ELISA (enzyme-linked immunosorbent assay) is an analytical biochemistry technique used to detect the presence of antibodies or antigens in a sample through the use of enzymes. It involves immobilizing an antigen or antibody on a plate and detecting it using an antibody or antigen that is linked to an enzyme. This allows the detection of the binding reaction through color change or fluorescence when a substrate is added. ELISA is a highly sensitive and specific technique used to detect various targets including hormones, proteins, antibodies, and pathogens in samples like serum, plasma, cell lysates, and more. It has applications in areas like endocrinology, infectious disease testing, food safety, and plant pathology.
Mr. Shubham Khairnar presented on ELISA (Enzyme-Linked Immunosorbent Assay), an immunoassay technique used to detect antibodies, proteins, and biomolecules. ELISA uses antibodies and color changing enzymes to detect the presence of a substance. It has advantages like being highly specific and sensitive, having a long shelf life, and being easy to perform. Common steps in ELISA include coating wells with antigens or antibodies, washing, incubating with samples and secondary antibodies, developing color, and reading results. ELISA has many applications like detecting viral infections, hormone levels, allergens, and more.
ELISA, or enzyme-linked immunosorbent assay, is an immunoassay technique used to detect the presence of antibodies or antigens in a sample. In ELISA, an antigen or antibody is attached to a plastic plate and then exposed to a sample. If the target antigen or antibody is present, it will bind. A labeled secondary antibody is then added to detect the bound antigen-antibody complex. The presence of the complex is then indicated by a color change reaction from an enzyme attached to the secondary antibody reacting with a substrate. ELISA is a highly sensitive and specific technique used to detect proteins, peptides, biomolecules, antibodies, and more in applications like disease diagnosis, drug testing, food safety testing, and research
The document provides an introduction to ELISA (enzyme-linked immunosorbent assay), which is a biochemical technique used mainly in immunology to detect the presence of an antibody or antigen in a sample. It describes the basic principles and steps of the ELISA process, which involves detecting antibodies or antigens using an enzyme-labeled secondary antibody and color changing reaction. Key aspects covered include antigen-antibody binding, use of enzyme labels, substrate conversion, and quantitative/qualitative applications of ELISA for detecting various molecules.
These slides made by Miss Khunsha Fatima. This presentation will cover mainly ELISA, its Basic Principle, Steps and its related topics discussed in detail.
ELISA, or enzyme-linked immunosorbent assay, is a quantitative immunological technique used to detect antibodies or antigens in a sample. It involves immobilizing an antigen or antibody on a plate and detecting it using an enzyme-linked antibody or antigen. The enzyme's activity is then measured using a chromogenic substrate, producing a detectable color change if the antigen-antibody complex is present. ELISA has applications in detecting hormones, proteins, infectious agents, drugs, and more due to its high sensitivity and specificity. It is a widely used, inexpensive, and easy to perform technique.
1. ELISA (Enzyme-linked Immunosorbent Assay) is an immunoassay technique used to detect the presence of antibodies and antigens in a solution. It relies on antibodies and antigens binding specifically together.
2. There are several types of ELISA including direct, indirect, sandwich, competitive, and reverse ELISA. Each uses a different approach but all rely on an enzyme-labeled antibody or antigen binding to produce a detectable color change.
3. ELISA is a very sensitive technique that can detect ng/mL to pg/mL concentrations and is widely used for applications like detecting hormones, infectious agents, drugs and more.
The document provides a history of ELISA (Enzyme-Linked ImmunoSorbent Assay) and describes its components, procedures, types, applications and advantages. It discusses key events in immunology research from the 18th century leading to the development of ELISA in the 1970s. ELISA allows detection and quantification of antigens or antibodies in a simple, sensitive and cost-effective manner using enzyme-labeled antibodies or antigens.
ELISA (Enzyme-Linked Immunosorbent Assay) is a biochemical technique used to detect antibodies or antigens in a sample. There are three main types of ELISA: competitive ELISA, sandwich ELISA, and indirect ELISA. Sandwich ELISA coats a plate with capture antibodies and detects antigens bound between the capture and detection antibodies. Indirect ELISA coats antigens on a plate and detects antibodies in samples using enzyme-linked secondary antibodies. ELISA is used to test samples like blood, urine, and tissue extracts for proteins, hormones, antibodies, and other molecules.
Mr. Shubham Khairnar presented on ELISA (Enzyme-Linked Immunosorbent Assay), an immunoassay technique used to detect antibodies, proteins, and biomolecules. ELISA uses antibodies and color changing enzymes to detect the presence of a substance. It has advantages like being highly specific and sensitive, having a long shelf life, and being easy to perform. Common steps in ELISA include coating wells with antigens or antibodies, washing, incubating with samples and secondary antibodies, developing color, and reading results. ELISA has many applications like detecting viral infections, hormone levels, allergens, and more.
