Gas chromatography is a technique used to separate components in a mixture using an inert gas as the mobile phase and a stationary phase in the column. Key aspects of gas chromatography include the carrier gas, sample injection, columns with solid or liquid stationary phases, temperature programming, and detectors like FID, TCD, ECD that measure separated components. Gas chromatography provides sensitive, precise, and accurate analysis of mixtures like drugs, foods, pollutants, and more within a short time.
Introduction to gas Chromatography
,Principle of gas chromatography
Instrumentation of gas Chromatography
Type of detectors of gas chromatography
Advantages of gas chromatography
Disadvantages of gas chromatography
Applications of gas chromatography
Hii..
in which slide we are involving what is Gas chromatography there History, Theory & principle, Introduction, Phases, Types, Instrumentation, Application etc.
Gas chromatography and its instrumentationArgha Sen
Gas chromatography is an unique technology which helps us in separating volatile analytes. Its is an easy and reproduciple method for detecting residual solvents found in APIs.
in this slides contains principle and types of detectors used in Gas Chromatography.
Presented by: J.Vinay Krishna. (Department of industrial pharmacy),
RIPER, anantapur.
In this slide contains principle of IR spectroscopy and sampling techniques.
Presented by: R.Banuteja (Department of pharmaceutical analysis).
RIPER, anantpur.
Gas chromatography head points:
Invention of Chromatography
original chromatography Experiment
Common types of chromatography
Paper and Thin layer chromatography
How does chromatography work?
Theoretical Plate
gas chromatography
schematic of GC
carrier gas-supply
Injection port
sample Injection system
split/spitless Injection
sample valves
GC columns
open tubular columns
Temperature Control
Solid Support Materials
Particle size of Supports
The stationary Phase
Detection systems
Characteristics of the Ideal Detector
Flame Ionization Detectors
Thermal Conductivity Detector
Electron-capture Detectors
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fluid chromatography (SFC) can be used on an analytical
scale.
It is a combination of High performance liquid chromatography (HPLC)
and Gas chromatography (GC).
It can be used with non-volatile and thermally labile analytes.
It can be used with the universal flame ionization detector.
It is important to producing narrower peaks due to rapid diffusion.
It is important for the chiral separations and analysis of high-molecularweight
hydrocarbons.
Supercritical fluids are suitable as a substitute for organic solvents in a
range of industrial and laboratory processes.
Low amount of sample
Complex mixture.
Gas chromatography is a process of separating component(s) from the given crude drug or mixture by using stationary phase (solid or liquid) and gaseous mobile phase. It involves a sample being vaporized and injected onto the head of the chromatographic
column. The sample is transported through the column by the flow of inert, gaseous
mobile phase. The column itself contains a solid or liquid stationary phase which is adsorbed onto the
surface of an inert solid.
Gas Chromatography in Analytical Analysis.pptxRAHUL PAL
Gas chromatography is a common type of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition. Typical uses of GC include testing the purity of a particular substance, or separating the different components of a mixture.
Introduction to gas Chromatography
,Principle of gas chromatography
Instrumentation of gas Chromatography
Type of detectors of gas chromatography
Advantages of gas chromatography
Disadvantages of gas chromatography
Applications of gas chromatography
Hii..
in which slide we are involving what is Gas chromatography there History, Theory & principle, Introduction, Phases, Types, Instrumentation, Application etc.
Gas chromatography and its instrumentationArgha Sen
Gas chromatography is an unique technology which helps us in separating volatile analytes. Its is an easy and reproduciple method for detecting residual solvents found in APIs.
in this slides contains principle and types of detectors used in Gas Chromatography.
Presented by: J.Vinay Krishna. (Department of industrial pharmacy),
RIPER, anantapur.
In this slide contains principle of IR spectroscopy and sampling techniques.
Presented by: R.Banuteja (Department of pharmaceutical analysis).
RIPER, anantpur.
Gas chromatography head points:
Invention of Chromatography
original chromatography Experiment
Common types of chromatography
Paper and Thin layer chromatography
How does chromatography work?
Theoretical Plate
gas chromatography
schematic of GC
carrier gas-supply
Injection port
sample Injection system
split/spitless Injection
sample valves
GC columns
open tubular columns
Temperature Control
Solid Support Materials
Particle size of Supports
The stationary Phase
Detection systems
Characteristics of the Ideal Detector
Flame Ionization Detectors
Thermal Conductivity Detector
Electron-capture Detectors
https://www.linkedin.com/in/preeti-choudhary-266414182/
https://www.instagram.com/chaudharypreeti1997/
https://www.facebook.com/profile.php?id=100013419194533
https://twitter.com/preetic27018281
Please like, share, comment and follow.
stay connected
If any query then contact:
chaudharypreeti1997@gmail.com
Thanking-You
Preeti Choudhary
fluid chromatography (SFC) can be used on an analytical
scale.
