This document discusses a study on the hydrophobic anchor known as Lipid A that holds the
Lipopolysaccharide in the outer membrane of gram-negative bacteria. The study identified an enzyme
called tetraacyldisaccharide 1 phosphate 4 kinase (LpxK) that is a potential rate-limiting enzyme in
the Lpx biosynthetic pathway responsible for Lipid A production. The document then discusses the
purification of the LpxK enzyme and notes that the results provide insight into the complexity of
purifying LpxK.