Genetic libraries
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Genetic libraries allow researchers to study genes and proteins from organisms. There are two main types of genetic libraries: genomic libraries and cDNA libraries. To build a genomic library, genomic DNA is extracted from an organism and cleaved into fragments using restriction enzymes. These fragments are then inserted into plasmids which are introduced into bacteria, allowing amplification of the complete genome. A cDNA library focuses only on expressed genes. mRNA is extracted and converted to cDNA, then inserted into lambda phages which infect bacteria for replication. The library can then be screened using probes to isolate specific DNA fragments of interest.
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Construction of genomic library in lambda
Construction of genomic library in lambda phage.
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Genomiclibrary 151004020241-lva1-app6891
Genomic libraries contain DNA fragments representing an organism's entire genome. They are created through molecular cloning by isolating genomic DNA, fragmenting it, inserting the fragments into vectors, and introducing the vectors into bacteria. This collection of cloned DNA fragments comprises the genomic library and allows researchers to identify and study specific genes of interest. Shotgun sequencing is an alternative approach that involves randomly fragmenting genomic DNA and determining the nucleotide sequence of each fragment to reconstruct the full genome sequence computationally.
Genomic library
The document discusses genomic libraries. A genomic library contains DNA fragments representing the entire genome of an organism. It is constructed by inserting fragments of partially digested genomic DNA into vectors like plasmids or bacteriophages, which are then introduced into bacteria or yeast. The bacteria replicate and produce clones containing fragments of the original genome, theoretically representing all its sequences. Genomic libraries allow researchers to explore genomic structure and function, map genomes to identify gene locations, and clone DNA segments.
genomic library
The document discusses genomic libraries, which are collections of DNA fragments from an organism's genome stored in host cells. To create a genomic library, DNA is extracted from cells, cut into fragments, and inserted into vectors like plasmids. The vectors are then taken up by host cells, allowing the fragments to be replicated and retrieved for analysis. Genomic libraries have been important for whole genome sequencing projects and studying gene function, regulation, and mutations. The document also discusses cDNA libraries, vectors, and techniques for sequencing DNA like Sanger sequencing and next-generation sequencing.
Genomic library
Genomic libraries are collections of bacteria that have been engineered to contain the entire DNA of an organism. DNA from the organism is cut into fragments and inserted into cloning vectors like plasmids within bacteria. Together the bacteria hold the complete genome. Genomic libraries allow researchers to isolate and study specific DNA sequences using probes, map genomes, and clone DNA segments for various research purposes like genetic modification of crops. They are constructed by extracting, cutting, and ligating an organism's DNA into vectors within host bacteria. Different vectors have varying capacities to hold DNA fragments, ranging from plasmids at 15kb to yeast artificial chromosomes at up to 2,000kb.
Construction of gene library
In humans, approximately 25,000 genes exit among the 3 billion base pairs of DNA in the genome.
To study anyone of these genes, a researcher first isolates it from all of the other genes in an organisms DNA.
One isolation method has a relatively long history and involves the construction of a DNA library
When a gene is identified and copied, it is said to have been “cloned”
DNA Library
A DNA library is a collection of DNA fragments cloned into vectors that together represent the entire genome of an organism. There are two main types of libraries: genomic DNA libraries, which contain all the organism's DNA including coding and non-coding regions, and cDNA libraries, which contain only the expressed coding regions. To create a library, DNA is extracted from cells, cut into fragments, and inserted into vectors which are then introduced into host cells. This allows amplification and isolation of specific clones for analysis.
Gene Libarries
DNA library is a collection of DNA, three types of recombinant DNA libraries are present
1) Genomic library
2) Chromosomal library
3) Complementary DNA library
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Genomic libraries contain DNA fragments representing an organism's entire genome. They are created through molecular cloning by isolating genomic DNA, fragmenting it, inserting the fragments into vectors, and introducing the vectors into bacteria. This collection of cloned DNA fragments comprises the genomic library and allows researchers to identify and study specific genes of interest. Shotgun sequencing is an alternative approach that involves randomly fragmenting genomic DNA and determining the nucleotide sequence of each fragment to reconstruct the full genome sequence computationally.
