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• Ateeq Ur Rahman
• Abdul Rauf
• Adil Zafar
• Junaid Ihsan
• Mohib Ullah
• Bakhtiar Ali
• Umar Hasnain
Food Enzymes and their Use in Industries
Presented To: Dr. MUDASSIR
Food Biotechnology
What is Enzyme?
• A substance produced by a living
organism that acts as a catalyst to bring
about a specific biochemical reaction.
Enzymes
• 2000 BC Egyptian and Sumerains developed fermentation for use in
brewing,
bread making and cheese making.
• 800 BC Calves’ stomach and the enzyme, chymosin, used for cheese
making.
• 1878 Yeast cells which cause fermentation were identified and the term
‘enzyme’ has first named meaning ‘in yeast’ in Greek.
• 1926 Enzymes were first shown to be protein.
• 1980 Enzyme preparation was developed to improved the digestibility
and
nutrient availability for animal feed.
5
Food Enzyme
• Enzyme use to digest the food is called food enzyme
• After food reaches the small intestine, the
enzyme pancreatin further digests protein, carbohydrates and fats
in the small intestine.
• Pancreatin also helps to support the pancreas.
• Alpha amylase digests starches.
• Bile salt makes fats soluble, preparing them for further digestion
by lipase.
Food Processing
Food processing combines raw food ingredients to
produce marketable food products that can be easily
prepared and served to the consumer.
Food processing
Advantages of Food Enzymes:
The two main advantages of food enzymes are:
Cut the costs
• Less time consumption
• Cut the Cost
Dairy
production
Brewing Baking Wine and
fruit juice
Meat
Rennet
Lactase
Protease
Catalases
β-Glucanase
α-Amylase
Protease
Amylogluco-
sidase
Maltogenic
amylase
Glucose oxidase
Pentosenase
Pectinase
β-Glucanase
Protease
Papain
Enzymes used in food industry 9
Enzyme Used in Food Industries
1. Dairy Industry:
Rennet
Lactase
Protease
Catalase
Rennet
• Extracted from the forth stomach of young calves
• Contains enzymes that cause milk to become cheese
• It separates solid curd and liquid whey
• Different animal rennet are used for different cheese
• Most common vegetable rennet is “thistle”
Lactase:
• Present in the brush border of the small intestine
• Artificially extracted from yeast
• Required for the digestion of whole milk
• Used in production of lactose free milk
• Also used in production of ice cream and sweetened
flavoured and condensed milks
Catalase:
• Produced from bovine livers or microbial sources
• Breaks down hydrogen peroxide to water and
molecular oxygen
• Along with glucose oxidase it is used in treating food
wrappers to prevent oxidation
• Also used to remove traces of hydrogen peroxide in
the process of cold sterilization
Protease:
• Widely distributed in biological world
• Hydrolyses the specific peptide bond to generate para-
k-casein and macro peptides in production of cheese
• Results in better flavour to the cheese and also in
desired texture
2. Baking Industry
Maltogenic amylase
Glucose oxidase
Pentosanase
Maltogenic Amylase
• Flour supplement
• It has anti staling effect
• It modifies starch while most of the starch starts to
gelatinise
• Resulting starch granules become more flexible during
storage.
Glucose Oxidase:
• Oxidizes glucose and produce gluconic acid and
hydrogen peroxide
• H2O2 is strong oxidizing agent that strengthens the
disulfide and non-disulfide cross-links in gluten
• Good working conditions help proper function of
bakery system.
Pentosanase:
• Exact mechanism is not yet discovered
• Improves dough machinability, yielding a more
flexible, easier-to-handle dough.
• The dough is more stable and gives better oven
spring during baking
3. Brewing indestry
β – Glucanase
α - Amylase
Protease
Amyloglucosidase
Inside the barley kernel
Protease
• Peptidase works to provide the wort with amino acid
nutrients that will be used by the yeast.
• Protease works to break up the larger proteins which
enhances the head retention of beer and reduces haze.
• In fully modified malts, these enzymes have done their
work during the malting process.
