1. Humoral immunity is mediated by antibodies that are secreted and perform effector functions at distant sites from their production. Antibodies neutralize microbes and microbial toxins.
2. The complement system assists antibodies in lysing bacteria. It involves a cascade of proteins that are activated sequentially and amplify the immune response. Activation can occur via the classical, lectin, or alternative pathways.
3. The classical pathway is initiated by the binding of the C1 complex to antibodies bound to pathogens. This activates a protease cascade leading to formation of the membrane attack complex that lyses microbes. The lectin pathway uses mannose-binding lectin and ficolins instead of antibodies. The alternative pathway is antibody
Describes the complement system components and their activation pathways, the regulation of the complement
system, the effector functions of various complement components,
and the consequences of deficiencies in them.
Describes the complement system components and their activation pathways, the regulation of the complement
system, the effector functions of various complement components,
and the consequences of deficiencies in them.
The complement system is a part of the immune system that helps or complements the ability of antibodies and phagocytic cells to clear pathogens from an organism. It is part of the innate immune system, which is not adaptable and does not change over the course of an individual's lifetime.
consists of three pathways: 1. alternative
2. classical
3. lectin pathway
Type II Hypersensitivity-Antibody mediated cytotoxic HypersensitivityAnup Bajracharya
Type II Hypersensitivity is antibody-mediated immune reaction in which antibodies (IgG or IgM) are directed against cellular or extracellular matrix antigens with the resultant cellular destruction, functional loss, or damage to tissues.
The term ‘complement’ refers to set of serum proteins that cooperate in both innate and adaptive immune system to eliminate blood tissue pathogens.
It was 1st identified as heat labile component of serum.
Major effectors of hormonal branch of immune system.
Paul Ehrlich in Berlin independently carried out similar experiments & coined the term COMPLEMENT, defining it as ‘’ the activity of blood serum that completes the action of antibody.’’
In later years it was revealed that the action of complement is basically the result of interaction of large & complex group of proteins.Most of the components of complement system are synthesized in liver by hepatocytes, epithelial cells of gastrointestinal & genitourinary tracts.
it consist up of 15% of globular proteins fraction in plasma & combined conc. Is about 3 mg/ml.
These are the glycoproteins distributed among blood plasma & cell membrane.After activation several components interact in regulated cascade to carry out no. of basic functions…..
Lysis if cells .( bacteria , virus)
Opsonization, that promote phagocytosis of particulate antigens.
Activation of inflammatory response.
Immune clearance.
Chemotaxis.
Lysis refers to the breaking down of the cells' membrane , by viral, enzymic, or osmotic mechanisms that compromise its integrity.
A fluid containing the contents of lysed cells is called a "lysate".
Cell lysis is used to break open cells to avoid shear forces that would denature or degrade sensitive proteins and DNA.
Complement System comprises of Complement proteins that function to augment the antibodies in killing bacteria by the formation of Membrane Attack Complex.
This ppt describes the different pathways of activation complement proteins and MAC formation.
The complement system is a part of the immune system that helps or complements the ability of antibodies and phagocytic cells to clear pathogens from an organism. It is part of the innate immune system, which is not adaptable and does not change over the course of an individual's lifetime.
consists of three pathways: 1. alternative
2. classical
3. lectin pathway
Type II Hypersensitivity-Antibody mediated cytotoxic HypersensitivityAnup Bajracharya
Type II Hypersensitivity is antibody-mediated immune reaction in which antibodies (IgG or IgM) are directed against cellular or extracellular matrix antigens with the resultant cellular destruction, functional loss, or damage to tissues.
The term ‘complement’ refers to set of serum proteins that cooperate in both innate and adaptive immune system to eliminate blood tissue pathogens.
It was 1st identified as heat labile component of serum.
Major effectors of hormonal branch of immune system.
Paul Ehrlich in Berlin independently carried out similar experiments & coined the term COMPLEMENT, defining it as ‘’ the activity of blood serum that completes the action of antibody.’’
