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Dialysis Lab and Nature of Science
Dialysis Lab

 Take out your lab notebook! 

 If you have not finished your Cell Observations Lab conclusion,
   leave some space for your conclusion and skip to the next page

 Title: Dialysis Lab

 Purpose: To make observations of a dialysis apparatus in order
   to determine how dialysis tubing functions
Dialysis Lab – Materials and Method

Materials and Methods:
-   Corn Syrup
-   Dialysis Tubing
-   500 mL beaker
-   2 rubber bands
-   10mL pipette
-   Water
-   Clamp and Ring Stand
Dialysis Lab - Materials and Method
Dialysis Lab - Materials and Method
Dialysis Lab - Materials and Method
Dialysis Lab - Results

 Write down any observations you see

 Then write down any questions you have about the
   apparatus.
    For Example: Why does ______________ happen?

 From one question you can come up with a hypothesis
    Maybe __________ happens because the pipette has a cotton
      in it.
Dialysis Lab - Results

 Then you can make an experiment around your hypothesis using
   an if … then statement
    If the cotton ball in the pipette causes the liquid in the pipette
     change and we take out the cotton ball then, there will be no
     change in the amount of liquid in the pipette.
    Here are the materials available for your experiment…

 After writing your hypothesis, have an instructor check it and you
   can write method for this experiment
    Be sure to include
      How you will be recording your data
      Only have 1 variable – change only 1 thing
      A control
Gallery Walk

 When everyone is done with their method, we will walk around
   and annotate each group’s method
    Write one thing that you like about the method and one thing
      they can do to make it better
       Things to look for
          Does the method make sense to you? In other words, is it clear?
          Do they have a way to record their data qualitatively and quantitatively?
          Do they only have one variable?
    Make any adjustments necessary and start your new lab.
What was happening?

         Dialysis is a process
         that takes out waste
         and extra water
         from blood.

         It is an artificial
         replacement for
         kidneys.
The dialysis tube is made
of a permeable
membrane that works by
simple diffusion or
passive diffusion.

Passive Diffusion:
Molecules move from an
area of high
concentration to low
concentration until it fills
the space evenly
(equilibrium)
 Passive Diffusion: Molecules move from an area of
   high concentration to low concentration until it fills
   the space evenly
      Ex: Farts, Purfume, Food coloring in water
Cell Membrane

    The cell
    membrane is
    also semi-
    permeable and
    wants to
    balance with the
    water from the
    environment
    outside.
Lipid Bilayer




Lipid Bilayer: A thin membrane
made of 2 layers of phospholipids.
Phospholipids: lipids with polar head (hydrophilic) and non-polar
tail (hydrophobic) that make up the cell membrane.
But Wait!

  If the fatty acid tails on the phospholipid are non-
    polar, how do the water molecules go through?


Aquaporins! Proteins in the cell membrane that have a
tunnel for water to pass through.
Aquaporins! Proteins in the cell membrane that have a tunnel
for water to pass through using passive transport
Types of Transport Proteins

Passive
Transport:
Moving
molecules in and
out of a
membrane
without using
energy. Can only
move from high
to low
concentration
Active Transport:
Moving
molecules in and
out of a
membrane using
energy.

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Dialysis lab

  • 1.  Dialysis Lab and Nature of Science
  • 2. Dialysis Lab  Take out your lab notebook!   If you have not finished your Cell Observations Lab conclusion, leave some space for your conclusion and skip to the next page  Title: Dialysis Lab  Purpose: To make observations of a dialysis apparatus in order to determine how dialysis tubing functions
  • 3. Dialysis Lab – Materials and Method Materials and Methods: - Corn Syrup - Dialysis Tubing - 500 mL beaker - 2 rubber bands - 10mL pipette - Water - Clamp and Ring Stand
  • 4. Dialysis Lab - Materials and Method
  • 5. Dialysis Lab - Materials and Method
  • 6. Dialysis Lab - Materials and Method
  • 7. Dialysis Lab - Results  Write down any observations you see  Then write down any questions you have about the apparatus.  For Example: Why does ______________ happen?  From one question you can come up with a hypothesis  Maybe __________ happens because the pipette has a cotton in it.
  • 8. Dialysis Lab - Results  Then you can make an experiment around your hypothesis using an if … then statement  If the cotton ball in the pipette causes the liquid in the pipette change and we take out the cotton ball then, there will be no change in the amount of liquid in the pipette.  Here are the materials available for your experiment…  After writing your hypothesis, have an instructor check it and you can write method for this experiment  Be sure to include  How you will be recording your data  Only have 1 variable – change only 1 thing  A control
  • 9. Gallery Walk  When everyone is done with their method, we will walk around and annotate each group’s method  Write one thing that you like about the method and one thing they can do to make it better  Things to look for  Does the method make sense to you? In other words, is it clear?  Do they have a way to record their data qualitatively and quantitatively?  Do they only have one variable?  Make any adjustments necessary and start your new lab.
  • 10. What was happening? Dialysis is a process that takes out waste and extra water from blood. It is an artificial replacement for kidneys.
  • 11.
  • 12. The dialysis tube is made of a permeable membrane that works by simple diffusion or passive diffusion. Passive Diffusion: Molecules move from an area of high concentration to low concentration until it fills the space evenly (equilibrium)
  • 13.
  • 14.  Passive Diffusion: Molecules move from an area of high concentration to low concentration until it fills the space evenly Ex: Farts, Purfume, Food coloring in water
  • 15. Cell Membrane The cell membrane is also semi- permeable and wants to balance with the water from the environment outside.
  • 16. Lipid Bilayer Lipid Bilayer: A thin membrane made of 2 layers of phospholipids.
  • 17. Phospholipids: lipids with polar head (hydrophilic) and non-polar tail (hydrophobic) that make up the cell membrane.
  • 18.
  • 19. But Wait!  If the fatty acid tails on the phospholipid are non- polar, how do the water molecules go through? Aquaporins! Proteins in the cell membrane that have a tunnel for water to pass through.
  • 20. Aquaporins! Proteins in the cell membrane that have a tunnel for water to pass through using passive transport
  • 21. Types of Transport Proteins Passive Transport: Moving molecules in and out of a membrane without using energy. Can only move from high to low concentration
  • 22. Active Transport: Moving molecules in and out of a membrane using energy.

Editor's Notes

  1. The cell membrane is made up of a phospholipid bilayer where the lipids are really cliqu-ish and like to cluster together.Water on the outside water on the inside