In recent years, it is no doubt that in India, where on one side pollution is increasing day by day due to accumulation of organic waste and on the other side there is a great shortage of organic manure.
It has been estimated that India, as a whole, generates as much as 25 million tonnes of urban solid waste of diverse composition per year. Solid waste comprises of both organic and inorganic matter.
Under the present condition of environmental degradation, vermicomposting technology is the best way to meet all the requirements of the society. This is a process of recycling trash/agricultural wastes in an efficient and eco-friendly manner in order to produce quality compost.
Organic wastes can be broken down and fragmented rapidly by earthworms, resulting in a stable non-toxic material with good structure, which has a potentially high economic value and also acts as a soil conditioner for plant growth.
It is a type of composting in which worms eat and metabolize organic matter that comprises to a better end product known as Vermicast (commonly called as BLACK GOLD) which has a stuff of nutrients that can be directly incorporated into the soil to help with plant fertilization, soil enrichment and soil stability.From a social point of view, organic fertilizers will:
Improve the social status of the individuals and the community.
Create motivation for people to live in the countryside by providing job
opportunities and business plans.
From a hygienic point of view, organic fertilizers will:
Produce chemical-free crops which will improve people's health.
Reduce the danger of lung diseases and other diseases resulting from burning the organic wastes in the field.EPIGEIC EARTHWORMS:
Earthworms of this group cannot make burrows in the soil. They can only move through crevices of the surface. They feed exclusively on decomposing organic wastes.
ENDOGEIC EARTHWORMS:
They are subsoil dwellers. Secretions of body wall of earthworms cement and smoothen the walls of the burrows and protect the wall from collapsing easily. They move below 30cm or more in the soil
ANECIC EARTHWORMS:
They are found in the soil, which is not frequently disturbed. They make very complicated burrows in the sol and they firmly pack their burrow walls with their castings. The Anecic earthworms like Epigeic earthworms are commonly found in temperate countries.Vermicompost is an excellent soil additive made up of digested compost. Worm castings are much higher in nutrients and microbial life and therefore, are considered as a higher value product. Worm castings contain up to 5 times the plant available nutrients. It not only adds microbial organisms and nutrients that have long lasting residual effects, it also modulates structure to the existing soil, increases water retention capacity. Vermicompost contains an average of 1.5% - 2.2% N, 1.8% - 2.2% P and 1.0% - 1.5% K. The organic carbon is ranging from 9.15 to 17.98 and contains micronutrients Nitrogen, phosphorus, Potassium..
In recent years, it is no doubt that in India, where on one side pollution is increasing day by day due to accumulation of organic waste and on the other side there is a great shortage of organic manure.
It has been estimated that India, as a whole, generates as much as 25 million tonnes of urban solid waste of diverse composition per year. Solid waste comprises of both organic and inorganic matter.
Under the present condition of environmental degradation, vermicomposting technology is the best way to meet all the requirements of the society. This is a process of recycling trash/agricultural wastes in an efficient and eco-friendly manner in order to produce quality compost.
Organic wastes can be broken down and fragmented rapidly by earthworms, resulting in a stable non-toxic material with good structure, which has a potentially high economic value and also acts as a soil conditioner for plant growth.
It is a type of composting in which worms eat and metabolize organic matter that comprises to a better end product known as Vermicast (commonly called as BLACK GOLD) which has a stuff of nutrients that can be directly incorporated into the soil to help with plant fertilization, soil enrichment and soil stability.From a social point of view, organic fertilizers will:
Improve the social status of the individuals and the community.
Create motivation for people to live in the countryside by providing job
opportunities and business plans.
From a hygienic point of view, organic fertilizers will:
Produce chemical-free crops which will improve people's health.
Reduce the danger of lung diseases and other diseases resulting from burning the organic wastes in the field.EPIGEIC EARTHWORMS:
Earthworms of this group cannot make burrows in the soil. They can only move through crevices of the surface. They feed exclusively on decomposing organic wastes.