In later years it was revealed that the action of complement is basically the result of interaction of large & complex group of proteins.Most of the components of complement system are synthesized in liver by hepatocytes, epithelial cells of gastrointestinal & genitourinary tracts.
it consist up of 15% of globular proteins fraction in plasma & combined conc. Is about 3 mg/ml.
These are the glycoproteins distributed among blood plasma & cell membrane.After activation several components interact in regulated cascade to carry out no. of basic functions…..
Lysis if cells .( bacteria , virus)
Opsonization, that promote phagocytosis of particulate antigens.
Activation of inflammatory response.
Immune clearance.
Chemotaxis.
Lysis refers to the breaking down of the cells' membrane , by viral, enzymic, or osmotic mechanisms that compromise its integrity.
A fluid containing the contents of lysed cells is called a "lysate".
Cell lysis is used to break open cells to avoid shear forces that would denature or degrade sensitive proteins and DNA.
Complement System comprises of Complement proteins that function to augment the antibodies in killing bacteria by the formation of Membrane Attack Complex.
This ppt describes the different pathways of activation complement proteins and MAC formation.
Through this presentation you will be able to learn about the detailed knowledge of complement system and its functions along with the complement activation pathways [classical, alternative, lectin pathway ]
This presentation describes the Fish Complement system and different types of pathways involved and the mechanism behind the regulation of complement proteins. It gives a basic and a detailed explanation regarding the topic.
Immunology is the study of the immune system and is a very important branch of the medical and biological sciences. The immune system protects us from infection through
Factors affecting each of the proceses- Absorption, Distribution, Metabolism and Elimination of Drugs and associated Pharmacokinetic Parameters- Bioavailability, Volume of Distribution, Half life of drug, Rate of Clearance
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
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In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
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under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
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heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
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2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
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Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
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This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
1. Akash Mahadev Iyer
S3-M.Sc Biochemistry
Department of biochemistry
University of Kerala
Karyavattom campus
2. Humoral immunity is mediated by secreted antibodies
The main function of antibodies is the neutralization and elimination
of infectious microbes and microbial toxins.
Humoral immunity is transferrable from immunized to naïve
individuals.
Antibodies -produced by plasma cells in secondary lymphoid organs
and bone marrow
Perform their effector functions at sites distant from their production.
Many of the effector functions of antibodies are mediated by the heavy
chain constant regions of Ig molecules.
3.
4. Complement system
The name “COMPLEMENT” is derived from experiments performed by
Jules Bordet.
He demonstrated that;
Fresh serum containing antibacterial antibody
Added to bacterial culture at Physiological temperature(37 oC)
Bacteria are lysed
If the serum was heated to 56o C or more ,the serum loses it’s lytic
capacity
Bordet concluded the presence of heat labile component
that assists or complements , the lytic function of antibodies-Hence
the name ‘Complement’
5. The complement system consists of serum and cell surface proteins
Interact with one another and with other molecules of immune system
in a highly regulated manner to generate products that function to
eliminate microbes.
6. Features of complement activation
The complements system is inactive in healthy, uninfected animals.
It is activated by either PAMPs on the surface of infectious agents or by
antigen bound antibodies
The complement system is activated by microbes and by antibodies that
are attached to microbes and other antigens
Activation of complement involves the sequential proteolysis of proteins
to generate enzyme complexes with proteolytic activity- Sequential
zymogen activation.
The products of complement activation become covalently attached to
microbial cell surfaces, to antibodies bound to microbes and to other
antigens, and to apoptotic bodies
Regulatory proteins minimize complement mediated damage to host cells
7. Complement proteins
Synthesized mainly by liver hepatocytes, (blood monocytes, tissue macrophages, and
epithelial cells of the gastrointestinal and genitourinary tracts.)
These components constitute 5% (by weight) of the serum globulin fraction.
Numeral designation (C1–C9), by letter symbols (e.g., factor D), or by trivial names
(e.g., homologous restriction factor).
Peptide fragments formed by activation of a component - the larger fragments: bind to
the target near the site of activation - the smaller fragments: local inflammation
Complexes with enzymatic activity are designated by a bar over the number or
symbol.
Most circulate in the serum in functionally inactive forms as zymogens.