ELISA, or enzyme-linked immunosorbent assay, is an immunoassay technique used to detect the presence of antibodies or antigens in a sample. In ELISA, an antigen or antibody is attached to a plastic plate and then exposed to a sample. If the target antigen or antibody is present, it will bind. A labeled secondary antibody is then added to detect the bound antigen-antibody complex. The presence of the complex is then indicated by a color change reaction from an enzyme attached to the secondary antibody reacting with a substrate. ELISA is a highly sensitive and specific technique used to detect proteins, peptides, biomolecules, antibodies, and more in applications like disease diagnosis, drug testing, food safety testing, and research
The document provides an introduction to ELISA (enzyme-linked immunosorbent assay), which is a biochemical technique used mainly in immunology to detect the presence of an antibody or antigen in a sample. It describes the basic principles and steps of the ELISA process, which involves detecting antibodies or antigens using an enzyme-labeled secondary antibody and color changing reaction. Key aspects covered include antigen-antibody binding, use of enzyme labels, substrate conversion, and quantitative/qualitative applications of ELISA for detecting various molecules.
These slides made by Miss Khunsha Fatima. This presentation will cover mainly ELISA, its Basic Principle, Steps and its related topics discussed in detail.
ELISA, or enzyme-linked immunosorbent assay, is a quantitative immunological technique used to detect antibodies or antigens in a sample. It involves immobilizing an antigen or antibody on a plate and detecting it using an enzyme-linked antibody or antigen. The enzyme's activity is then measured using a chromogenic substrate, producing a detectable color change if the antigen-antibody complex is present. ELISA has applications in detecting hormones, proteins, infectious agents, drugs, and more due to its high sensitivity and specificity. It is a widely used, inexpensive, and easy to perform technique.
1. ELISA (Enzyme-linked Immunosorbent Assay) is an immunoassay technique used to detect the presence of antibodies and antigens in a solution. It relies on antibodies and antigens binding specifically together.
2. There are several types of ELISA including direct, indirect, sandwich, competitive, and reverse ELISA. Each uses a different approach but all rely on an enzyme-labeled antibody or antigen binding to produce a detectable color change.
3. ELISA is a very sensitive technique that can detect ng/mL to pg/mL concentrations and is widely used for applications like detecting hormones, infectious agents, drugs and more.
The document provides a history of ELISA (Enzyme-Linked ImmunoSorbent Assay) and describes its components, procedures, types, applications and advantages. It discusses key events in immunology research from the 18th century leading to the development of ELISA in the 1970s. ELISA allows detection and quantification of antigens or antibodies in a simple, sensitive and cost-effective manner using enzyme-labeled antibodies or antigens.
ELISA (Enzyme-Linked Immunosorbent Assay) is a biochemical technique used to detect antibodies or antigens in a sample. There are three main types of ELISA: competitive ELISA, sandwich ELISA, and indirect ELISA. Sandwich ELISA coats a plate with capture antibodies and detects antigens bound between the capture and detection antibodies. Indirect ELISA coats antigens on a plate and detects antibodies in samples using enzyme-linked secondary antibodies. ELISA is used to test samples like blood, urine, and tissue extracts for proteins, hormones, antibodies, and other molecules.
This presentation explains about the principle and procedure involved in elisa method of immunoassay, development o f elisa , application advantages and disadvantages of elisa
ELISA, or enzyme-linked immunosorbent assay, is an immunoassay technique used to detect the presence of antibodies or antigens in a biological sample. It involves an enzymatic reaction that produces a detectable color change if the target antibody or antigen is present. There are several types of ELISA including direct, indirect, sandwich, and competitive ELISA. ELISA tests are commonly used to screen blood donations, detect infections, measure hormone and toxin levels, and detect allergens and drugs.
ELISA involves an antigen-antibody reaction between a solid surface like a microwell plate coated with the target antigen and an enzyme-labeled antibody specific to that antigen. There are four main types of ELISA - direct, indirect, sandwich, and competitive - which differ in the order and number of antibodies used. ELISA is commonly used to detect antigens and antibodies and has applications in fields like medical diagnostics, food testing, and environmental monitoring.
Radioimmunoassay (RIA) is a sensitive technique introduced in 1960 that uses radioactive isotopes and antibodies to measure antigens or hormones. It involves competitive binding between radiolabeled and unlabeled antigens for antibodies. Enzyme-linked immunosorbent assay (ELISA) is a common technique introduced in 1971 that uses enzymes to detect and quantify substances like proteins. It can be indirect, sandwich, or competitive based on antigen-antibody binding structure. Both RIA and ELISA are sensitive techniques used to detect substances like hormones, vitamins, drugs, and antigens in clinical medicine and research.
ELISA (Enzyme-Linked Immunosorbent Assay) is a biochemical technique used to detect antibodies or antigens in a sample. There are three main types of ELISA: competitive ELISA where a labeled antigen competes for antibody binding sites; sandwich ELISA where antibodies coat a surface to detect a specific antigen; and indirect ELISA where a test antigen is coated on a surface and secondary antibodies are used to detect primary antibodies in a serum sample. The amount of color change produced corresponds to the concentration of antigen or antibody in the original sample.
