The document discusses an experiment to investigate how compressing cells by increasing the osmotic pressure outside the cells (adding PEG to remove water from cells) affects their actin bundle networks. Confocal microscopy images show that compressed cells on a gel substrate form more actin bundles and have higher actin fluorescence intensity than uncompressed cells. However, better methods are needed to precisely quantify changes in the actin networks. The compression may induce actin polymerization by changing the cell volume or ion concentration, rather than the substrate stiffness alone. Further study is still required to understand the mechanism of how actin polymerization is triggered.
Rheology is the study of the flow of matter, primarily in a liquid state, but also as "soft solids" or solids under conditions in which they respond with the plastic flow rather than deforming elastically in response to an applied force. Rheology is the science of deformation and flows within a material.
Graphic record heart sound - Phonogram.
Recording the sounds connected with the pumping action of heart.
Sound from heart – phonocardiogram
Instrument to measure this – phonocardiograph
Basic function – to pick up the different heart sound,filter the required and display.
MEASUREMENT OF BIO POTENTIAL USING TWO ELECTRODES AND RECORDING PROBLEMSBharathasreejaG
YOU CAN LEARN ABOUT MEASUREMENT USING TWO ELECTRODES & RECORDING PROBLEMS# NEED OF MEDICAL RECORDING # ELECTRODE TO SKIN INTERFACE # NERNST EQUATION # NOISE DURING RECORDING# MOTION ARTIFACT# ELECTRODE TO ELECTROLYTE NOISE # ELECTROLYTE TO SKIN NOISE# THERMAL NOISE# AMPLIFICATION NOISE# CABLE MOVEMENT# OTHER NOISES # CODING FOR GENERATING NOISE
Rheology is the study of the flow of matter, primarily in a liquid state, but also as "soft solids" or solids under conditions in which they respond with the plastic flow rather than deforming elastically in response to an applied force. Rheology is the science of deformation and flows within a material.
Graphic record heart sound - Phonogram.
Recording the sounds connected with the pumping action of heart.
Sound from heart – phonocardiogram
Instrument to measure this – phonocardiograph
Basic function – to pick up the different heart sound,filter the required and display.
MEASUREMENT OF BIO POTENTIAL USING TWO ELECTRODES AND RECORDING PROBLEMSBharathasreejaG
YOU CAN LEARN ABOUT MEASUREMENT USING TWO ELECTRODES & RECORDING PROBLEMS# NEED OF MEDICAL RECORDING # ELECTRODE TO SKIN INTERFACE # NERNST EQUATION # NOISE DURING RECORDING# MOTION ARTIFACT# ELECTRODE TO ELECTROLYTE NOISE # ELECTROLYTE TO SKIN NOISE# THERMAL NOISE# AMPLIFICATION NOISE# CABLE MOVEMENT# OTHER NOISES # CODING FOR GENERATING NOISE
This slide has been prepared in detaied manner and will help you.
The topics covered are:-
1- introduction
2.circuit diagram and its explaination
3.working
4. features
5.advantages / disadvantages
6. the top vendors
Basic MEP techniques and understanding for Intraoperative neuromonitoring of the motors tracts during Brain and Spinal surgeries to prevent postoperative complications.
Bio signal characteristics and recording modesBharathasreejaG
YOU CAN LEARN ABOUT BIO ELECTRIC SIGNAL CHARACTERISTICS # RECORDING MODES # BASICS OF BIOMEDICAL INSTRUMENTATION UNIT II CONTENTS # MEDICAL ELECTRONICS BIO ELECTRIC SIGNAL CHARACTERISTICS
This slide has been prepared in detaied manner and will help you.
The topics covered are:-
1- introduction
2.circuit diagram and its explaination
3.working
4. features
5.advantages / disadvantages
6. the top vendors
Basic MEP techniques and understanding for Intraoperative neuromonitoring of the motors tracts during Brain and Spinal surgeries to prevent postoperative complications.
Bio signal characteristics and recording modesBharathasreejaG
YOU CAN LEARN ABOUT BIO ELECTRIC SIGNAL CHARACTERISTICS # RECORDING MODES # BASICS OF BIOMEDICAL INSTRUMENTATION UNIT II CONTENTS # MEDICAL ELECTRONICS BIO ELECTRIC SIGNAL CHARACTERISTICS
6.Experiments with Xenopus (frog) in the late 1950s demonstrated tha.pdfwailesalekzydelore94
6.Experiments with Xenopus (frog) in the late 1950s demonstrated that cells within the 3 germ
layers had an affinity for each other and thus indicated that they had differentiated enough to be
“biased” toward a specific cell layer (endoderm, mesoderm, ectoderm). Describe an experiment
that demonstrated how the 3 different layers of the gastrula have an affinity for each other.
