This document outlines statistical tests to select based on experimental design factors. It recommends using a t-test or Z-test for unmatched or matched data with one variable comparing two samples, one-way ANOVA and Tukey's test for comparing two or more samples with one variable, two-way ANOVA and Tukey's test when having two variables with all categories combined, two-way nested ANOVA when one variable is nested within another, and three-way ANOVA to test hypotheses when having three treatment variables with all categories combined.