Drosophila melanogaster, commonly known as the fruit fly, is a widely used model organism in biological research. It has four pairs of chromosomes and a fully sequenced genome of around 139.5 million base pairs containing over 15,000 genes. Many human disease genes have homologs in fruit flies, making Drosophila useful for studying mechanisms underlying neurodegenerative diseases, aging, immunity, and cancer. Hox genes are important transcription factors that determine body plan along the head-to-tail axis. Drosophila has two Hox gene complexes containing eight Hox genes that control segment identity and structure formation. Loss or misexpression of Hox genes can lead to homeotic transformations where one body part develops in the place of another
Transfection methods (DNA to host cell) Erin Davis
Transfection of DNA to host cell can be done by various methods in lab scale.Gene gun,electroporation,lipofection .These methods are used to transfer DNA to the host cell.
this section helps students how to quanify the isolated DNA by spectrophotometer. specially life life science fields such as biotechnology, biology, and medical laboratory
Transfection methods (DNA to host cell) Erin Davis
Transfection of DNA to host cell can be done by various methods in lab scale.Gene gun,electroporation,lipofection .These methods are used to transfer DNA to the host cell.
this section helps students how to quanify the isolated DNA by spectrophotometer. specially life life science fields such as biotechnology, biology, and medical laboratory
Gene regulation in eukaryotes in a nutshell covering all the important stages of gene regulation in eukaryotes at transcriptional level, translation level and post-translational level.
The technique of molecular biology like DNA isolation, RNA isolation, PCR, Western blot, RFLP, etc was developed with development in science. This presentation includes the method of DNA and RNA isolation and their Quantification techniques.
Segmentation in Drosophila melanogaster Shreya Ahuja
All human beings, no matter how different we look, have a certain basic body plan established in us (for instance, all of us have our heads are placed right above our shoulders with arms stretching out from either side). Drosophila is no exception. This presentation talks about establishment of the body plan in Drosophila, how and when the different Segmentation Genes are expressed in Drosophila to give rise to its segmented body pattern.
Effective disruption of the biological matrix (cell, tissue, environmental or biological sample) to release the nucleic acids. Denaturation of structural proteins associated with the nucleic acids (nucleoproteins) Inactivation of nucleases that will degrade the isolated product (RNase and/or DNase).
Once the genomic DNA is bound to the silica membrane, the nucleic acid is washed with a salt/ethanol solution. These washes remove contaminating proteins, lipopolysaccharides and small RNAs to increase purity while keeping the DNA bound to the silica membrane column.
There are five basic steps of DNA extraction that are consistent across all the possible DNA purification chemistries:
disruption of the cellular structure to create a lysate,
separation of the soluble DNA from cell debris and other insoluble material,
binding the DNA of interest to a purification matrix,
washing proteins and other contaminants away from the matrix and
elution of the DNA.
SDS-PAGE is a technique used to separate proteins .
The concept of stacking and resolving gel in this SDS-PAGE are briefly describe in very interesting and easy way to understand the role of stacking and resolving gel.
Gene regulation in eukaryotes in a nutshell covering all the important stages of gene regulation in eukaryotes at transcriptional level, translation level and post-translational level.
The technique of molecular biology like DNA isolation, RNA isolation, PCR, Western blot, RFLP, etc was developed with development in science. This presentation includes the method of DNA and RNA isolation and their Quantification techniques.
Segmentation in Drosophila melanogaster Shreya Ahuja
All human beings, no matter how different we look, have a certain basic body plan established in us (for instance, all of us have our heads are placed right above our shoulders with arms stretching out from either side). Drosophila is no exception. This presentation talks about establishment of the body plan in Drosophila, how and when the different Segmentation Genes are expressed in Drosophila to give rise to its segmented body pattern.
Effective disruption of the biological matrix (cell, tissue, environmental or biological sample) to release the nucleic acids. Denaturation of structural proteins associated with the nucleic acids (nucleoproteins) Inactivation of nucleases that will degrade the isolated product (RNase and/or DNase).
Once the genomic DNA is bound to the silica membrane, the nucleic acid is washed with a salt/ethanol solution. These washes remove contaminating proteins, lipopolysaccharides and small RNAs to increase purity while keeping the DNA bound to the silica membrane column.
