Homogenizer, Homomixer, Nanodisperser, High Pressure Homogenizer, Nono emulsion, nano dispersion, nano liposome, cell rupture, atomization ,Impact, shear force, cavitation, High pressure Homogenizer system, impact, shear force, cavitation,高压分散机，高压均质机，均质机，超高压均质机，纳米均质分散机，高压纳米均质机， 空化， 剪切

1. 1. Introduction
 Application of β-glucan
4. Conclusion
 High Pressure Homogenizer
(HPH, Nano Disperser, ILSHIN AUTOCLAVE)
 In the HPH process, the pressure and the number of passes increases make
viscosity and molecular weight decrease without any acid/alkali solution (Green
Chemistry).
 The FT-IR spectra indicated no obvious modification of chemical structure of
polymer before and after HPH treatment.
 HPH has been shown to be a valid tool to reduce molecular weight of natural
polymer (polysaccharides structure).
Facile depolymerization process of β-glucan
Using High Pressure Homogenizer
Jun Hee Choa, Taeyoung Kima, Yun Hye Yeona, Hyun Hyo Kima
aDepartment of Research & Development, ILSHINAUTOCLAVE CO. #835 Taplipdong, Yuseonggu, Daejeon 305-510,
Korea
2. Experimental
 Experimental Large Molecular Weight
5 %(w/v) β-glucan Solution
3. Results & Discussion
Shear Force Cavitation
Entanglement
and stretch
Smaller Fragment
High Molecular Weight
Polymer
Depolymerization
Process Method Advantage Disadvantage
Physical
(Radiation)
Ultrasonic • No Waste (Acid/Alkali), No-residues
• Long Time Process
• Process Noise
E-beam, γ-Ray • No Waste (Acid/Alkali), No-residues
• Dangerous Process
• High Cost
Chemical
Acidic
hydrolysis
• High Solubility
• High Efficiency of Depolymerization
• Acid waste water
• Complex Process
Phosphorylation
Sulfation
• Enhancement of Solubility and
Functionality
• Low Efficiency
Biological
Enzymatic
hydrolysis
• Selective Decomposition
• High Efficiency of Depolymerization
• High Cost
• Inefficiency
Problems
High Viscosity
&
Large Molecular
Weight
 Method of Depolymerization
Plunger
Fluid inlet
Fluid outlet
Hydraulic motive cylinder
Plunger seal
Alternating
motion
 High pressure Intensifier
 Nozzle Chamber
• Shear Force
• Cavitation
• Impact Force
Inlet
High Pressure
Pump
Pressure
Gauge
Nozzle
Chamber
High
Energy
Outlet
Inlet Shear &
Cavitation Zone
Impact
Zone Outlet
Inlet
Pressure (bar) 500 1000 1500 2000 2500
Fluid velocity (m/sec) 313 442 542 626 700
Inlet
Outlet
Outlet
Impact
Zone
Shear &
Cavitation Zone
Shear &
Cavitation Zone
Impact
Zone
 Fluid Velocity (Sound Speed: 340 m/s)
Low Molecular Weight
5 %(w/v) β-glucan Solution
- Natural Polymer: β-glucan
- Solvent: D.I Water
- Nano Disperser: NLM 100
Operating Pressure (bar) 200 – 1500
Max. Flow Rate (ml/min) 100
Pump System Motor Driven
Dimension (mm) 300*450*300
Weight (kg) 30
 Viscosity(5 %(w/v) β-glucan)  Molecular Weight  FT-IR Spectrum
• HPH treatment produces significant changes in the viscosity and molecular weight of the β-glucan solution.
• As the pressure and the number of pass increases, the polymer solution shows a significant decrease of viscosity and molecular weight.
• High Depolymerization Effect, Short Process Time
and No Waste Water (Green Chemistry)
- β-glucan structure
Cosmetic Food Drug
TextileDrug Delivery
System
Environment
 UV-Vis
 XRD
 Characterization
- Viscometer
- Gel Chromatograph
- FT-IR
- UV-Vis
- XRD
• The intensity characteristics of the
peaks tend to decrease with
increasing numbers of passes under
high pressure homogenization.
• A decrease in crystallinity occurs
due to the destruction of the crystal
structure of chitosan as a result of
the cleavage of the glycosyl bonds
200 250 300 350 400 450
Absorbance
Wavelength (nm)
5 Passes
3 Passes
1 Pass
0 Pass
1500 bar
5 10 15 20 25 30 35 40
Intensity(arb.unit)
2 Theta (degree)
0 Pass
1 Pass
3 Passes
5 Passes