The document summarizes a study on using biochemical processes to control and mitigate industrial wastewater from Reckitt Benckiser Bangladesh Limited. The study tested various treatment processes including coagulation-flocculation, Fenton process, and aerobic treatment. Through these processes, COD was reduced by nearly 99% and final effluent parameters met Department of Environment standards with COD of 36 mg/L and BOD of 25.7 mg/L. The multi-stage treatment system effectively treated wastewater and improved upon the limitations of the company's previous effluent treatment plant.

1. Biochemical Process as a means to
Control and Mitigate Industrial
Wastewater
Presented By:
Md. Dain Shah Munna
ID: 14209002
M.S.: 2017-2018
Department of Applied Chemistry and Chemical
Engineering,
University of Chittagong.

2. CONTENT
1 INTRODUCTION
2
STUDY AREA
3
AIMS AND OBJECTIVES
4
RESULTS AND DISCUSSION
CONCLUSION
5
6
7
RESEARCH MODEL
ACKNOWLEDGEMENTS
METHODOLOGY
8

3. INTRODUCTION
Plays a dynamic role in economic
growth as well as the
environmental sectors of
Bangladesh.
Industry
01 Industrial wastewater produced a
significant sources of pollution in the
environment.
Pollution
02
High amounts of Chemical Oxygen
Demand (COD) and Total
Dissolved Solids (TDS).
Complex Material
03 Using biochemical processes as
Coagulation-flocculation process,
Fenton process, Aerobic treatment
process.
Control and Mitigate
04

4. AIMS AND OBJECTIVES
 Determine the concentration of pH, COD, BOD, DO, TDS,
TSS/MLSS, MLVSS, Conductivity, Salinity, and Resistivity of
the wastewater.
 Control and Mitigate the wastewater by using both biological and
chemical treatment processes for an effluent treatment plant.
 Discharge water parameters should be meet within the
permissible limits of the Department of Environment (DoE),
Bangladesh.

5. STUDY AREA
Your Text Here
 Reckitt Benckiser (RB) Bangladesh Limited, East Nasirabad, Chattogram,
Bangladesh

6. Raw Materials:
 Hydrochloric acid, Caustic soda, Pine oil,
Dye-stuffs, Surfactants and many others
complex organic and inorganic compounds.
Reckitt Benckiser (RB) Bangladesh Limited
Products:
 Toilet cleaner – Harpic
 Floor cleaner – Lizol
 Glass cleaner – Mr. Brasso
 Dish washing liquid – Trix
 Dettol Antiseptic Liquid
 Dettol Liquid Hand Wash
 Dettol Soap

7. RB’s Previous Effluent Treatment Plant

8. Limitations of previous ETP:
 Equalization Tank effluent pH was very low (pH 1-2).
 Aerobic process was insufficient to meet high COD.
 One Step Chemical treatment was unable to reduce high COD.
 TDS was poorly met the DoE standard.
Key Points to solve the limitations:
 Adding different processes to improve this system.
 Treatment with the different coagulants to find the best reaction.
 Introduction of Fenton reagent for reducing COD value.
DoE=Department of Environment, Bangladesh

9. DoE STANDARD FOR WASTEWATER
Parameter Range
pH 6.0 - 9.0
DO 4.5 - 8.0 mg/L
COD < 200 mg/L
BOD < 50 mg/L
TDS < 2100 mg/L
Conductivity <1200 µS/cm
Resistivity -
Salinity -
TSS <500 mg/L
DoE=Department of Environment, Bangladesh

10. Research Model
Wastewater Inlets
Equalization
1st Chemical Treatment (Coagulation & Flocculation Process)
1st Biological Treatment (Activated sludge process)
2nd Chemical Treatment (Advanced Oxidation Process as Fenton process)
2nd Biological Treatment (Activated sludge process)
Filtered Water (Sand Filter & Carbon Filter)
Reverse Osmosis Process
Outlet

11. Equalization Tank
Equalization
Tank
Wastewater Inlet-1
(conc. HCl, H2SO4
and acidic organic and
inorganic compounds)
Wastewater Inlet-2
(Soap, Detergents raw
materials and Sodium
salts) Wastewater Inlet-3
(Laboratory testing
unit waste water)

12. 1st Chemical Treatment:
Coagulation & Flocculation Process
Lime Polyaluminum Chloride (PAC) Polymer HCl
5 gm/L 1 gm/L 10 mg/L If needed.