The document provides an overview of enzyme-linked immunosorbent assay (ELISA), including its history, principles, requirements, types (direct, indirect, sandwich, competitive), applications (ELISPOT), equipment (washer, reader), and generations used for HIV detection. ELISA is a sensitive technique for detecting antigen-antibody interactions using an enzyme-substrate system for detection. It was developed in 1971 and involves multiple steps with different reagents to separate bound and unbound fractions.
elisaLecture_based on new way to medical lab.pptmainakg09
The document describes the enzyme-linked immunosorbent assay (ELISA) technique. ELISA uses antibodies and antigen-enzyme conjugates to detect the presence and quantify antigens or antibodies in a sample. It is a sensitive, specific immunoassay that is usually performed in microwell plates, where antibodies or antigens bind to the wells and are detected using enzyme-linked antibodies and color-changing substrates. There are different types of ELISA including sandwich, competitive, and indirect formats.
Antigen ,Antibody and Ag-Ab reactions ppt by DR.C.P.PRINCEDR.PRINCE C P
An immunogen refers to a molecule that is capable of eliciting an immune response, whereas an antigen refers to a molecule that is capable of binding to the product of that immune response (Ab).
So, an immunogen is necessarily an antigen, but an antigen may not necessarily be an immunogen
The terms immunogen and antigen are often used interchangeably but the later is more common.
Antibodies are Globulin Protein (Immunoglobulin) that are synthesized in the Serum and Tissue fluids.
It reacts specifically with the antigen that stimulated their production.
There are two types serum proteins: albumin and globulin
There are Three types of globulins .
1. Alpha globulin
2. Beta globulin
3. Gamma globulin (Antibodies)
Gamma globulins are responsible for immunity. So they are called as Immunoglobulin (Ig)
The binding of an antibody with an antigen of the type that stimulated the formation of antibody that results in the following reaction
Agglutination
Precipitation
Complement fixation
Phagocytosis
Neutralization of an exotoxin
Opsonization
Tissue fixation
Chemotaxis
Activation of mast cells and basophils
PPT prepared by:
DR.PRINCE C P
Associate Professor , Department of Microbiology,
Mother Theresa Post Graduate & Research Institute of Health Sciences (Government of Puducherry Institution)
Serological tests with labelled antibody theoretical questionsEneutron
This document provides information on various serological tests that use labelled antibodies, including immunofluorescence, radioimmune assay (RIA), enzyme-linked immune sorbent assay (ELISA), and immunoblotting. It discusses the basic principles, reagents, procedures, and applications of each technique. It also outlines practical activities for students to perform ELISA to detect antibodies in test sera and draw diagrams of ELISA, immunoblotting, and immunofluorescence testing.
The document provides an overview of ELISA (enzyme-linked immunosorbent assay), a biochemical technique used to detect antibodies or antigens in a sample. It defines key terms like antibodies, antigens, analytes, and describes the main ELISA techniques: competitive ELISA, sandwich ELISA, and indirect ELISA. Competitive ELISA involves competition between labeled and unlabeled antigens for antibody binding sites. Sandwich ELISA detects antigens by coating the plate with capture antibodies and using enzyme-linked detection antibodies. Indirect ELISA detects antibodies by coating the plate with antigens and using enzyme-linked secondary antibodies.
The document discusses ELISA (enzyme-linked immunosorbent assay), a commonly used analytical technique for detecting antigens, antibodies, and proteins. It describes the basic principles of ELISA, including immobilizing an antigen and detecting it using an enzyme-linked antibody. There are four main types of ELISA discussed: direct ELISA, indirect ELISA, sandwich ELISA, and competitive ELISA. Sandwich ELISA is most common and involves capturing the antigen between two antibodies, followed by detection with enzyme-linked secondary antibodies.
1. ELISA is an immunoassay technique used to detect the presence of antigens or antibodies in samples. It relies on antibodies and enzyme-linked detection to provide sensitive, quantitative results.
2. There are several types of ELISA including direct, indirect, sandwich, and competitive, which differ in their use of capture and detection antibodies.
3. The ELISA procedure involves immobilizing an antibody on a plate, adding samples and detection reagents, washing away unbound material, and measuring enzyme activity to determine antigen or antibody levels.
ELISA (enzyme-linked immunosorbent assay) is a biochemical technique used to detect the presence of an antibody or antigen in a sample. It uses antibodies and color change to identify a substance. The basic principle involves using an enzyme to detect antigen-antibody binding through the conversion of a colorless substrate to a colored product. There are four main types of ELISA: indirect, direct, sandwich, and competitive. ELISA is a sensitive, specific, and widely used technique with applications in detecting serum antibodies, potential allergens, disease outbreaks, antigens, and past exposure to diseases.