7.Morphogenesis involves the orchestration of Cell Affinity, Cell Adhesion, and Cell Migration.
How do the adhesive molecules play a role as cells of the embryo change their affinity for one
another?
PLEASE ANSWER BOTH QUESTIONS
Solution
1. Taking an advantage of discovery of amphibian tissues become dissociated into single cells
when placed in alkaline solupans,
2. The prepared single-cell suspensions from each of three germ layers of amphibian embryos
soon after the neural tube had formed.
3. Take two or more of these single-cell suspensions could be combined in various ways. When
the pH of the solution was normalized, the cells adhered to one another, forming aggregates on
agar-coated petri dishes.
4. This experiment shows affinity of 3 different layers of the gastrula.
Cell Affinity: a) The inner surface of the ectoderm has a positive affinity for mesodermal cells
and a negative affinity for the endoderm.
b) While the mesoderm has positive affinities for both ectodermal and endodermal cells.
c) Mimicry of normal embryonic structure by cell aggregates is also seen in the recombination of
epidermis and neural plate cells.
Cell Adhesion: a) cadherins are calcium-dependent adhesion molecules. They are critical for
establishing
and maintaining intercellular connections, and they appear to be crucial to the spatial
segregation of cell types and to the organization of animal form.
b) The cadherins are anchored inside the cell by a complex of proteins called catenins and the
cadherin-catenin complex forms the classic adherens junctions that help hold epithelial cells
together.
Cell Migration: a) migration involves the adhesion of the cell to its extracellular substrate. The
moving cell needs
something to push on, and attaches to the surrounding matrix.
b) Integrins span the cell membrane, connecting the extracellular matrix outside the cell to the
actin cytoskeleton on the inside of the cell. These connections of actin to integrin form focal
adhesions on the cell membrane where the membrane contacts the extracellular matrix.
c) Myosin and its regulators provide the motive force along these actin microfilaments.they are
linked with the lamellipodial actin at the sites of adhesion.
d) Release of adhesions in the rear, allowing the cell to migrate in the forward direction. It is
probable that stretch-sensitive calcium channels are opened and that the released calcium ions
activate proteases that destroy the focal adhesion sites..
Use of Surface Plasmon Resonance for Probing Cell-Matrix InteractionsReichertSPR
Reichert Technologies Life Sciences is sponsored a free educational webinar, “Use of Surface Plasmon Resonance for Probing Cell-Matrix Interactions,” which will demonstrated the use of SPR for the measurement of cell adhesion interactions in varied biomedical applications, and presented two examples in which the Reichert SPR system has been used for studying cells. The first example discussed human white blood cell (HL-60) adhesion/capture by the endothelial cell adhesion molecule P-selectin. Several antibody- and recombinant protein-based controls were used to demonstrate the application of SPR for human vascular-biology research. The second example discussed binding interactions between endothelial cells and two different extracellular matrix proteins, Collagen 1 and Matrigel.
It discuss about the cell, cell theory, Cell Theory Timeline,CELL DIVERSITY- unicellular, multicellular, size of cells, size of cells in human, shape of cell, structure of cell, Microscope, CELL CONSTITUENTS- cell wall/cell membrane, nucleus, cytoplasm in detail
Smart TV Buyer Insights Survey 2024 by 91mobiles.pdf91mobiles
91mobiles recently conducted a Smart TV Buyer Insights Survey in which we asked over 3,000 respondents about the TV they own, aspects they look at on a new TV, and their TV buying preferences.
UiPath Test Automation using UiPath Test Suite series, part 3DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 3. In this session, we will cover desktop automation along with UI automation.
Topics covered:
UI automation Introduction,
UI automation Sample
Desktop automation flow
Pradeep Chinnala, Senior Consultant Automation Developer @WonderBotz and UiPath MVP
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
UiPath Test Automation using UiPath Test Suite series, part 4DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 4. In this session, we will cover Test Manager overview along with SAP heatmap.
The UiPath Test Manager overview with SAP heatmap webinar offers a concise yet comprehensive exploration of the role of a Test Manager within SAP environments, coupled with the utilization of heatmaps for effective testing strategies.
Participants will gain insights into the responsibilities, challenges, and best practices associated with test management in SAP projects. Additionally, the webinar delves into the significance of heatmaps as a visual aid for identifying testing priorities, areas of risk, and resource allocation within SAP landscapes. Through this session, attendees can expect to enhance their understanding of test management principles while learning practical approaches to optimize testing processes in SAP environments using heatmap visualization techniques
What will you get from this session?