13. 2nd Chemical Treatment:
Advanced Oxidation Process as Fenton process
Fenton reagent (FeSO4 + H2O2) used to reduce the high COD level to expected
COD level.
Iron (II) is oxidized by hydrogen peroxide to Iron (III), forming a hydroxyl
radical and a hydroxide ion in the process. Iron (III) is then reduced back to
iron (II) by another molecule of hydrogen peroxide, forming a
hydroperoxyl radical and a proton.
Fe2+ + H2O2 → Fe3+ + HO• + OH− (1)
Fe3+ + H2O2 → Fe2+ + HOO• + H+ (2)
Hydroxyl is a powerful oxidant. Oxidation of an organic or inorganic
compound by Fenton's reagent is rapid and exothermic.

14. Advanced Oxidation Process as Fenton process:
COD Reduction for Sample Tests
Combination No. FeSO4 (gm) H2O2 (ml) COD Reduction percentage (%)
1 5 150 93
2 5 120 92
3 5 100 91
4 5 80 88
5 4 80 87
6 4 60 86
7 4 50 84
8 4 40 83
9 4 30 79
10 4 20 73
11 4 15 70
12 4 12 64
13 4 8 58
14 3 9 43
15 3 8 34
16 2 6 30
17 2 4 23

15. Biological Treatment: Activated Sludge Process
The biological treatment, aerobic treatment as the activated sludge process, wastewater
treatment is based on providing intimate contact between wastewater and biologically
active sludge.

16. Reverse Osmosis Process
Reverse osmosis (RO) can remove all the dissolved solids except water and its associated ions.
It can effectively lower dissolved solids and produce high-quality water.

17. METHODOLOGY
Parameters Unit Methods/Instruments
pH - Hach HQ40d Portable Multi-Parameter Meter
Chemical Oxygen Demand (COD) mg/L APHA 5220 B. Open Reflux Method
Biological Oxygen Demand (BOD) mg/L APHA 5210 B. 5-Day BOD Test
Dissolved Oxygen (DO) mg/L Hach HQ40d Portable Multi-Parameter Meter
Total Dissolved Solids (TDS) mg/L Hach HQ40d Portable Multi-Parameter Meter
Conductivity µS/cm Hach HQ40d Portable Multi-Parameter Meter
Resistivity Ω-cm Hach HQ40d Portable Multi-Parameter Meter
Salinity % Hach HQ40d Portable Multi-Parameter Meter
Total Suspended Solids (TSS) mg/L APHA 2540 D. Total Suspended Solids
Mixed Liquor Suspended Solids (MLSS) mg/L APHA 2540 D. Total Suspended Solids
Mixed Liquor Volatile Suspended Solids
(MLVSS)
mg/L APHA 2540 E. Volatile Solids
* APHA = American Public Health Association

18. RESULTS AND DISCUSSION
Effluent generation
Parameter Wastewater Inlet-1 Wastewater Inlet-2 Wastewater Inlet-3 Equalization Tank
pH 0.86 7.94 3.13 2.12
DO 6.47 mg/L 7.02 mg/L 6.55 mg/L 8.12 mg/L
COD 60,000 mg/L 468 mg/L 965 mg/L 22000 mg/L
BOD 100 mg/L 76 mg/L 138 mg/L 112 mg/L
TDS 45400 mg/L 847 mg/L 1075 mg/L 4600 mg/L
Conductivity 75400 µS/cm 1691 µS/cm 2128 µS/cm 8600 µS/cm
Resistivity 12.2 Ω-cm 592 Ω-cm 470 Ω-cm 116.4 Ω-cm
Salinity 46.10 % 0.85 % 1.09 % 4.80 %

