This document provides information about constructing cDNA libraries. It discusses the differences between genomic and cDNA libraries, including that genomic libraries contain promoters, introns, intergenic regions, and non-expressed genes while cDNA libraries only contain expressed genes, transcription start sites, and open reading frames. It also outlines the steps of purifying mRNA and synthesizing cDNA from mRNA to construct a cDNA library. Finally, it poses questions about differences between genomic and cDNA libraries, purification of mRNA, cDNA synthesis, and the life cycle of bacteriophages.

1. 1
cDNA Library
Asst. Prof. Om M. Bagade
M. Pharm, D.
I. P. L
Department of
Pharmaceutics

2. Objective:
To make students understand regarding the Genetic
Engineering, cDNA library.
Outcome:
Students will familiar with the different
Construction and analysis of a genomic DNA
library methods,Purification of mRNA &
Synthesis of cDNA from mRNA

3. Construction and analysis
of a genomic DNA library

4. Construction of a
cDNA library

5. Differences between a genomic and cDNA library
Genomic Library
Promoters
Introns
Intergenic
Non-expressed genes
cDNA Library
Expressed genes
Transcription start sites
Open reading frames (ORFs)
Splice points

6. Purification of mRNA

7. Synthesis of cDNA from mRNA

8. SfiI digestion sites

9. SP Primer
XP Primer
A.f. insert
Is there an insert?

10. Life cycle of a Bacteriophage

11. Constructing a genomic library in phage

12. Constructing a genomic library in cosmids

13. Constructing a genomic library in YACs

14. What Do you Think about following………..
 Differences between a genomic and cDNA
library?
 Give details about Purification of mRNA?
 Explain Synthesis of cDNA from mRNA?
 What do you mean by Life cycle of a
Bacteriophages?