Enzyme immobilization involves restricting the movement of enzymes by confining them to a solid support. This allows for repeated use of enzymes, enhanced stability, and easy separation of products. Common immobilization methods include physical adsorption, covalent bonding, cross-linking, and entrapment. Immobilized enzymes have various applications, such as isomerization of glucose to fructose using immobilized glucose isomerase, and use of immobilized urease in compact artificial kidneys. Immobilization provides benefits like recyclability and cost-efficiency compared to free enzymes.
Enzyme definition, Enzyme immobilization introduction , Enzyme immobilization definition, Explanation about support/ matrix, Examples about immobilized enzymes and their product, Advantages of immobilization, Applications of immobilization, Methods of immobilization in different categories like Adsorption method, Covalent bonding method, Entrapment method, Co polymerization /Cross linking method, Encapsulation method, Applications of immobilized enzymes, Diagrammatic explanation about methods of immobilization.
As enzymes are biological catalysts that promote the rate of reactions but are not themselves consumed in the reactions; they may be used repeatedly for as long as they remain active. However, in most of the processes, enzymes are mixed in a solution with substrates and cannot be economically recovered after the reaction and are generally wasted. Thus, there is an incentive to use enzymes in an immobilized or insolubilized form so that they may be retained in a biochemical reactor for further catalysis.
Enzyme immobilization may be defined as a process of confining the enzyme molecules to a solid support over which a substrate is passed and converted to products. The process whereby the movement of enzymes, cells, organelles, etc. in space is completely or severely restricted usually resulting in a water-insoluble form of the enzyme
Protein engineering is the process of developing useful or valuable proteins. It is a young discipline, with much research taking place into the understanding of protein folding and recognition for protein design principles
Microbes, or microscopic organisms, are widely used in large-scale industrial processes. Microbes can be used to create biofertilizers or to reduce metal pollutants. Microbes can also be used to produce certain non-microbial products, such as the diabetes medication insulin, vaccines, etc. These slides will give insights into uses of microbes in production of enzymes, antibiotics, beverages, vitamins, vaccines, probiotics, etc
UNIT 6 Fermentation technology, Fermenters, Study of Media, types of fermenta...Shyam Bass
UNIT-6 6th Sem B.Pharma Pharmaceutical Biotechnology-
Following slides include-
Fermentation technology and biotechnological products :
Fermentation methods and general requirements
Study of media
Equipment
Sterilization methods
Aeration process
Stirring
large scale production fermenter design and its various controls
BY- SHYAM BASS
Immobilization techniques are used by the movement of cells and enzymes. First enzyme immobilized in Japan. The immobilized enzyme is aminoacylase. This process is carried out by various methods such adsorption, covalent bonding, cross linking, entrapment, encapsulation. Immobilization of cells is an alternative method for immobilization of enzymes.
Enzyme definition, Enzyme immobilization introduction , Enzyme immobilization definition, Explanation about support/ matrix, Examples about immobilized enzymes and their product, Advantages of immobilization, Applications of immobilization, Methods of immobilization in different categories like Adsorption method, Covalent bonding method, Entrapment method, Co polymerization /Cross linking method, Encapsulation method, Applications of immobilized enzymes, Diagrammatic explanation about methods of immobilization.
As enzymes are biological catalysts that promote the rate of reactions but are not themselves consumed in the reactions; they may be used repeatedly for as long as they remain active. However, in most of the processes, enzymes are mixed in a solution with substrates and cannot be economically recovered after the reaction and are generally wasted. Thus, there is an incentive to use enzymes in an immobilized or insolubilized form so that they may be retained in a biochemical reactor for further catalysis.
Enzyme immobilization may be defined as a process of confining the enzyme molecules to a solid support over which a substrate is passed and converted to products. The process whereby the movement of enzymes, cells, organelles, etc. in space is completely or severely restricted usually resulting in a water-insoluble form of the enzyme
Protein engineering is the process of developing useful or valuable proteins. It is a young discipline, with much research taking place into the understanding of protein folding and recognition for protein design principles
Microbes, or microscopic organisms, are widely used in large-scale industrial processes. Microbes can be used to create biofertilizers or to reduce metal pollutants. Microbes can also be used to produce certain non-microbial products, such as the diabetes medication insulin, vaccines, etc. These slides will give insights into uses of microbes in production of enzymes, antibiotics, beverages, vitamins, vaccines, probiotics, etc
UNIT 6 Fermentation technology, Fermenters, Study of Media, types of fermenta...Shyam Bass
UNIT-6 6th Sem B.Pharma Pharmaceutical Biotechnology-
Following slides include-
Fermentation technology and biotechnological products :
Fermentation methods and general requirements
Study of media
Equipment
Sterilization methods
Aeration process
Stirring
large scale production fermenter design and its various controls
BY- SHYAM BASS
Immobilization techniques are used by the movement of cells and enzymes. First enzyme immobilized in Japan. The immobilized enzyme is aminoacylase. This process is carried out by various methods such adsorption, covalent bonding, cross linking, entrapment, encapsulation. Immobilization of cells is an alternative method for immobilization of enzymes.
