Bioprocess Technology - Product purification, polishing and packaging.

1. Downstream processing
S. Sivaranjani Arunnehru
Assistant Professor
Dept. of Biotechnology
Bon Secours College for Women
Thanjavur

4. Solid-liquid separation
• Floatation
• Flocculation
– Filters
– Depth filters
– Absolute filters
– Rotary drum vaccum filters
– Membrane filters
» Types
• Microfiltration – 0.1 to 10 µm
• Ultrafiltration – 0.001 to 0.1 µm
• Reverse Osmosis – 0.0001 to 0.001 µm

9. • Centrifugation:
– Types:
• Tubular bowl centrifuge
• Disc centrifuge
• Multichamber centrifuge
• Scroll centrifuge

11. 1.Ultrasonication 1. Alkali 1. Lysosyme
2. Osmotic Shock 2. Organic solvents 2. Glucanase
3. Heat shock 3. Detergents 3. Mannase & Protease
4. High Pressure Homogenization
5. Impingement
6. Grinding with glass beads
• Release of Intracellular products:
Cell disruption
Physical
Chemical
Enzymatic

13. • Concentration
• Evaporation
– Plate evaporators
– Falling film
– Forced film
– Liquid – liquid Extraction
» Extraction of low molecular weight products
» Extraction of high molecular weight products
• Membrane filtration
• Precipitation
• Adsorption

15. • Purification
– Chromatography
• Gel filtration
• Ion exchange
• Chromatofocussing
• Affinity
• Hydrophobic
• Immobilized metal ion affinity

17. • Formulation
• Drying
– Types
• Spray drying
• Freeze drying
• Final product