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FORTIFICATION OF RICE
(Folic acid and Vitamin B12)
Aakriti
MSC 1130
Introduction
• Food fortification: The deliberate addition of
one or more micronutrients to particular
foods.
Types of fortification
Mass fortification
(general public)
Targeted fortification
(specific population)
Market-driven
Fortification
(business oriented
initiative)
Rice fortification
• Definition: Enrichment of rice with essential
micronutrients post harvesting to increase its
nutritional value.
• Why rice fortification??
Perfect food vehicle for fortification (staple
food).
Restores the nutritious components in milled
products.
Improves daily intake of essential nutrients.
Rice fortification terminology
• Fortificant: Source of each micronutrient.
• Fortificant mix: Blend of all the fortificants.
• Micronutrient premix: Fortificant mix ready for
use directly in rice fortification.
• Rice premix: Rice grains highly fortified with the
fortificant mix.
• Retail rice: Polished rice that is packaged at the
rice mills.
• Fortified rice: Retail rice combined with
micronutrient premix or the rice premix.
Source: Nina et al., 2012
Technologies for rice fortification
• Dusting
• Coating
• Extrusion
Cold
Hot
Dusting
• Fortification method: Dusting rice grain with
micronutrient premix powder (electrostatic
forces).
• Characteristics: Micronutrient premix on
kernel’s surface lost with rinsing and washing.
• Examples: Iron, vitamin B1, vitamin B3, folic
acid.
Source: Nina et al., 2012
Coating
• Fortification method: Fortification mix and
liquid made of waxes and gums is sprayed
onto the surface of rice kernels in several
layers.
• Characteristics: Distinctive color, smell and
taste.
• Examples: Vitamin B1, vitamin B3, vitamin B12,
vitamin E, folic acid, selenium, zinc.
Source: Nina et al., 2012
Cold extrusiuon
Dough
(Rice flour + fortificant mix + water)
Extrusion
(Pasta press, below 70ºC)
Cutting
Drying
Characteristics:
opaque, slightly off
color, easier to
distinguish from
rice kernels
Examples: Vitamin
B1, vitamin B3,
vitamin B12,
vitamin E, folic
acid, selenium, zinc
Source: Nina et al., 2012
Hot extrusion
Dough
(Rice flour + fortificant mix + water + steam)
Extrusion
[single or twin screw extruder, (70-110)º C]
Cutting
Drying
Characteristics: Similar
appearance as regular rice
(shine and transparency)
Examples: Vitamin B1, vitamin
B3, folic acid, zinc
Source: Nina et al., 2012
Quality assurance of fortified rice
• Grain shape (rice like or not)
• Appearance (off color or not)
• Odor (nil)
• Grain strength
• Moisture content (12%)
• KETT value (whiteness)
• Cooking quality (90% after rinsing & 80% after cooking)
• Weight
• Broken content (20%- grade #5, 15%- grade #3, 7%- grade #2)
• Microbial(Salmonella, E. coli, S. aureus, Yeast & mold,
Aflatoxin B1, B2, G1, G2)
• Coefficient of variation (<15%)
Source: Rice fortification toolkit, 2015
Folate fortification
• Essential coenzyme
• Deficiency causes: Megaloblastic anemia, birth defects
(neural tube defects), CVD, certain cancers
• Polished rice: 3-14 μg/100g (poor source)
• General range of fortification (USFDA): 100-300
μg/100g
• Criteria for a good folic acid fortification:
 Prevent the leaching of vitamins during washing and
cooking
 Mask the yellow color of folic acid in fortified rice
Folate fortification techniques
• Coating: Spraying first with folic acid solution
followed by coating with ethyl cellulose (Ashok et
al., 2003)
• Parboiling: 70ºC soaking for 3 h with a fortificant
concentration of 1.2 g/300g brown rice showed
90% of folate retention {20-300 μg/g}(Karrie et
al., 2012)
• Biofortification: Over expressing two Arabidopsis
thaliana genes leads to rise in folate retention by
15-100 folds (Storozhenko et al., 2007)
Vitamin B12 fortification
• Cofactor (synthesis of methionine)
• Metabolic role is closely related to folate
• Deficiency causes: Megaloblastic anemia, neurological
disorders
• No effect on color and is neutral in taste
• Soaking: 90ºC for 15 min (Karathanos et al., 2011)
• Spraying: At 35ºC followed by oven drying (Karathanos
et al., 2011)
• For physiological reasons, it is highly recommended to
fortify rice with both folate and vitamin B12.
Advantages
• Retention of micronutrients.
• Improves nutritional status of population.
• Does not require any change in the existing
food pattern.
• Minimal risk of chronic toxicity.
• Similar to regular rice.
Limitations
• High cost of fortification.
• Cannot be consumed by all members of
targeted population.
• Often fail to reach the poorest segment of
population.
• Technological issues.
• Lack of knowledge.
Conclusion
• Rice fortification can make a significant contribution
to addressing micronutrient deficiencies.
• A broad multi-sectoral strategy should be employed
to improve micronutrient health.
• Current technology can produce fortified rice that is
safe, looks and tastes similar to regular rice.
• Rice fortification has the greatest potential for public
health impact when it is mandated and well
regulated.
• As rice fortification is scaled up, it will achieve
economies of scale which will reduce costs.
References
• Ashok et al., 2003. Food Research
International.
• Karathanos et al., 2011. LWT - Food Science
and Technology.
• Karrie et al., 2012. Journal of Cereal Science.
• Nina et al.,2015. Food and Nutrition Bulletin.