Genomic library
The document discusses genomic libraries. A genomic library contains DNA fragments representing the entire genome of an organism. It is constructed by inserting fragments of partially digested genomic DNA into vectors like plasmids or bacteriophages, which are then introduced into bacteria or yeast. The bacteria replicate and produce clones containing fragments of the original genome, theoretically representing all its sequences. Genomic libraries allow researchers to explore genomic structure and function, map genomes to identify gene locations, and clone DNA segments.
genomic library
The document discusses genomic libraries, which are collections of DNA fragments from an organism's genome stored in host cells. To create a genomic library, DNA is extracted from cells, cut into fragments, and inserted into vectors like plasmids. The vectors are then taken up by host cells, allowing the fragments to be replicated and retrieved for analysis. Genomic libraries have been important for whole genome sequencing projects and studying gene function, regulation, and mutations. The document also discusses cDNA libraries, vectors, and techniques for sequencing DNA like Sanger sequencing and next-generation sequencing.
Genomic library
Genomic libraries are collections of bacteria that have been engineered to contain the entire DNA of an organism. DNA from the organism is cut into fragments and inserted into cloning vectors like plasmids within bacteria. Together the bacteria hold the complete genome. Genomic libraries allow researchers to isolate and study specific DNA sequences using probes, map genomes, and clone DNA segments for various research purposes like genetic modification of crops. They are constructed by extracting, cutting, and ligating an organism's DNA into vectors within host bacteria. Different vectors have varying capacities to hold DNA fragments, ranging from plasmids at 15kb to yeast artificial chromosomes at up to 2,000kb.
Construction of gene library
In humans, approximately 25,000 genes exit among the 3 billion base pairs of DNA in the genome.
To study anyone of these genes, a researcher first isolates it from all of the other genes in an organisms DNA.
One isolation method has a relatively long history and involves the construction of a DNA library
When a gene is identified and copied, it is said to have been “cloned”
DNA Library
A DNA library is a collection of DNA fragments cloned into vectors that together represent the entire genome of an organism. There are two main types of libraries: genomic DNA libraries, which contain all the organism's DNA including coding and non-coding regions, and cDNA libraries, which contain only the expressed coding regions. To create a library, DNA is extracted from cells, cut into fragments, and inserted into vectors which are then introduced into host cells. This allows amplification and isolation of specific clones for analysis.
Gene Libarries
DNA library is a collection of DNA, three types of recombinant DNA libraries are present
1) Genomic library
2) Chromosomal library
3) Complementary DNA library
DNA Libraries PPT
DNA Libraries are collection of fragments of DNA cloned to a vector so that researchers can easily identify and isolate a particular gene of interest for future use.
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This document discusses gene and DNA libraries. It defines a gene library as a collection of DNA fragments cloned into vectors. There are two main types of libraries: genomic libraries, which contain total genomic DNA from an organism, and cDNA libraries, which contain cloned cDNA fragments representing part of an organism's transcriptome. The document outlines the steps to construct both genomic and cDNA libraries, including fragmenting DNA, ligating it into a vector, transforming the vector into host cells, and screening the library. Gene libraries have applications such as discovering novel genes, studying gene function, and producing proteins.
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The document summarizes the process of constructing genomic and cDNA libraries. A genomic library contains total genomic DNA from an organism, including exons and introns. Genomic DNA is extracted, fragmented, and inserted into vectors which are then introduced into host cells. Each cell contains a different DNA fragment. A cDNA library contains only expressed genes as cDNA, excluding introns. mRNA is extracted and reverse transcribed to cDNA, which is then inserted into vectors. Both library types are useful tools for sequencing and studying gene expression.
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This document provides information about genomic libraries and PCR. It discusses what genomic libraries are, the types of DNA libraries, and the steps to construct a genomic library. Key aspects include extracting and purifying DNA, cutting it into fragments with restriction enzymes, inserting fragments into plasmids, and adding the recombinant plasmids to bacteria to create the library. Probes are used to screen libraries by hybridizing to identify clones containing sequences similar to the probe. Genomic libraries allow researchers to explore and map genomes to further understand organisms. PCR is also summarized, including its invention and the components and steps involved in the process.
