This document discusses the fine structure of genes. It defines a gene as a sequence of DNA that codes for a specific protein. In prokaryotes, genes are uninterrupted, while in eukaryotes, coding sequences called exons are separated by non-coding introns. Introns allow for alternative splicing, which increases protein diversity. The document outlines the key components of prokaryotic and eukaryotic genes, including exons, introns, promoters, terminators, and regulatory elements. It emphasizes the significance of introns in evolution and gene regulation.
Fine Structure of Gene- Biotechnology, Microbiology PPT DownloadEducation Bhaskar
Fine Structure of Gene- Biotechnology, Microbiology PPT, PDF
Download the presentation
SYNOPSIS
Introduction
History of gene
Definition of gene
Gene structure
Prokaryote gene
Eukaryote gene
Significance of introns.
References
Gene regulation in eukaryotes in a nutshell covering all the important stages of gene regulation in eukaryotes at transcriptional level, translation level and post-translational level.
RNA splicing, in molecular biology, is a form of RNA processing in which a newly made precursor messenger RNA transcript is transformed into a mature messenger RNA. During splicing, introns are removed and exons are joined together.
This presentation explains DNA transcription and RNA Processing.
It gives details about prokaryotic DNA transcription and eukaryotic DNA transcription. it also explains post-transcriptional modification both in prokaryotes and eukaryotes.
Fine Structure of Gene- Biotechnology, Microbiology PPT DownloadEducation Bhaskar
Fine Structure of Gene- Biotechnology, Microbiology PPT, PDF
Download the presentation
SYNOPSIS
Introduction
History of gene
Definition of gene
Gene structure
Prokaryote gene
Eukaryote gene
Significance of introns.
References
Gene regulation in eukaryotes in a nutshell covering all the important stages of gene regulation in eukaryotes at transcriptional level, translation level and post-translational level.
RNA splicing, in molecular biology, is a form of RNA processing in which a newly made precursor messenger RNA transcript is transformed into a mature messenger RNA. During splicing, introns are removed and exons are joined together.
This presentation explains DNA transcription and RNA Processing.
It gives details about prokaryotic DNA transcription and eukaryotic DNA transcription. it also explains post-transcriptional modification both in prokaryotes and eukaryotes.
The process of transcription is the first stage of gene expression resulting in the production of a primary RNA transcript from the DNA of a particular gene.
This step of gene expression which is followed by a number of post-transcriptional processes such as RNA splicing and translation.
These lead ultimately to the production of a functional protein and this process is highly regulated.
Both basal transcription and its regulation are dependent upon specific protein factors known as transcription factors.
These highly specific protein bind to the specific regulatory gene of DNA sequence and control the transcription process and regulate it.
For example- enzyme RNA polymerase catalyzes the chemical reaction that synthesize RNA, using the DNA gene as a template, the transcription factor control when, where, and how efficiency RNA polymerase function.
Play an important role in the normal development and routine of cellular function.
Translational proofreading and translational inhibitorsShritilekhaDash
Translation proofreading is often the final stage of a translation process.
Transcription creates a complementary RNA copy of a DNA sequence and translation is the subsequent process where RNA is used to synthesize the actual protein from amino acids. Inhibition of this translation step has the effect of blocking protein production and ultimately its function.
REGULATION OF
GENE EXPRESSION
IN PROKARYOTES & EUKARYOTES .
This presentation is enriched with lots of information of gene expression with many pictures so that anyone can understand gene expression easily.
Gene expression is the process by which the information encoded in a gene is used to direct the assembly of a protein molecule.
Gene expression is explored through a study of protein structure and function, transcription and translation, differentiation and stem cells.
It is the process by which information from a gene is used in the synthesis of a functional gene product.
These products are often proteins, but in non-protein coding genes such as ribosomal RNA (rRNA), transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.
The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea)
Regulation of gene expression:
Regulation of gene expression includes a wide range of mechanisms that are used by cells to increase or decrease the production of specific gene products (protein or RNA).
Gene regulation is essential for viruses, prokaryotes and eukaryotes as it increases the versatility and adaptability of an organism by allowing the cell to express protein when needed.
CLASSIFICATION OF GENE WITH RESPECT TO THEIR EXPRESSION:
Constitutive ( house keeping) genes:
Are expressed at a fixed rate, irrespective to the cell condition.
Their structure is simpler.
Controllable genes:
Are expressed only as needed. Their amount may increase or decrease with respect to their basal level in different condition.
Their structure is relatively complicated with some response elements.
TYPES OF REGULATION OF GENE:
positive & negative regulation.
Steps involving gene regulation of prokaryotes & eukaryotes.
Operon-structure,classification of mechanisms- lac operon,tryptophan operon ,
and many things related to gene expression.
This is a video slide so anyone can understand this topic easily by seeing pictures included in this slide.
