This document summarizes research isolating and characterizing human fetal liver stem cells. The key steps involved isolating stem cells from human fetal liver tissue using markers like AFP, CK18, and albumin. The isolated stem cells were then characterized using various methods like immunocytochemistry, viability assays, RNA isolation, and RT-PCR. The goal was to isolate stem cells from human fetal liver that could be identified using the markers AFP, CK18, and albumin and further characterized.