19. RESULTS AND DISCUSSION
Effluent Treatment
Parameters After Coagulation &
Flocculation
Process
1st Biological Tr
eatment
After 2nd Chemical T
reatment
After 2nd Aerobic
Treatment
Filtered
Water
pH 7.2 7.4 7.5 7.55 7.11
DO 4.52 mg/L 3.29 mg/L 6.8 mg/L 4.23 mg/L 5.02 mg/L
COD 5123 mg/L 2736 mg/L 1193 mg/L 551 mg/L 50 mg/L
BOD 12 mg/L 175 mg/L 7 mg/L 72.4 mg/L 135 mg/L
TDS 3827 mg/L 3340 mg/L 3100 mg/L 3000 mg/L 2900 mg/L
Conductivity 7470 µS/cm 6280 µS/cm 5580 µS/cm 5030 µS/cm 4650 µS/cm
Resistivity 335 Ω-cm 159.2 Ω-cm 161.9 Ω-cm 167.1 Ω-cm 175.6 Ω-cm
Salinity 1.55 % 3.42 % 3.10 % 2.92 % 2.78 %
TSS 2034 mg/L - 228 mg/L - 24 mg/L
MLSS - 2552 mg/L - 2070 mg/L -
MLVSS - 2016 mg/L - 1718 mg/L -

20. RESULTS AND DISCUSSION
Final Effluent with DoE Standard
Parameter Outlet DoE Standard Range
pH 6.95 6.0 - 9.0
DO 6.34 mg/L 4.5 - 8.0 mg/L
COD 36 mg/L < 200 mg/L
BOD 25.7 mg/L < 50 mg/L
TDS 61.8 mg/L < 2100 mg/L
Conductivity 130.7 µS/cm <1200 µS/cm
Resistivity 7650 Ω-cm -
Salinity 0.06 % -
TSS 10 mg/L <500 mg/L

21. Evaluations of pH
2.12
7.2 7.4 7.5 7.55
7.11
6.95
0
1
2
3
4
5
6
7
8
Equalization Coagulation
&
Flocculation
1st Aerobic
Treatment
Fenton
Process
2nd Aerobic
Treatment
Filtered
Water
After RO
Process
(Outlet)
pH
Steps of treatment
pH

22. Evaluations of DO
8.12
4.52
3.29
6.8
4.23
5.02
6.34
0
1
2
3
4
5
6
7
8
9
Equalization Coagulation
&
Flocculation
1st Aerobic
Treatment
Fenton
Process
2nd Aerobic
Treatment
Filtered
Water
After RO
Process
(Outlet)
mg/L
Steps of treatment
DO (mg/L)

23. Evaluations of COD
22000
5123
2736 1193 551 50 36
0
5000
10000
15000
20000
25000
Equalization Coagulation
&
Flocculation
1st Aerobic
Treatment
Fenton
Process
2nd Aerobic
Treatment
Filtered
Water
After RO
Process
(Outlet)
mg/L
Steps of treatment
COD (mg/L)

24. Evaluations of BOD
112
12
175
7
72.4
135
25.7
0
20
40
60
80
100
120
140
160
180
200
Equalization Coagulation
&
Flocculation
1st Aerobic
Treatment
Fenton
Process
2nd Aerobic
Treatment
Filtered
Water
After RO
Process
(Outlet)
mg/L
Steps of treatment
BOD (mg/L)

25. Evaluations of TDS
4600
3827
3340
3100 3000
2900
61.8
0
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
Equalization Coagulation
&
Flocculation
1st Aerobic
Treatment
Fenton
Process
2nd Aerobic
Treatment
Filtered
Water
After RO
Process
(Outlet)
mg/L
Steps of treatment
TDS