It is a combination of High performance liquid chromatography (HPLC)
and Gas chromatography (GC).
It can be used with non-volatile and thermally labile analytes.
It can be used with the universal flame ionization detector.
It is important to producing narrower peaks due to rapid diffusion.
It is important for the chiral separations and analysis of high-molecularweight
hydrocarbons.
Supercritical fluids are suitable as a substitute for organic solvents in a
range of industrial and laboratory processes.
Low amount of sample
Complex mixture.
Gas chromatography is a process of separating component(s) from the given crude drug or mixture by using stationary phase (solid or liquid) and gaseous mobile phase. It involves a sample being vaporized and injected onto the head of the chromatographic
column. The sample is transported through the column by the flow of inert, gaseous
mobile phase. The column itself contains a solid or liquid stationary phase which is adsorbed onto the
surface of an inert solid.
Gas Chromatography in Analytical Analysis.pptxRAHUL PAL
Gas chromatography is a common type of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition. Typical uses of GC include testing the purity of a particular substance, or separating the different components of a mixture.
Gas Chromatography is an analytical techniques, used for the separation of volatile substances on the basis of their partition coefficient . In this slide you will find out different instrumentation of gas chromatography, its advantages, disadvantages and moreover its applications.
Gas chromatography- “It is a process of separating component(s) from the given crude drug by using a gaseous mobile phase.”
Principle- The principle of separation in GC is “partition.”
The mixture of components to be separated is converted to vapor and mixed with the gaseous mobile phase.
The component which is more soluble in the stationary phase travels slower and eluted later.
The component which is less soluble in the stationary phase travels faster and eluted out first.
No two components have the same partition coefficient conditions.
So the components are separated according to their partition coefficient.
The partition coefficient is “the ratio of solubility of a substance distributed between two immiscible liquids at a constant temperature.’
It involves a sample being vaporized and injected onto the head of the chromatographic column.
The sample is transported through the column by the flow of inert, gaseous mobile phase.
The column itself contains a liquid stationary phase which is adsorbed onto the surface of an inert solid.
Two major types:
1. gas-solid chromatography: Here, the mobile phase is a gas while the stationary phase is a solid.
Used for separation of low molecular gases,
e.g., air components, H2S, CS2, CO2, rare gases, CO, and oxides of nitrogen.
2.Gas-liquid chromatography: The mobile phase is a gas while the stationary phase is a liquid retained on the surface as an inert solid by adsorption or chemical bonding.
Advantages-
Both qualitative and quantitative analyses are possible.
The instrument is simple, time of analysis is short.
High sensitivity.
The method is applicable to about 60% of organic compounds.
Very small sample sizes can be used.
Analysis can be highly accurate and precise.
Applications-
Quality control and analysis of drug products like antibiotics (penicillin), antivirals (amantadine), general anesthetics (chloroform, ether), sedatives/hypnotics (barbiturates), etc.
Assay of drugs – purity of a compound can be determined for drugs like :
Atropine sulfate
Clove oil
Stearic acid
In determining the levels of metabolites in body fluids like plasma, serum, urine, etc
Estimation of spoilage components, such as histamine and carbonyls, that cause rancidity.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
How to Create Map Views in the Odoo 17 ERPCeline George
The map views are useful for providing a geographical representation of data. They allow users to visualize and analyze the data in a more intuitive manner.
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
1. GAS CHROMATOGRAPHY
Prepared By:-
Mr. Lokesh Thote
Asstt. Professor
Department of Pharmaceutical
Chemistry
Kamla Nehru
College of Pharmacy,
Butibori, Nagpur (M.S.)
2. Introduction
* Gas chromatography – It is a process of separating
component(s) from the given crude drug or mixture by using
stationary phase (solid or liquid) and gaseous mobile phase.
* It involves a sample being vaporized and injected onto the head of
the chromatographic column. The sample is transported through the
column by the flow of inert, gaseous mobile phase.
* The column itself contains a solid or liquid stationary phase which
is adsorbed onto the surface of an inert solid.
* Two major types
• Gas-solid chromatography (GSC)
(Stationary phase: solid)
• Gas-liquid chromatography (GLC)
(Stationary phase: immobilized liquid)
3. Advantages of Gas Chromatography
* The technique has strong separation power and even complex
mixture can be resolved into constituents
* The sensitivity of the method is quite high. it is micro method only
few mg of sample is sufficient for analysis.