enzyme immobilization, pharmaceutical biotechnology, b pharam 6th sem, PCI.
history,
why immobilise
advantages and disadvantages of enzyme immobilization
methods of enzyme immobilization
physical retention
entrapment
microencapsulation
chemical bonding
adsorption
cross linking
covalent binding
ionic binding
Applications of Immobilized Enzymes
Enzyme utilization in Industry
ENZYMES are large biological molecules (catalysts) that speed up rate of chemical reaction without being used up in the reaction. (responsible for the thousands of chemical interconversions that sustain life).
IMMOBILIZATION means that the biocatalysts are limited in moving due to chemically or physically treatment.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
1. ENZYME BIOTECHNOLOGY -
Methods of Enzyme Immobilization & Its
Applications
Dr. Sowjanya Pulipati
Professor & Head
Dept. of Pharm Biotechnology
Vignan Pharmacy College
Vadlamudi, Guntur (Dt), A.P., INDIA.
2. An immobilized enzyme is one whose
movement in space has been restricted either
completely or to a small limited region.
Enzyme immobilization may be defined as a process of confining
the enzyme molecules to a solid support over which a substrate is
passed and converted to products. The process whereby the
movement of enzymes, cells, organelles, etc. in space is completely
or severely restricted usually resulting in a water-insoluble form of
the enzyme
What Is An Immobilized Enzyme?
What Is Enzyme Immobilization ?
3. Need for Immobilization
Protection from degradation and deactivation.
Recycling, repetitive use.
Cost efficiency.
Enhanced stability.
Use as controlled release agents.
The ability to stop the reaction rapidly by removing
the enzyme from the reaction Solution (or vice-versa)
Allows development of multi-enzyme
reaction system.
4. SALIENT FEATURES OF ENZYME IMMOBILIZATION
The enzyme phase is called as carrier phase which is water insoluble
but hydrophilic porous polymeric matrix, e.g. agarose, cellulose, etc.
The enzyme phase may be in the form of fine particulate, membranous
or microcapsule.
The enzyme in turn may be bound to another enzyme via cross linking.
A special module is produced employing immobilization techniques
through which fluid can pass easily, transforming substrate into
product and at the same time facilitating the easy removal of catalyst
from the product as it leaves the reactor.
5. The support or carrier utilized in immobilization technique is not stable at
particular PH, ionic strength or solvent conditions. Hence, may be disrupted or
dissolved releasing the enzyme component after the reaction.
Advantages of enzyme immobilization:-
Multiple or repetitive use of a single batch of enzymes.
Immobilized enzymes are usually more stable.
Ability to stop the reaction rapidly by removing the enzyme from the reaction
solution.
Product is not contaminated with the enzyme.
Easy separation of the enzyme from the product.
Allows development of a multi enzyme reaction system.
Reduces effluent disposal problems.
6. Disadvantages of enzyme immobilization:-
It gives rise to an additional bearing on cost.
It invariably affects the stability and activity of enzymes.
The technique may not prove to be of any advantage when one
of the substrate is found to be insoluble.
Certain immobilization protocols offer serious problems with
respect to the diffusion of the substrate to have an access to the
enzyme.
7.
8. PHYSICAL ADSORPTION
This method is based on the physical adsorption
of enzyme protein on the surface of water-
insoluble carriers.
Examples of suitable adsorbents are ion-
exchange matrices, porous carbon, clay, hydrous
metal oxides, glasses and polymeric aromatic
resins.
The bond between the enzyme and carrier
molecule may be ionic, covalent, hydrogen,
coordinated covalent or even combination of
any of these.
9. Method of immobilization by adsorption
• This method is based on the physical adsorption of enzyme
protein on the surface of water-insoluble carriers.
1
• The enzyme is adequately mixed with a right adsorbent
under appropriate PH & ionic strength.
2
• After incubation for certain period of time, the carrier
matrix is washed thoroughly to get rid of entire
unadsorbed enzyme molecules whereby the immobilized
enzyme is ready for actual usage.
3
• This specific method gives rise to high loading (1gm
enzyme/gm matrix) of enzyme.
4
10. Advantages of adsorption:-
Little or no confirmation change of the enzyme.
Simple and cheap.
No reagents are required.
Wide applicability and capable of high enzyme
loading.
Disadvantages of adsorption:-
Desorption of the enzyme protein resulting from
changes in temperature, PH and ionic strength.
Slow method.
