exclusion and ion exchange chromatography separate based on molecular size or charge.
chromatography
Chromatography is a technique used to separate mixtures by distributing components between two phases - a stationary phase and a mobile phase. Mikhail Tswett discovered chromatography in 1906 when separating plant pigments. There are various types of chromatography classified by mobile phase (liquid, gas), stationary phase material (thin layer, paper, column), or separation mechanism (adsorption, partition, ion exchange, size exclusion, affinity). Chromatography has many applications in science and industry, including purification of antibiotics, vaccines, enzymes, and other biomolecules.
Paper Chromatography
Chromatography is a technique used to separate mixtures into individual components. It works by using the differential affinity and solubility of components for both a mobile phase that carries the mixture and a stationary phase that the mixture passes through. The separation of components depends on how strongly they interact with each phase. Common types of chromatography include liquid chromatography, gas chromatography, paper chromatography, and thin-layer chromatography. Paper chromatography can be used to separate the dyes in markers by running samples along a strip with an increasing solvent mixture as the mobile phase.
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
This document provides an introduction to high performance liquid chromatography (HPLC). It discusses the basic components and principles of HPLC, including the stationary and mobile phases, pumps to move the mobile phase through the column at high pressure, and various detectors used to analyze the separated components as they elute from the column. It also describes the different types of HPLC based on the mode of separation, elution technique, scale of operation, and type of analysis performed.
Size exclusion chromatograpy
Size exclusion chromatography, also known as gel filtration chromatography, separates molecules based on their size and molecular weight. Larger molecules pass through the porous beads of the stationary phase more quickly than smaller molecules that can enter the pores. This document discusses the basic principles, history, applications, and factors affecting size exclusion chromatography such as column length and packing. It is commonly used to purify proteins and other biomolecules.
Hplc by Bachaspati jana
This document discusses high performance liquid chromatography (HPLC). It begins by defining chromatography as a technique used to separate mixtures into their individual components using both a stationary and mobile phase. It then describes some key aspects of HPLC, including that it uses high pressure to force the mobile phase through a column with small particle sizes for better separation. The document outlines the basic components of an HPLC system, including the pump, injector, column, detectors, and computer. It also discusses some common terms and uses for HPLC, such as separating and analyzing compounds in research, quality control, and environmental monitoring.
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Chromatography and Its Types
Chromatography is a technique used to separate and identify components of a mixture. It works by distributing molecules between a stationary and mobile phase. Molecules that spend more time in the mobile phase move faster through the column. There are several types of chromatography classified by mobile phase or separation mechanism, including gas chromatography which uses gases, thin layer chromatography which uses adsorbents on plates, and liquid chromatography which uses liquids. Chromatography is used in various applications such as pharmaceutical analysis, environmental monitoring, and forensic analysis.
High Performance Thin Layer Chromatography(HPTLC)
High performance thin layer chromatography its principle ,difference between HPTLC and TLC,components involved ,and application of hptlc
High performance liquid chromatography (HPLC)
High performance liquid chromatography (HPLC) head points:
HPLC Advantages Vs GC
Instrumentation
HPLC System
Separations
Mobile Phase Reservoirs
Degasser
Aim of Gradient system
High/Low pressure gradient system
HPLC Pump Criteria
HPLC Pumps: Types
Reciprocating Pumps
Sample introduction
Manual Injector
Auto Injector
HPLC Modes
The Mobile Phase
Hydrophobic interaction
Common reverse phase solvents
Detectors
https://www.linkedin.com/in/preeti-choudhary-266414182/
https://www.instagram.com/chaudharypreeti1997/
https://www.facebook.com/profile.php?id=100013419194533
https://twitter.com/preetic27018281
Please like, share, comment and follow.
stay connected
If any query then contact:
chaudharypreeti1997@gmail.com
Thanking-You
Preeti Choudhary
Dark field microscope
Dark field microscopy produces bright images of unstained samples against a dark background. It works by using a condenser with an opaque disk to block light entering the objective lens directly, allowing only light reflected off the sample to pass through. This causes specimens to appear bright on a dark background. It is useful for viewing transparent or unstained samples like bacteria, cells, and minerals due to the contrast it provides.