There are five basic steps of DNA extraction that are consistent across all the possible DNA purification chemistries:
disruption of the cellular structure to create a lysate,
separation of the soluble DNA from cell debris and other insoluble material,
binding the DNA of interest to a purification matrix,
washing proteins and other contaminants away from the matrix and
elution of the DNA.
SDS-PAGE is a technique used to separate proteins .
The concept of stacking and resolving gel in this SDS-PAGE are briefly describe in very interesting and easy way to understand the role of stacking and resolving gel.
hox genes and its role in development both in human and drosophila . homeotic genes. homeobox genes. developmental biology. different types of homeotic genes in drosophila and human. deficiencydiseases due to lack of hox genes in human
Weinstock et al. (81 authors), Gillespie J.J., Cannone J.J., Gutell R.R., et al. (100 authors) (2006).
Insights into social insects from the genome of the honeybee Apis mellifera.
Nature, 443(7114):931-949.
Toxic effects of heavy metals : Lead and Arsenicsanjana502982
Heavy metals are naturally occuring metallic chemical elements that have relatively high density, and are toxic at even low concentrations. All toxic metals are termed as heavy metals irrespective of their atomic mass and density, eg. arsenic, lead, mercury, cadmium, thallium, chromium, etc.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
3. DROSOPHILA MELANOGASTER:-
Drosophila melanogaster is a species of Fly (the taxonomic order Diptera) in the family
Drosophilidae. The species is known generally as the common fruit fly or vinegar fly. Starting
with Charles W. Woodworth's proposal of the use of this species as a model organism, D.
melanogaster continues to be widely used for biological research in studies of genetics,
physiology, microbial pathogenesis and life history evolution. It is typically used because it is
an animal species that is easy to care for, has four pairs of chromosomes, breeds quickly, and
lays many Drosophila melanogaster eggs. D.melanogaster is a common pest in homes,
restaurants, and other occupied places where food is served.
Flies belonging to the family Tephritidae are also called fruit flies, which can lead to confusion,
especially in Australia and South Africa, where the term fruit fly refers to members of the
Tephritidae that are economic pests in fruit production, such as Ceratitis capitata, the
Mediterranean fruit fly or "Medfly".
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9. Genome of drosophila:-
The genome of D. melanogaster (sequenced in 2000, and curated at the Fly Base
database contains four pairs of chromosomes: an X/Y pair, and three autosomes
labeled 2, 3, and 4. The fourth chromosome is so tiny that it is often ignored,
aside from its important eyeless gene. The D. melanogaster sequenced genome of
139.5 million base pairs has been annotated and contains approximately 15,682
genes according to Ensemble release 73. More than 60% of the genome appears to
be functional non-protein- coding DNA involved in gene expression control.
Determination of sex in Drosophila occurs by the X:A ratio of X chromosomes to
autosomes, not because of the presence of a Y chromosome as in human sex
determination. Although the Y chromosome is entirely heterochromatic, it contains
at least 16 genes, many of which are thought to have male-related functions.
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11. Similarity to humans:-
About 75% of known human disease genes have a recognizable
match in the genome of fruit flies, and 50% of fly protein
sequences have mammalian homologs. An online database
called Homophila is available to search for human disease gene
homologues in flies and vice versa. Drosophila is being used as
a genetic model for several human diseases including the
neurodegenerative disorders Parkinson's, Huntington's,
spinocerebellar ataxia and Alzheimer's disease. The fly is also
being used to study mechanisms underlying aging and oxidative
stress, immunity, diabetes, and cancer, as well as drug abuse.
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21. HOX GENE:-
Hox genes (also known as homeotic genes) are a group of related genes that
control the body plan of an embryo along the anterior-posterior (head-tail) axis.
After the embryonic segments have formed, the Hox proteins determine the type of
segment structures(e.g. leg, antennae, and wings in fruit flies or the different types
of vertebrae in humans) that will form on a given segment. Hox proteins thus
confer segmental identity, but do not form the actual segments themselves.
