EWMA 2014 - EP402 IN VITRO EVALUATION OF THE CAPACITY OF A, MONOFILAMENT DEBRIDER* TO REMOVE BIOFILM AND THE EFFICACY OF DIFFERENT WOUND DRESSINGS TO PREVENT BIOFILM RE-GROWTH
Intracanal medicaments /certified fixed orthodontic courses by Indian dental...Indian dental academy
Welcome to Indian Dental Academy
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and offering a wide range of dental certified courses in different formats.
Indian dental academy has a unique training program & curriculum that provides students with exceptional clinical skills and enabling them to return to their office with high level confidence and start treating patients
State of the art comprehensive training-Faculty of world wide repute &Very affordable.
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.
Indian Dental Academy: will be one of the most relevant and exciting training center with best faculty and flexible training programs for dental professionals who wish to advance in their dental practice,Offers certified courses in Dental implants,Orthodontics,Endodontics,Cosmetic Dentistry, Prosthetic Dentistry, Periodontics and General Dentistry.
Intracanal medicaments /certified fixed orthodontic courses by Indian dental...Indian dental academy
Welcome to Indian Dental Academy
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and offering a wide range of dental certified courses in different formats.
Indian dental academy has a unique training program & curriculum that provides students with exceptional clinical skills and enabling them to return to their office with high level confidence and start treating patients
State of the art comprehensive training-Faculty of world wide repute &Very affordable.
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.
Indian Dental Academy: will be one of the most relevant and exciting training center with best faculty and flexible training programs for dental professionals who wish to advance in their dental practice,Offers certified courses in Dental implants,Orthodontics,Endodontics,Cosmetic Dentistry, Prosthetic Dentistry, Periodontics and General Dentistry.
Classification of chemical antiplaque agents
1. FIRST GENERATION AGENTS
Poor substantivity and thus used 4-6 times daily.
Reduces plaque score by 20-50%
Examples:
Antibiotics like Penicillin, Erythromycin, Metronidazole
2. SECOND GENERATION AGENTS
Reduce plaque score by 70-90%
Used twice daily
Example: Bisbiguanides, Chlorhexidine, Alexidine
3. THIRD GENERATION AGENTS
Effective against specific periodontal pathogens
Example: Delmopinol
II. Vehicles for delivery of chemical agents
a. Toothpastes
b. Sprays
c. Irrigators
d. Chewing gums
e. Mouthwashes (Listerine, Chlorhexidine, Triclosan, Fluorides, Hydrogen peroxides, Povidone iodine)
This Slide contains the information about Techniques used for the identification of bacteria and also contains Various methods used to stain the bacteria.
Positive Staining
negative staining
Grams Staining
Acid Fast Staining
Ganoderma lucidum against Candida albicansCsikós Ilona
Ganoderma lucidum has been in use in Traditional Chinese Medicine for years. Literature supports the use of this Ganoderma lucidum as a medicinal mushroom for its antimicrobial, antiviral properties. Candida albicans is the most common oral fungus associated with oral candidial infections. More: http://www.ganodermacoffee.dxnnet.com
Classification of chemical antiplaque agents
1. FIRST GENERATION AGENTS
Poor substantivity and thus used 4-6 times daily.
Reduces plaque score by 20-50%
Examples:
Antibiotics like Penicillin, Erythromycin, Metronidazole
2. SECOND GENERATION AGENTS
Reduce plaque score by 70-90%
Used twice daily
Example: Bisbiguanides, Chlorhexidine, Alexidine
3. THIRD GENERATION AGENTS
Effective against specific periodontal pathogens
Example: Delmopinol
II. Vehicles for delivery of chemical agents
a. Toothpastes
b. Sprays
c. Irrigators
d. Chewing gums
e. Mouthwashes (Listerine, Chlorhexidine, Triclosan, Fluorides, Hydrogen peroxides, Povidone iodine)
This Slide contains the information about Techniques used for the identification of bacteria and also contains Various methods used to stain the bacteria.
Positive Staining
negative staining
Grams Staining
Acid Fast Staining
Ganoderma lucidum against Candida albicansCsikós Ilona
Ganoderma lucidum has been in use in Traditional Chinese Medicine for years. Literature supports the use of this Ganoderma lucidum as a medicinal mushroom for its antimicrobial, antiviral properties. Candida albicans is the most common oral fungus associated with oral candidial infections. More: http://www.ganodermacoffee.dxnnet.com
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EWMA 2013 - Ep563 - A new anti-biofilm dressing: in vivo investigationEWMAConference
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Thomas Mustoe2 MD, FACS
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Anti-Adhesion and Anti-Biofilm Effectiveness of Disinfectants Used In Hemodia...IJERA Editor
Biofilms are communities of microorganisms attached to a surface and included in an extracellular matrix making it resistant to exogenous deleterious agents. The aim of this study is to evaluate the anti-adhesive and anti-biofilm effect of five commercials disinfectants having different active principles (hydrogen peroxide, sodium hypochlorite, isopropyl alcohol and ethanol) on four Staphylococcus strains isolated from hemodialysis unit surfaces. The disinfectants anti-adhesive effect was estimated to an exceeding rate 70% for the various studied dilutions and 90% towards the pure products. Whereas the anti-biofilm effect showed an elimination rate varying between 10 % and 95 % according to the following parameters: active principle, time of contact, concentration and bacterial strain. Our study demonstrated that all tested products have an interesting anti-adhesive effect and that the peroxide of hydrogen is endowed with important anti-biofilm efficiency, followed by the alcoholic products and the sodium hypochlorite.
