provides the brief explanation on how the elastin and fibronectin and basal lamina are present in our body

1. Elastin,Fibronectin and Basal
lamina
What Are these exactly?

2. Elastin
• Of course as the name suggests the elastin is
elastic???
• Elastin is made up of glycine, valine, alanine,
and proline Residues
• As we are Biotechnologists we may have to
surely Know which the gene is responsible??
ELN gene at 7th chromosome has the
information on ELASTIN

3. Elastin
• Elastin is a key protein of the extracellular
matrix.
• It is highly elastic and present in connective
tissue allowing many tissues in the body to
resume their shape after stretching or
contracting.
• Molecular weight : 552.7 g/mol
• Molecular Formula : C27H48N6O6

4. How it Look like???

5. Relax or unstreched
Stressed

7. Fibronectin
• Fibronectin is a glycoprotein
• Fibronectin is a high-molecular weight protein
(~440kDa)
• Specific domains allow fibronectin to
promote cell-to-cell adhesion, cell-to-basement-
membrane attachment, clot stabilization,
embryogenesis, nerve regeneration, fibroblast
migration, macrophage function, and pathogen
(virus, fungus, bacteria, and protozoa) binding to
mammalian cells and extracellular matrix.

8. They are Tissue Specific!!!
• Different forms of fibronectin appear to be
generated from tissue specific splicing of
fibronectin mRNA, transcribed from a single
gene.
• FN1 gene holds the info to synthesize the
Fibronectin

9. Chromosomal position

10. What does it do ??? And Where is it
• Fibronectin sometimes serves as a
general cell adhesion molecule by anchoring
cells to collagen or proteoglycan substrates
• Soluble plasma fibronectin is produced by
hepatocytes in the liver and is a major
component of blood plasma. Insoluble
cellular fibronectin is secreted by many cells
in the body

12. Basal lamina
• Functions of basal lamina:
• Support
• Selective barrier
• Influencing cell polarity distinguish the basal
& apical sides.
• Regulation of proliferation and differentiation
• Affect cell-cell interaction
• Pathway for cell migration

13. BASAL LAMINA :Layers of basal lamina:
Lamina Lucida (rara) Lamina Densa

14. Basal lamina
Reticular lamina