Hox genes are defined as having the following properties:
•their protein product is a transcription factor
•they contain a DNA sequence known as the homeohox
•in many animals, the organization of the Hox genes on the chromosome is the
same as the order of their expression along the anterior-posterior axis of the
developing animal, and are thus said to display colinearity
22. Hox gene function in Drosophila
Drosophila melanogaster is an important model for understanding body
plan generation and evolution.The general principles of Hox gene
function and logic elucidated in flies will apply to all Bilaterian
organisms, including humans.Drosophila, like all insects,has 8 Hox
genes.These are clustered into two complexes, both of which are
located on chromosome 3.The Antennapedia complex (not to be
confused with the Antp gene) consists of 5 genes: labial (lab),
proboscipedia (pb), Deformed (Dfd) Sex combs reduced (Ser) and
Antennapedia (Antp). The Bithorax Homeohox gene expression
inDrosophi la melanogaster complex, named after the Ultrabithorax
gene, consists of the remaining 3 genes:Ultrabithorax (Ubx),abdominal-
A (abd-A) and Abdominal-B (abd-B).
23. lab (labial)
The lab gene is the most anteriorly expressed gene. It is expressed in the head, primarily in the
intercalary segment (an appendage-less segment between the antenna and mandible), and also in
the midgut. Loss of function of lab results in the failure of the Drosophila embryo to internalize
the mouth and head structures that initially develop on the outside of its body (a process called
head involution). Failure of head involution disrupts or deletes the salivary glands, pharynx. The
lab gene was initially so named because it disrupted the labial appendage; however, the lab gene is
not expressed in the labial segment, and the labial appendage phenotype is likely a result of the
broad disorganization resulting from the failure of head involution.
pb (proboscipedia)
The pb gene is responsible for the formation of the labial and maxillary palps. There is evidence
that pb interacts with Scr.
24. Dfd (Deformed)
The Dfd gene is responsible for the formation of the maxillary and mandibular segments in the
larval head. The mutant phenotypes of Deformed are similar to those of labial. Loss of function
of Deformed in the embryo results in a failure of head involution (see labial gene), with a loss of
larval head structures. Mutations in the adult have either deletions of parts of the head or
transformations of head to thoracic identity.
Ser (Sex combs reduced)
The Ser gene is responsible for cephalic and thoracic development in Drosophila embryo and
adult.
Antp (Antennapedia)
The 2nd thoracic segment, or T2, develops a pair of legs and a pair of wings. The Antp gene
specifies this identity by promoting leg formation and allowing (but not directly activating) wing
formation. A dominant Antp mutation, caused by a chromosomal inversion, causes Antp to be
expressed in the antenna! imaginal disc, so that, instead of forming an antenna, the disc makes a
leg, resulting in a leg coming out of the fly's head
25. Ubx (Ultrabithorax)
The third thoracic segment, or T3, bears a pair of legs and a pair of halteres (highly reduced
wings that function in balancing during flight). Ubx patterns T3 largely by repressing genes
involved in wing formation. The wing blade is composed of two layers of cells that adhere
tightly to one another, and are supplied with nutrient by several wing veins. One of the many
genes that Ubx represses is blistered, which activates proteins involved in cell-cell adhesion, and
spalt, which patterns the placement of wing veins. In Ubx loss-of-function mutants, Ubx no
longer represses wing genes, and the halteres develop as a second pair of wings, resulting in the
famous four-winged flies. When Ubx is misexpressed in the 2nd thoracic segment, such as
occurs in flies with the "Cbx" enhancer mutation, it represses wing genes, and the wings develop
as halteres, resulting in a four-haltered fly.
26. abd-A (abdominal-A)
In Drosophila, abdominal-A (abd-A) is expressed along most of the abdomen, from abdominal
segment 1 (Al) to A8. Expression of abdominal-A is necessary to specify the identity of most of
the abdominal segments. A major function of abd-A in insects is to repress limb formation. In
abd-A loss-of-function mutants, abdominal segments A2 through A8 are transformed into an
identity more like Al. When abd A is ectopically expressed throughout the embryo, all segments
anterior of A4 are transformed to an A4- like abdominal identity The abd-A gene also affects the
pattern of cuticle generation in the ectoderm, and pattern of muscle generation in the mesoderm.
Abd-B (Abdominal-B)
Abd-B is transcribed in two different forms, a regulatory protein, and a morphogenic protein.
Regulatory Abd-B suppress embryonic ventral epidermal structures in the 8th and 9th segment
of the Drosophila abdomen. Both the regulatory protein, and the morphogenic protein are
involved in the development of the tail segment.