This document discusses various enzymes used in food processing and their functions. It begins by listing common food processing enzymes such as amylase, lipase, protease, and cellulase. It then provides details on the role of specific amylases, cellulase, catalase, glucanases, glucose oxidase, hemicellulase, lipase, pectinase, rennet, tannase, xylanase, and their uses in food industries like baking, brewing, dairy, and fruit juice processing. It concludes with a brief overview of how enzymes like amylases and glucanases are used in beer manufacturing to break down barley and produce fermentable sugars.
In the field of biotechnology there are many industrial applications that result in biotech products that we use everyday at home. Some of these are food science applications that utilize enzymes to produce or make improvements in the quality of different foods. In the dairy industry, some enzymes are required for the production of cheeses, yogurt and other dairy products, while others are used in a more specialized fashion to improve texture or flavour.
Isolation and Purification of Enzymes
Enzymes are unstable molecules with a definite physico chemical organization. Even a slight change in this organization reduces the activity of enzyme and sometimes the enzyme is totally inactivated.
Therefore, the enzymes have to be isolated under controlled conditions of pH, ionic strength and temperature. Since they are proteinaceous in nature, standard extraction and purification procedures for enzymes are the same as those used for proteins except that the activity of the enzyme is assayed at each of the following four steps of extraction and purification.
Purification of Enzymes - Enzyme purification involves three steps, electrophoresis. These three techniques described in the following text
1.Dialysis
2.Chromatography.
The Pectinases are a group of related enzymes capable of degrading pectin.
Also known as pectic enzymes or pectolytic enzymes.
Pectinases are synthesized by microorganisms which used in industrial production.
They are inexpensive and environmentally friendly.
When compared with chemical methods they are
More specific
Less aggressive
Generates low toxicity
In the field of biotechnology there are many industrial applications that result in biotech products that we use everyday at home. Some of these are food science applications that utilize enzymes to produce or make improvements in the quality of different foods. In the dairy industry, some enzymes are required for the production of cheeses, yogurt and other dairy products, while others are used in a more specialized fashion to improve texture or flavour.
Isolation and Purification of Enzymes
Enzymes are unstable molecules with a definite physico chemical organization. Even a slight change in this organization reduces the activity of enzyme and sometimes the enzyme is totally inactivated.
Therefore, the enzymes have to be isolated under controlled conditions of pH, ionic strength and temperature. Since they are proteinaceous in nature, standard extraction and purification procedures for enzymes are the same as those used for proteins except that the activity of the enzyme is assayed at each of the following four steps of extraction and purification.
Purification of Enzymes - Enzyme purification involves three steps, electrophoresis. These three techniques described in the following text
1.Dialysis
2.Chromatography.
The Pectinases are a group of related enzymes capable of degrading pectin.
Also known as pectic enzymes or pectolytic enzymes.
Pectinases are synthesized by microorganisms which used in industrial production.
They are inexpensive and environmentally friendly.
When compared with chemical methods they are
More specific
Less aggressive
Generates low toxicity
Fermentation is a natural process, occurs when microorganisms consume susceptible organic substrate as part of their own metabolic processes. Also referred to the bulk growth of microorganisms in a growth medium with the goal of producing a specific chemical product. The science of fermentation is called zymology. It mainly takes place when the electron transport chain unusable. Extract energy but do not consume O₂ or change the concentration of NAD⁺ or NADH.
The integration of enzymes in food and feed processes is a well-established approach; however there are clear evidences that dedicated research efforts are consistently being made to make the applications of biological agents more effective as well as diversified.
Various techniques have been employed such as rDNA technology and protein engineering (site-directed mutagenesis and random mutation) for the design of new/improved biocatalysts
Advances in molecular biology, evolution- ary protein engineering expertise, the (bio) computational tools, and the implementation of high-throughput meth- odologies enabling the efficient and timely screening/ characterization of the biocatalysts.