HPLC AND ITS APPLICATIONS
This document provides an overview of high performance liquid chromatography (HPLC). It discusses the basic principles of chromatography and how HPLC works to separate compounds. HPLC uses a stationary phase and mobile phase to separate samples based on properties like polarity. Different separation modes are used like normal phase, reversed phase, ion exchange, size exclusion, and affinity chromatography. Instrumentation includes the column, detector, pump, injection port, and auto-injector. Various detectors can be used like UV/Vis detectors and photo diode array detectors. HPLC provides high resolution, sensitivity, repeatability and is useful for analyzing small samples and purifying compounds.
thin layer chromatography
Thin layer chromatography (TLC) is a technique used to separate mixtures of compounds and identify their components. It involves spotting a sample onto a thin layer of adsorbent material and using a mobile phase solvent to migrate the components at different rates based on their interactions with the stationary and mobile phases. TLC is useful for identifying unknown compounds, analyzing purity, and separating mixtures. It has advantages over column chromatography like being faster, using less solvent, and allowing detection of both colored and non-colored compounds.
HPLC - High Performance Liquid Chromatography
The document discusses High Performance Liquid Chromatography (HPLC). It explains that HPLC is a type of liquid chromatography that uses pumps to force the mobile phase through a column packed with porous particles or beads under high pressure. This allows for effective separation of mixtures as the components elute from the column at different rates depending on their interactions with the stationary phase. The document provides details on the typical components of an HPLC system including the solvent delivery system, pumps, injector, columns, detectors, and data processing unit.
HPLC-High Performance Liquid Chromatography
HPLC, or high-performance liquid chromatography, is an analytical technique used to separate, identify, and quantify components in a mixture. It works by pumping a pressurized mobile liquid phase through a column containing a stationary phase, which causes the components in a sample to separate as they migrate through the column at different rates. Common components of an HPLC system include the pump, injector, column, detector, and recorder or computer system. HPLC has advantages of speed, efficiency, accuracy, and versatility in chemical analysis. It is used in various applications such as drug analysis, environmental analysis, and industrial quality control.
High Performance Liquid chromatography (HPLC)
The document provides an overview of high performance liquid chromatography (HPLC). It describes key components of HPLC systems including pumps to deliver solvent at stable flow rates, columns for molecular separation, and detectors for recognizing analytes. The separation principle is based on the distribution of analytes between a mobile liquid phase and stationary column packing material. Different constituents are eluted at different times, achieving separation. HPLC is widely used in pharmaceutical applications such as drug development, production quality control, and stability testing.
Isoelectric focusing
This document discusses isoelectric focusing, a technique used to separate proteins based on their isoelectric point (PI). Proteins are subjected to an electric field within a pH gradient, which causes them to migrate to the point in the gradient where their net charge is zero (their PI). Different proteins have different PIs and will therefore migrate to distinct positions in the gel. Isoelectric focusing provides high resolution separation and is useful for research applications such as taxonomy, cytology and immunology.
fluroscence spectroscopy
Fluorescence spectroscopy involves using ultraviolet light to excite electrons in molecules, causing them to emit visible light. The emitted light has a longer wavelength than the absorbed light. Fluorimeters are used to measure fluorescence, exciting samples at an absorption wavelength and measuring emission at a longer fluorescence wavelength. Fluorescence spectroscopy is useful for applications like determining fluorescent drugs in formulations, carrying out limit tests for fluorescent impurities, and studying drug-protein binding in bioanalysis.
Chromatography
Chromatography is a technique used to separate and identify the components of a mixture. It works by allowing the molecules present in the mixture to distribute themselves between a stationary and a mobile medium. Molecules that spend most of their time in the mobile phase are carried along faster. There are different types of chromatography classified according to the mobile phase used and the packing of the stationary phase. Chromatography techniques include thin layer chromatography, paper chromatography, column chromatography, gas chromatography and liquid chromatography. These techniques find application in various fields to analyze sample mixtures.
Thin layer Chromatography (TLC)
Thin layer chromatography is a technique used to separate mixtures into their components. It involves a stationary phase, such as silica gel, and a mobile phase, such as a solvent. A sample is applied to the plate and the mobile phase moves up the plate, separating the components by their interaction with the stationary phase. The separated components are then detected and quantified. TLC is useful for analyzing mixtures and is simpler and less expensive than other chromatography methods.
Classification of Chromatography
This document provides information about chromatography. It defines chromatography as a method of separation where components are distributed between a stationary and mobile phase. The stationary phase can be solid or liquid, and the mobile phase can be liquid, gas, or supercritical fluid. Various types of chromatography are described based on the interaction between components and phases, including thin layer chromatography, column chromatography, gas chromatography, and liquid chromatography. Key applications and principles of different chromatographic techniques are also summarized.
chromatographic techniques
Chromatography is a technique used to separate and identify the components of a mixture. It works by allowing molecules to distribute themselves between a stationary and mobile phase, so that molecules that interact more with the mobile phase move faster. Chromatographic techniques can be classified based on the interaction with the stationary phase or physical state of the mobile phase. Key techniques include adsorption, partition, ion exchange, exclusion, gas, liquid, and thin layer chromatography. Proper sample preparation and development conditions are important for achieving optimal separation and resolution of components in the mixture.
