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CARBOHYDRATES
PRESENTED BY
PATTAN SOHAIL
DEPT OF ANALYSIS
BANGALORE
CONTENTS
 General introduction
 Classification
 Qualitative test for carbohydrates
 Digestion
 Absorption
 Metabolism
 Applications
INTRODUCTION
 Cabohydrates are basically polyhydroxy ketones and
aldehydes.
 Carbohydrates are the precursors for Organic
compouns,( fats & amino acids).
 Basic Molecular formula is
CnH2nOn
CLASSIFICATION
carbohydrates
Mono
sachrides
Oligo
sachrides
Poly sachrides
Based on number of carbon atoms monsachrides are
again classified in to following,
Sugar Formula Aldoses Ketoses
Trioses C3H6O3 Glyceraldehyde
s
Dehydroxy
acetone
Tetroses C4H8O4 Erythrose Erthrulose
Pentoses C5H10O5 Xylose
Ribose
Ribulose
Hexoses C6H12O6 Glucose
Galactose
Fructose
Heptoses C7H14O7 Sedoheptulose
Poly sacchrides :- Starch
Oligo sacchrides
Disacchrides
Ex: Sucrose
Lactose
Maltose
Trisacchrides
Ex: raffinose
S NO TEST INFERENCE OBSERVATION
1 MOLISH TEST Reddish violet or Purple ring at
the junction of 2 liquids
Precence of glucose
2 BENEDICTS
TEST
Brick red precipitate Precence of reducing
sugars
3 BARFOEDS
TEST
Scanty brick red ppt Precence of reducing
mono saccharides
4 SELIWANOFFS
TEST
Cherry red colour Precence of ketoses
and aldoses
5 Hydrolysis test for
sucrose
Blue colour indicates
neutralisation
Sucrose is a non-
reducing sugar
6 OSAZONE TEST Needle shaped crystal Fructose,glucose,mann
ose forms similar
crystal
7 BIALS TEST Bluish colour Determination of
pentoses
8 IODINE
REACTION
Yellow-orange---negative
Purple-black----positive
Test for
polysaccharides
DIGESTION
1. Digestion in mouth
Large polysaccharide molecules
(starch,glycogen,dextrins)
Smaller polysaccharide molecules
(glucose,maltose)
Alpha amylase
2. Digestion in stomach
Sucrose
Fructose + glucose
3. Digestion in duodenum
Starch/glycogen
maltose + dextrins and oligosaccharides
Pancreatic
amylase
HCL
3. Digestion in intestine
Maltose Glucose + Glucose
Sucrose Glucose + Fructose
Lactose Glucose + Galactose
Maltase
Sucrase
Lactase
ABSORPTION
absorption involves mainly 3 mechanisms
Facilitated /carrier
mediated diffusion
Active transportPassive diffusion
Features Passive diffusion Facilitated diffusion Active transport
Concentration
gradient
Down the
concentration
gradient from high to
low
Down the
concentration
gradient from high to
low
against the
concentration
gradient from low to
high
Energy
expenditure/used
No energy required No energy required In the form of ATP
Carrier protien/
transporter
Not required Required Required
Speed Slowest mode Fast mode Fast mode
METABOLISM
It is defined as the entire spectrum of chemical reactions
occuring in the living system are reffered
as”METABOLISM”
these are of 3 types
1. Catabaolic pathway
2. Anabolic pathway
3. Amphibolic pathway
Food molecule→simpler molecules→Amphibolic pathway
Protiens,lipids,nucleic acids CO2 + H2O
 CATABOLISM : It is the set of metabolic process that
breakdown large molecules to smaller molecules.It is a
destructive process.
 ANABOLISM : It is set of constructive metabolic
process where the energy released by catabolism is used
to synthesis complex molecules.
Major pathways for carbohydrate metabolism
1. Glycolysis 5. Glycogenolysis
2. Citric acid cycle 6. Galactose/fructose metabolism
3. Gluconeogenesis 7. Uronic acid pathway
4. Glycogenesis
APPLICATIONS
 Used in preparation of simple syrups
 As diluents for preparation of tablets.
 Cellulose is used in the preparation of surgical dressings
 As laxatives - mucilage, lactulose
Antacid - sucralafate.