• Rice fortification toolkit, 2015
• Storozhenko et al., 2007. Nat Biotechnol.
THANKS!!

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Fortification of rice

  • 1. FORTIFICATION OF RICE (Folic acid and Vitamin B12) Aakriti MSC 1130
  • 2. Introduction • Food fortification: The deliberate addition of one or more micronutrients to particular foods. Types of fortification Mass fortification (general public) Targeted fortification (specific population) Market-driven Fortification (business oriented initiative)
  • 3. Rice fortification • Definition: Enrichment of rice with essential micronutrients post harvesting to increase its nutritional value. • Why rice fortification?? Perfect food vehicle for fortification (staple food). Restores the nutritious components in milled products. Improves daily intake of essential nutrients.
  • 4. Rice fortification terminology • Fortificant: Source of each micronutrient. • Fortificant mix: Blend of all the fortificants. • Micronutrient premix: Fortificant mix ready for use directly in rice fortification. • Rice premix: Rice grains highly fortified with the fortificant mix. • Retail rice: Polished rice that is packaged at the rice mills. • Fortified rice: Retail rice combined with micronutrient premix or the rice premix. Source: Nina et al., 2012
  • 5. Technologies for rice fortification • Dusting • Coating • Extrusion Cold Hot
  • 6. Dusting • Fortification method: Dusting rice grain with micronutrient premix powder (electrostatic forces). • Characteristics: Micronutrient premix on kernel’s surface lost with rinsing and washing. • Examples: Iron, vitamin B1, vitamin B3, folic acid. Source: Nina et al., 2012
  • 7. Coating • Fortification method: Fortification mix and liquid made of waxes and gums is sprayed onto the surface of rice kernels in several layers. • Characteristics: Distinctive color, smell and taste. • Examples: Vitamin B1, vitamin B3, vitamin B12, vitamin E, folic acid, selenium, zinc. Source: Nina et al., 2012
  • 8. Cold extrusiuon Dough (Rice flour + fortificant mix + water) Extrusion (Pasta press, below 70ºC) Cutting Drying Characteristics: opaque, slightly off color, easier to distinguish from rice kernels Examples: Vitamin B1, vitamin B3, vitamin B12, vitamin E, folic acid, selenium, zinc Source: Nina et al., 2012
  • 9. Hot extrusion Dough (Rice flour + fortificant mix + water + steam) Extrusion [single or twin screw extruder, (70-110)º C] Cutting Drying Characteristics: Similar appearance as regular rice (shine and transparency) Examples: Vitamin B1, vitamin B3, folic acid, zinc Source: Nina et al., 2012
  • 10. Quality assurance of fortified rice • Grain shape (rice like or not) • Appearance (off color or not) • Odor (nil) • Grain strength • Moisture content (12%) • KETT value (whiteness) • Cooking quality (90% after rinsing & 80% after cooking) • Weight • Broken content (20%- grade #5, 15%- grade #3, 7%- grade #2) • Microbial(Salmonella, E. coli, S. aureus, Yeast & mold, Aflatoxin B1, B2, G1, G2) • Coefficient of variation (<15%) Source: Rice fortification toolkit, 2015
  • 11. Folate fortification • Essential coenzyme • Deficiency causes: Megaloblastic anemia, birth defects (neural tube defects), CVD, certain cancers • Polished rice: 3-14 μg/100g (poor source) • General range of fortification (USFDA): 100-300 μg/100g • Criteria for a good folic acid fortification:  Prevent the leaching of vitamins during washing and cooking  Mask the yellow color of folic acid in fortified rice
  • 12. Folate fortification techniques • Coating: Spraying first with folic acid solution followed by coating with ethyl cellulose (Ashok et al., 2003) • Parboiling: 70ºC soaking for 3 h with a fortificant concentration of 1.2 g/300g brown rice showed 90% of folate retention {20-300 μg/g}(Karrie et al., 2012) • Biofortification: Over expressing two Arabidopsis thaliana genes leads to rise in folate retention by 15-100 folds (Storozhenko et al., 2007)
  • 13. Vitamin B12 fortification • Cofactor (synthesis of methionine) • Metabolic role is closely related to folate • Deficiency causes: Megaloblastic anemia, neurological disorders • No effect on color and is neutral in taste • Soaking: 90ºC for 15 min (Karathanos et al., 2011) • Spraying: At 35ºC followed by oven drying (Karathanos et al., 2011) • For physiological reasons, it is highly recommended to fortify rice with both folate and vitamin B12.
  • 14. Advantages • Retention of micronutrients. • Improves nutritional status of population. • Does not require any change in the existing food pattern. • Minimal risk of chronic toxicity. • Similar to regular rice.
  • 15. Limitations • High cost of fortification. • Cannot be consumed by all members of targeted population. • Often fail to reach the poorest segment of population. • Technological issues. • Lack of knowledge.
  • 16. Conclusion • Rice fortification can make a significant contribution to addressing micronutrient deficiencies. • A broad multi-sectoral strategy should be employed to improve micronutrient health. • Current technology can produce fortified rice that is safe, looks and tastes similar to regular rice. • Rice fortification has the greatest potential for public health impact when it is mandated and well regulated. • As rice fortification is scaled up, it will achieve economies of scale which will reduce costs.
  • 17. References • Ashok et al., 2003. Food Research International. • Karathanos et al., 2011. LWT - Food Science and Technology. • Karrie et al., 2012. Journal of Cereal Science. • Nina et al.,2015. Food and Nutrition Bulletin. • Rice fortification toolkit, 2015 • Storozhenko et al., 2007. Nat Biotechnol.