1. ELISA (Enzyme-linked immunosorbent assay) is an immunoassay technique used to detect antibodies, proteins, peptides, and other molecules. It relies on an antigen-antibody reaction to detect the presence of a substance.
2. The document provides detailed information on the basic principles and steps of ELISA, including coating a plate with antibodies, adding samples and reagents, washing steps, and detecting reactions using enzymes and substrates.
3. Key aspects of performing ELISA are discussed such as sample treatment and storage, controlling humidity and air flow during incubations, and troubleshooting poor results. Direct, indirect, sandwich, and competitive ELISA techniques are also summarized.
Test for detection of plant virus by ELISA test.pdfLOKESH R
This presentation provides an overview of the Enzyme-Linked Immunosorbent Assay (ELISA) test for the detection of plant viruses. The ELISA test is a highly sensitive and specific method for detecting viral antigens in plant tissues, seeds, and other plant materials.
The presentation covers the principles of ELISA test, including the different types of ELISA tests, such as direct, indirect, sandwich, and competitive ELISA. The steps involved in the ELISA test, including sample preparation, antibody labeling, incubation, and detection, are discussed in detail.
The advantages and limitations of the ELISA test for plant virus detection are also highlighted. The presentation includes a comparison of the ELISA test with other diagnostic methods for plant viruses, such as polymerase chain reaction (PCR) and serological assays.
Finally, the presentation provides examples of the applications of the ELISA test for plant virus detection, including its use in crop protection, disease management, and quarantine measures. The presentation concludes with a summary of the key points discussed and recommendations for the use of the ELISA test in plant virus detection.
ELISA (Enzyme-Linked Immunosorbent Assay) is a common laboratory technique used to detect the presence and quantify concentrations of antigens, antibodies, and other proteins in biological samples. It relies on the principle of antigen-antibody binding and uses an enzyme-linked secondary antibody for detection. There are different types of ELISA depending on the detection method and procedure, including direct, indirect, and sandwich ELISA. ELISA has many applications, such as disease diagnosis, food allergen testing, and measuring antibody levels after infection or vaccination.
ELISA (enzyme-linked immunosorbent assay) is a commonly used laboratory technique for detecting antibodies or antigens in a sample. It involves immobilizing an antigen or antibody on a plate and detecting it using an enzyme-linked antibody or antigen. There are four main types of ELISA: direct, indirect, sandwich, and competitive. The document provides detailed descriptions of each ELISA type and discusses the principles, components, applications, advantages, and troubleshooting of ELISA testing.
This document provides information on various immunofluorescence and ELISA techniques. It discusses:
1. Direct and indirect immunofluorescence techniques where fluorescent dyes are used to label antibodies or antigens and examined under fluorescence microscopy.
2. The principles and procedures of different ELISA techniques including indirect ELISA using enzyme-labeled detection antibodies, sandwich ELISA using two antibodies to capture antigens, and competitive ELISA using competition between unlabeled and enzyme-labeled antigens.
3. Examples of applications like detecting pathogens in tissues, phosphorylated proteins in cells, and methanotrophic bacteria using immunofluorescence. ELISA is described as a sensitive assay for quantitatively analyzing proteins and clinical samples.
serological techniques for detection of plant virus.pptxReddykumarAv
Serological tests involve diagnostic procedures for identifying antibodies and antigens in a patient's blood sample. Serology definition tells that Serological tests could be used to diagnose infections and autoimmune disorders, as well as to see whether a person is resistant to these kinds of diseases and for a variety of other purposes, including assessing a person's blood type.
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This presentation explains about the principle and procedure involved in elisa method of immunoassay, development o f elisa , application advantages and disadvantages of elisa
ELISA, or enzyme-linked immunosorbent assay, is an immunoassay technique used to detect the presence of antibodies or antigens in a biological sample. It involves an enzymatic reaction that produces a detectable color change if the target antibody or antigen is present. There are several types of ELISA including direct, indirect, sandwich, and competitive ELISA. ELISA tests are commonly used to screen blood donations, detect infections, measure hormone and toxin levels, and detect allergens and drugs.
ELISA involves an antigen-antibody reaction between a solid surface like a microwell plate coated with the target antigen and an enzyme-labeled antibody specific to that antigen. There are four main types of ELISA - direct, indirect, sandwich, and competitive - which differ in the order and number of antibodies used. ELISA is commonly used to detect antigens and antibodies and has applications in fields like medical diagnostics, food testing, and environmental monitoring.
Radioimmunoassay (RIA) is a sensitive technique introduced in 1960 that uses radioactive isotopes and antibodies to measure antigens or hormones. It involves competitive binding between radiolabeled and unlabeled antigens for antibodies. Enzyme-linked immunosorbent assay (ELISA) is a common technique introduced in 1971 that uses enzymes to detect and quantify substances like proteins. It can be indirect, sandwich, or competitive based on antigen-antibody binding structure. Both RIA and ELISA are sensitive techniques used to detect substances like hormones, vitamins, drugs, and antigens in clinical medicine and research.