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This document describes DNA libraries and the process of creating them. It discusses genomic libraries, which contain an organism's entire genome, and cDNA libraries, which contain copies of mRNA. The key steps to create a library are: 1) isolating and fragmenting DNA, 2) cloning the fragments into vectors, 3) transforming the vectors into host cells, 4) multiplying and screening the clones to identify fragments of interest. cDNA libraries are useful for studying eukaryotic genes as they remove non-coding regions.
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Genomic DNA and cDNA libraries are two ways to obtain genomic information. [1] A genomic DNA library contains the entire human genome, including exons and introns. [2] A cDNA library contains only the expressed portions of the genome (exons). [3] λ-phage is commonly used as the vector to generate these libraries due to its ability to clone large DNA fragments and be packaged in large numbers.
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This document discusses genomic and cDNA libraries. A genomic library contains all DNA sequences from an organism's genome cloned into vectors, while a cDNA library contains mRNA sequences cloned into vectors. Key steps in constructing a genomic library are isolating genomic DNA, fragmenting it, inserting fragments into vectors, and introducing the vectors into bacteria to create a collection of cloned genomic fragments. A cDNA library is made from mRNA and will lack non-coding introns and regulatory elements found in genomic DNA. The document outlines the process of constructing cDNA libraries, which involves isolating mRNA, reverse transcribing it to cDNA, and inserting cDNA fragments into vectors before introducing them into bacteria. Both library types are useful research tools for isolating and studying genes.
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A DNA library is a collection of DNA fragments that have been cloned into vectors. DNA libraries allow researchers to isolate and study specific DNA fragments of interest. To create a genomic library, DNA is extracted from an organism, cut into fragments, inserted into vectors, and introduced into host bacteria to generate clones containing all the organism's DNA sequences. This library can then be screened to identify and study particular genes. DNA libraries provide an efficient way to store, isolate, and analyze DNA sequences.
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DNA Libraries
DNA libraries contain cloned DNA fragments from an organism's genome or cDNA from mRNA. Genomic libraries represent entire genomes, while cDNA libraries represent expressed genes. Genomic libraries are constructed by isolating chromosomal DNA, fragmenting it, cloning the fragments into vectors, and screening clones. cDNA libraries are constructed by synthesizing cDNA from mRNA, cloning the cDNA into vectors, and screening clones. Both library types allow identification of specific DNA or cDNA sequences through hybridization or other screening methods.
C dna
This document discusses cDNA and genomic libraries. It defines cDNA as complementary DNA synthesized from mRNA, and genomic libraries as collections of DNA fragments representing an organism's entire genome. It describes how cDNA libraries are constructed by synthesizing cDNA from purified mRNA, and genomic libraries by partially digesting genomic DNA and inserting fragments into cloning vectors. It also explains how these libraries are screened using probes to identify clones containing genes of interest or encoding specific proteins.
Dna library
A DNA library is a collection of cloned restriction fragments of the DNA of an organism.
Two kinds of libraries will be discussed: genomic libraries and complementary DNA (cDNA) libraries.
Genomic libraries ideally contain a copy of every DNA nucleotide sequence in the genome.
In contrast, cDNA libraries contain those DNA sequences that appear as mRNA molecules, and these differ from one cell type to another.
Dna libraries
Genomic, nuclear, organelle, and cDNA libraries are created by isolating DNA through cell lysis and digestion. Genomic DNA is purified from protein complexes using detergent, protease, and phenol-chloroform extraction before generating fragments of a suitable size through cloning vectors. mRNA is isolated and converted to cDNA, which is then ligated into a vector and transformed into a host to generate colonies containing the cloned DNA library. Different vectors have varying cloning capacities.