Smaller fragment
Larger fragment
C2 is an exception: C2a
is the larger cleavage
fragment).
C4
C4a
C4b
8.
9.
10. Pathway begins with formation of soluble Ag-Ab complexes (immune
complexes) or with binding of antibody to antigen on a suitable target, such as
a bacterial cell.
Activated by IgM and certain subclasses of IgG (human IgG1, IgG2, and IgG3).
The initial stage of activation involves C1, C2, C3, and C4, which are present in
plasma in functionally inactive forms.
11. Macromolecular complex consists of
C1q and
Two molecules each of C1r and C1s, held together in a complex (C1qr2s2) stabilized by
Ca2+ ions.
C1q- composed of 18 polypeptide chains that associate to form six collagen-like triple
helical arms, the tips of which bind to exposed C1q-binding sites in the CH2 domain of
the antibody molecule.
Each C1r and C1s monomer contains
1. Catalytic domain and
2. Interaction domain; (facilitates interaction with C1q or with each other.)
12. The formation of Ag-Ab complex induces a conformational changes
in the Fc portion of the IgM molecule that exposes a binding site for
the C1 component of the complement system.
13. Binding of C1q to Fc binding sites induces conformational change in
C1r that auto-catalytically activates C1r to an active serine protease
enzyme, C1r, which then cleaves C1s to active enzyme, C1s.
C1q can also bind to apoptotic and necrotic cells, ECM proteins, CRP,
amyloid and prion proteins
¯ ¯
14. C1s has two substrates,
• C4 and
• C2.
C1s activates C4 by hydrolysis of
small fragment (C4a), exposing a
binding site on the larger fragment
(C4b).
The C4b fragment attaches to target
surface near C1, and the C2
proenzyme then attaches to the
exposed binding site on C4b, where C2
is then cleaved by neighboring C1s;
the smaller fragment (C2b) diffuses
away. (C1s cannot act on soluble C2).
The resulting C4b2a complex (potent
protease) is called C3 convertase,
(converts C3 into its active form.)
¯
¯
15. Binding to C3 is mediated by C4b component and
the proteolysis is catalysed by C2a component
The native C3 component consists of
two polypeptide chains, α and β.
Hydrolysis of a short fragment (C3a)
from the amino terminus of the chain by
the C3 convertase generates C3b.
(A single C3 convertase molecule can
generate over 200 molecules of C3b,
resulting in tremendous amplification).
Some of the C3b binds to C4b2a to form
a trimolecular complex C4b2a3b, called
C5 convertase.
16. The C3b component of this
complex binds C5 and alters its
conformation, so that the C4b2a
component can cleave C5 into
C5a, which diffuses away, and
C5b, attaches to C6 and
initiates formation of the
membrane attack complex
(MAC).
Some of the C3b generated by C3 convertase activity does not
associate with C4b2a; instead it diffuses away and then coats immune
complexes and particulate antigens, functioning as an opsonin.
17. • Activated by carbohydrates binding to a cell surface lectin, occurs in
pneumococcal infections.
• Splenic marginal zone macrophages express a cell surface C-type
lectin called SIGN-R1 that can recognize pneumococccal
polysaccharide and can also bind C1q.
• Binding of either bacteria or the polysaccharide (eg; 2-acetamido-4-
amino-2,4,6-trideoxy-d-galactose) to SIGN-R1 activates classical
pathway but without requirement for antibody.
• It can also be activated by cell surface bound pentraxins such as
CRP.
18. Lectins -proteins that recognize and bind to specific carbohydrate
targets.
The lectin pathway does not depend on antibody for its activation.
The pathway is activated by binding of MBL to mannose residues on
glycoproteins or carbohydrates on the surface of microorganisms
(Salmonella, Listeria, and Neisseria strains, as well as Cryptococcus
neoformans and Candida albicans.)
Microorganisms, bear on their surface repeating patterns of
molecular structures, known as pathogen-associated molecular
patterns (PAMPs).
20. Lipopolysaccharide of the outer membrane of Gram-negative
bacteria are not present on animal cells -important in the
recognition of bacteria by the innate immune system.
