Characterization of microbes for degrading plastic
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This is a formal presentation slide containing info on how microbes can degrade plastics and their character, and variety. It also contains data of different microbes and plastics and their effect on environment.
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Characterization of microbes for degrading plastic
- 1. An overview of characterization of microbes for degrading plastics Abdullah Al-amin Dept. of Biotechnology & Genetic Engineering Roll: ASH1413042M Session: 2013-14 22/11/2018
- 2. Plastics produced by taking oil, coal and natural gas consist of carbon, hydrogen, silicon, oxygen, chloride and nitrogen are polymers that consist of monomers linked together by chemical bonds Comes from the Greek word “plastikos”- means able to be molded in to varied shapes 1
- 3. Types of degradable plastics Photodegradable plastics Compostable plastics UV PA PS PVC HDPE PE Bio-plastics 2 PCL- Polycaprolactone; PBS- Polybutylene succinate; PES- Polyethersulfone; PLA- Polylactic acid ; PE- Polyethylene; PHB- Polyhydroxybutyrate; PVC- Polyvinyl chloride; PA- Polyamide; PS- Polystyrene.
- 4. Thermal properties of plastics 3
- 5. Methods for measuring biodegradability Soil Buried Method Pure Culture Method 4
- 6. Microorganisms with ability of degrading the plastics Bacillus megaterium Pseudomonas stutzeri Ralstonia eutropha Comamonas acidovorans Rhodococcus sp. Pseudomonas putida Aureobasidium pullulans Rhodotorula aurantiaca Aspergillus glaucus Aspergillus niger Aspergillus japonicus Mucor sp. Streptomyces badius Streptomyces setnii Strptomyces viridosporous 5
- 7. Degradation of polyester polyurethane by Aspergillus sp. 6
- 8. Time course of degradation of polyurethane film 7
- 9. Morphological characteristics of filamentous fungi L1- Aspergillus flavus, L2- Paecilomyces sp. , L3- Aspergillus terreus, L4- Penicillium chrysogenum, L5- Cladosporium herbarum, L6- Aspergillus niger. 8
- 10. Degradation of poly (3-Hydroxybutyrate) by filamentous fungi 9 PHA- Polyhydroxyalkanoates
- 11. Productions of polyhydroxybutyrate depolymerase and polycaprolactone depolymerase by Penicillium lilacinus D218 Degradation of polyhydroxybutyrate and polycaprolactone by P. lilucinus D218. The fungus was grown in three media containing 1 g of PHB, 1 g of PCL, and 0.3 g of PHB plus 0.7 g of PCL per liter. 10
- 12. Degradation of Polylactide by Amycolatopsis sp. Renewable resources Poly (lactic acid) Green intermediate Green polymers Lipase 11
- 13. Biodegradation of polyethylene by fungi and Streptomyces species 12
- 14. Thank You
Editor's Notes
- Thermosetting plastics are composed of a network-like structure. polymers remain solid and cannot be melting and modified. Thermoplastics are polymers cannot change in their chemical composition when heated, and can therefore undergo molding multiple times.
- Figure shows the time course of PU film degradation at 30 °C within 28 days. The rate of degradation was slow and 15–20% decrease in weight was observed till the end of experiment. Time course of degradation of PU film by A. fumigatus strain S45. Triangle uninoculated control; Circle-inoculated with strain S45. A gradual loss in weight of PU film within 28 days of incubation with strain S45.
- Poly (3-hydroxybutyrate) (PHB) is receiving much attention as a raw material for biodegradable plastics. This polymer is representative of poly (3-hydroxyalkanoates) (PHAs), which are bacterial reserves of carbon and energy. Environmental conditions for the growth of these bacteria, such as the type and feeding rate of the substrate, pH, temperature, aeration, and nitrogen sources often change the amount and composition of PHAs.
- Strain D218 was statically grown on PHB, PCL, or both and the consumption of these polymers and the depolymerase activities were followed (Fig. 6). In the medium containing PHB, both PHB and PCL depolymerase activities increased gradually with the decrease of PHB and reached a constant value after 6 d. Strain D218 did not hydrolyze PCL as quickly as it did PHB, and about 90% of the polymer was still present in the medium 10 d after inoculation. As compared with PHB, PCL was a poor substrate for the fungal growth, resulting in lower productivity of PHB depolymerase. In the presence of both PHB and PCL, strain D218 seemed to hydrolyze PCL slowly after preferable consumption of PHB for 4 d and both depolymerase activities were lower than those grown on PHB alone.
- The isolation of Amycolatopsis sp. is an effective strain for the treatment of PLA plastic waste. Polylactide or poly (lactic acid) (PLA) was formerly known as a hydrolyzable and unstable polyester with limited use as a biodegradable material. PLA is polymerized from lactic acid, which can be prepared effectively by fermentation with renewable resources such as starchy materials and cellulose. Liquid cultures of an Amycolatopsis sp. were carried out with two kinds of PLA film with different L-lactic acid contents. PLA with 100% L-lactic acid content and PLA with 94% L-lactic acid content were collected.
- The ability of fungi and Streptomyces species to attack degradable plastics was investigated.