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Theoretically, phage display is an exogenous gene expression method which the gene encoding the interest protein is inserted into bacteriophage coat protein gene then displaying the interest protein on the phage surfaces, resulting in a connection between genotype and phenotype.https://www.creative-biolabs.com/phage-display-service.html
Complementary dna library
A cDNA library contains cloned cDNA fragments that are produced from mRNA to represent only the expressed genes of an organism. The construction of a cDNA library involves three main steps: mRNA extraction and purification, cDNA construction by reverse transcription of mRNA, and insertion of cDNA into host cells like E.coli for storage and expression. cDNA libraries are useful because they contain less information by removing non-coding regions, which allows eukaryotic genes to be expressed in prokaryotes that lack machinery for intron removal.
cDNA library construction
The complete genome sequences of a number of organisms, including mammals, have recently become available because of rapid advances in DNA sequencing technology. Nevertheless, the analysis of transcripts still plays a crucial role in bridging the gap between the genome and the proteome, particularly in mammals. Therefore, as a method for the analysis of transcripts, cDNA library construction is crucial, even in the post-genome sequencing era.
https://www.creative-biogene.com/Services/cDNA-Library-Construction-Service
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- Restriction enzymes cut DNA at specific recognition sequences known as restriction sites. They are used in gene cloning and other applications.
- There are three main types of restriction enzymes - Type I, II, and III - which differ in their structure, cofactors required, recognition sequences, and cleavage sites.
- Restriction enzymes play a role in bacterial defense against bacteriophages by cleaving invading phage DNA. The host bacterial DNA is protected by methylation.
- DNA cloning uses restriction enzymes to cut out a gene segment and insert it into a vector for replication in a host cell. This allows production of multiple copies of
cDNA Library Construction
Creation of a cDNA library starts with mRNA instead of DNA. Messenger RNA carries encoded information from DNA to ribosomes for translation into protein. To create a cDNA library, these mRNA molecules are treated with the enzyme reverse transcriptase, which is used to make a DNA copy of an mRNA (i.e., cDNA). A cDNA library represents a sampling of the transcribed genes, but a genomic library includes untranscribed regions.
genomic library.pptx
The document discusses genomic libraries. A genomic library is a collection of DNA fragments that make up the full genome of an organism. To create a genomic library, DNA is extracted from cells and digested with restriction enzymes to cut it into fragments. These fragments are inserted into vectors, such as plasmids, and introduced into bacteria. The bacteria containing the recombinant DNA molecules represent the genomic library. Genomic libraries are important tools for whole genome sequencing and identifying novel genes.
Principle and procedure for making Genomic library and cDNA library.pptx
This document presents information on genomic and cDNA libraries. It begins by defining a gene library as a collection of DNA sequences from an organism cloned into a vector. There are two main types - genomic libraries containing all sequences from the genome, and cDNA libraries containing sequences represented in mRNA. The document then describes the principles, vectors, and procedures used to construct each type of library. Key steps include fragmenting genomic DNA, ligating fragments into vectors, and amplifying the libraries in host cells for genomic libraries, and reverse transcribing mRNA and ligating cDNA into vectors for cDNA libraries. The advantages and disadvantages of each approach are also summarized.
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This document discusses genomic and cDNA libraries. A genomic library contains all DNA sequences from an organism's genome cloned into vectors, while a cDNA library contains mRNA sequences cloned into vectors. Key steps in constructing a genomic library are isolating genomic DNA, fragmenting it, inserting fragments into vectors, and introducing the vectors into bacteria to create a collection of cloned genomic fragments. A cDNA library is made from mRNA and will lack non-coding introns and regulatory elements found in genomic DNA. The document outlines the process of constructing cDNA libraries, which involves isolating mRNA, reverse transcribing it to cDNA, and inserting cDNA fragments into vectors before introducing them into bacteria. Both library types are useful research tools for isolating and studying genes.
Genomic library construction
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Dna library
A DNA library is a collection of cloned restriction fragments of the DNA of an organism.
Two kinds of libraries will be discussed: genomic libraries and complementary DNA (cDNA) libraries.
Genomic libraries ideally contain a copy of every DNA nucleotide sequence in the genome.
In contrast, cDNA libraries contain those DNA sequences that appear as mRNA molecules, and these differ from one cell type to another.
Dna libraries
Genomic, nuclear, organelle, and cDNA libraries are created by isolating DNA through cell lysis and digestion. Genomic DNA is purified from protein complexes using detergent, protease, and phenol-chloroform extraction before generating fragments of a suitable size through cloning vectors. mRNA is isolated and converted to cDNA, which is then ligated into a vector and transformed into a host to generate colonies containing the cloned DNA library. Different vectors have varying cloning capacities.