1. Insights into SAP testing best practices
2. Heatmap utilization for testing
3. Optimization of testing processes
4. Demo
Topics covered:
Execution from the test manager
Orchestrator execution result
Defect reporting
SAP heatmap example with demo
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
Generating a custom Ruby SDK for your web service or Rails API using Smithyg2nightmarescribd
Have you ever wanted a Ruby client API to communicate with your web service? Smithy is a protocol-agnostic language for defining services and SDKs. Smithy Ruby is an implementation of Smithy that generates a Ruby SDK using a Smithy model. In this talk, we will explore Smithy and Smithy Ruby to learn how to generate custom feature-rich SDKs that can communicate with any web service, such as a Rails JSON API.
Neuro-symbolic is not enough, we need neuro-*semantic*Frank van Harmelen
Neuro-symbolic (NeSy) AI is on the rise. However, simply machine learning on just any symbolic structure is not sufficient to really harvest the gains of NeSy. These will only be gained when the symbolic structures have an actual semantics. I give an operational definition of semantics as “predictable inference”.
All of this illustrated with link prediction over knowledge graphs, but the argument is general.
Transcript: Selling digital books in 2024: Insights from industry leaders - T...BookNet Canada
The publishing industry has been selling digital audiobooks and ebooks for over a decade and has found its groove. What’s changed? What has stayed the same? Where do we go from here? Join a group of leading sales peers from across the industry for a conversation about the lessons learned since the popularization of digital books, best practices, digital book supply chain management, and more.
Link to video recording: https://bnctechforum.ca/sessions/selling-digital-books-in-2024-insights-from-industry-leaders/
Presented by BookNet Canada on May 28, 2024, with support from the Department of Canadian Heritage.
Kubernetes & AI - Beauty and the Beast !?! @KCD Istanbul 2024Tobias Schneck
As AI technology is pushing into IT I was wondering myself, as an “infrastructure container kubernetes guy”, how get this fancy AI technology get managed from an infrastructure operational view? Is it possible to apply our lovely cloud native principals as well? What benefit’s both technologies could bring to each other?
Let me take this questions and provide you a short journey through existing deployment models and use cases for AI software. On practical examples, we discuss what cloud/on-premise strategy we may need for applying it to our own infrastructure to get it to work from an enterprise perspective. I want to give an overview about infrastructure requirements and technologies, what could be beneficial or limiting your AI use cases in an enterprise environment. An interactive Demo will give you some insides, what approaches I got already working for real.
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
Securing your Kubernetes cluster_ a step-by-step guide to success !KatiaHIMEUR1
Today, after several years of existence, an extremely active community and an ultra-dynamic ecosystem, Kubernetes has established itself as the de facto standard in container orchestration. Thanks to a wide range of managed services, it has never been so easy to set up a ready-to-use Kubernetes cluster.
However, this ease of use means that the subject of security in Kubernetes is often left for later, or even neglected. This exposes companies to significant risks.
In this talk, I'll show you step-by-step how to secure your Kubernetes cluster for greater peace of mind and reliability.
Securing your Kubernetes cluster_ a step-by-step guide to success !
Cell Mechanics
1. Compression induced formation of
Actin bundle networks in Adherent
Cells
Burhan Saif Addin
Ming Guo, Weitz Lab
Harvard University
ES 298r
Mar 23, 2012
2. Outline
• Introduction and Motivation
• Background Information
• Project Idea
• Results and Discussion
• Conclusion
1
3. Introduction
• What is the Cytoskeleton (CSK) :
– 3D protein scaffold. en.wikipedia.org/wiki/Cytoskeleton
– Determine structure, shape, organization, ....
– More than 8% of Human Genome encodes for cytoskeleton
– Cytoskeleton mechanics are not well understood and the search for
universal laws , if any, is in progress.
– Contain three major skeletal proteins: actin, intermediate filaments, and
microtubules.
• What is role of Actin Filaments :
– Cell motility and adhesion
– Cell mechanical response and modulation.
2
4. Motivation: Why study cells actin networks?
• How life evolved ?
• Morphogenesis ?
• How cytoskeleton work ? Cell Motility, etc ?
• How Cells sense and process information ?
• To understand Cell Mechanics and stiffness:
– Physics not well understood. Are there universal laws ?
– Understand Diseases. For example: Malaria and Cancer.
– Cells signaling and gene expressions.
3
5. Adherent Cells feel and respond to the
stiffness of their substrate
Esoft~ 1kPa Estiff~ 30-100kPa
Stress versus strain illustrated for different soft tissues
Mechanism for Cellular response to substrate stiffness
are not well understood. Possible Mechanisms:
-Molecular pathways.
-Volume change ?
4
“Tissue Cells Feel and Respond to the Stiffness of Their Substrate” Discher et al, Science, 18 Nov 2005.
6. How do we know that reducing the volume of
the cell induce actin formation ?
• In actin solution droplets, Huber et al showed that increased ion
concentration and actin concentration led to highly ordered and regularly
spaced actin bundle networks without the need for crosslinkers or motors.
• In this work, we compress the cell by increasing the osmotic pressure of
the cell’s medium by adding PEG (suck water out of cell) to the solution.
