This document provides guidelines for the euthanasia of rodents at the University of Chicago Animal Resources Center. It describes acceptable and conditionally acceptable methods of euthanasia, including carbon dioxide overdose, inhalant anesthetic overdose, and injectable euthanasia agents. It emphasizes the importance of confirming death using a secondary physical method before disposing of rodents. Proper training is required to humanely perform euthanasia techniques and comply with IACUC regulations.