* It gives good precision and accuracy
* The analysis is completed in a short time
* The cost of instrument is relatively low and its life is generally long
* The technique is relatively suitable for routine analysis (do not
require highly skilled person for operation)
4. Components of Gas chromatograph.
1. Carrier gas:- He (common), N2, H2, Argon.
2. Flow regulators & Flow meters
Sample injection port:-
Columns:- 2-50 m coiled stainless steel/glass/Teflon.
3. Detectors:- Flame ionization (FID), Thermal conductivity
(TCD), Electron capture (ECD), Nitrogen-phosphorus, Flame
photometric (FPD), Photo-ionization (PID).
4. Thermostat chamber:- for temperature regulation of the
column and detectors.
5. An amplifier and recording system
7. 1. Carrier gas
The carrier gas must be chemically inert.
Commonly used gases include hydrogen, nitrogen, helium, argon, and
carbon dioxide.
Hydrogen has more advantages as compared to others but it highly
flammable.
Helium is generally used because of its excellent thermal
conductivity, inertness, low density and allow high flow rate.
The choice of carrier gas is often dependant upon the type of detector
which is used.
For selecting carrier following thing should be consider
o It should be inert
o Should be suitable for detectors. No fire or explosion hazards
o Readily available in high purity. Should be cheap.
8. 2. Flow regulators & Flow meters
Deliver the gas with uniform pressure/flow rate
flow meters:- Rota meter & Soap bubble flow meter
ROTA METER
Placed before column inlet
It has a glass tube with a float
held on to a spring.
The level of the float is
determined by the flow rate
of carrier gas
9. SOAP BUBBLE METER
Similar to Rota meter but instead of a float, soap bubble formed
indicates the flow rate
10. 3. Sample injection port:
oThe most common method of sample injection involves the use of
micro syringe to inject a liquid or gaseous sample through a self
sealing silicon rubber diaphragm or septum into a flash vaporizer
port located at the head of column.
oThe sample port is generally
located abut the 500 c above the
boiling point of least volatile
component.
oTo increase the column efficiency
suitable size of sample should be
selected and introduced as a plug of
vapor. The slow injection of over
size sample causes band broadening
and poor resolution of a mixture
11. 4. Gas Chromatography – Columns
The column is heart in chromatography. In the column the
different solute in the vaporized sample separated from each other
by virtue of their different interaction with the column packing .
The two types of column are commonly employed in gas
chromatography.
1. Packed column
2. Capillary column
1. Packed columns
are prepared by packing metal are glass tubing with
finely divided, inert, solid support material (diatomaceous
earth) for GSC.
Solid inert coated with liquid stationary phase for GLC.
Most packed columns are 1.5 – 10 m in length and have an
internal diameter of 2 - 4mm.
12. 2. Capillary columns
This column also referred as tubular column
Made up of long capillary tubing (30 to 90 meter) in length
having uniform and narrow internal diameter (0.025 – 0.075 cm).
Made up of stainless steel, copper, nylon or glass etc.
Inside the wall of capillary tubing is coated with the liquid
phase in the form of thin (0.5 – 1 micron) uniform film on inert
solid support or coated with inert solid material.
There are two type of capillary column
Open tubular column
Support coated open tubular column
15. 5. G C - DETECTORS
There are many detectors which can be used in gas chromatography
Different detectors will give different types of selectivity.
* Detectors can be grouped into concentration dependant
detectors and mass flow dependant detectors.
* The signal from a concentration dependant detector is related to the
concentration of solute in the detector, and does not usually destroy
the sample Dilution of with make-up gas will lower the detectors
response.
* Mass flow dependant detectors usually destroy the sample, and the
signal is related to the rate at which solute molecules enter the
detector. The response of a mass flow dependant detector is
unaffected by make-up gas
16. G C – IDEAL DETECTORS
Sensitive (10-8-10-15 g solute/s)
Operate at high T (0-400 °C) S
table and reproducible Linear response
Wide dynamic range
Fast response Simple (reliable)
Nondestructive
Uniform response to all analytes
17. 1. Thermal Conductivity Detector (TCD)
The principle of the detector is that the temperature and thus resistance
of the wire through which current is flowing is depend upon thermal
conductivity of gas in which it is immersed.
Thermal conductivity of gas is a function of its composition.