β-glucanase
• Beta- Glucanase represents a group of
carbohydrate enzymes which break down glycosidic
bonds within beta-glucan
• Aids in filtration after mashing and brewing
α- Amylase
• Converts starch to dextrins in producing corn syrup
• Solubilizes carbohydrates found in barley and
other cereals used in brewing
• Decreases the time required for mashing
Amyloglucosidase
• It is not as thermostable as Termamyl
• Optimum pH is 6.5
• Long incubation result in caramelisation of the
saccharides – resulting in product loss .
4. Meat Industry:
Protease
Papain
Protease
• Cleaves the bond that hold the amino acids
together.
• As the enzymes break apart proteins, which
disrupts or loosens muscle fibres and
tenderizes it.
Papain
• Found in papaya
• 95% of meat tenderizers available in grocery
store are made from papain
• It is extracted from latex in papaya fruits
• These enzymes are purified and sold in powder
or liquid form.
5. Fruit Industry
• Cellulase
• Hemicellulase
• Protopectin
Cellulase & Hemicellulase
• Enzyme that include CELLULASE and
HEMICELLULASE activity can break down the cell wall
and increase the over-all juice production by 5 – 10
%.
6. Vegetable Industry:
• Three enzymes important in vegetable industry
• Cellulase:
• Hydrolyze cellulase
• Pectinase:
• Hydrolyze pectin
Amyloglucosidase
• is an extracellular enzyme that converts starch to
dextrin's and glucose. The enzyme is used in the
starch-processing industry for the commercial
production of D-glucose from corn syrups.
7. Juice Industry
Pectinase
β – Glucanase
Pectinase:
• Prevents pectin from forming haze and hence to get
clear solution.
• Additionally its is used for extraction of colour and
juice from fresh fruit
• It breaks down pectin and releases methanol, which
is high amounts hazardous.
β- Glucanases
• It accelerates all biological mechanisms linked to
maturation on lees
• Reduces maturation duration
• Improves clarification and filtration, and improves
the action of fining agents
Conculsion
•The enzyme-based processes are advantageous as
they can be carried out at moderate reaction
conditions with greater specificity in contrast to
chemical-based processes.
•Market for enzymes in industrial processes is fast
growing.
THANKS
raufafridi2258@gmail.com
03139513193

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Food rauf

  • 1.
  • 2. Group Members • Ateeq Ur Rahman • Abdul Rauf • Adil Zafar • Junaid Ihsan • Mohib Ullah • Bakhtiar Ali • Umar Hasnain
  • 3. Food Enzymes and their Use in Industries Presented To: Dr. MUDASSIR Food Biotechnology
  • 4. What is Enzyme? • A substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction.
  • 5. Enzymes • 2000 BC Egyptian and Sumerains developed fermentation for use in brewing, bread making and cheese making. • 800 BC Calves’ stomach and the enzyme, chymosin, used for cheese making. • 1878 Yeast cells which cause fermentation were identified and the term ‘enzyme’ has first named meaning ‘in yeast’ in Greek. • 1926 Enzymes were first shown to be protein. • 1980 Enzyme preparation was developed to improved the digestibility and nutrient availability for animal feed. 5
  • 6. Food Enzyme • Enzyme use to digest the food is called food enzyme • After food reaches the small intestine, the enzyme pancreatin further digests protein, carbohydrates and fats in the small intestine. • Pancreatin also helps to support the pancreas. • Alpha amylase digests starches. • Bile salt makes fats soluble, preparing them for further digestion by lipase.