21. Glycans of yeast surface proteins terminate in
Glycans of vertebrate cells terminate in sialic acid residues (N-
acetylneuraminic acid) –MBL does not bind to mammalian
glycoproteins
The lectin pathway uses these features of microbial surfaces to detect
and respond to pathogens.
22. Complement activating lectins- MBL
and a family of proteins called ficolins.
MBL is an acute phase protein
produced in inflammatory responses.
MBL is a member of the collectin family
and has an N-terminal collagen like
domain and a C-terminal carbohydrate
recognition (lectin) domain
Ficolins (Fi+Col+Lin) -group of lectins
with subunits consisting of both
collagen (Col)-like long thin stretches
and fibrinogen (Fi)-like globular
domains with lectin (Lin) activity usually
specific for N-acetylglucosamine
(GlcNAc)
23.
24.
25. MBL and ficolins associate with MBL-associated serine proteases
(MASPs)-MASP1,MASP2 and MASP3.
MASP proteins are structurally homologus to the C1r and C1s
proteases.
Function- cleavage of C4 and C2- Activate the complement pathway
26. Once bound, MBL activates MBL-associated serine protease, MASP-2.
Activated MASP-2 acts on C4, splitting it into C4a and C4b.-This
exposes the thioester group on the C4b that generates a reactive
carbonyl group that covalently attaches C4b to microbial surface
Then C2 binds to C4b to form a complex C4b2
The bound C2 is cleaved by MASP-2 to generate C4b2b
Cell-bound C4b2b (protease) splits C3 to generate C3a and C3b
(MAJOR STEP)!!!!
Thioester group on C3b is exposed.
Each C4b2b complex can generate~ 200 C3b molecules
Newly formed C3b will bind to nearby microbes
Bound C3b binds to C5 and cleaves it to C5a and C5b
Complement pathway proceeds till completion.
27.
28. Individuals deficient in MBL or MASP-2 experience more respiratory
infections by common extracellular bacteria during early childhood,
Mutations in the gene can lead to MBL deficiency.
Incidence; 5–30 people in every 100.
Sometimes those affected may also contract more serious
infections, such as pneumonia and meningitis
29. Alternative Pathway
No antibody required- component of the innate immune system.
This major pathway of complement activation involves four serum proteins: C3, Factor
B, Factor D, and Properdin.
The alternative pathway is initiated by cell-surface constituents - foreign to the host
for example,- bacterial cell-wall constituents can activate the alternative pathway.
C3b component binds to foreign surface antigens (bacterial cells or viral particles or
even to the host’s own cells)
Membranes of most mammalian cells have high levels of sialic acid, -rapid inactivation
of bound c3b molecules on host cells;
Foreign antigenic surfaces (e.g., Bacterial cell walls, yeast cell walls, and certain viral
envelopes) have only low levels of sialic acid, c3b bound to these surfaces remains
active for a longer time.
In the classical pathway, C3 is rapidly
cleaved to C3a and C3b by enzymatic
activity of the C3 convertase
In the alternative pathway, serum C3, which
contains an unstable thioester bond, is
subject to slow spontaneous hydrolysis to
yield C3a and C3b
30. Synthesized by liver cells and
macrophages
Complement component of
highest concentration in serum
C3 is a disulfide linked hetero-
dimer with α and β chains.
Molecular weights 115K and 75K
(K = 103 Mr) respectively
An internal thioester is located in
the a chain between the cysteine
and glutamine residues in a
sequence Cys-Gly-Glu-Gln.
Upon activation, a fragment of 77
amino acids, C3a, is removed
from the N terminus of the
αchain.
The cleavage induces a
conformational change, C3b,
resulting in the exposure of the
thioester, (which is extremely
reactive with hydroxyl
nucleophiles)
31. The internal thioester from the four residues of –Cys–Gly–Glu–Gln–.
The two steps of the autolytic cleavage reaction are :
a. The formation of the internal pyroglutamic acid (1) and
b. The hydrolysis of the preceding peptide bond (2).
A thioester between the thiol of the cysteine residue and the acyl group of the
second glutamyl residue, forming a
32. C3 protein contains a reactive
thioester bond -buried in large
domain known as thioester
domain.