ELISA (Enzyme-Linked Immunosorbent Assay) is a biochemical technique used to detect antibodies or antigens in a sample. There are three main types of ELISA: competitive ELISA where a labeled antigen competes for antibody binding sites; sandwich ELISA where antibodies coat a surface to detect a specific antigen; and indirect ELISA where a test antigen is coated on a surface and secondary antibodies are used to detect primary antibodies in a serum sample. The amount of color change produced corresponds to the concentration of antigen or antibody in the original sample.
The document provides an overview of enzyme-linked immunosorbent assay (ELISA), including its history, principles, requirements, types (direct, indirect, sandwich, competitive), applications (ELISPOT), equipment (washer, reader), and generations used for HIV detection. ELISA is a sensitive technique for detecting antigen-antibody interactions using an enzyme-substrate system for detection. It was developed in 1971 and involves multiple steps with different reagents to separate bound and unbound fractions.
elisaLecture_based on new way to medical lab.pptmainakg09
The document describes the enzyme-linked immunosorbent assay (ELISA) technique. ELISA uses antibodies and antigen-enzyme conjugates to detect the presence and quantify antigens or antibodies in a sample. It is a sensitive, specific immunoassay that is usually performed in microwell plates, where antibodies or antigens bind to the wells and are detected using enzyme-linked antibodies and color-changing substrates. There are different types of ELISA including sandwich, competitive, and indirect formats.
Antigen ,Antibody and Ag-Ab reactions ppt by DR.C.P.PRINCEDR.PRINCE C P
An immunogen refers to a molecule that is capable of eliciting an immune response, whereas an antigen refers to a molecule that is capable of binding to the product of that immune response (Ab).
So, an immunogen is necessarily an antigen, but an antigen may not necessarily be an immunogen
The terms immunogen and antigen are often used interchangeably but the later is more common.
Antibodies are Globulin Protein (Immunoglobulin) that are synthesized in the Serum and Tissue fluids.
It reacts specifically with the antigen that stimulated their production.
There are two types serum proteins: albumin and globulin
There are Three types of globulins .
1. Alpha globulin
2. Beta globulin
3. Gamma globulin (Antibodies)
Gamma globulins are responsible for immunity. So they are called as Immunoglobulin (Ig)
The binding of an antibody with an antigen of the type that stimulated the formation of antibody that results in the following reaction
Agglutination
Precipitation
Complement fixation
Phagocytosis
Neutralization of an exotoxin
Opsonization
Tissue fixation
Chemotaxis
Activation of mast cells and basophils
PPT prepared by:
DR.PRINCE C P
Associate Professor , Department of Microbiology,
Mother Theresa Post Graduate & Research Institute of Health Sciences (Government of Puducherry Institution)
Serological tests with labelled antibody theoretical questionsEneutron
This document provides information on various serological tests that use labelled antibodies, including immunofluorescence, radioimmune assay (RIA), enzyme-linked immune sorbent assay (ELISA), and immunoblotting. It discusses the basic principles, reagents, procedures, and applications of each technique. It also outlines practical activities for students to perform ELISA to detect antibodies in test sera and draw diagrams of ELISA, immunoblotting, and immunofluorescence testing.
The document provides an overview of ELISA (enzyme-linked immunosorbent assay), a biochemical technique used to detect antibodies or antigens in a sample. It defines key terms like antibodies, antigens, analytes, and describes the main ELISA techniques: competitive ELISA, sandwich ELISA, and indirect ELISA. Competitive ELISA involves competition between labeled and unlabeled antigens for antibody binding sites. Sandwich ELISA detects antigens by coating the plate with capture antibodies and using enzyme-linked detection antibodies. Indirect ELISA detects antibodies by coating the plate with antigens and using enzyme-linked secondary antibodies.
The document discusses ELISA (enzyme-linked immunosorbent assay), a commonly used analytical technique for detecting antigens, antibodies, and proteins. It describes the basic principles of ELISA, including immobilizing an antigen and detecting it using an enzyme-linked antibody. There are four main types of ELISA discussed: direct ELISA, indirect ELISA, sandwich ELISA, and competitive ELISA. Sandwich ELISA is most common and involves capturing the antigen between two antibodies, followed by detection with enzyme-linked secondary antibodies.
1. ELISA is an immunoassay technique used to detect the presence of antigens or antibodies in samples. It relies on antibodies and enzyme-linked detection to provide sensitive, quantitative results.
2. There are several types of ELISA including direct, indirect, sandwich, and competitive, which differ in their use of capture and detection antibodies.
3. The ELISA procedure involves immobilizing an antibody on a plate, adding samples and detection reagents, washing away unbound material, and measuring enzyme activity to determine antigen or antibody levels.