Classification of chromatography
Chromatography is a method of separating mixtures into individual components using a stationary and mobile phase. There are several types depending on the physical state of the phases and interaction between the phases and components. Liquid chromatography uses a liquid mobile phase passing through a solid or liquid stationary phase to separate components. Gas chromatography uses a gas mobile phase to separate volatile components. Size exclusion and ion exchange chromatography separate based on molecular size or charge.
chromatography
Chromatography is a technique used to separate mixtures by distributing components between two phases - a stationary phase and a mobile phase. Mikhail Tswett discovered chromatography in 1906 when separating plant pigments. There are various types of chromatography classified by mobile phase (liquid, gas), stationary phase material (thin layer, paper, column), or separation mechanism (adsorption, partition, ion exchange, size exclusion, affinity). Chromatography has many applications in science and industry, including purification of antibiotics, vaccines, enzymes, and other biomolecules.
Paper Chromatography
Chromatography is a technique used to separate mixtures into individual components. It works by using the differential affinity and solubility of components for both a mobile phase that carries the mixture and a stationary phase that the mixture passes through. The separation of components depends on how strongly they interact with each phase. Common types of chromatography include liquid chromatography, gas chromatography, paper chromatography, and thin-layer chromatography. Paper chromatography can be used to separate the dyes in markers by running samples along a strip with an increasing solvent mixture as the mobile phase.
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
This document provides an introduction to high performance liquid chromatography (HPLC). It discusses the basic components and principles of HPLC, including the stationary and mobile phases, pumps to move the mobile phase through the column at high pressure, and various detectors used to analyze the separated components as they elute from the column. It also describes the different types of HPLC based on the mode of separation, elution technique, scale of operation, and type of analysis performed.
Size exclusion chromatograpy
Size exclusion chromatography, also known as gel filtration chromatography, separates molecules based on their size and molecular weight. Larger molecules pass through the porous beads of the stationary phase more quickly than smaller molecules that can enter the pores. This document discusses the basic principles, history, applications, and factors affecting size exclusion chromatography such as column length and packing. It is commonly used to purify proteins and other biomolecules.
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2. The key components of an HPLC system are the solvent reservoirs, pump, injector, column, and detector. HPLC can be used for both analytical and preparative purposes to separate, purify, identify, and quantify compounds.
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HPLC is a separation technique that uses high pressure to force a liquid mobile phase through a column packed with solid particles. The components to be separated are distributed between the mobile and stationary phases based on properties like polarity. Key components of an HPLC system include an injector, pump, column, and detector. HPLC has many applications such as clinical testing by analyzing compounds in blood/urine, environmental analysis of pollutants, forensic drug quantification, and food quality testing.
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HPLC is a type of liquid chromatography that can separate mixtures of chemicals. It works by pumping a pressurized liquid solvent (mobile phase) through a column containing a solid material (stationary phase). Samples are injected and the different compounds interact differently with the phases, causing them to elute from the column at different retention times, allowing separation. HPLC has advantages over other methods like higher separation efficiency, reproducibility, and ability to analyze a wide range of compounds dissolved in liquid. It is used in various fields like medicine, food, environment, and industry.
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HPLC is a type of column chromatography that uses high pressure to pass a sample mixture in a mobile liquid phase through a column containing a stationary solid phase, allowing the components to separate. It provides high resolution and sensitivity for analyzing chemicals and biological molecules. Key components include a solvent reservoir, pump, injector, column, detector, and recorder. Separation occurs as each component interacts differently with the stationary phase based on properties like polarity and solubility. The detector measures the components as they elute from the column, generating a chromatogram to identify the components. HPLC has many applications in fields like pharmaceuticals, chemicals, foods, biosciences, and more.
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This document provides an overview of fundamentals of high performance liquid chromatography (HPLC). It explains that HPLC involves injecting a liquid sample into a column packed with tiny particles and using a liquid mobile phase to separate the sample components. The major components of an HPLC system are identified as the pump, injector, column, detector and computer. Common applications of HPLC include qualitative analysis to identify compounds, quantitative analysis to measure amounts, and trace analysis to detect very low concentration compounds.