There needs to be continue efforts in the direction to have more diverse, versatile and robust enzymes to be applied in food technology
Upon the evolution brought about in the fermentation technology resulted out into various methodologies for optimization of the product yield by economical consumption of the substrates. Eventually, these ventures led for the development of technologies classified into as Submerged and Solid State technologies and the latter one being the concept of interest whose detailed view will be provided in the following presentation
Wild strains of microorganisms produce low quantities of commercially important metabolites.
Therefore we need genetic improvement to produce high quantities of metabolites/products.
MICROBIAL METABOLITES AS FLAVOURING AGENTSsarabjit777
Flavour is the sensory impression of food or other substance determined by chemical sense of taste and smell. Flavour can be characterized as plants, amimal, microbial, enzymatic. . Microbial bioprocess has advantageous over plant cell culture such as high yield, low cost, independent on seasonal variation.
A report on the development of Fermentation. Includes B.C. to A.D. coverage of how fermentation was utilized and also various scientists study on discovering the scientific process behind the process. Also included is the kinds of fermenters and its development over the years.
Fermentation is a natural process, occurs when microorganisms consume susceptible organic substrate as part of their own metabolic processes. Also referred to the bulk growth of microorganisms in a growth medium with the goal of producing a specific chemical product. The science of fermentation is called zymology. It mainly takes place when the electron transport chain unusable. Extract energy but do not consume O₂ or change the concentration of NAD⁺ or NADH.
The integration of enzymes in food and feed processes is a well-established approach; however there are clear evidences that dedicated research efforts are consistently being made to make the applications of biological agents more effective as well as diversified.
Various techniques have been employed such as rDNA technology and protein engineering (site-directed mutagenesis and random mutation) for the design of new/improved biocatalysts
Advances in molecular biology, evolution- ary protein engineering expertise, the (bio) computational tools, and the implementation of high-throughput meth- odologies enabling the efficient and timely screening/ characterization of the biocatalysts.
There needs to be continue efforts in the direction to have more diverse, versatile and robust enzymes to be applied in food technology
Upon the evolution brought about in the fermentation technology resulted out into various methodologies for optimization of the product yield by economical consumption of the substrates. Eventually, these ventures led for the development of technologies classified into as Submerged and Solid State technologies and the latter one being the concept of interest whose detailed view will be provided in the following presentation
Wild strains of microorganisms produce low quantities of commercially important metabolites.
Therefore we need genetic improvement to produce high quantities of metabolites/products.
MICROBIAL METABOLITES AS FLAVOURING AGENTSsarabjit777
Flavour is the sensory impression of food or other substance determined by chemical sense of taste and smell. Flavour can be characterized as plants, amimal, microbial, enzymatic. . Microbial bioprocess has advantageous over plant cell culture such as high yield, low cost, independent on seasonal variation.
A report on the development of Fermentation. Includes B.C. to A.D. coverage of how fermentation was utilized and also various scientists study on discovering the scientific process behind the process. Also included is the kinds of fermenters and its development over the years.
Enzymes are a biological substance that accelerates the rate of various biochemical reactions in a living organism without being used up in the reaction. Their role in food processing has also been recognized for many centuries. Even before this knowledge about enzymes, they have been used in a number of processes such as the tenderization of meat using papaya leaves, soy sauce preparation, curd or cheese making, baking, brewing, etc. From animals to plants to microbial sources, enzymes may be extracted from any living organisms. Of the hundred or so enzymes being used in industries, more than half are of microbial origin. In the food industry, microbial enzymes have been extensively used to increase the diversity, variety, and quality of food. Microorganisms as an enzyme source are always preferred over other sources as large amounts of enzymes can be produced from them in a controlled manner that is also faster and cheaper. Moreover, the minimum of potentially harmful content is present in microbial enzymes in comparison to those of plants and animals. This chapter includes microbial enzymes used in food processing and the food industry, their physicochemical and biological properties, recent developments, and future prospects.
