The document is a model question paper for DMLT-III Biochemistry, comprising questions divided into two sections: short answer questions worth 40 marks and detailed answer questions worth 60 marks. Topics covered include auto analyzers, blood glucose regulation, quality control in laboratories, various biochemical tests, and the principles of different analytical techniques. It serves as a comprehensive assessment tool for students in the field of clinical biochemistry.

1. ISMAIL TAHASILDAR (M.Sc.B.ed.DNHE) Page 1
BIOCHEMISTRY LECTURER
DISTRICTHOSPITALDHARWAD.
DHARWAD INSTITUTE OF PARAMEDICAL SCIENCE,
DISTRICT HOSPITAL DHARWAD.
MODEL QUESTION PAPER-01
Class: DMLT-III BIOCHEMISTRY Marks: 100
Q.No: Answer the following Questions 5X8=40
01. Write detail role of Auto analyser in clinical Biochemistry.
02. Write in detail about the regulation of blood glucose.
03. What is Quality control? Explain internal & external quality control.
04. Different methods of Glucose Estimation, Principle, advantage and disadvantage.
05. Write in detail about Electrophoresis Principle, Types & Applications.
06. Write in detail about colorimeter, Principle, advantage and disadvantage
07. Jaundice, its types and their biochemical findings.
08. Renal function test.
Q.No: Answer the following Questions 3X20=60
10. Liver function tests.
11. Spectrophotometer.
12. Abnormal constituents of urine.
13. Specific gravity of Urine.
14. Vandenberg’s test.
15. Glucose tolerance test.
16. ELISA.
17. Rothera’s test.
18. Metabolic acidosis.
19. Accuracy, Precision, Specificity, Sensitivity.
20. Reference values and Interpretations.
21. Ketone bodies.
22. Cardiac profile tests.
23. Flame photometry.
24. Batch analyser.
25. Lipid profile.
26. Principles of RIA
27. Reference range
28. Synthesis of creatinine
29. Pre-analytical factors.

2. ISMAIL TAHASILDAR (M.Sc.B.ed.DNHE) Page 2
BIOCHEMISTRY LECTURER
DISTRICTHOSPITALDHARWAD.
DHARWAD INSTITUTE OF PARAMEDICAL SCIENCE,
DISTRICT HOSPITAL DHARWAD.
MODEL QUESTION PAPER-01
Class: DMLT-III BIOCHEMISTRY Marks: 100
Q.No: Answer the following Questions 5X8=40
01. Disturbance in acid Base-Balance.
02. Describe the principles, applications and different types of auto analyser.
03. What is electrophoresis? Write the principle, types and applications of electrophoresis.
04. Normal constituents of urine and detection of the same in a laboratory. Add a note on specific gravity of
Urine.
05. Respiratory acidosis
06. Principles of quality Assurance and Standards for clinical chemistry
07. Radioimmunoassay -Principles & Applications.
08. Different methods of Cholesterol Estimation- Principle, advantage and disadvantage.
Q.No: Answer the following Questions 3X20=60
09. Semi-Autoanalyser.
10. LFT.
11. Normal range of Chlorine, sodium and potassium
12. Clearance test.
13. Clinical importance of blood sugar estimation.
14. Folin wu method
15. Blood gas analyser
16. Name two tests done to detect Myocardial infarction.
17. Random Access Analyzers.
18. Normal range of Total, Direct and Indirect bilirubin
19. Limits of error allowable in laboratory
20. Metabolic alkalosis
21. Urea and creatinine clearance test.
22. Blood buffers and their role in maintenance of pH.
23. Name electrolytes and their normal value.
24. Name the Cardiac Markers.
25. GTT
26. Quality Control in Laboratory.
27. Abnormal constituents of urine.
28. Explain formation of creatinine
29. Enzyme linked immune sorbent assay (ELISA).

3. ISMAIL TAHASILDAR (M.Sc.B.ed.DNHE) Page 3
BIOCHEMISTRY LECTURER
DISTRICTHOSPITALDHARWAD.
DHARWAD INSTITUTE OF PARAMEDICAL SCIENCE,
DISTRICT HOSPITAL DHARWAD.
MODEL QUESTION PAPER-01
Class: DMLT-III BIOCHEMISTRY Marks: 100
Q.No: Answer the following Questions 5X8=40
01. Cardiac enzymes in Heart disease.
02. Jaundice and its classification.
03. Mention different methods of Glucose estimation.
04. Metabolic acidosis & alkalosis.
05. Formation of Bilirubin.
06. Enzyme Linked Immunosorbant Assay (ELISA).
07. Colorimetry, Principle, absorbance, transmittance and applications.
08. Spectrophotometry and its importance.
Q.No: Answer the following Questions 3X20=60
09. Rothera's test
10. Specific gravity of urine
11. Clearance test
12. Normal range of electrolytes
13. Write two uses of Ion selective electrode (ISE)
14. Two applications of electrophoresis
15. Name two enzymes of lipid digestion
16. Normal range of serum urea & serum Creatinine
17. Name the lipid profile tests
18. Myocardial infarction.
19. Different methods of cholesterol estimation.
20. Application of flame Photometer.
21) Principles of Quality assurance.
23) Pre analytical factors importance in clinical chemistry.
24) Accuracy, Specificity & Sensitivity.
25) Reference values and its interpretations.
26) Laws of Photometry.
27) Benedict’s test.
28) Chloride shift.
29) Biuret test.