Phage display technology
Theoretically, phage display is an exogenous gene expression method which the gene encoding the interest protein is inserted into bacteriophage coat protein gene then displaying the interest protein on the phage surfaces, resulting in a connection between genotype and phenotype.https://www.creative-biolabs.com/phage-display-service.html
Complementary dna library
A cDNA library contains cloned cDNA fragments that are produced from mRNA to represent only the expressed genes of an organism. The construction of a cDNA library involves three main steps: mRNA extraction and purification, cDNA construction by reverse transcription of mRNA, and insertion of cDNA into host cells like E.coli for storage and expression. cDNA libraries are useful because they contain less information by removing non-coding regions, which allows eukaryotic genes to be expressed in prokaryotes that lack machinery for intron removal.
cDNA library construction
The complete genome sequences of a number of organisms, including mammals, have recently become available because of rapid advances in DNA sequencing technology. Nevertheless, the analysis of transcripts still plays a crucial role in bridging the gap between the genome and the proteome, particularly in mammals. Therefore, as a method for the analysis of transcripts, cDNA library construction is crucial, even in the post-genome sequencing era.
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This document discusses restriction enzymes and DNA restriction. Some key points:
- Restriction enzymes cut DNA at specific recognition sequences known as restriction sites. They are used in gene cloning and other applications.
- There are three main types of restriction enzymes - Type I, II, and III - which differ in their structure, cofactors required, recognition sequences, and cleavage sites.
- Restriction enzymes play a role in bacterial defense against bacteriophages by cleaving invading phage DNA. The host bacterial DNA is protected by methylation.
- DNA cloning uses restriction enzymes to cut out a gene segment and insert it into a vector for replication in a host cell. This allows production of multiple copies of
cDNA Library Construction
Creation of a cDNA library starts with mRNA instead of DNA. Messenger RNA carries encoded information from DNA to ribosomes for translation into protein. To create a cDNA library, these mRNA molecules are treated with the enzyme reverse transcriptase, which is used to make a DNA copy of an mRNA (i.e., cDNA). A cDNA library represents a sampling of the transcribed genes, but a genomic library includes untranscribed regions.
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This document discusses genomic DNA libraries and cDNA libraries. It provides information on:
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More from Jim Taylor, ASQ-CRE, CPE, CPMM
Hepititis a
Hepatitis A is caused by the hepatitis A virus (HAV), which has a single-stranded RNA genome surrounded by an icosahedral capsid. The virus is primarily spread when an uninfected person ingests food or water contaminated with the feces of an infected person. There is no specific treatment for hepatitis A, but improved sanitation, food safety, and immunization are effective ways to prevent the disease.
Bt corn r2 (2)carla
Bacillus thuringiensis is a bacterium that produces proteins toxic to certain insects. The first Bt corn was registered with the EPA in 1995. Bt corn provides over 99% control of European corn borers through Cry proteins in plant tissues that form pores in the insect's gut, causing paralysis and death.
How to develop pm tasks for a machine
1) The document discusses developing a machine-centered preventative maintenance program that optimizes machine health rather than focusing on individual technologies or minimizing costs.
2) It recommends taking a systematic approach of first identifying possible machine failures, determining which are most significant, trying to avoid failures, getting early warnings of failures that can't be avoided, tailoring tests to detect those warnings, and collecting all machine health data at key decision points.
3) This machine-centered approach aims to better assess overall machine health, catch issues earlier, defer unnecessary overhauls, and improve failure management compared to traditional technology-centered programs.
Six steps to motor management webcast
The document outlines a 6 step motor management plan to minimize costs, maintain reliability, and receive early warnings of failures. The steps include: 1) creating a motor inventory and tracking program, 2) developing repair/replace guidelines, 3) preparing a spares inventory, 4) establishing purchasing specifications, 5) developing repair specifications, and 6) implementing planned maintenance. The overall goals are to reduce energy costs through proper sizing and efficiencies, maintain uptime, and detect potential issues before failures occur.
