The document discusses various biotechniques and biotechnologies including:
- Recombinant DNA technology which is used for cloning and expressing genes as well as creating genomic and cDNA libraries.
- Blotting techniques like Southern, Northern, and Western blotting which separate DNA, RNA, and proteins via electrophoresis.
- Gene transformation techniques including direct methods like electroporation and indirect methods using Agrobacterium.
- DNA sequencing methods such as chemical, enzymatic, and automated approaches.
- Other techniques involving artificial chromosomes, exome sequencing, probing, fingerprinting, and CRISPR/Cas9.
Recombinant DNA Technology and Drug DiscoveryAshok Jangra
In these slide I discuss about the various DNA Technology and their biological importance and modern uses of these technologies. Here I also discussed about the oligonucleotide and new pharmaceutical approaches.
Recombinant DNA Technology and Drug DiscoveryAshok Jangra
In these slide I discuss about the various DNA Technology and their biological importance and modern uses of these technologies. Here I also discussed about the oligonucleotide and new pharmaceutical approaches.
Transfection methods (DNA to host cell) Erin Davis
Transfection of DNA to host cell can be done by various methods in lab scale.Gene gun,electroporation,lipofection .These methods are used to transfer DNA to the host cell.
RNA-Seq Analysis of Blueberry Fruit Development and RipeningAnn Loraine
Presentation at Plant Animal Genome Conference 2016 - features Integrated Genome Browser and developmental time course RNA-Seq data set from Illumina sequencing
Fungal Transformation in yeast and filamentous fungi
Introduction to Fungi
Background of fungal transformation
Transformation protocol
Transformation vectors
Integration into chromosomes
Biological applications of fungi
Conclusion
References
Transfection methods (DNA to host cell) Erin Davis
Transfection of DNA to host cell can be done by various methods in lab scale.Gene gun,electroporation,lipofection .These methods are used to transfer DNA to the host cell.
RNA-Seq Analysis of Blueberry Fruit Development and RipeningAnn Loraine
Presentation at Plant Animal Genome Conference 2016 - features Integrated Genome Browser and developmental time course RNA-Seq data set from Illumina sequencing
Fungal Transformation in yeast and filamentous fungi
Introduction to Fungi
Background of fungal transformation
Transformation protocol
Transformation vectors
Integration into chromosomes
Biological applications of fungi
Conclusion
References
In a detail description of the two major blotting techniques. Right from its history to the result interpretation put forth in a concise way. Helps understand these protocols with ease.
Nucleic acid techniques in diagnostic microbiologymohit kumar
in this presentation, you learn about the microbiological techniques which help in the molecular diagnosis of any single pathogens. with this, you aware of some commercially available kits for polymerase chain reaction both for realtime as well as conventional PCR and genome extraction kits.
PRINCIPLES OF PLANT BIOTECHNOLOGY
Subham Mandal ( Student )
B.Sc Horticulture , 2nd year
Uttar Banga Krishi Viswavidyalaya
Disclaimer : I am also a student so.. read it at your own risk
SUMMARY :
- Gene Transfer:
1. Agrobacterium-mediated transformation
2. Biolistic or particle bombardment
3. Electroporation
4. Microinjection
5. Protoplast fusion
- Procedure of Gene Cloning:
1. Isolation of DNA
2. Preparation of vector
3. Insertion of DNA
4. Transformation
5. Identification/screening
- PCR:
1. Denaturation
2. Annealing
3. Extension
- DNA fingerprinting:
1. DNA extraction
2. DNA fragmentation
3. Gel electrophoresis
4. Southern blotting
5. Hybridization
6. Detection
7. Analysis
- Transgenic:
1. Bt Cotton
2. Bt Brinjal
3. Golden Rice
4. Bt Rice
5. GM Mustard
- Molecular markers:
1. RFLP
2. AFLP
3. SSR
4. SNP
5. Indels
- Vectors:
1. Plasmid vectors
2. Cosmid vectors
3. Bacterial artificial chromosome (BAC) vector
- MAS (Marker-Assisted Selection):
1. Improvement of yield and quality
2. Enhancement of nutritional content
3. Development of stress-tolerant crops
4. Identification of disease-resistant plants
5. Improvement of crop traits through genetic modification
Anther Culture: Culturing immature pollen grains to produce haploid plantlets for breeding and genetic research.
Embryo Culture: Growing and developing plant embryos in vitro for clonal propagation and study of embryogenesis.
Pollen Culture: Culturing mature pollen grains to produce haploid plantlets and create new cultivars.
Ovule Culture: Culturing ovules for haploid or doubled haploid plant production and hybridization.
Somatic Embryogenesis: Inducing embryonic structures from somatic cells for clonal propagation and genetic modification.
Meristem Culture: Culturing the apical meristem for virus-free stock recovery and micropropagation.
Microarray -types, DNA chip, Principle and application of microarray, Preparation of DNA Chip, Affymetrix chip, microarray in genomics and proteomics, advantages and limitations of microarray
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
5. 1. Recombinant DNA Technology
- DNA Cloning
- Expression of Gene or DNA
- Reverse Transcription
- Genomic Libraries
- cDNA Libraries
- Transformation Experiments
- Molecular Markers
6. A. DNA Cloning or Gene Expression
- By using RDT, The desired segment of DNA or gene is being cloned
& expressed for human welfare…
7. B. Genomic Libraries & cDNA Libraries
-The RDT is being used to produce Genomic & cDNA Libraries which aims at
storage and preservation elite genes & germplasm of plant & animal species…
8. C. Molecular Markers
- Molecular Markers are the specific base sequences present on a DNA which
are referred to locate an another base sequence on that DNA…
9. D. Reverse Transcription
- The Reverse Transcription is the process in which the DNA is prepared by
using RNA as a template with the help of Reverse Transcriptase…
11. A. Southern Blotting Technique
- Southern Blotting is the technique used for separation & detection of DNA
bands over a gel on the basis of molecular weight & charge present on them…
12. B. Northern Blotting Technique
- Northern Blotting is the technique used for separation & detection of RNA
bands over a gel on the basis of molecular weight & charge present on them…
13. C. Western Blotting Technique
- Western Blotting is the technique used for separation & detection of protein
molecules over a gel on the basis of molecular weight & charge present on them…
14. 3. Gene Transformation Techniques
A. Indirect Gene Transformation
- Agrobacterium mediated gene Transformation
- Agro-infection
B. Direct Gene Transformation
- Electroporation
- Chemoporation
- Microinjection
- Lipofection
- Pollen Transformation
- Particle Gun Method
- Fibre Mediated DNA delivery
- Laser Induced DNA delivery
15. A. Indirect Gene Transformation
- In this method, a gene is transformed into host by using an intermediate
agent like agrobacterium…
16. B. Direct Gene Transformation
- In this method, a gene is transformed into host without using any intermediate
agent but induced by physical or chemical materials…
17. 4. DNA Sequencing
- Chemical Method / Maxam & Gilbert Method
- Enzymatic Method / Sanger Coulson Method
- Pyrosequencing
- Automated DNA Sequencing
- Primer Walking
- RNA Sequencing
18. A. Chemical Method of DNA Sequencing
- In this method, The DNA is sequenced by using some chemicals i.e. chemically
prepared bases…
19. B. Enzymatic Method of DNA Sequencing
- In this method, The DNA is sequenced by using some enzymes like restriction
enzymes, polymerases, etc…