12. Enzymes immobilized by adsorption
S.No Name of the enzyme Adsorbent
1 α-Amylase Calcium phosphate
2 Amyloglucosidase
Agarose, DEAE-sephadex
(Diethylaminoethyl)
3 Catalase Charcoal
4 Glucose oxidase Cellophane
5 Invertase
Charcoal, DEAE-
sephadex
13. Covalent bonding
Widely used
The covalent bond between enzyme and a
support matrix forms a stable complex.
The functional group present on enzyme,
through which a covalent bond with support
could be established, should be non essential
for enzymatic activity.
The most common technique is to activate a
cellulose-based support with cyanogen
bromide, which is then mixed with the
enzyme.
14. The protein functional groups which could be
utilized in covalent coupling include:
Amino group
Carboxylic group
Phenol ring
Indole group
Imidazole group
The most commonly used polymers are
polysaccharides, polyvinyl alcohol, silica
and porous glasses.
19. Advantages of covalent bonding:-
The strength of binding is very strong,
so, leakage of enzyme from the support is
absent or very little.
This is a simple, mild and often successful
method of wide applicability
Disadvantages of covalent coupling:-
Enzymes are chemically modified and so many
are denatured during immobilization.
Only small amounts of enzymes may be
immobilized (about 0.02 grams per gram of
matrix).
20. Cross linking
This method is based on the formation of covalent
bonds between the enzyme molecules, by means of
multifunctional reagents, leading to three
dimensional cross linked aggregates.
The most common reagent used for cross-linking is
glutaraldehyde.
21. Cross linking
Advantages of cross linking:-
Very little desorption(enzyme strongly bound)
Best used in conjunction with other methods.
Disadvantages of cross linking:-
Cross linking may cause significant changes in
the active site.
22. Entrapment
The enzymes is trapped inside
the polymer matrix.
Biocatalyst + monomer solution
Polymerization
(Change in temp, PH)
Enzyme entrapment
Polymers – polyacrylamide,
collagen, cellulose acetate,
calcium alginate or carrageenan
etc are used as the matrices.
23. ENTRAPMENT
• Entrapment in gel may cause:
– Matrix polymerization or
– Precipitation or
– Coagulation
• Entrapment in calcium alginate is most widely
used entrament for:
– Microbial
– Animals
– Plant enzymes/cells
Ex: Glucose oxidase + Polyacrlamide
(entapment gel)
24. Advantages of entrapment:-
Loss of enzyme activity upon immobilization is
minimized.
Disadvantages of entrapment:-
The enzyme can leak into the surrounding
medium.
Another problem is the mass transfer resistance
to substrates and products.
Substrate cannot diffuse deep into the gel matrix.
25.
26. 1. Occlusion within a cross linked gel:-
In this entrapment method, a highly cross-linked
gel is formed as a result of the polymerization
which has a fine "wire mesh" structure and can
more effectively hold smaller enzymes in its
cages.
Amounts in excess of 1 g of enzyme per gram of
gel or fibre may be entrapped.
Some synthetic polymers such as polyacrylamide,
polyvinylalcohol, etc... and natural polymer
(starch) have been used to immobilize enzymes
using this technique.
27. 2. Microencapsulation:-
This entrapment involves the formation of
spherical particle called as “microcapsule” in
which a liquid or suspension of biocatalyst is
enclosed within a semi permeable polymeric
membrane.
28.
29. Applications of Enzyme Immobilization
Enzyme Method of
Immobilization
Applications
Glucose
isomerase
Embedment in
collagen matrix
concentration
Isomerization of glucose to
fructose in production of high
fructose syrup which is
sweeter
Aminoacylase Embedment in
collagen matrix
concentration
Resolution of racemic mixture
of aminoacids to give L-
aminoacids which have
nutritional value
Glucose
oxidase
Entrapment in
polyacrylamide gel
and layered over
O2 electrode
Determination of glucose
concentration
30. Applications of Enzyme Immobilization
Enzyme Method of
Immobilization
Applications
Urease Layered on
Platinum/O2 electrode
Determination of urea
and uric acid
concentration
Alcohol
oxidase
Layered on
Platinum/O2 electrode
Assay of alcohols
Alcohol
phosphatase
Layered on
Platinum/O2 electrode
Assay of glucose-6-
phosphate
Penicillinase Layered on PH
electrode
Assay of penicillin
31. Applications of Enzyme Immobilization
Enzyme Method of
Immobilization
Applications
Urokinase/
Streptokinase
Adsorbed on Sephadex Breakdown of
thromboemboli
α-Amylase,
catalase, trypsin
Adsorbed on dextran Have slower clearance
from blood
Phenylalanine
ammonia
Encapsulation in
microencapsules, fibres
or gels
Reduction of blood
levels of phenylalanine
Urease +
adsorbed resin
or charcoal
microencapsulation Compact artificial
kidney