ELISA (enzyme-linked immunosorbent assay) is a biochemical technique used to detect the presence of an antibody or antigen in a sample. It uses antibodies and color change to identify a substance. The basic principle involves using an enzyme to detect antigen-antibody binding through the conversion of a colorless substrate to a colored product. There are four main types of ELISA: indirect, direct, sandwich, and competitive. ELISA is a sensitive, specific, and widely used technique with applications in detecting serum antibodies, potential allergens, disease outbreaks, antigens, and past exposure to diseases.
1. ELISA (Enzyme-linked immunosorbent assay) is an immunoassay technique used to detect antibodies, proteins, peptides, and other molecules. It relies on an antigen-antibody reaction to detect the presence of a substance.
2. The document provides detailed information on the basic principles and steps of ELISA, including coating a plate with antibodies, adding samples and reagents, washing steps, and detecting reactions using enzymes and substrates.
3. Key aspects of performing ELISA are discussed such as sample treatment and storage, controlling humidity and air flow during incubations, and troubleshooting poor results. Direct, indirect, sandwich, and competitive ELISA techniques are also summarized.
Test for detection of plant virus by ELISA test.pdfLOKESH R
This presentation provides an overview of the Enzyme-Linked Immunosorbent Assay (ELISA) test for the detection of plant viruses. The ELISA test is a highly sensitive and specific method for detecting viral antigens in plant tissues, seeds, and other plant materials.
The presentation covers the principles of ELISA test, including the different types of ELISA tests, such as direct, indirect, sandwich, and competitive ELISA. The steps involved in the ELISA test, including sample preparation, antibody labeling, incubation, and detection, are discussed in detail.
The advantages and limitations of the ELISA test for plant virus detection are also highlighted. The presentation includes a comparison of the ELISA test with other diagnostic methods for plant viruses, such as polymerase chain reaction (PCR) and serological assays.
Finally, the presentation provides examples of the applications of the ELISA test for plant virus detection, including its use in crop protection, disease management, and quarantine measures. The presentation concludes with a summary of the key points discussed and recommendations for the use of the ELISA test in plant virus detection.
ELISA (Enzyme-Linked Immunosorbent Assay) is a common laboratory technique used to detect the presence and quantify concentrations of antigens, antibodies, and other proteins in biological samples. It relies on the principle of antigen-antibody binding and uses an enzyme-linked secondary antibody for detection. There are different types of ELISA depending on the detection method and procedure, including direct, indirect, and sandwich ELISA. ELISA has many applications, such as disease diagnosis, food allergen testing, and measuring antibody levels after infection or vaccination.
ELISA (enzyme-linked immunosorbent assay) is a commonly used laboratory technique for detecting antibodies or antigens in a sample. It involves immobilizing an antigen or antibody on a plate and detecting it using an enzyme-linked antibody or antigen. There are four main types of ELISA: direct, indirect, sandwich, and competitive. The document provides detailed descriptions of each ELISA type and discusses the principles, components, applications, advantages, and troubleshooting of ELISA testing.
This document provides information on various immunofluorescence and ELISA techniques. It discusses:
1. Direct and indirect immunofluorescence techniques where fluorescent dyes are used to label antibodies or antigens and examined under fluorescence microscopy.
2. The principles and procedures of different ELISA techniques including indirect ELISA using enzyme-labeled detection antibodies, sandwich ELISA using two antibodies to capture antigens, and competitive ELISA using competition between unlabeled and enzyme-labeled antigens.
3. Examples of applications like detecting pathogens in tissues, phosphorylated proteins in cells, and methanotrophic bacteria using immunofluorescence. ELISA is described as a sensitive assay for quantitatively analyzing proteins and clinical samples.
serological techniques for detection of plant virus.pptxReddykumarAv
Serological tests involve diagnostic procedures for identifying antibodies and antigens in a patient's blood sample. Serology definition tells that Serological tests could be used to diagnose infections and autoimmune disorders, as well as to see whether a person is resistant to these kinds of diseases and for a variety of other purposes, including assessing a person's blood type.
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Adhd Medication Shortage Uk - trinexpharmacy.comreignlana06
The UK is currently facing a Adhd Medication Shortage Uk, which has left many patients and their families grappling with uncertainty and frustration. ADHD, or Attention Deficit Hyperactivity Disorder, is a chronic condition that requires consistent medication to manage effectively. This shortage has highlighted the critical role these medications play in the daily lives of those affected by ADHD. Contact : +1 (747) 209 – 3649 E-mail : sales@trinexpharmacy.com
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotesPsychoTech Services
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
Muktapishti is a traditional Ayurvedic preparation made from Shoditha Mukta (Purified Pearl), is believed to help regulate thyroid function and reduce symptoms of hyperthyroidism due to its cooling and balancing properties. Clinical evidence on its efficacy remains limited, necessitating further research to validate its therapeutic benefits.