C3b covalently attaches to the
surface of cells, microbes, via the
thioester bond that reacts with the
amino or hydroxyl groups of cell
surface proteins or
polysaccharides to form amide or
ester bonds.
If these bonds are not formed,
the C3b remains in the fluid
phase, and the exposed and
reactive thioester bond is quickly
hydrolyzed, rendering the protein
inactive- complement activation
cannot proceed.
33. Then C3b undergoes post cleavage conformational change, a binding site
for a plasma protein called factor B is also exposed.
Factor B then binds to the C3b protein (covalently linked to microbial
surface)
Bound factor B is in turn cleaved by a plasma serine protease called factor
D, small fragment called Ba and generating a larger fragment called Bb that
remains attached to C3b.
The C3bBb complex is the alternative pathway C3 convertase, and it
functions to cleave more C3 molecules, thus setting up an amplification
sequence.
C3b generated, both by classical and lectin pathway can form complex with
Bb, and this complex cleaves more C3.
34. Some of the C3b molecules
generated by the alternative
pathway C3 convertase bind
to the convertase itself.
This results in the formation
of a complex containing one
Bb moiety and two molecules
of C3b, which functions as
the alternative pathway C5
convertase that will cleave C5
and initiate the late steps of
complement activation.
35. C3bBb complex formed on mammalian cells- rapidly degraded and
the reaction is terminated by action of regulatory proteins present
on these cells.
Lack of regulatory proteins on microbial cells allows binding and
activation of alternative pathway C3 convertase.
Properdin, can bind to and stabilize the C3bBb complex- attachment
of properdin is favored on microbial surface as opposed to normal
host cells.
Properdin is the only known positive regulator of complement.
36. Properdin -soluble glycoprotein
Plasma concentration -25 μg/ml
• Composed of identical subunits of 53 kDa -
-binding to each other in a head to tail
manner forming cyclic polymers (dimers,
trimers, and tetramers) distributed in
plasma in a ratio of 26:54:20.
Monomer is flexible rod-like structure with
a length of 26 nm and a diameter of 2.5 nm.
The human properdin monomer is 442
amino acids long and is composed of six
non-identical repeats of about 60 amino
acids called thrombospondin type I (TSR)
repeats
Properdin monomer contains an N-
glycosylation site and C-linked
mannosylation sites.
TSRs are also found in cell adhesion
molecules in human blood platelets called
thrombospondins.
37. Known ligands binding to properdin include C3b, C3bB, C3bBb, Sulfatides etc.
Tetramers
Dimers
Trimers
C-linked mannosylation site
38. , ,
This enzyme cleaves C5 (195 kDa), which contains two protein chains,α and β
After binding of C5 to the nonenzymatic C3b component of the convertase,
the amino terminus of the chain is cleaved.
This generates the small C5a fragment (15kDa), which diffuses away, and the
large C5b fragment, which binds to the surface of the target cell .
C5b component- extremely labile and becomes inactive within 2 minutes
unless C6 binds to it and stabilizes its activity
The cleavage also exposes a site on C5b that bind to two new proteins, C6
and C7, to form a multimolecular complex called C5b67
The C5b67 complex can insert into the microbial cell membrane.
40. Once inserted in the surface of an
organism, the complex binds to a
molecule of C8 C9 molecules, then
aggregate with C5b678 complex to
form a tubular structure called
Terminal Complement Complex
(TCC) / Membrane Attack Complex
(MAC).
The completed MAC -has a tubular
form, pore size of 70–100 Å,
consists of a C5b678 complex
surrounded by a poly-C9 complex.
Ions and small molecules – diffuse
freely through the central channel
of the MAC, the cell cannot
maintain its osmotic stability and
is killed by an influx of water and
loss of electrolytes.
41.