HPLC
HPLC is a liquid chromatography technique used to separate mixtures of compounds. It involves injecting a sample into a column packed with porous particles, through which a liquid mobile phase is pumped under high pressure. The separated components interact differently with the stationary phase and elute from the column at different retention times, producing a chromatogram. HPLC is used for qualitative and quantitative analysis of compounds, identification of unknown mixtures, and preparation of pure samples. It is well-suited for separation of non-volatile and thermally-fragile compounds across various application areas.
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HPLC is a form of liquid chromatography that uses high pressure to generate flow through a column packed with small particles. It allows for efficient separation of compounds based on differences in how they interact with the stationary and mobile phases. Key aspects of HPLC include pumps to deliver mobile phases at high pressure, injectors for sample introduction, columns packed with particles or beads, detectors to identify eluting compounds, and data systems to analyze results. Common modes are reverse phase, normal phase, size exclusion, and ion exchange chromatography. HPLC finds wide application in fields like pharmaceuticals, biochemistry, and environmental analysis.
HPLC
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This document provides an overview of high performance liquid chromatography (HPLC). It describes the key components of an HPLC system including the stationary phase, mobile phase, injector, chromatographic column, pumping system, and detectors. It explains the separation process, noting that differences in how compounds partition between the mobile and stationary phases allows for separation. It also discusses normal phase and reverse phase chromatography, and provides examples of applications such as pharmaceutical analysis, food and flavor testing, and environmental and clinical analysis.
High-Performance Liquid Chromatography (HPLC) ppt.pptx
HPLC is a popular and versatile technique for separating, identifying, and quantifying constituents of complex organic samples. It works by forcing a pressurized mobile phase through a column containing a stationary phase, which interacts differently with different compounds and allows them to be separated. Key components of an HPLC system include the pump, injector, column, detector, and data analysis software. HPLC can separate compounds based on differences in how strongly they interact with the mobile and stationary phases.
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This document discusses High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC).
HPLC uses high pressure to push a mobile phase through a column packed with small particles to separate compounds dissolved in solution based on their affinity to the stationary phase. It has components like pumps, injectors, columns, and detectors. GC vaporizes samples and uses an inert gas as the mobile phase to separate compounds in the gas phase based on their boiling points as they elute from the column. Both techniques are used to qualitatively analyze mixtures by detecting separated components.
Hplc high performance liquid chromatography
HPLC is a chromatographic technique used to separate components in a mixture. It works by pumping a pressurized mobile liquid phase through a column containing a stationary phase, which causes the components in a sample to separate as they are transported through the column at different rates. Key components of an HPLC system include solvent reservoirs, pumps to precisely deliver the mobile phase, an injector to introduce samples, columns for separation, detectors, and a data system to analyze results. HPLC offers advantages like high separation capacity, reproducibility, and ability to analyze a wide range of biological, medical, food, environmental, and industrial samples.
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY/SAGAR/SHARMA/DEPARTMENT OF PHARMACEUTI...
High performance liquid chromatography is a technique to get analytical information from a small amount of sample.
HPLC
HPLC
Chromatography
Mobile Phase & Stationary Phase
CLASSIFICATION OF CHROMATOGRAPHY
Characteristics of HPLC
Purpose
Superiority of HPLC
TYPES OF HPLC TECHNIQYES
Principle
PHASING SYSTEM & (normal vs reversed phase)
INSTRUMENTATION
Flow diagram of HPLC instrument
Advantages of HPLC
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High performance liquid chromatography
- 1. High Performance Liquid Chromatography GIVEN BY : SYED MURTAZA SHAH ROLL NO : 16CH48
- 2. SUBTOPICS : • Chromatography Definition • History of Chromatography • Working Principle of Chromatography • Types of Chromatography • High Performance Liquid Chromatography(HPLC) • Working, Parts and Applications of HPLC SYSTEM
- 4. How does it Works??
- 5. Simple Experiment to understand Chromatography
- 7. Experiment of M.S Tswett
- 8. Basic Termologies used in Chromatography Term Definition Mobile phase or carrier solvent moving through the column Stationary phase or adsorbent substance that stays fixed inside the column Eluent fluid entering the column Eluate fluid exiting the column (that is collected in flasks) Elution the process of washing out a compound through a column using a suitable solvent Analyte mixture whose individual components have to be separated and analyzed
- 10. Working Principle of Chromatography
- 11. Chromatography works on Adsorption Principle
- 17. Parts of Hplc System
- 18. Working of HPLC system
- 19. How does the User knows what and how many types of Elements collected form sample
- 20. Scales of HPLC
- 22. THANK YOU