Histololgy of Female Reproductive System.pptxAyeshaZaid1
Dive into an in-depth exploration of the histological structure of female reproductive system with this comprehensive lecture. Presented by Dr. Ayesha Irfan, Assistant Professor of Anatomy, this presentation covers the Gross anatomy and functional histology of the female reproductive organs. Ideal for students, educators, and anyone interested in medical science, this lecture provides clear explanations, detailed diagrams, and valuable insights into female reproductive system. Enhance your knowledge and understanding of this essential aspect of human biology.
- Video recording of this lecture in English language: https://youtu.be/kqbnxVAZs-0
- Video recording of this lecture in Arabic language: https://youtu.be/SINlygW1Mpc
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
These lecture slides, by Dr Sidra Arshad, offer a simplified look into the mechanisms involved in the regulation of respiration:
Learning objectives:
1. Describe the organisation of respiratory center
2. Describe the nervous control of inspiration and respiratory rhythm
3. Describe the functions of the dorsal and respiratory groups of neurons
4. Describe the influences of the Pneumotaxic and Apneustic centers
5. Explain the role of Hering-Breur inflation reflex in regulation of inspiration
6. Explain the role of central chemoreceptors in regulation of respiration
7. Explain the role of peripheral chemoreceptors in regulation of respiration
8. Explain the regulation of respiration during exercise
9. Integrate the respiratory regulatory mechanisms
10. Describe the Cheyne-Stokes breathing
Study Resources:
1. Chapter 42, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 36, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 13, Human Physiology by Lauralee Sherwood, 9th edition
- Video recording of this lecture in English language: https://youtu.be/Pt1nA32sdHQ
- Video recording of this lecture in Arabic language: https://youtu.be/uFdc9F0rlP0
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...Oleg Kshivets
Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
2. ELISA(Enzyme linked immunosorbent assay)
Is one of the immunoassay method used to
detection of 1-ANTIBODIES
2 PROTEINS
3 PEPTIDES
4 BIOMOLECULES
3. The term “immunoassay” is a
combined term of
“immuno”=(practically antigen-
antibody reaction) and
“Assay”=(Determination of purity of
substance or the amount of any
constituent of a mixture.
4. Enzyme Linked ImmunosorbentAssay
1.Antigen/Antibody of interest is absorbed on to plastic
surface (‘Sorbent’)
2.Antigen is recognised by specific antibody (‘Immuno’)
3.This antibody recognised by second antibody
(‘immuno’) which has enzyme attached(‘enzyme
linked’)
4.Substrate react with enzyme to produce product usually
coloured.
5. Radioimmunoassay was first described in a scientific
paper by Rosalyn Sussman Yalow and Solomon
Berson Published in 1960.
In 1971, Peter perlmann and Eva Engvall stockhlom
University in sweeden,and anton schuurs and Bauke
van Wccmen in the Netherlands independently
published papers that synthesized this knowledge into
methods to perform EIA/ELISA.
7. Antibody:proteins produced by the immune system
which help defend against antigens
Symbol for
antibody
The variable regions are thoughTo be the place for
recognition and binding with antigen.
8. • Any molecules that induces production of antibodies
when introduced in the body is called antigen,
OR
• Any “thing” foreign to the immune system eg.bacteria
viruses,(or their parts), pollen..etc.
9. in immunoassay, it is necessary to use any marker to
know the antigen-antibody binding.for such purpose,
we label either antigen or antibody with some
materials that do not interfere with the binding.
eg.Horse radish peroxidase
ezyme Substrate
:trimethylbenzidine
12. use an enzyme to detect the binding of antigen(Ag)
antibody(Ab)
The enzyme convert a colourless substrate
(chromogen)to coloured product,indicating the
presence of Ag-Ab binding.
An elisa can be used to detect either the presence of
antigen or antibodies in a sample depending how the
test is designed
Elisa was developed in 1970 and become rapidly
accepted
13.
14. Qualitative
determines antigen or antibody is present or absent
Quantitative
o determine the quantity of antibody
o titer
o the highest dilution of the specimen usually
serum which gives positive reaction in the test
15.
16. Reagents are relatively cheap and have long shelf life
ELISA is highly specific and sensitive
No radiations hazards occur during labelling or
disposal of waste.
Easy to perform and quick procedures
Equipment can be inexpensive and widely available.
ELISA can be used to variety of infections.
17. Measurement of enzyme activity can be more
complex than measurement of activity of some type of
radioisotopes.
Enzyme activity may be affected by plasma
constituent.
Kits are commercially available ,but not cheap
Very specific to a particular antigen. Won’t recognize
any other antigen.
False positive/negative possible, especially with
mutated/altered antigen
19. Initially the substrate should be colourless
After degradation by enzyme it should be strongly
coloured or flurescent.