42. C3a and C5a-ANAPHYLOTOXINS
These peptides degranulate mast cells
and stimulate platelets to release
vasoactive molecules histamine and
serotonin
Both trigger inflammation throuh their
receptors (C3aR and C5aR)
Powerful chemoattractants for
macrophages and neutrophils
Cause increased vascular
permeability, causing lysosomal
enzyme release from neutrophils and
thromboxane release from
macrophages
C3a and its inactivated derivative C3a
des Arg can kill bacteria
43. Mediated by several circulating and cell
membrane proteins- belong to a family called
regulators of complement activity (RCA)
Complement activation needs to be
regulated for two reasons:
First, low-level complement activation
goes on spontaneously, and if allowed
to proceed, the result can be damage to
normal cells and tissues.
Second, even when complement is
activated where needed, (on microbial
cells or antigen-antibody complexes) it
needs to be controlled -degradation
products of complement proteins can
diffuse to adjacent cells and injure them
44. Properdin and factor H -two key regulatory proteins having opposite
functions in the alternative complement pathway.
Properdin up-regulates the alternative pathway by stabilizing the C3bBb
complex,
Factor H downregulates the pathway by promoting proteolytic degradation
of C3b.
Factor H is mainly produced in the liver, there are several extrahepatic
sources (keratinocytes, skin fibroblasts, ocular tissue, adipose tissue, brain,
lungs, heart, spleen, kidney, muscle, and placenta.)
Neutrophils -major source of properdin, also produced by monocytes, T cells
etc.
Properdin -released by neutrophils from intracellular stores following
stimulation by N-formyl-methionine-leucine-phenylalanine (fMLP) and TNF-α.
Endothelial cells -physiological source for plasma properdin
45. Genes for C4, C2and Factor B located within MHC class III region
Genes for C4BP, CD55, CD35 CD21, CD46 and factor H are linked within the
RCA (regulation of complement activation) cluster
46. C1- inactivator (C1-INH), a glycoprotein that inhibits several proteases in the
classical and lectin pathways.
It is a SERPIN.
The proteolytic activity of C1r and C1s is inhibited by C1 INH.
An autosomal dominant inherited disease called hereditary angioneurotic
edema -deficiency of C1 INH.
Clinical manifestations
Accumulation of edema fluid in the skin and mucosa causing abdominal
pain, vomiting, diarrhea, and potentially life-threatening airway obstruction.
47. If C3b is deposited on the surfaces of normal mammalian cells, it may be
bound by several membrane proteins, including
a) Membrane Cofactor Protein (MCP, or CD46),
b) Type 1 complement receptor (CR1),
c) Decay- Accelerating Factor (DAF), and
d) A plasma protein called factor H.
48. Complement activation is prevented on normal host cells and
permitted on microbes- Cell surfaces rich in sialic acid favor binding
of the regulatory proteins.
DAF is a GPI-linked membrane protein expressed on endothelial cells
and erythrocytes.
Genetic deficiency of an enzyme required to form such protein-lipid
linkages results in failure to express many
glycophosphatidylinositol-linked membrane proteins, including DAF
and CD59 , and causes a disease called paroxysmal nocturnal
hemoglobinuria.
This disease is characterized by recurrent sessions of intravascular
hemolysis, chronic hemolytic anemia and venous thrombosis.
49. Cell-associated C3b is proteolytically degraded by a plasma serine protease called
factor I, which is active only in the presence of MCP, factor H, C4BP, and CR1
Thus, these regulatory host cell proteins promote proteolytic degradation of
complement proteins
Factor I–mediated cleavage of C3b generates fragments- iC3b, C3d, and C3dg, -do not
participate in complement activation but are recognized by receptors on phagocytes
and B lymphocytes.
50. CD59 is a glycophosphatidylinositol-linked protein.
Works by inserting itself into assembling MACs after the membrane
insertion of C5b-8, thereby inhibiting the subsequent addition of C9
molecules
CD59 is present on normal host cells, where it limits MAC formation,
but it is not present on microbes.
51. Formation of MAC is also inhibited by plasma proteins
such as S protein-functions by binding to soluble C5b,6,7
complexes and prevents their insertion into cell
membranes near the site where, complement cascade
was initiated.