26. Evaluations of Conductivity
8600
7470
6280 5580
5030
4650
130.7
0
1000
2000
3000
4000
5000
6000
7000
8000
9000
10000
Equalization Coagulation
&
Flocculation
1st Aerobic
Treatment
Fenton
Process
2nd Aerobic
Treatment
Filtered
Water
After RO
Process
(Outlet)
µS/cm
Steps of treatment
Conductivity

27. Evaluations of Resistivity
116.4 134 159.2 161.9 167.1 175.6
7650
0
1000
2000
3000
4000
5000
6000
7000
8000
9000
Equalization Coagulation
&
Flocculation
1st Aerobic
Treatment
Fenton
Process
2nd Aerobic
Treatment
Filtered
Water
After RO
Process
(Outlet)
Ω-cm
Steps of treatment
Resistivity

28. Evaluations of Salinity
4.80%
4.00%
3.42%
3.10% 2.92%
2.78%
0.06%
0.00%
1.00%
2.00%
3.00%
4.00%
5.00%
6.00%
Equalization Coagulation
&
Flocculation
1st Aerobic
Treatment
Fenton
Process
2nd Aerobic
Treatment
Filtered
Water
After RO
Process
(Outlet)
Percentage
Steps of treatment
Salinity

29. Evaluations of TSS
2292 2034
228
24 10
0
500
1000
1500
2000
2500
Equalization Coagulation &
Flocculation
Fenton Process Filtered Water After RO Process
(Outlet)
mg/L
Steps of treatment
TSS

30. Evaluations of MLSS & MLVSS
0
500
1000
1500
2000
2500
3000
1st Aerobic Treatment 2nd Aerobic Treatment
2552
2070
2016
1718
mg/L
Aerobic treatment process
MLSS & MLVSS
MLSS (mg/L) MLVSS (mg/L)

31. New Effluent Treatment Plant

32. CONCLUSION
 In Coagulation & Flocculation process, COD reduces almost 76% from equalization.
 In 1st Aerobic treatment, COD reduce 46% from Coagulation and Flocculation.
 In the Fenton process, it reduces 56% from 1st Aerobic treatment.
 In 2nd Aerobic treatment, COD reduce 54% from the Fenton process.
 After 2nd Aerobic treatment, COD reduce almost 90%.
 Ultimately COD removal efficiency is almost 99% from equalization through all chemical and
biological treatment.
In the Final Steps:
 The COD value is 36 mg/L, (DoE permissible limit < 200 mg/L).
 The BOD value is 25.7 mg/L, (DoE permissible limit <50 mg/L).
 The DO value is 6.34 mg/L, (DoE permissible limit 4.5-8) mg/L.
 TDS value is 61.8 mg/L, (DoE permissible limit <2100 mg/L.)
 MLSS value is greater than 2000 mg/L, indicates the effective aerobic process.
 The value for MLVSS/MLSS ratio 0.79 to 0.83 was achieved where the MLVSS/MLSS ratio is
often believed to be about 0.75.

33. ACKNOWLEDGEMENT
 Firstly, I would like to extend my whole-hearted thanks and appreciations to my supervisor,
Dr. Md. Didarul Alam Chowdhury, Assistant Professor, Department of Applied Chemistry
and Chemical Engineering, University of Chittagong, for his enormous support and
guidance during supervision of this research. His encouragement, intensive mentoring and
invaluable feedback have enabled me to produce this thesis.
 I also want thanks to Founder Chairman and Professor Dr. Mohammad Helal Uddin, for
his pleasantness and moral support throughout my research journey.
 It is a pleasure to thanks to the authorities of Reckitt Benckiser Bangladesh Limited in
Chattogram to provide experiment facility and supporting me on this thesis project.
 Sincerity gratitude to Mr. Sagar Barua at KDS Textile Mills Ltd. for his great support and
help on the materials and theoretical guidance.

34. Thank You