ENDOGEIC EARTHWORMS:
They are subsoil dwellers. Secretions of body wall of earthworms cement and smoothen the walls of the burrows and protect the wall from collapsing easily. They move below 30cm or more in the soil
ANECIC EARTHWORMS:
They are found in the soil, which is not frequently disturbed. They make very complicated burrows in the sol and they firmly pack their burrow walls with their castings. The Anecic earthworms like Epigeic earthworms are commonly found in temperate countries.Vermicompost is an excellent soil additive made up of digested compost. Worm castings are much higher in nutrients and microbial life and therefore, are considered as a higher value product. Worm castings contain up to 5 times the plant available nutrients. It not only adds microbial organisms and nutrients that have long lasting residual effects, it also modulates structure to the existing soil, increases water retention capacity. Vermicompost contains an average of 1.5% - 2.2% N, 1.8% - 2.2% P and 1.0% - 1.5% K. The organic carbon is ranging from 9.15 to 17.98 and contains micronutrients Nitrogen, phosphorus, Potassium..
ORGANIC FARMING : COMMON ORGANIC MANURES SMGsajigeorge64
A brief account of common organic manures - Bone meal, cow dung, poultry wastes, oil cakes, organic mixtures, compost and vermicompost, vermiwash , advantages and disadvantages of composting & vermicomposting.
Composting is nature's process of recycling decomposed organic materials into a rich soil known as compost. Anything that was once living will decompose
ORGANIC FARMING : COMMON ORGANIC MANURES SMGsajigeorge64
A brief account of common organic manures - Bone meal, cow dung, poultry wastes, oil cakes, organic mixtures, compost and vermicompost, vermiwash , advantages and disadvantages of composting & vermicomposting.
Composting is nature's process of recycling decomposed organic materials into a rich soil known as compost. Anything that was once living will decompose
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Gliese 12 b: A Temperate Earth-sized Planet at 12 pc Ideal for Atmospheric Tr...Sérgio Sacani
Recent discoveries of Earth-sized planets transiting nearby M dwarfs have made it possible to characterize the
atmospheres of terrestrial planets via follow-up spectroscopic observations. However, the number of such planets
receiving low insolation is still small, limiting our ability to understand the diversity of the atmospheric
composition and climates of temperate terrestrial planets. We report the discovery of an Earth-sized planet
transiting the nearby (12 pc) inactive M3.0 dwarf Gliese 12 (TOI-6251) with an orbital period (Porb) of 12.76 days.
The planet, Gliese 12 b, was initially identified as a candidate with an ambiguous Porb from TESS data. We
confirmed the transit signal and Porb using ground-based photometry with MuSCAT2 and MuSCAT3, and
validated the planetary nature of the signal using high-resolution images from Gemini/NIRI and Keck/NIRC2 as
well as radial velocity (RV) measurements from the InfraRed Doppler instrument on the Subaru 8.2 m telescope
and from CARMENES on the CAHA 3.5 m telescope. X-ray observations with XMM-Newton showed the host
star is inactive, with an X-ray-to-bolometric luminosity ratio of log 5.7 L L X bol » - . Joint analysis of the light
curves and RV measurements revealed that Gliese 12 b has a radius of 0.96 ± 0.05 R⊕,a3σ mass upper limit of
3.9 M⊕, and an equilibrium temperature of 315 ± 6 K assuming zero albedo. The transmission spectroscopy metric
(TSM) value of Gliese 12 b is close to the TSM values of the TRAPPIST-1 planets, adding Gliese 12 b to the small
list of potentially terrestrial, temperate planets amenable to atmospheric characterization with JWST.
2. COMPOSTING
• Composting is natural way of recycling.
• Composting is the microbial process of degradation in which the
plant and animal wastes are naturally degraded to produce organic
manure. Organic manure is used for enrichment of the soil that helps
with the growth of plants.
• Microorganisms feed on the materials added to the compost pile
during the composting process. They use carbon and nitrogen to
grow and reproduce, water to digest materials, and oxygen to
breathe.
2
4. INGREDIENTS FOR COMPOSTING
The ingredients for composting include a proper balance of the following
materials:
• Carbon-rich materials (“browns”) can include dry leaves, plant stalks, and
twigs. The carbon-rich materials provide food for the microorganisms to
consume and digest.
• Nitrogen-rich materials (“greens”) include grass clippings and food scraps.
The nitrogen-rich materials heat up the pile to create ideal conditions for the
material to breakdown.
• Water (moisture).
• Air (oxygen).
4
5. • Methods of composting
Methods of
composting
Aerobic method
Anaerobic method
5
6. 1. Aerobic method
• Aerobic composting is decomposition of organic matter using
microorganisms that require oxygen.