EWMA 2013-Ep443-DETERMINATION OF THE FLUID HOLDING CAPACITY (FHC) OF A NEW DE...EWMA
C. Wiegand1, K. Reddersen1, M. Abel2, Muldoon J3, P. Ruth2, U.-Ch. Hipler1
1Department of Dermatology, University Medical Center Jena, Jena, Germany
2Lohmann & Rauscher GmbH & Co. KG, Rengsdorf, Germany
3Activa Healthcare, Burton upon Trent, United Kingdom
EWMA 2013-Ep443-DETERMINATION OF THE FLUID HOLDING CAPACITY (FHC) OF A NEW DE...EWMAConference
DETERMINATION OF THE FLUID HOLDING CAPACITY (FHC) OF A NEW DEBRIDER* COMPARED TO CONVENTIONAL COTTON GAUZE
Cornelia Wiegand1, Kirsten Reddersen1, Martin Abel2, Jeanette Muldoon3, Peter Ruth2, Uta-Christina Hipler1
1Department of Dermatology, University Medical Center Jena (Jena, Germany);
2Lohmann & Rauscher GmbH & Co.KG (Rengsdorf, Germany);
3Activa Healthcare (Burton upon Trent, United Kingdom).
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2 Coloplast GmbH, Hamburg, Germany
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Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
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Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
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EWMA 2014 - EP402 IN VITRO EVALUATION OF THE CAPACITY OF A, MONOFILAMENT DEBRIDER* TO REMOVE BIOFILM AND THE EFFICACY OF DIFFERENT WOUND DRESSINGS TO PREVENT BIOFILM RE-GROWTH
1. Klinik für Hautkrankheiten
K. Reddersen1, C. Wiegand1, M. Abel2, J. Muldoon3, P. Ruth2, U.-Ch. Hipler1
1Department of Dermatology, University Medical Center Jena, Jena, Germany
2Lohmann & Rauscher GmbH & Co. KG, Rengsdorf, Germany
3Activa Healthcare, Burton upon Trent, United Kingdom
In vitro evaluation of the capacity of a
monofilament debrider* to remove biofilm and
the efficacy of different wound dressings to
prevent biofilm re-growth
2. Klinik für Hautkrankheiten
2
Introduction
Development of biofilms on wounds is a major impediment
to wound healing. Therefore, current research targets
antibiofilm strategies to restore an optimal wound-healing
environment. Combined treatment involving debridement
and addition of antibacterial agents may provide the
highest success rates. A monofilament debrider* consisting
of polyester fibres presents a fast and almost painless
option for debridement and removal of biofilm. We have
then analyzed the re-growth properties of biofilm
underneath different wound dressings.
Figure 1: Mechanical debridement (A) with the monofilament
debrider* (B). The debrider consists of polyester fibers (C).
2
A
B C
Material & Methods
A S. aureus biofilm was cultivated on glass plates (figure
2). The monofilament debrider* was used to wipe the glass
plates under standardized conditions (p=0.067N/cm2,
v=1.6cm/s). Afterwards, glass plates were covered with
various antimicrobially active wound dressings# and
incubated for 24h at 37°C. Then, dressings were removed
and glass plates further incubated for 48h. Biofilm on the
glass plates was evaluated directly after dressing removal
and following 48h re-growth period using the fluorescent
alamar blue assay.
*Debrisoft®, Lohmann & Rauscher
#A: Vliwasorb® (Lohmann & Rauscher), B: Vliwaktiv® (Lohmann & Rauscher), C: Vliwaktiv® Ag (Lohmann & Rauscher), D: Suprasorb® A (Lohmann & Rauscher), E: Suprasorb®
A + Ag (Lohmann & Rauscher), F: Suprasorb® X (Lohmann & Rauscher), G: Suprasorb® X + PHMB (Lohmann & Rauscher), H: Suprasorb® P (Lohmann & Rauscher)
3. Klinik für Hautkrankheiten
Results
It was shown that the monofilament debrider* effectively removed biofilm in vitro (figure 3). Furthermore, it was observed
that subsequent treatment with wound dressings reduced formation of new biomass (figure 4). Significantly fewer bacteria
were found after incubation with dressings containing antimicrobials like silver or polihexanide. The polihexanide-
containing dressing further exhibited a persistent decrease of biofilm re-growth, while biofilm quickly reformed in untreated
controls and after removal of antimicrobial-free and silver-containing dressings (figure 5).
Figure 2: Mature S. aureus biofilm on
glass plates after 48 hours of incubation
stained with SYTO-9/PI.
3
0
20
40
60
80
100
120
untreated monofilament debrider
biomass[%]
***A
B
Figure 3: Removal of biomass by
the monofilament debrider* (A). (B)
shows a representative example of
a glass plates after cleansing
(stained with SYTO-9/PI).
4. Klinik für Hautkrankheiten
Figure 4: Decrease of biomass on the debrided glass plates by
incubation with different wound dressings for 24 hours at 37°C.
4
Conclusion
It can be concluded that the combination of biofilm removal on the infected or critically colonized wound using a
monofilament debrider* and subsequent treatment with antimicrobial dressings presents a successful
antibiofilm strategy.
0
20
40
60
80
100
120
w/o
dressing
A B C D E F G H
biomass[%]
*
**
**
**
**
**
**
0
20
40
60
80
100
120
w/o
dressing
A B C D E F G H
biomass[%]
***
Figure 5: Regrowth of biomass is significantly inhibited after 48
hours by the PHMB-containing dressing G.