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1. Introduction
 Application of Chitosan
4. Conclusion
 High Pressure Homogenizer
(HPH, Nano Disperser, ILSHIN AUTOCLAVE)
 In the HPH process, the pressure and the number of passes increases make
viscosity and molecular weight decrease without any acid/alkali solution (Green
Chemistry).
 The FT-IR spectra indicated no obvious modification of chemical structure of
natural polymer before and after HPH treatment.
 HPH has been shown to be a valid tool to reduce molecular weight of natural
polymer (polysaccharides structure).
Depolymerization of Chitosan Using
a High Pressure Homogenizer
Jun Hee Choa, Taeyoung Kima, Yun Hye Yeona, Hyun Hyo Kima
aDepartment of Research & Development, ILSHINAUTOCLAVE CO. #835 Taplipdong, Yuseonggu, Daejeon 305-510,
Korea
2. Experimental
 Experimental
Large Molecular Weight
1 %(w/v) Chitosan Solution
3. Results & Discussion
Shear Force Cavitation
Entanglement
and stretch
Smaller Fragment
High Molecular Weight
Polymer
Depolymerization
Process Method Advantage Disadvantage
Physical
(Radiation)
Ultrasonic • No Waste (Acid/Alkali), No-residues
• Long Time Process
• Process Noise
E-beam, γ-Ray • No Waste (Acid/Alkali), No-residues
• Dangerous Process
• High Cost
Chemical
Acidic
hydrolysis
• High Solubility
• High Efficiency of Depolymerization
• Acid waste water
• Complex Process
Phosphorylation
Sulfation
• Enhancement of Solubility and
Functionality
• Low Efficiency
Biological
Enzymatic
hydrolysis
• Selective Decomposition
• High Efficiency of Depolymerization
• High Cost
• Inefficiency
Problems
High Viscosity
&
Large Molecular
Weight
 Method of Depolymerization
Plunger
Fluid inlet
Fluid outlet
Hydraulic motive cylinder
Plunger seal
Alternating
motion
 High pressure Intensifier
 Nozzle Chamber
• Shear Force
• Cavitation
• Impact Force
Inlet
High Pressure
Pump
Pressure
Gauge
Nozzle
Chamber
High
Energy
Outlet
Inlet Shear &
Cavitation Zone
Impact
Zone Outlet
Inlet
Pressure (bar) 500 1000 1500 2000 2500
Fluid velocity (m/sec) 313 442 542 626 700
Inlet
Outlet
Outlet
Impact
Zone
Shear &
Cavitation Zone
Shear &
Cavitation Zone
Impact
Zone
 Fluid Velocity (Sound Speed: 340 m/s)
Low Molecular Weight
1 %(w/v) Chitosan Solution
- Natural Polymer: Chitosan
- Solvent: D.I Water
- Nano Disperser: NLM 100
Operating Pressure (bar) 200 – 1500
Max. Flow Rate (ml/min) 100
Pump System Motor Driven
Dimension (mm) 300*450*300
Weight (kg) 30
 Viscosity(1 %(w/v) Chitosan)  Molecular Weight  FT-IR Spectrum
• HPH treatment produces significant changes in the viscosity and molecular weight of the chitosan solution.
• As the pressure and the number of pass increases, the polymer solution shows a significant decrease of viscosity and molecular weight.
• High Depolymerization Effect, Short Process Time
and No Waste Water (Green Chemistry)
C
O
C
O
CH2OH
NH2 NH2
CH2OH
O OHOH
H
H
H
H
HH
H
H H
H
- Chitosan structure
Cosmetic Food Drug
TextileDrug Delivery
System
Environment
 UV-Vis Spectrum
 XRD
 Characterization
- Viscometer
- Gel Chromatograph
- FT-IR
- UV-Vis
- XRD
• The intensity characteristics of the
peaks tend to decrease with
increasing numbers of passes under
high pressure homogenization.
