This document discusses various methods for counting cells, including hemocytometers, automated cell counters, flow cytometers, spectrophotometry, and image analysis. Hemocytometers use a gridded glass slide to manually count cells under a microscope in a small volume of cell suspension. Flow cytometers are frequently used and can detect labeled intracellular components as cells flow past a laser, while spectrophotometry measures light absorbance as a proxy for cell density. Imaging analysis uses algorithms to count cells from high-quality microscope images.
Flow cytometry is a technique used in Cell Biology to analyze and measure the volume of cells suspended in a liquid with streamline flow, exposed to a laser beam.
In this presentation, you will learn everything you need to know to get started on flow cytometry. You will learn how it works, how to choose the right antibody for FACS, how to gate, and many more.
Flow cytometry is a technique used in Cell Biology to analyze and measure the volume of cells suspended in a liquid with streamline flow, exposed to a laser beam.
In this presentation, you will learn everything you need to know to get started on flow cytometry. You will learn how it works, how to choose the right antibody for FACS, how to gate, and many more.
Fluorescence- Activated Cell Sorter is a powerful technique used in cell sorting, cell-cycle analysis etc.
The presentation gives a basic understanding of the principle of FACS, instrumentation, interpretation of results, applications, how to do cell-cycle analysis using FACS and various troubleshooting tips.
the slideshare is been made to get knowledge about flow cytometry it's introduction, working, construction mainly components used in the flow cytometry and its application to use
haemocytometer (AARTI PAL).pptx M PharmacyAartiPal23
Haemocytometer (Neubauer's chamber): to calculate the density of cells in suspensions. Counting rules, coulter counter method, cryopreservation, Charaterization of cell lines , Addition of cryoprotectants, Application of animal cell culture,
Fluorescence- Activated Cell Sorter is a powerful technique used in cell sorting, cell-cycle analysis etc.
The presentation gives a basic understanding of the principle of FACS, instrumentation, interpretation of results, applications, how to do cell-cycle analysis using FACS and various troubleshooting tips.
the slideshare is been made to get knowledge about flow cytometry it's introduction, working, construction mainly components used in the flow cytometry and its application to use
haemocytometer (AARTI PAL).pptx M PharmacyAartiPal23
Haemocytometer (Neubauer's chamber): to calculate the density of cells in suspensions. Counting rules, coulter counter method, cryopreservation, Charaterization of cell lines , Addition of cryoprotectants, Application of animal cell culture,
Direct methods of measurement of microbial growth includes various methods of enumeration of both viable and non viable cell also includes growth curve. Helpful for UG and PG programs of microbiology
Atlas on bethesda system for reporting cervical cytologyAshish Jawarkar
This is an atlas with more nearly 100 images, authentic taken from NCI web atlas. Useful to understand and report pap smears. The subject has been presented in a way which will help students reproduce in exams.
The technique of flow cytometry is used to evaluate cells for a number of functions, such as cell counting, phenotyping, cell cycle analysis, and viability.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
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(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
2. Introduction
Numerous procedures in biology and medicine
require the counting of cells.
In medicine RBCs and WBCs
concentration of pathogens in Body fluids can
reveal information about the progress of an
infectious disease
3. Methods
Different mehod of cell count are available:
– Hemocytometers
– Automated Cell Counters
– Flow Cytometers
– Spectrophotometry
– Image Analysis
4. Hemocytometers
gridded chamber on a glass slide
1 mm x 1 mm chambers
subdivided into 0.05 mm x 0.05 mm
sample of cell suspension
cell suspension counting chamber by capillary action.
microscope is focused
1 mm2, 100 nl area of counting chamber
Repeated
results are averaged
5. Flow cytometer
most frequently used
fluorescence detection technologies
detect labelled intracellular components
cells flow in a narrow stream
front of a laser beam
6. Spectrophotometry
not a reliable method
cell density
measure absorbance of light
culture is placed in a transparent cuvette
OD is proportional to the biomass in the cell suspension
7. Plating and CFU counting
slowest method
gives an accurate estimate
cells can be simply plated on a petri dish
each cell will give rise to a single colony or Colony
Forming Unit (CFU)
volume of culture concentration can be
calculated
