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University Of Al-Ameed
College Of Dentistry
Lymphocytes NeutrophilsEosinophil Basophil Monocyte
Image of enlarged white blood cells,
which swallows a germ, as well as
the prominent part of the cartilage
LeukemiaNormal blood
a-Hemocytometer counting chamber (Neubauer,s chamber).
b-WBCs counting pipette.
c-Rubber tube with a plastic mouth piece for drawing the fluid into a pipette.
d-Special hemocytometer coverslip
e-Diluting fluid.
f-Microscope.
Apparatus and Reagents
The area of each large corner square is 1 mm2, the depth of the chamber 0.1 mm (from chamber
surface to coverslip), the volume of blood in one square is 1 mm (length) × 1 mm (width) × 0.1 mm
(high) = 0.1 mm3 ,volume of four corner squares = 0.1 mm3 × 4 = 0.4 mm3 .
No. of cells in large corner square = N.
Dilution factor
No. of WBCs/mm3 = Cells counted × ———————
Volume factor
20
No. of WBCs/mm3 = N × __________________ = N × 200
(In one square) 0.1
20
No. of WBCs/mm3 = N × ____________________ = N × 50
(In four squares) 0.4
Calculation
Adult 4,000-11,000 cell/mm3Infants 15,000-20,000 cell/mm3 Children 4,500-13,500 cell/mm3
WikipediaHealthline

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White Blood Cell count (WBC)

  • 2.
  • 3.
  • 4.
  • 5.
  • 6.
  • 8.
  • 9.
  • 10. Image of enlarged white blood cells, which swallows a germ, as well as the prominent part of the cartilage
  • 11.
  • 13.
  • 14. a-Hemocytometer counting chamber (Neubauer,s chamber). b-WBCs counting pipette. c-Rubber tube with a plastic mouth piece for drawing the fluid into a pipette. d-Special hemocytometer coverslip e-Diluting fluid. f-Microscope. Apparatus and Reagents
  • 15.
  • 16.
  • 17. The area of each large corner square is 1 mm2, the depth of the chamber 0.1 mm (from chamber surface to coverslip), the volume of blood in one square is 1 mm (length) × 1 mm (width) × 0.1 mm (high) = 0.1 mm3 ,volume of four corner squares = 0.1 mm3 × 4 = 0.4 mm3 . No. of cells in large corner square = N. Dilution factor No. of WBCs/mm3 = Cells counted × ——————— Volume factor 20 No. of WBCs/mm3 = N × __________________ = N × 200 (In one square) 0.1 20 No. of WBCs/mm3 = N × ____________________ = N × 50 (In four squares) 0.4 Calculation
  • 18. Adult 4,000-11,000 cell/mm3Infants 15,000-20,000 cell/mm3 Children 4,500-13,500 cell/mm3