The document discusses elastin, fibronectin, and basal lamina, highlighting their structure, function, and genetic information associated with their synthesis. Elastin is an elastic protein critical for connective tissue integrity, while fibronectin is a glycoprotein that facilitates various cell functions. The basal lamina serves as a supportive and regulatory structure for cell behavior and interaction.

1. Elastin,Fibronectin and Basal
lamina
What Are these exactly?

2. Elastin
• Of course as the name suggests the elastin is
elastic???
• Elastin is made up of glycine, valine, alanine,
and proline Residues
• As we are Biotechnologists we may have to
surely Know which the gene is responsible??
ELN gene at 7th chromosome has the
information on ELASTIN

3. Elastin
• Elastin is a key protein of the extracellular
matrix.
• It is highly elastic and present in connective
tissue allowing many tissues in the body to
resume their shape after stretching or
contracting.
• Molecular weight : 552.7 g/mol
• Molecular Formula : C27H48N6O6

4. How it Look like???

5. Relax or unstreched
Stressed

7. Fibronectin
• Fibronectin is a glycoprotein
• Fibronectin is a high-molecular weight protein
(~440kDa)
• Specific domains allow fibronectin to
promote cell-to-cell adhesion, cell-to-basement-
membrane attachment, clot stabilization,
embryogenesis, nerve regeneration, fibroblast
migration, macrophage function, and pathogen
(virus, fungus, bacteria, and protozoa) binding to
mammalian cells and extracellular matrix.

8. They are Tissue Specific!!!
• Different forms of fibronectin appear to be
generated from tissue specific splicing of
fibronectin mRNA, transcribed from a single
gene.
• FN1 gene holds the info to synthesize the
Fibronectin

9. Chromosomal position

10. What does it do ??? And Where is it
• Fibronectin sometimes serves as a
general cell adhesion molecule by anchoring
cells to collagen or proteoglycan substrates
• Soluble plasma fibronectin is produced by
hepatocytes in the liver and is a major
component of blood plasma. Insoluble
cellular fibronectin is secreted by many cells
in the body

12. Basal lamina
• Functions of basal lamina:
• Support
• Selective barrier
• Influencing cell polarity distinguish the basal
& apical sides.
• Regulation of proliferation and differentiation
• Affect cell-cell interaction
• Pathway for cell migration

13. BASAL LAMINA :Layers of basal lamina:
Lamina Lucida (rara) Lamina Densa

14. Basal lamina
Reticular lamina