Roti Bank Hyderabad: A Beacon of Hope and NourishmentRoti Bank
One of the top cities of India, Hyderabad is the capital of Telangana and home to some of the biggest companies. But the other aspect of the city is a huge chunk of population that is even deprived of the food and shelter. There are many people in Hyderabad that are not having access to
Key Features of The Italian Restaurants.pdfmenafilo317
Filomena, a renowned Italian restaurant, is renowned for its authentic cuisine, warm environment, and exceptional service. Recognized for its homemade pasta, traditional dishes, and extensive wine selection, we provide a true taste of Italy. Its commitment to quality ingredients and classic recipes has made it a adored dining destination for Italian food enthusiasts.
At Taste Of Middle East, we believe that food is not just about satisfying hunger, it's about experiencing different cultures and traditions. Our restaurant concept is based on selecting famous dishes from Iran, Turkey, Afghanistan, and other Arabic countries to give our customers an authentic taste of the Middle East
Piccola Cucina is regarded as the best restaurant in Brooklyn and as the best Italian restaurant in NYC. We offer authentic Italian cuisine with a Sicilian touch that elevates the entire fine dining experience. We’re the first result when someone searches for where to eat in Brooklyn or the best restaurant near me.
Ang Chong Yi Navigating Singaporean Flavors: A Journey from Cultural Heritage...Ang Chong Yi
In the heart of Singapore, where tradition meets modernity, He embarks on a culinary adventure that transcends borders. His mission? Ang Chong Yi Exploring the Cultural Heritage and Identity in Singaporean Cuisine. To explore the rich tapestry of flavours that define Singaporean cuisine while embracing innovative plant-based approaches. Join us as we follow his footsteps through bustling markets, hidden hawker stalls, and vibrant street corners.
Ang Chong Yi Navigating Singaporean Flavors: A Journey from Cultural Heritage...
Enzymes
1. Enzymes
A.Reni
Assistant Professor
Department of Food Processing and Preservation Technology
Faculty of Engineering
Avinashilingam Institute for Home Science and Higher Education for Women
Coimbatore-641108
2. Enzymes can speed reactions by more than 1010 times—they
can function uniquely to control process time, enhance
flavour, improve texture, extend shelf life and decrease the
use of chemical food additives.
4. AMYLASES:
Amylases act on starch (amylose and amylopectin).
They split starch into dextrins and sugars by cleaving the a-
1,4 glycosidic linkages in the interior of the starch chain.
Amylases can be derived from bacteria and fungi.
They play a major role in the, food & beverages (baking),
brewing, starch, sugar & alcohol industries.
5. ALPHA-AMYLASES:
Fungal alpha-amylase: For detoxing and saccharifying
starch.
Bacterial alpha-amylase: For starch liquefaction at
temperatures up to 90º C.
Thermostable bacterial alpha-amylase: For starch
liquefaction at temperatures above 90º C.
6. AMYLOGLUCOSIDASE:
Also called glucoamylase, this enzyme acts on starch,
dextrins and sugars by cleaving the a-1, 4 glycosidic
linkages releasing stepwise from the end of the chain.
It is widely used in the manufacture of glucose and for
conversion of carbohydrates to fermentable sugars.
They play a major role in starch sugar & alcohol
industries.
7. GLUCOAMYLASES:
Fungal Glucoamylase: is capable of hydrolyzing glucosidic
linkages of starch.
Glucoamylase and pullulanase blend: for higher glucose yields
from starch
8. CELLULASE
• Cellulase acts on cellulose molecules by hydrolysing the b-1,4
glycosidic linkages.
• It largely produces cellobiose, which can ultimately yield glucose
units, depending on the characteristic of the enzyme.
• Cellulases find wide application in the food & beverages industries.
• Fungal cellulase: is primarily active on soluble forms of cellulase.
9. CATALASE:
Catalase is the enzyme that breaks down hydrogen peroxide
to water and molecular oxygen.
Catalase effectively removes the residual hydrogen peroxide,
ensuring that the fabric is peroxide-free and mainly used in
food industry and also in egg processing with other enzymes.