Construction & Working
It consist of two chamber of small volumes, made within a metal block:
each containing a resistance wire with high temperature coefficient of
resistance (resistance varies greatly with temperature). These resistance
constitute Reference (R) and sensing (S).
The carrier gas is passes in both the cell and arrangement is such that the
column effluent are moved into the sensing side only.
When a sample component enters into sensing cell, the temperature of the
sensing filament S changes due to widely different thermal conductivity of
sample component than the carrier gas, as a result resistance of S also varies
and bridge become unbalanced. This off-balance current is signalled to
recorder which draws the elution curve for chromatographic separation.
19. 2. Flame Ionization Detector (FID)
Principle: The ionization detector are based on the electrical conductivity
of gases.
At normal temperature and pressure gases act as insulator but become
conductive of ions if electrons are present. If this condition are such that
the gas molecule themselves do not ionise, but change in conductivity due
to presence of a very small number of ions can be detected.
Construction and Working: Hydrogen gas is added with a capillary jet if
it was not used as a carrier gas, the sample components from effluent
are burn in air (or oxygen, hydrogen) and ionized.
These ions raise upwards and are attracted towards anode or cathode
based on charge on them.
When they impinge on the electrodes, current is passed which is recorded.
The strength and intensity of current depends on the sample its
concentration
21. 3. Electron Capture Detector (ECD)
Principle
The electron affinity of different substances can be used as basis
for ionization detector called as Electron capture detector (ECD)
This detector depend to only those compounds whose molecule
have an affinity for electrons e. g. chlorinated compounds, alkyl,
lead etc.
Construction and working
ECD consist of chamber in which metal foil coated with a tritium
(radioactive β emitter) is used as steady source of slow electrons.
The ECD is most frequently used for detection and measurement
of trace environmental pollutants.
It has high sensitivity for halogenated compounds therefore used
for detection of herbicides pesticides, traces in fuel gases,
organometallic compounds etc.
23. 4. Argon ionization detector;
These detectors are similar to flame ionization detectors
with only difference that argon ion gas is used to ionize the
sample molecules.
The argon ion are obtained by reacting argon gas with
radio active elements. Once argon ionizes they try to get
back to stable state by either taking or giving electron from
the sample components thus making sample molecules to
ions for detection.
24. Summary of common GC detectors
Detector Type
Flame ionization (FID) Mass flow
Support gases Selectivity Detectability Dynamic range
Hydrogen and air Most organic cpds. 100 pg 107
Thermal conductivity
(TCD)
Concentration Reference Universal 1 ng 107
Electron capture (ECD) Concentration Make-up
Halides, nitrates,
nitriles, peroxides,
anhydrides,
organometallics
50 fg 105
Nitrogen-phosphorus Mass flow Hydrogen and air Nitrogen, phosphorus 10 pg 106
Flame photometric
(FPD)
Mass flow
Hydrogen and air
possibly oxygen
Sulphur, phosphorus,
tin, boron, arsenic,
germanium, selenium,
chromium
100 pg 103
Photo-ionization (PID) Concentration Make-up
Aliphatics, aromatics,
ketones, esters,
aldehydes, amines,
heterocyclics,
organosulphurs, some
organometallics
2 pg 107
25. 6. Temperature Programming System
As temperature programming facilitates controlled increase of
even temperature during analysis. So that peaks of analytes
should be sharp and emerge quickly.
Because of temperature programming facility the components
of wide boiling point range mixture may be resolved efficiently.
Temperature programming may be carried out by three different
modes
Natural or ballastic
Linear
Matrix or multilinear
26. * Application of Gas Chromatography
* Qualitative Analysis:- of individual components of a mixture
may be obtained by either of the following.
* by comparing the retention time or volume of the sample to the
standard, or
* by collecting the individual components as they emerge from the
chromatograph and subsequently identifying these compounds by
other method
* Quantitative Analysis- area under a single component elution
peak is proportional to the quantity of the detected component.
27. Miscellaneous applications of GC
The detection of steroid drugs used by athletes in sport competition
and steroid administration to animals in traces being carried by GLC.
In analysis of food, the separation of and identification of lipids,
proteins, carbohydrates, flavors, colorants and texture modifiers as
well as vitamins, steroids, drugs and pesticides trace elements being
analyzed by GC.
It is possible to analyzed dairy products by GLC for aldehyde and
ketones (for rancidity), fatty acid and milk sugar.
butter is analyzed for the butter fat content and for added color and
flavour.
It is possible to use pyrolysis GC for separation of and identification
of volatile material like plastics, natural and synthetic polymer, paints
and microbiological samples.
It may be use for analysis of pollutant, inorganic compounds etc.