Biology is the study of life, which examines living organisms such as their cellular structure, energy use, growth, response to the environment, and evolution. It uses the scientific method of making observations, asking questions, generating hypotheses, and performing tests to build theories and gain an understanding of the natural world. Some key themes in biology include evolution, inheritance, cells, biological classification, bioenergetics, homeostasis, and ecosystems. Studying biology helps address needs of human populations and biodiversity challenges while also raising controversial issues around topics like endangered species and biomedical research.