10. GLUCANASES:
Glucanase act on b-1,3 and b-1,4 bonds in b-D-glucans. B-
glucanases are of particular interest to the brewing industry,
where they act on the glucans that impede clarification of
wort and filtration of beer.
11. Beta-glucanase:
Thermostable bacterial beta-glucanase: for the hydrolysis of
cereal beta-glucan polysaccharides.
It reduce hazing and can also affect foaming during storage.
12. GLUCOSE OXIDASE:
Glucose and hexose oxidases catalyze reactions between
glucose and oxygen, producing gluconolactone and hydrogen
peroxide.
And,they are mainly used in baking-food industries
13. HEMICELLULASE:
Hemicellulases act on hemicellulose (also called pentosan), a polymer of
pentose sugars.
They are mainly used in the baking (food & beverages) industry to
improve the quality of dough, the softness of the crumb and volume.
Fungal hemicellulase: for the hydrolysis of galactomannan gums and
soluble cellulose
14. LIPASE:
Lipase breaks down natural lipids and oils to free fatty acids and
glycerol.
In otherwise,Lipase is usefull in baking and egg processing with other
enzymes.
Pancreatic Lipase: hydrolyzes insoluble fats and fatty acid esters to
yield monoglycerides, glycerides, glycerol and free fatty acids.
Microbial lipases: for fat hydrolysis and flavor development
15. PECTINASE:
Pectinases act on pectins and their derivatives and play a major role in
the food & beverages industry.
In fruit drinks,pectinase is a unique enzyme for the peeling of citrus
fruit.
It can be used to produce fresh fruit segments from oranges and
grapefruit and are less likely to damage fruits than conventional
cutting.
Pectinases cut pectin chains to lower viscosity,while glucose oxidase
removes dissolved oxygen, decrasing oxidative browning
16. PROTEASE
Proteases are enzymes that act on proteins and convert them to peptides
and free amino acids.
They play an important role in the food & beverages – baking
industries.
Depending on the application acid, neutral and alkaline proteases are
available.
17. RENNET:
Rennet, which is biologically prepared from the Mucor strain,
is used as a milk coagulant in the preparation of cheese.
The food & beverages industry, specifically the dairy
industry, has accepted microbial rennet as the next best thing
to natural rennet.
Micellium of Mucor miehei
18. TANNASE:
Tannase is an esterase-based formulation that hydrolyses the
acyl esters of tannins, making tannins more soluble at a lower
temperature and pH.
They are used in the production of instant tea (for solubility)
in the food & beverages industry.
19. XYLANASE:
Xylanases cleave chains of b-1,4-xylosidic linkages in Xylans.
They are mainly used in the food & beverages-
baking industries.
20. ENZYMES:
Enzyme in Beer Manufacturing - Beer is an
undistilled alcoholic bewerage produced from
fermentation of barley malt by yeast, especial1y S.
cerevisiae or S. carisbergensis
21. Barley malt is prepared by soaking the grains in
water, allowing them to germinate at 17°C, and then
drying them at 65°C once the appropriate stage of
germination is reached. For beer preparation the malt
is powdered, mixed with warm water at 70°C and
pH 5.0; this is called mashing.
22. α-Amylases and β-glucanase are used for increased
and rapid saccharification.
Neutral proteases are added to the wort to hydrolyse
proteins and to increase the fermentation rate in the
later stages.
23. Cellulases are used to digest barley glucans, particularly when
wheat is used as an adjunct.
Papain is added to the beer during the later post-fermentation
stages to hydrolyse the proteins and prevent the occurrence of
'chillhaze'.
Glucoamylase and/or fungal α-amylase are used during
fermentation to produce low calorie or 'light' beers.
24. A variety of distilled alcoholic drinks, e.g., whisky,
rum, vodka, etc., are prepared by fermentation of a
large number of substrates rich in carbohydrates.
Some of these substrates, e.g., molasses (rum), grapes
(brandy), etc., are rich in fermentable sugars; in such
cases, heat stable α-amylases from bacteria are used
for saccharification.