Agrobacterium tumifaciens
Horizontal gene transfer
Interkingdom gene transfer
Virulence or Vir a b c d e f g genes
Crown gall disease
Regulation of vir genes
Relaxosome
Replication Introduction , DNA replicating Models , Meselson and Stahl Experiments , Circuler Model of DNA replication , Replication in Prokaryotes , Replication In Eukaryotes , Comparison Between Prokaryotes and Eukaryotes Replicaton and PCR (Polymerease Chain Reaction)
prokaryotic and Eukaryotic gene structure slide contains moving pictures and wobble hypothesis.
concepts explained through animation
Good transitions when downloaded or clipped
The process of transcription is the first stage of gene expression resulting in the production of a primary RNA transcript from the DNA of a particular gene.
This step of gene expression which is followed by a number of post-transcriptional processes such as RNA splicing and translation.
These lead ultimately to the production of a functional protein and this process is highly regulated.
Both basal transcription and its regulation are dependent upon specific protein factors known as transcription factors.
These highly specific protein bind to the specific regulatory gene of DNA sequence and control the transcription process and regulate it.
For example- enzyme RNA polymerase catalyzes the chemical reaction that synthesize RNA, using the DNA gene as a template, the transcription factor control when, where, and how efficiency RNA polymerase function.
Play an important role in the normal development and routine of cellular function.
Translational proofreading and translational inhibitorsShritilekhaDash
Translation proofreading is often the final stage of a translation process.
Transcription creates a complementary RNA copy of a DNA sequence and translation is the subsequent process where RNA is used to synthesize the actual protein from amino acids. Inhibition of this translation step has the effect of blocking protein production and ultimately its function.
REGULATION OF
GENE EXPRESSION
IN PROKARYOTES & EUKARYOTES .
This presentation is enriched with lots of information of gene expression with many pictures so that anyone can understand gene expression easily.
Gene expression is the process by which the information encoded in a gene is used to direct the assembly of a protein molecule.
Gene expression is explored through a study of protein structure and function, transcription and translation, differentiation and stem cells.
It is the process by which information from a gene is used in the synthesis of a functional gene product.
These products are often proteins, but in non-protein coding genes such as ribosomal RNA (rRNA), transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.
The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea)
Regulation of gene expression:
Regulation of gene expression includes a wide range of mechanisms that are used by cells to increase or decrease the production of specific gene products (protein or RNA).
Gene regulation is essential for viruses, prokaryotes and eukaryotes as it increases the versatility and adaptability of an organism by allowing the cell to express protein when needed.
CLASSIFICATION OF GENE WITH RESPECT TO THEIR EXPRESSION:
Constitutive ( house keeping) genes:
Are expressed at a fixed rate, irrespective to the cell condition.
Their structure is simpler.
Controllable genes:
Are expressed only as needed. Their amount may increase or decrease with respect to their basal level in different condition.
Their structure is relatively complicated with some response elements.
TYPES OF REGULATION OF GENE:
positive & negative regulation.
Steps involving gene regulation of prokaryotes & eukaryotes.
Operon-structure,classification of mechanisms- lac operon,tryptophan operon ,
and many things related to gene expression.
This is a video slide so anyone can understand this topic easily by seeing pictures included in this slide.
Agrobacterium tumifaciens
Horizontal gene transfer
Interkingdom gene transfer
Virulence or Vir a b c d e f g genes
Crown gall disease
Regulation of vir genes
Relaxosome
Replication Introduction , DNA replicating Models , Meselson and Stahl Experiments , Circuler Model of DNA replication , Replication in Prokaryotes , Replication In Eukaryotes , Comparison Between Prokaryotes and Eukaryotes Replicaton and PCR (Polymerease Chain Reaction)
prokaryotic and Eukaryotic gene structure slide contains moving pictures and wobble hypothesis.
concepts explained through animation
Good transitions when downloaded or clipped
Basics of Undergraduate/university fellows
Transcription is more complicated in eukaryotes than in prokaryotes because
eukaryotes possess three different classes of RNA polymerases and because of the
way in which transcripts are processed to their functional forms.
More proteins and transcription factors are involved in eukaryotic transcription.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
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2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
3. INTRODUCTION
A gene is a specific sequence of DNAcontaining
genetic information required to make a specific
protein
Prokaryotic gene is uninterrupted.
In Eukaryotic gene the coding sequences (exon)are
seprated by non-coding sequences called introns.
In complex eukaryotes, introns account for more
than 10 times as much DNA as exons.
4. History of genes:
The classical principles of genetics were deduced by
Gregor Mendel in 1865 on the basis of breeding
experiments with peas. He assumed that each trait is
determined by a pair of inherited ‘factors’ which are
now called gene.
Mendel’s work was rediscovered in 1900 by Hugo de
Vries,Correns and Tschermak
Wilhelm Johannsen coined the term ‘GENE’ in 1909.