52. CR1(CD35)
Found on neutrophils, eosinoplhils, monocytes, macrophages, B-cells
Binds to C3b and C4b as well as,iC3b
Removes immune complexes from circulation
CR1 deficiency-SLE
CR2 (CD21)
Found B cells
Binds to C3d- Breakdown fragment of C3
Stimulate humoral immune responses by enhancing B cell activation by antigen
CR3 (CD11a/CD18)
Found on Nk cells, neutrophils, macrophages
Integrin binds to iC3b
CR4 (CD11c)
Integrin found on neutrophils, T cells, platelets, macrophages
Expressed on dendritic cells and is used as a marker for this cell type
CRIg
Expressed on Kupffer cells.
Integral membrane protein
Binds C3b and iC3b ,is involved in the clearance of opsonized bacteria and other
blood-borne pathogens.
53. The complement system comprises a group of serum proteins, many of
which exist in inactive forms.
54.
55. Thomas J. Kindt, Richard A. Goldsby, Barbara A.
Osborne, Janis Kuby , 6th Edition , W. H. Freeman
-Abbas, Abul K., Andrew HH Lichtman, and
Shiv Pillai, 8th Edition, Elsevier Health Sciences
By Ian R. Tizard 9th Edition, Elsevier Health Sciences
Kenneth Murphy, Casey Weaver, 9th edition, Garland
Science
Kenneth BM Reid and Robert
B Sim, Royal Society of Chemistry.
-Lubna
Kouser, Anuvinder Kaur, Suhair M. Abozaid, Emmanuel Flahaut- Molecular
innate immunity, Frontiers in Immunology, 12 February 2018
Adam Z.
Blatt Sabina Pathan Viviana P. Ferreira, Immunological reviews, Wiley ,26
October 2016
Editor's Notes
Complement proteins are inactive plasma poroteins.It is a complex interacting network that consists of many interacting pattern recognition proteins, proteases serum proteins receptors and regulators
The smaller fragment from C4 cleavage, C4a, is an anaphylatoxin, or mediator of inflammation, which does not participate directly in the complement cascade; (the anaphylatoxins, include the smaller fragments of C4, C3, and C5)
the mechanism
is more like that of the classical pathway, because after initiation,
it proceeds, through the action of C4 and C2, to produce
a C5 convertase
Its function in the complement pathway is similar to that of C1q, which it resembles in structure
The cell walls of Gram-positive and Gram-negative bacteria, are composed of a matrix of proteins, carbohydrates, and lipids in a repetitive array.
3-deoxy D manno octulosonic acid- KDO
Hep=L-glycero-D-Mannoheptose
Basically, lipid A is made up of a ˇ(1→6)-linked glucosamine disaccharide backbone which is mostly phosphorylated at position 1 and 4 of the saccharides and acylated at positions 2 and 3 of each monosaccharide portion (Galanos and Freudenberg, 1993; Homma et al., 1985; Kotani et al., 1985). Usually, lipid A is hexaacetylated, meaning that six acyl chains of variable length are esterified with the disaccharide backbone. Primary acyl chains are directly esterified with the sugar moiety while so-called secondary acyl chains form ester bonds with hydroxyl groups of primary acyl chains
Fucose is a hexose deoxy sugar with the chemical formula C6H12O5. L-fucose (6-deoxy-L-galactose) is a monosaccharide
Its structure and function in the complement pathway is similar to that of C1q.Lectins are diverse and include different families
P-type lectin=pentraxin
C-type lkectin collectin, require calcium for binding to carbohydrates
The MASP-1 and -2 proteins have
structural similarity to C1r and C1s and mimic their activities.
Surfactant proteins, SP-A and SP-D, are collagen-containing C-type (calcium dependent) lectins called collectins, which contribute significantly to surfactant homeostasis and pulmonary immunity. These highly versatile innate immune molecules are involved in a range of immune functions including viral neutralization, clearance of bacteria, fungi and apoptotic and necrotic cells, down regulation of allergic reaction and resolution of inflammation. Their basic structures include a triple-helical collagen region and a C-terminal homotrimeric lectin or carbohydrate recognition domain (CRD).