• A decrease in crystallinity occurs due
to the destruction of the crystal
structure of chitosan as a result of the
cleavage of the glycosyl bonds

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Depolymerization of chitosan using a High Pressure Homogenizer

  • 1. 1. Introduction  Application of Chitosan 4. Conclusion  High Pressure Homogenizer (HPH, Nano Disperser, ILSHIN AUTOCLAVE)  In the HPH process, the pressure and the number of passes increases make viscosity and molecular weight decrease without any acid/alkali solution (Green Chemistry).  The FT-IR spectra indicated no obvious modification of chemical structure of natural polymer before and after HPH treatment.  HPH has been shown to be a valid tool to reduce molecular weight of natural polymer (polysaccharides structure). Depolymerization of Chitosan Using a High Pressure Homogenizer Jun Hee Choa, Taeyoung Kima, Yun Hye Yeona, Hyun Hyo Kima aDepartment of Research & Development, ILSHINAUTOCLAVE CO. #835 Taplipdong, Yuseonggu, Daejeon 305-510, Korea 2. Experimental  Experimental Large Molecular Weight 1 %(w/v) Chitosan Solution 3. Results & Discussion Shear Force Cavitation Entanglement and stretch Smaller Fragment High Molecular Weight Polymer Depolymerization Process Method Advantage Disadvantage Physical (Radiation) Ultrasonic • No Waste (Acid/Alkali), No-residues • Long Time Process • Process Noise E-beam, γ-Ray • No Waste (Acid/Alkali), No-residues • Dangerous Process • High Cost Chemical Acidic hydrolysis • High Solubility • High Efficiency of Depolymerization • Acid waste water • Complex Process Phosphorylation Sulfation • Enhancement of Solubility and Functionality • Low Efficiency Biological Enzymatic hydrolysis • Selective Decomposition • High Efficiency of Depolymerization • High Cost • Inefficiency Problems High Viscosity & Large Molecular Weight  Method of Depolymerization Plunger Fluid inlet Fluid outlet Hydraulic motive cylinder Plunger seal Alternating motion  High pressure Intensifier  Nozzle Chamber • Shear Force • Cavitation • Impact Force Inlet High Pressure Pump Pressure Gauge Nozzle Chamber High Energy Outlet Inlet Shear & Cavitation Zone Impact Zone Outlet Inlet Pressure (bar) 500 1000 1500 2000 2500 Fluid velocity (m/sec) 313 442 542 626 700 Inlet Outlet Outlet Impact Zone Shear & Cavitation Zone Shear & Cavitation Zone Impact Zone  Fluid Velocity (Sound Speed: 340 m/s) Low Molecular Weight 1 %(w/v) Chitosan Solution - Natural Polymer: Chitosan - Solvent: D.I Water - Nano Disperser: NLM 100 Operating Pressure (bar) 200 – 1500 Max. Flow Rate (ml/min) 100 Pump System Motor Driven Dimension (mm) 300*450*300 Weight (kg) 30  Viscosity(1 %(w/v) Chitosan)  Molecular Weight  FT-IR Spectrum • HPH treatment produces significant changes in the viscosity and molecular weight of the chitosan solution. • As the pressure and the number of pass increases, the polymer solution shows a significant decrease of viscosity and molecular weight. • High Depolymerization Effect, Short Process Time and No Waste Water (Green Chemistry) C O C O CH2OH NH2 NH2 CH2OH O OHOH H H H H HH H H H H - Chitosan structure Cosmetic Food Drug TextileDrug Delivery System Environment  UV-Vis Spectrum  XRD  Characterization - Viscometer - Gel Chromatograph - FT-IR - UV-Vis - XRD • The intensity characteristics of the peaks tend to decrease with increasing numbers of passes under high pressure homogenization. • A decrease in crystallinity occurs due to the destruction of the crystal structure of chitosan as a result of the cleavage of the glycosyl bonds