Diuretics - Mannitol, Sorbitol.
 Dextrose is used in preparation of sterile IV solution.
 Pectin is used in the preparation of anti diarrheal drugs.
THANK YOU

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Chemistry, classification & properties of carbohydrates

  • 2. CONTENTS  General introduction  Classification  Qualitative test for carbohydrates  Digestion  Absorption  Metabolism  Applications
  • 3. INTRODUCTION  Cabohydrates are basically polyhydroxy ketones and aldehydes.  Carbohydrates are the precursors for Organic compouns,( fats & amino acids).  Basic Molecular formula is CnH2nOn
  • 5. Based on number of carbon atoms monsachrides are again classified in to following, Sugar Formula Aldoses Ketoses Trioses C3H6O3 Glyceraldehyde s Dehydroxy acetone Tetroses C4H8O4 Erythrose Erthrulose Pentoses C5H10O5 Xylose Ribose Ribulose Hexoses C6H12O6 Glucose Galactose Fructose Heptoses C7H14O7 Sedoheptulose
  • 6. Poly sacchrides :- Starch Oligo sacchrides Disacchrides Ex: Sucrose Lactose Maltose Trisacchrides Ex: raffinose
  • 7. S NO TEST INFERENCE OBSERVATION 1 MOLISH TEST Reddish violet or Purple ring at the junction of 2 liquids Precence of glucose 2 BENEDICTS TEST Brick red precipitate Precence of reducing sugars 3 BARFOEDS TEST Scanty brick red ppt Precence of reducing mono saccharides 4 SELIWANOFFS TEST Cherry red colour Precence of ketoses and aldoses 5 Hydrolysis test for sucrose Blue colour indicates neutralisation Sucrose is a non- reducing sugar 6 OSAZONE TEST Needle shaped crystal Fructose,glucose,mann ose forms similar crystal 7 BIALS TEST Bluish colour Determination of pentoses 8 IODINE REACTION Yellow-orange---negative Purple-black----positive Test for polysaccharides
  • 8. DIGESTION 1. Digestion in mouth Large polysaccharide molecules (starch,glycogen,dextrins) Smaller polysaccharide molecules (glucose,maltose) Alpha amylase
  • 9. 2. Digestion in stomach Sucrose Fructose + glucose 3. Digestion in duodenum Starch/glycogen maltose + dextrins and oligosaccharides Pancreatic amylase HCL
  • 10. 3. Digestion in intestine Maltose Glucose + Glucose Sucrose Glucose + Fructose Lactose Glucose + Galactose Maltase Sucrase Lactase
  • 11. ABSORPTION absorption involves mainly 3 mechanisms Facilitated /carrier mediated diffusion Active transportPassive diffusion
  • 12. Features Passive diffusion Facilitated diffusion Active transport Concentration gradient Down the concentration gradient from high to low Down the concentration gradient from high to low against the concentration gradient from low to high Energy expenditure/used No energy required No energy required In the form of ATP Carrier protien/ transporter Not required Required Required Speed Slowest mode Fast mode Fast mode
  • 13. METABOLISM It is defined as the entire spectrum of chemical reactions occuring in the living system are reffered as”METABOLISM” these are of 3 types 1. Catabaolic pathway 2. Anabolic pathway 3. Amphibolic pathway Food molecule→simpler molecules→Amphibolic pathway Protiens,lipids,nucleic acids CO2 + H2O
  • 14.  CATABOLISM : It is the set of metabolic process that breakdown large molecules to smaller molecules.It is a destructive process.  ANABOLISM : It is set of constructive metabolic process where the energy released by catabolism is used to synthesis complex molecules. Major pathways for carbohydrate metabolism 1. Glycolysis 5. Glycogenolysis 2. Citric acid cycle 6. Galactose/fructose metabolism 3. Gluconeogenesis 7. Uronic acid pathway 4. Glycogenesis
  • 15. APPLICATIONS  Used in preparation of simple syrups  As diluents for preparation of tablets.  Cellulose is used in the preparation of surgical dressings  As laxatives - mucilage, lactulose Antacid - sucralafate. Diuretics - Mannitol, Sorbitol.  Dextrose is used in preparation of sterile IV solution.  Pectin is used in the preparation of anti diarrheal drugs.