This document discusses biosafety level 3 (BSL-3) laboratories and containment procedures. BSL-3 involves serious or potentially lethal diseases that can be transmitted via inhalation. Key requirements for BSL-3 include controlled access, medical surveillance of personnel, HEPA filtration of air, protective equipment and clothing for personnel, decontamination of waste, and specific lab design features like an anteroom, gas-tight walls and doors, and inward airflow. The pathogens that require BSL-3 containment include tuberculosis, influenza, SARS, rabies, and plague. Strict safety practices and government oversight are necessary to work safely with these dangerous microbes.

1. BIOSAFETY LEVELS (BSL-3)
FOR
SPECIFIC MICROORGANISMS
PRESENTATION BY:
SOURISH DAS
BSC , 6TH SEMESTER

2. Contents:
• What is Biosafety Level?
• Principles of Biosafety.
• Standard Laboratory Practices.
• Types of BSL according to Risk Assessment.
• Specific Controls.
• Basic Precautions.
• BSL-3
• HEPA Filter.
• Safety Practices.
• Laboratory Design.
• Specific Organisms.
• Conclusion.

3. What is a Biosafety Level ?
A biosafety level (BSL), or
pathogen/protection level, is a set of
biocontainment precautions required to
isolate dangerous biological agents in an
enclosed laboratory facility.

4. Principles of Biosafety:
• Containment.
• Risk Assessment.

5. Standard Laboratory Practices :
Microbiology Laboratory Levels:

6. Types Of Biosafety Levels:
Risk Group Individual Risk Community Risk
1 No, low No, low
2 Moderate Low
3 High Low
4 High High

7. Specific controls based
on:
• Infectivity of the disease.
• Severity of the disease.
• Source of the agent.
• Route of invasion.

8. Basic Precautions :-
• Designing of the facility.
• Safety environment.
• Laboratory practices.

9. BIOSAFETY LEVEL 3 (BSL-3):
Cause serious or
fatal diseases by
Inhalation.

10. What is Biosafety Level 3 ?
• Biosafety Level 3 is applicable to clinical,
diagnostic, teaching, research, or production
facilities in which work is done with
indigenous or exotic agents which may cause
serious or potentially lethal disease as a
result of exposure by the inhalation route.

11. Biosafety Level 3 :
Controlled By Government Agencies -
In India, apex bodies like Indian Council of Medical Research (ICMR),
Council of Scientific and Industrial Research (CSIR), Department of
Biotechnology (DBT), Indian Council of Agricultural Research (ICAR),
and Department of Science and Technology (DST) are playing
important role in biomedical research and public health.
Medical Surveillance -
The BSL-3 program is for individuals who are conducting research
with pathogens and toxins that pose potential threats to human,
plant, and animal life and their personal health will be evaluate by
the Occupational Health Professional (OHP).

12. o What is HEPA filter?
• A HEPA filter is designed to target very small particles, and therefore doesn’t
work like a typical membrane filter, where particles larger than a given pore size
of a filter are captured. Instead, HEPA filters rely on a combination of three
mechanisms to trap particles.
• The first mechanism is interception, where particles being carried in the airflow
around the filter fibers adhere to the filter.
• Particles must be within one radius of the filter fiber to be captured. Larger
particles are often captured by the second mechanism, impaction.
• The final mechanism is diffusion that occurs because of the way microscopic
particles move and interact with surrounding molecules. This is described as
Brownian motion, where molecules move in a random, zig-zag pattern because
they collide with surrounding molecules.
• A HEPA filter does not remove particles only above a certain size. It can
effectively remove particles both above and below 0.3 microns. In general, large
particles (greater than 0.3 microns in diameter) are captured by both the
impaction and interception mechanisms, whereas small particles (less than 0.1
micron in diameter) are captured by the diffusion mechanism.
• The most penetrating particle size is 0.3 microns.

13. BSL - 3 FACILITY :

14.  Basic Safety Practices :
• Hand washing.
• Wearing lab coat, glove and eye protection.
• Limited access to people.
• No mouth pipetting.
• Cleaning and decontamination of area.
• Warning signs.
+
Using of Biosafety cabinet.
Trained personnel.
Availability of eye wash station.
Vaccination for the workers (if applicable).

15. Additional Laboratory Practices :
* Periodic medical testing for workers.
* Full body garment with respiratory
protection.
* Restricted access at all time.

16. Basic Laboratory Design for Biosafety
Level 3 Laboratories:
 GENERAL:
• The laboratory must consist of an anteroom and laboratory rooms.
• The facility must have gas-impermeable walls, ceilings, and floors. Air gaps
under doors are acceptable for directional airflow. If the door gaps are sealed,
the laboratory suite must not leak gaseous decontamination materials.
• Air balance must be set so air from low hazard rooms flows into rooms with
higher hazards, and entry into the laboratory requires passage through two
doors.
• Consideration should be given to providing a separate equipment room (to
isolate heat load and high hazard equipment like centrifuges) within the
laboratory.
• Biosafety level 3 cabinets are used in this level of biosafety.

17. WARNING SIGNS BSL 3 LAB PERSONNEL

18. BSL 3 LABORATORY

19. Specific Organisms :
The pathogens that require BSL-3
laboratories include HIV, H1N1
influenza,H5N1 avian influenza,
Yersinia pestis, Mycobacterium
tuberculosis, SARS (severe acute
respiratory syndrome), Rabies Virus, West
Nile Virus, Ricketts, etc.

20. Conclusion:
•Biosafety is used to protect us from
harmful incidents. Monitoring of the
biosafety standards must be met by labs
in order to prevent the accidental
release of potentially destructive
biological material.

21. Thank you.