Fracas - Failure Scene Investigation
When a failure happens, you want to find out why and take action to prevent future occurrence. To do this you must capture as much evidence - verbal, physics, digital - as possible. This presentation discusses that.
Machine centered for noria louisville 07
This document outlines a six step process to take a machine-centered approach to machinery healthcare:
1. Identify possible failure modes of the machine.
2. Determine which failures are most significant based on frequency and consequences.
3. Figure out ways to avoid failures through adjustments, lubrication, or preventative replacements.
4. For failures that can't be avoided, identify ways to get early warnings through process parameters, inspections, or technology.
5. Tailor a suite of tests to monitor for early warnings of significant failures.
6. Collect all monitoring information at a single decision point to determine if maintenance is needed.
Taking this approach optimizes the health of each machine
The key to improving your availability is fracas
The document discusses how to implement a Failure Reporting, Analysis, and Corrective Action System (FRACAS) to improve equipment availability. It recommends capturing comprehensive failure data, conducting a root cause analysis to identify the underlying causes, and developing a corrective action plan. Implementing FRACAS effectively can help eliminate most failures through preventative actions, resulting in increased availability and decreased costs while keeping customers satisfied. The key is for maintenance professionals to have the right system and accountability to track failures from data collection through implementing solutions.
Three primary steps in maintenance reliability engineering
The document outlines three primary steps in maintenance reliability engineering:
1. Measure availability and identify failure-prone equipment by calculating metrics like MTBF, MTTR, and MLDT.
2. Perform root cause failure analysis to determine the underlying causes of failures and their costs.
3. Develop and implement corrective job plans using reliability centered maintenance principles to eliminate causes and manage failures, aimed at increasing availability and reducing costs over time.
Dimensions of Change
Mark is the maintenance manager who wants to implement a CMMS to digitize their maintenance processes but knows some employees may resist change. The document outlines the three dimensions of change that Mark needs to consider to maximize success which are getting the organizational culture, individual employees, and infrastructure supporting the new system all on board with the transition. It provides advice on assessing where people are in accepting change and guidance on moving them along the adoption curve.
More from Jim Taylor, ASQ-CRE, CPE, CPMM (9)
Three primary steps in maintenance reliability engineering
Three primary steps in maintenance reliability engineering
Recently uploaded
SAR of Medicinal Chemistry 1st by dk.pdf
In this presentation include the prototype drug SAR on thus or with their examples .
Syllabus of Second Year B. Pharmacy
2019 PATTERN.
The binding of cosmological structures by massless topological defects
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
Equivariant neural networks and representation theory
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
Katherine Romanak - Geologic CO2 Storage.pdf
Presented at June 6-7 Texas Alliance of Groundwater Districts Business Meeting
Randomised Optimisation Algorithms in DAPHNE
Slides from talk:
Aleš Zamuda: Randomised Optimisation Algorithms in DAPHNE .
Austrian-Slovenian HPC Meeting 2024 – ASHPC24, Seeblickhotel Grundlsee in Austria, 10–13 June 2024
https://ashpc.eu/
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
Bob Reedy - Nitrate in Texas Groundwater.pdf
Presented at June 6-7 Texas Alliance of Groundwater Districts Business Meeting
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
Direct Seeded Rice - Climate Smart Agriculture
Direct Seeded Rice - Climate Smart AgricultureInternational Food Policy Research Institute- South Asia Office
PPT on Direct Seeded Rice presented at the three-day 'Training and Validation Workshop on Modules of Climate Smart Agriculture (CSA) Technologies in South Asia' workshop on April 22, 2024.
Micronuclei test.M.sc.zoology.fisheries.
Current Ms word generated power point presentation covers major details about the micronuclei test. It's significance and assays to conduct it. It is used to detect the micronuclei formation inside the cells of nearly every multicellular organism. It's formation takes place during chromosomal sepration at metaphase.