  • 7. Food Processing Food processing combines raw food ingredients to produce marketable food products that can be easily prepared and served to the consumer. Food processing
  • 8. Advantages of Food Enzymes: The two main advantages of food enzymes are: Cut the costs • Less time consumption • Cut the Cost
  • 9. Dairy production Brewing Baking Wine and fruit juice Meat Rennet Lactase Protease Catalases β-Glucanase α-Amylase Protease Amylogluco- sidase Maltogenic amylase Glucose oxidase Pentosenase Pectinase β-Glucanase Protease Papain Enzymes used in food industry 9 Enzyme Used in Food Industries
  • 11. Rennet • Extracted from the forth stomach of young calves • Contains enzymes that cause milk to become cheese • It separates solid curd and liquid whey • Different animal rennet are used for different cheese • Most common vegetable rennet is “thistle”
  • 12. Lactase: • Present in the brush border of the small intestine • Artificially extracted from yeast • Required for the digestion of whole milk • Used in production of lactose free milk • Also used in production of ice cream and sweetened flavoured and condensed milks
  • 13. Catalase: • Produced from bovine livers or microbial sources • Breaks down hydrogen peroxide to water and molecular oxygen • Along with glucose oxidase it is used in treating food wrappers to prevent oxidation • Also used to remove traces of hydrogen peroxide in the process of cold sterilization
  • 14. Protease: • Widely distributed in biological world • Hydrolyses the specific peptide bond to generate para- k-casein and macro peptides in production of cheese • Results in better flavour to the cheese and also in desired texture
  • 15. 2. Baking Industry Maltogenic amylase Glucose oxidase Pentosanase
  • 16. Maltogenic Amylase • Flour supplement • It has anti staling effect • It modifies starch while most of the starch starts to gelatinise • Resulting starch granules become more flexible during storage.
  • 17. Glucose Oxidase: • Oxidizes glucose and produce gluconic acid and hydrogen peroxide • H2O2 is strong oxidizing agent that strengthens the disulfide and non-disulfide cross-links in gluten • Good working conditions help proper function of bakery system.
  • 18. Pentosanase: • Exact mechanism is not yet discovered • Improves dough machinability, yielding a more flexible, easier-to-handle dough. • The dough is more stable and gives better oven spring during baking
  • 19. 3. Brewing indestry β – Glucanase α - Amylase Protease Amyloglucosidase
  • 21. Protease • Peptidase works to provide the wort with amino acid nutrients that will be used by the yeast. • Protease works to break up the larger proteins which enhances the head retention of beer and reduces haze. • In fully modified malts, these enzymes have done their work during the malting process.
  • 22. β-glucanase • Beta- Glucanase represents a group of carbohydrate enzymes which break down glycosidic bonds within beta-glucan • Aids in filtration after mashing and brewing
  • 23. α- Amylase • Converts starch to dextrins in producing corn syrup • Solubilizes carbohydrates found in barley and other cereals used in brewing • Decreases the time required for mashing
  • 24. Amyloglucosidase • It is not as thermostable as Termamyl • Optimum pH is 6.5 • Long incubation result in caramelisation of the saccharides – resulting in product loss .
  • 26. Protease • Cleaves the bond that hold the amino acids together. • As the enzymes break apart proteins, which disrupts or loosens muscle fibres and tenderizes it.
  • 27. Papain • Found in papaya • 95% of meat tenderizers available in grocery store are made from papain • It is extracted from latex in papaya fruits • These enzymes are purified and sold in powder or liquid form.
  • 28. 5. Fruit Industry • Cellulase • Hemicellulase • Protopectin
  • 29. Cellulase & Hemicellulase • Enzyme that include CELLULASE and HEMICELLULASE activity can break down the cell wall and increase the over-all juice production by 5 – 10 %.
  • 30. 6. Vegetable Industry: • Three enzymes important in vegetable industry • Cellulase: • Hydrolyze cellulase • Pectinase: • Hydrolyze pectin
  • 31. Amyloglucosidase • is an extracellular enzyme that converts starch to dextrin's and glucose. The enzyme is used in the starch-processing industry for the commercial production of D-glucose from corn syrups.
  • 33. Pectinase: • Prevents pectin from forming haze and hence to get clear solution. • Additionally its is used for extraction of colour and juice from fresh fruit • It breaks down pectin and releases methanol, which is high amounts hazardous.
  • 34. β- Glucanases • It accelerates all biological mechanisms linked to maturation on lees • Reduces maturation duration • Improves clarification and filtration, and improves the action of fining agents
  • 35. Conculsion •The enzyme-based processes are advantageous as they can be carried out at moderate reaction conditions with greater specificity in contrast to chemical-based processes. •Market for enzymes in industrial processes is fast growing.