Kas et al. Biophysics Society Meeting 2012, San Diego.
“Counterion Induced formation of regular actin bundle networks”. Hubber et al. Soft Materials. March 2012 5
7. Cells on Glass without compression
6
Images are by confocal fluorescence microscopy
9. Compressed Cells on Gel (10% PEG solution)
Volume decrease might be the mechanism that induce actin networks
formation thus cells stiffness.
8
10. Green line Actin’s Dye Fluorescence Intensity across the lines in
is ~31 μm) the left figure which are cell’s cross-sections.
.
Rough Results:
Without compression:
0.28 peaks/um
With compression:
1.72 peaks/um
9
11. Possible Problems with this method of dye
intensity quantization
• Reproducibility.
• Highly dense Interlinked actin filaments.
• Unlabeled actin filaments might not be
uniform*.
*Polar patterns of driven filaments. Nature. Volker
Schaller et al. 24 June 2010. 10
12. Other method for quantifying actin
bundles networks ?
We attempted to quantify
the Actin based on intensity.
How do you quantify this ?
“Counterion Induced formation of regular actin bundle 11
networks”. Hubber et al. Soft Materials. March 2012
13. Conclusion
• Volume change in cell can change Actin
concentration or ion concentration, and hence
induce actin polymerization.
• Perhaps the substrate stiffness effect is not
the only reason for actin polymerization.
• Is actin polymerization due to volume change
or due substrate or chemical signaling ? This
remain to be investigated.
12
What is actin ? actin , a biopolymer, is found in the cytoskeleton. The sperical G-actin moleculuespolymerise to from F-Actin filaments which bundle together.CSKmechnics is dominated by actin bundle netwroks. It has a complictaed stress-strain respoonse, The cell soften if the strain was transient becoming fluid like.
One of the oldest polymer , exist on Eukaryotic cells,Mechanical Modulation Physical properties of tissues and cells change with disease For exampleCancer whichkills more than 7 million per and one way study Cancer metastisis as function of cells stiffness. Too low stiffnes can akecancrous cells squeese through the body easily Malaria which killls more than 1 million a yearMalrai infected cells are stiffer than health ones which make then un able to squeeze through blood capiliaries
Human walk differently on different substrates : sand, paved, gel. The same for cells, particularly adherent cells. Substrate stiffness influnce adhesion strucutre, cell dynamics and sytoskeletonassmply and orgniation and cell spreading. Dynamic adhesion on soft substrate , static focal adhesion on stiffsubstrate. Fig.2 Substrate stiffness influences adhesion structures and dy- namics (14), cytoskeleton assembly and cell spreading (17, 42), and differentiation processes such as striation of myotubes (28). (Top) The arrows point to dynamic adhesions on soft gels and static, focal adhesions on stiff gels. [Adapted from (14)] (Middle) The actin cyto- skeleton. (Bottom) A cell-on-cell layering in which the lower layer is attached first to glass so that the upper layer, which fuses from myoblasts that are added later, perceives a soft, cellular substrate.Cell recognize the underlying substrate composition Cells need to adhere to a solid to function. “Anchorage dependance “ they are not viable when suspended in fluid The mechanical response of cells is dependent on the substrate and the whole extracceullular matrix
In actin solution droplets, Huber et al showed that increased ion concentration led to highly ordered and regularly spaced actin bundle networks without the need for molecular motors. The same phenomena should happen to any volume decreased cell, on any substrate. We compress the cell by increasing the osmotic pressure of the cell’s medium by adding PEG to the solution. Fig. 1 Experimental procedure. Using a glass micropipette chilled G-actin solution is transferred onto a passivated glass substrate which is covered by a layer of hexadecane or silicone oil (A). Actin is allowed to polymerize and reach steady state conditions at room temperature (B). By removing some of the oil, evaporation from the droplet through the remaining oil layer is accelerated (C). When reaching the bundling threshold of the multivalent ion concentration an extended network of actin bundles appears throughout the whole droplet within minutes (E). The droplet volume can be monitored over time based on confocal images (D, squares) giving access to actual actin and ion concentrations (D, circles). In addition to a direct visualization (E), the intensity deviation of the fluorescence signal (D, triangles) presents a measure for the transition from F-actin to bundle networks
Images clearly shos less dense actin filaments on gel relative to glassWhen we compress the cells, by putting 10% peg into the soltuion leaving for about 10 minutes , we also note that
It is known that sustained pressure, increases cells stiffness. This may induce actin bundles network formation. The mehanisim for stiffness migght be , also the volume decrease increase the concentration Universal physical responses to stretch in the living cell . Trepat et al. Nature. Vol 447, 31 May 2007.
Ask for better ideas on how to quantify actin bundles
Ask for better ideas on how to quantify actin bundles