The debris of the ‘last major merger’ is dynamically young
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
Pests of Storage_Identification_Dr.UPR.pdf
InIndia-post-harvestlosses-unscientificstorage,insects,rodents,micro-organismsetc.,accountforabout10percentoftotalfoodgrains
Graininfestation
Directdamage
Indirectly
•theexuviae,skin,deadinsects
•theirexcretawhichmakefoodunfitforhumanconsumption
About600speciesofinsectshavebeenassociatedwithstoredgrainproducts
100speciesofinsectpestsofstoredproductscauseeconomiclosses
Compexometric titration/Chelatorphy titration/chelating titration
Classification
Metal ion ion indicators
Masking and demasking reagents
Estimation of Magnisium sulphate
Calcium gluconate
Complexometric Titration/ chelatometry titration/chelating titration, introduction, Types-
1.Direct Titration
2.Back Titration
3.Replacement Titration
4.Indirect Titration
Masking agent, Demasking agents
formation of complex
comparition between masking and demasking agents,
Indicators/Metal ion indicators/ Metallochromic indicators/pM indicators,
Visual Technique,PM indicators (metallochromic), Indicators of pH, Redox Indicators
Instrumental Techniques-Photometry
Potentiometry
Miscellaneous methods.
Complex titration with EDTA.
快速办理(UAM毕业证书)马德里自治大学毕业证学位证一模一样
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Authoring a personal GPT for your research and practice: How we created the Q...
Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
Recently uploaded (20)
Basics of crystallography, crystal systems, classes and different forms
Basics of crystallography, crystal systems, classes and different forms
The binding of cosmological structures by massless topological defects
The binding of cosmological structures by massless topological defects
Equivariant neural networks and representation theory
Equivariant neural networks and representation theory
GBSN - Biochemistry (Unit 6) Chemistry of Proteins
GBSN - Biochemistry (Unit 6) Chemistry of Proteins
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
aziz sancar nobel prize winner: from mardin to nobel
aziz sancar nobel prize winner: from mardin to nobel
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
The debris of the ‘last major merger’ is dynamically young
The debris of the ‘last major merger’ is dynamically young
Compexometric titration/Chelatorphy titration/chelating titration
Compexometric titration/Chelatorphy titration/chelating titration
Authoring a personal GPT for your research and practice: How we created the Q...
Authoring a personal GPT for your research and practice: How we created the Q...
Genetic libraries
- 1. Genetic Libraries Part 1: How to build them
- 2. WHY DO WE WANT ONE? WHAT GOOD IS IT? IS IT WORTH THE EFFORT?
- 3. Suppose we want to study a particular gene or protein from an organism.
- 4. For example beta globulin
- 5. We will need lots of mRNA and protein
- 6. How do we get sufficient material for our experiments?
- 7. PCR works for DNA, not RNA.
- 8. 1st step: Build a genetic library of that organism
- 10. Genomic
- 11. cDNA
- 12. One type of library is a Genomic library
- 13. 1. Get genome – DNA
- 14. Add some Restriction Enzymes to the mix
- 15. These will cleave the DNA into small pieces
- 16. All the fragments differ in size
- 17. So we can separate them with Gel Electrophoresis
- 18. Then we need to amplify them
- 19. Add DNA to plasmid
- 21. Then you have a DNA library in many pieces
- 22. But when combined, contain the complete genome
- 23. Another type of library is a cDNA library
- 24. Just looks at expressed genes
- 25. A cDNA library is Tissue specific
- 26. Start with cell of interest (COI)
- 27. Start by isolating mRNA.
- 28. Use reverse transcriptase to make cDNA
- 29. Add restriction ends but don’t cut cDNA
- 30. Next we’ll need a vector -- lambda phage
- 32. Add cohesive ends to DNA segments
- 33. And insert into phage
- 34. Each phage containing DNA is allowed to infect an E. coli.
- 35. Get many copies when lise.
- 36. Lambda phages live a long time so good storage.
- 37. Genetic Libraries Part 2: How to use them (coming soon)
- 40. Prepare a petri dish with an E. coli lawn
- 42. Phage plaques form on the lawn
- 43. Make radioactive probe with complimentary DNA probe
- 44. Isolate individual plaque (cDNA)
- 45. Use re to cut up cDNA small enough to fit in vector but not so small that genes get too cut up.
- 46. may use two diif er to have cut in diff places.
- 48. add plamid to bacteria
- 49. end up w thousands of bac tea w diff sect of genome.
- 50. Southern and Northern Hybridization Analysis
- 51. Autoradiography