ENZYME SUBSTRATE CHROMOGEN STOPPING
Alkaline phosphatase P-NPP P-
NPP+diethandamine+
MgCl2
1M NaOH
Horse radish peroxide H2O2 Tetramethylbenzidine
+phosphate citrate
buffer
1M H2SO4
Horse radish peroxide H2O2 O-phenylenediamine+
HCL
1M HCL
20. 1.Collection and processing of serum
2.Coating of wells antibodies/antigens
3.Washing
4.Incubation with the test sample
5.Washing
6.Incubation with enzyme conjugated antibodies
7.Washing again
8.Colour development by adding chromogenic substrate
9.Stopping the colour development
10.Reading of results
21.
22. Collect blood in a tubes that does not
contain any chemicals or
anticoagulants.
Collect 5ml of whole blood(for every small
children collect 1ml).
Place tube upright 30-60 minutes then
when firm clot has formed centrifuge tube
for 20 minutes at 2500rpm.
Remove serum with the pipette and place
in a plastic storage tube(2-3,microtube or
cryovial).
If 5ml of blood was collected it will result in
23. Antigen: Chicken gamma globulin
Primary antibody (PA): Polyclonal anti-chicken
antibody made by rabbits
Secondary antibody (enzyme-linked) SA: Polyclonal
anti-rabbit antibody made by goats linked
(conjugated) to horseradish peroxidase (HRP)
Enzyme substrate (SUB): 3,3’,5,5’ –
tetramethylbenzidine (TMB) – a colorless solution
that when oxidized by HRP turns blue
24. Using a clean pipette, add 100microliter of diluted serum sample
(diluted the sample to be tested 1:100 in sample diluent)to each
well.
Incubate 1hr at 37 degree celcius
After incubation empty out contents of wells into waste
container.
Using pipette ,fill wells with the washing buffer the empty out.
Tap wells upside down o paper towel.
Wash the well 5 times. At the end of washing process the well
must be entirely dry after the last wash.
Distribute 100microliter of antihuman Immunoglobuline
conjugate in each well. incubate 30 minutes at 37 degree celcius.
25. Primary Antibody Secondary Antibody
Raised against specific antigen.
Typically unconjugated (Unlabelled)
Bind to primary antibody.
Conjugated to probes (Labelled)
Recognize and bind with high affinity
and specificity to unique epitopes .
Available as monoclonal and/or
polyclonal antibody.
Specificity for whole Ig molecules or
antibody fragments. Available as
monoclonal or ployclonal antibody.
Specific to protein of interest Specific to primary antibody and no
affinity to protein of interest
Anti-bovine Tubulin antibody is a
primary antibody that will recognize
the tubulin protein that is generated
in the cow (bovine).
Goat anti-mouse IgG antibody is a
secondary antibody developed in the
goat that has an affinity for the
mouse IgG protein.
26.
27. Monoclonal
Antibody
Polyclonal
Antibody
Consists of one antibody
class/subclass which is selective for a
single epitope on the antigen
Contains a mixture of antibodies
(mainly IgGs), often recognizing
multiple epitopes on the antigen
Usually generated in mice, other
rodents, or isolated from a phage
display library
Generated in a variety of species
including rabbit, goat, sheep, and
donkey
Always identical, as they are produced
from the same hybridoma (or
transfected cell line, or bacterial
clone)
Prone to batch to batch variability
Their homogeneous nature ensures
better reproducibility between tests,
where conditions are kept constant.
Useful for quantification experiments
like flow cytometry
Their heterogeneity means they are
more tolerant to slight changes to
the antigen e.g. denaturation,
polymorphism or differences
in glycosylation state
28. Monoclonal
Antibody
Polyclonal
Antibody
Because of their specificity, they
are less likely to cross-react with
other proteins, giving
lower background than polyclonal
antibodies
May contain non-specific
antibodies resulting in
background staining
Specificity makes them ideal as the
primary antibody in an assay, for
detecting antigens in tissue, or for
affinity purification of antigens
Useful as secondary antibodies
or for immunoprecipitation, as
they target multiple epitopes
providing a more robust
detection
34. Blood >> processed Serum >> applied to ELISA plate containing antigen >>
Wash >> enzyme >> colour change.
Accuracy :False positive for person not HIV infected. It is possible in peoples
having antibodies for Human leukocyte Antigen (HLA). Observed in
women who possess multiple pregnancies.
After 4-8 weeks of exposure to the HIV virus, the body will have produced
a detectable level antibodies (immune response) against HIV
35.
36. 0 5 10 15 20
Plant pathology
Autoimmune
Food safety/tolerance
Enzymes
Allergy
Cancer
Inflammation
Protein:Protein interactions
Other
Endocrinology
% Responding
% Responding
Main application areas for respondent’s ELISA
37. 0 20 40 60 80
Serum
Plasma
Cultured cells
Whole blood
Cell lysate
Tissue homogenate
Urine
Cancer/tumour cells
Saliva
Other bodily fluids
Other
Plant material
Biopsy
Faeces
Foodstuff
Environmental sample
Dried Blood Spot
Water
No Responding (All
Respondents)
Sample sources analysed By ELISA