Natural SP-A is a collagen-like, hydrophilic glycoprotein and constitutes 2-3% (by weight) of total extracellular surfactant. This protein has no direct surface-lowering capability but interacts with phospholipids, carbohydrates, calcium, and cell membrane receptors . It accelerates absorption of phospholipids to an air-liquid interface, regulates secretion and re-uptake of surfactant by alveolar type Il pneumocytes as well as the extracellular transformation of lamellar bodies to tubular myelin (this latter process also requires calcium and SP-B), stimulates phagocytosis of bacteria and viruses by alveolar macraphages, and increases the resistance af surfactant to inhibition by serum proteins.
SP-D is another collagen-like hydrophilic protein present in the airspaces which stimulates the production of free oxygen radicals by alveolar macrophages, but its biophysical role in the surfactant system has not been defined.
SP-B and SP-C are two hydrophobic proteins that together constitute 1-2% (by weight) of total extracellular surfactant. Both SP-B and SP-C enhance the absorption of surfactant phospholipids to an air-liquid interface and addition of hydrophobic proteins to a protein-free artificial surfactant improves its physical and physiological properties. SP-B is an essential constituent of tubular myelin, the extracellular reservoir of surfactant generating the surface film in terminal airspaces.
bind toreceptors on mast cells and blood basophils and induce degranulation, with release of histamine and other pharmacologically active mediators. The anaphylatoxins also induce smooth-muscle contraction and increased vascular permeability. Activation of the complement system thus results in influxes of fluid that carries antibody and phagocytic cells to the site of antigen entry. The activities of these highly reactive anaphylatoxins are regulated by a serum protease called carboxypeptidase N, which cleaves an Arg residue from the C terminus of the molecules, yielding so-called des-Arg forms. The des-Arg forms of C3a and C4a are completely inactive while that of C5a retains about 10% of its chemotactic activity and 1% of its ability to cause smooth muscle contraction.
C3b is the major opsonin of the complement system, although C4b and iC3b also have opsonizing activity. The amplification that occurs with C3 activation results in a coating of C3b on immune complexes and particulate antigens. Phagocytic cells, as well as some other cells, express complement receptors (CR1, CR3, and CR4) that bind C3b, C4b, or iC3b (see Table 13-4).Antigen coated with C3b binds to cells bearing CR1. If the cell is a phagocyte (e.g., a neutrophil, monocyte, or macrophage), phagocytosis will be enhanced (Figure 13-12). Activation of phagocytic cells by various agents, including C5a anaphylatoxin, has been shown to increase the number of CR1s from 5000 on resting phagocytes to 50,000 on activated cells, greatly facilitating their phagocytosis of C3b-coated antigen. Recent studies indicate that complement fragment C3b acts as an adjuvant when coupled with protein antigens. C3b targets the antigen directly to the phagocyte, enhancing the initiation of antigen processing and accelerating specific antibody production
Activation of the complement cascade and the stability of active complement proteins are tightly regulated to prevent complement activation on normal host cells and to limit the duration of complement activation on microbial cells and antigen-antibody complexes
By binding to C3b or C4b, these proteins competitively inhibit binding of other components of C3 convertase, such as Bb of the alternative pathway and C2b of the classical pathway, -blocking further progression of the complement cascade.
Inhibitors of the MAC in the plasma and in host cell membranes ensure that lysis of innocent bystander cells does not occur near the site of complement activation.
Complement activation occurs by the classical, alternative,
or lectin pathways, each of which is initiated differently.
The three pathways converge in a common sequence of
events that leads to generation of a molecular complex that
causes cell lysis.
The classical pathway is initiated by antibody binding to a
cell target; reactions of IgM and certain IgG subclasses activate
this pathway.
Activation of the alternative and lectin pathways is antibody-
independent. These pathways are initiated by reaction
of complement proteins with surface molecules of
microorganisms.
In addition to its key role in cell lysis, the complement system
mediates opsonization of bacteria, activation of inflammation,
and clearance of immune complexes.
Interactions of complement proteins and protein fragments
with receptors on cells of the immune system control
both innate and acquired immune responses.
Because of its ability to damage the host organism, the
complement system requires complex passive and active
regulatory mechanisms.
Clinical consequences of inherited complement deficiencies
range from increases in susceptibility to infection to
tissue damage caused by immune complexes.