William Bateson in 1905 coined the term genetics.
5. In 1972,Walter Fiers and his team determined the
sequence of a gene in bacteriophage MS2 coat protein.
Richard J Roberts and Phillip Sharp found the gene
can be split into segments making it possible that a
single gene might be coding for several proteins.
6. What is a gene?
•The gene is the
Functional unit of
Heredity.
•Each gene is a segment of DNA that give rise to a
protein product or RNA.
•A gene may exist in alternative forms called alleles.
•Chromosome in fact carry genes.
•Each chromosome consists of a linear array of genes.
7. PROKARYOTIC Gene structure
Genes based on their activity:
1.House keeping genes
2.Specific genes.
STRUCTURAL FEATURES:
Simple gene structure.
Small genomes(0.5 to 10 million bp).
Prokaryotic genes are collinear with their proteins.
a. CODING REGION
b. PROMOTER ELEMENTS
c. TERMINAL REGION OR TERMINATOR.
8. a. Coding region-
Starts with an initiator codon and ends with termination codon
No introns (uninterrupted).
Collinear to its mRNA.
9. b.Promoter elements-
The upstream elements from the start of the coding
region include promoter sequences.
•50 to 100 ntds upstream of the start codon-
transcriptional initiation site or START site.
•(any nucleotide present on the left is denoted by (-)symbol and
the region is called upstream element. E.g. -10,-20,-35 etc.
Start site symbolized by +1.
Any sequence to the right of the start is downstream elements
and numbered as +10,+35 etc.)
10. •At -65 to -60 activator elements. Activation of the polymerase.
•At -200 to -1000 enhancer sequence. Enhances transcription
by 100 to 200 folds.
•At -10 there is a sequence TATAATor PRIBNOW BOX.
•At -35 another consensus sequence TTGACA
These two are the most important promoter elements.
Recognized by transcription factors.
11. c. Terminal region of the gene-
Sequences for the termination of transcription.
It takes place by Rho dependent mode or Rho independent
mode.
12. Eukaryotic gene structure
Exons
Introns
Promoter sequences
Terminator sequences
Upstream sequences
Downstream sequences
Enhancers and silencers(upstream or downstream)
Signals
(Upstream sequence signal for addition of cap.
Downstream sequences signal for addition of poly A
tail.)
13. EXONS –coding sequence, transcribed and
translated. Coding for amino acids in the polypeptide
chain.
Vary in number ,sequence and length. A gene starts and
ends with exons.(5’ to 3’).
Some exon includes untranslated(UTR)region.
INTRONS- coding sequences are separated by non-
coding sequences called introns.
Any nucleotide sequence that are removed when the
primary transcript is processed to give the mature RNA
are called introns.
All introns share the base sequence GT in the 5’end
and AG in the 3’end.
Introns were 1st discovered in 1977 independently by
Phillip Sharp and Richard Roberts.
15. PROMOTERS- A promoter is a regulatory region of
DNA located upstream controlling gene expression.
1.Core promoter – transcription start site(-34)
Binding site for RNA polymerase.
General transcription factor binding sites.
2. Proximal promoter-contain primary regulatory element
Apprx. -250,specific transcription factor binding sites.
TATAbox or hogness box (-30 to -80)and
CAAT(upstream TATA) are two distinct sequences.
These together are responsible for binding of RNA
polymerase II which is responsible for transcription
16. UPSTREAM(5’END)- 5’UTR serve sevral functions
including mRNA transport and initiation of translation.
Signal for addition of cap(7 methyl guanisine) to the
5’end of the mRNA.
The cap facilitates the initiation of translation.
Stabilization of mRNA.
DOWNSTREAM(3’END)-3’UTR serves to add mRNA
stability and attachment site for poly-A-tail.
The translation termination codon TAA.
AATAA sequence signal for addition of poly Atail.
17.
18. TERMINATOR- recognized by RNA polymerase
as a signal to stop transcription
ENHANCER-enhances the transcription of a gene.
Upto few thousand bp upstream.
SILENCERS-reduce or shut off the expression of a
near by gene.
19. Significance of introns
Introns don't specify the synthesis of proteins
but have other important cellular activities.
Many introns encodes RNA’s that are major
regulators of gene expression.
Contain regulatory sequences that control
trancription and mRNA processing.
Introns allow exons to be joined in different
combinations(alternative splicing), resulting in the
synthesis of different proteins from the same gene.
Important role in evolution by facilitating
recombination between exons of different
genes(exon shuffling).
21. REFRENCES
oThe Cell (fifth edition) by Geoffrey M. Cooper and
Robert E. Hausman.
oGenes IX by Benjamin Lewin.
ohttp://faculty.ksu.edu.sa/77379/Documents
ohttps://en.wikipedia.org/wiki/Exon