• The microbes responsible for composting are naturally occurring
and live in the moisture surrounding organic matter.
• Oxygen from the air diffuses in to the moisture and is taken up by
the microbes. As aerobic digestion takes place the by-products are
heat, water and carbon dioxide (CO2).
• While CO2 can be classified as a greenhouse gas it’s evolution
from the composting process is not counted in emissions.
Additionally, CO2 is only 1/20th as harmful to the environment as
methane (the main by-product of anaerobic degradation).
6
7. • The heat produced in aerobic composting is sufficient to kill
harmful bacteria and pathogens as these organisms are not
adapted to these environmental conditions.
• It also helps support the growth of beneficial bacteria species
including psychrophilic, mesophilic, and thermophilic bacteria
which thrive at the higher temperature levels.
• From start to finish, the HotRot in-vessel aerobic composting
process takes only 8-10 days.
• No leachate is produced as any surplus moisture is extracted as
water vapor which can be condensed and used for watering nearby
vegetation.
7
9. 2. Anaerobic method
• Anaerobic composting is decomposition that occurs using microorganisms
that do not require oxygen to survive.
• In an anaerobic system the majority of the chemical energy contained
within the starting material is released as methane.
• The process is characterised by very strong odours and only a small
amount of heat is generated meaning decomposition takes much longer
and doesn’t reach sufficient temperatures to safely kill plant pathogens,
weed and seeds. To overcome these limitations external (artificial) heat is
normally added.
• As the material is broken down by anaerobic digestion, it creates a sludge-
like material that is even more difficult to break down.
• This material, digestate, typically requires aerobic composting to complete
the stabilisation process.
9
13. VERMICOMPOSTING
• Worm composting, or vermicomposting, is another method of
composting by using earthworms.
• It takes up little space, the materials are simple and
inexpensive, and it can be done indoors or outdoors.
• It need a container or bin, bedding material, worms, and food
scraps.
• If worm bin is properly maintained, it should not emit odors or
attract pests. The resulting product is vermicompost, a soil
amendment.
13
14. • Vermicomposting comprises two methods:
1. Bed Method: This is an easy method in which beds of organic
matter are prepared.
2. Pit Method: In this method, the organic matter is collected in
cemented pits. However, this method is not prominent as it involves
problems of poor aeration and waterlogging.
14
16. PROCEDURE OF VERMICOMPOSTING
• To prepare compost, either a plastic or a concrete tank can be used.
The size of the tank depends upon the availability of raw materials.
• Collect the biomass and place it under the sun for about 8-12 days.
Now chop it to the required size using the cutter.
• Prepare a cow dung slurry and sprinkle it on the heap for quick
decomposition.
• Add a layer (2 – 3 inch) of soil or sand at the bottom of the tank.
• Now prepare fine bedding by adding partially decomposed cow dung,
dried leaves and other biodegradable wastes collected from fields and
kitchen. Distribute them evenly on the sand layer.
16
17. • Continue adding both the chopped bio-waste and partially
decomposed cow dung layer-wise into the tank up to a depth of
0.5-1.0 ft.
• After adding all the bio-wastes, release the earthworm species over
the mixture and cover the compost mixture with dry straw or gunny
bags.
• Sprinkle water on a regular basis to maintain the moisture content of
the compost.
• Cover the tank with a thatch roof to prevent the entry of ants, lizards,
mouse, snakes, etc. and protect the compost from rainwater and
direct sunshine.
• Have a frequent check to avoid the compost from overheating.
Maintain proper moisture and temperature.
17
20. Advantages Of Vermicomposting
The major benefits of vermicomposting are:
• Develops roots of the plants.
• Improves the physical structure of the soil.
• Vermicomposting increases the fertility and water-resistance of
the soil.
• Helps in germination, plant growth, and crop yield.
• Nurtures soil with plant growth hormones such as auxins,
gibberellic acid, etc.
20
21. Disadvantages of Vermicomposting
Following are the important disadvantages of vermicomposting:
• It is a time-consuming process and takes as long as six months to
convert the organic matter into usable forms.
• It releases a very foul odour.
• Vermicomposting is high maintenance. The feed has to be added
periodically and care should be taken that the worms are not flooded
with too much to eat.
• The bin should not be too dry or too wet. The moisture levels need to
be monitored periodically.
• They nurture the growth of pests and pathogens such as fruit flies,
centipede and flies.
21