Ethically-donated fresh, functional human tissues can be used to better predict the likely effect of drugs during clinical trials. As the closest possible model of drug function in patients, fresh human tissues are playing an increasingly important role in de-risking the drug discovery process, helping pharmaceutical and biotechnology companies to make earlier go/no-go decisions based on human data.
GVK BIO vivarium is AAALAC certified and registered with CPCSEA (Committee for the Purpose of Control and Supervision of Experiments on Animals), Government of India.
ARTIFICIAL ORGANS.
We discussed a Brief History and Introduction of Artificial Organs.
We also discussed the Various Manufacturing Process and Application of Artificial Organs and finally we discussed the Pros and Cons of Artificial Organs.
GVK BIO vivarium is AAALAC certified and registered with CPCSEA (Committee for the Purpose of Control and Supervision of Experiments on Animals), Government of India.
ARTIFICIAL ORGANS.
We discussed a Brief History and Introduction of Artificial Organs.
We also discussed the Various Manufacturing Process and Application of Artificial Organs and finally we discussed the Pros and Cons of Artificial Organs.
Homework X - Biomaterials Science.
An extracorporeal artificial organ is a man-made device that is integrated into a human — interfacing with living tissue — to replace a natural organ, for the purpose of duplicating or augmenting a specific function or a group of related functions so the patient may return to a normal life as soon as possible. The replaced function doesn't necessarily have to be related to life support, but it often is.
IST - 4th Year - 2nd Semester - Biomedical Engineering.
Chemical biology approaches for predicting human drug and chemical safety. Presentation for Thompson Hall Science and Mathematics Seminar Series at the University of Puget Sound, February 12, 2015, Tacoma, WA. Ellen Berg.
Stem cells for artificial organ regenerationElvis Samuel
A stem cell is a cell with the unique ability to develop into specialized cell types in the body. This presentation details the regeneration of artificial organs using stem cells
When your focus is small molecules, biomarkers, or protein biotherapeutics,
QPS’ LC-MS/MS laboratories provide a full range of
bioanalytical solutions to support drug development
from discovery through clinical development.
Haematological and Serum Biochemical Parameters of Mature Harco Cocks Treated...IJEAB
Twenty sexually matured (24 weeks old) healthy Harco cocks were used to determine the effect of Gonadotrophin (Diclair®) on haematology and serum biochemistry. The cocks were divided into 4 treatment groups of 5 cocks per group identified as T1 (control) administered with 1ml physiological saline, T2, administered with 6.75i.u Diclair® and T4, administered with 20.25i.u Diclair®, with one cock per replicate in a completely Randomized Design (CRD). The injections were dividedinto three doses each and administered intramuscularly in the thigh for three consecutive days. One week after Diclair® treatments, five birds from each group were bled from the wing veins for haematology and serum biochemistry. Results of this study showed significant differences (P<0.05)>0.05) among the treatment groups. Basophils were not detected among the treatment groups. The results further showed significant differences (P<0.05)>0.05) among the treatment groups. However, the values were within the normal ranges, indicating that Diclair® had no deleterious effect on these parameters.
In-vitro Cell culture is developing rapidly with the primary goal being to model human physiology in a realistic way that predicts clinical results. This presentation shares the strategy being employed by Kirkstall Ltd to provide a practical solution to the challenges of creating an organ on-a-chip.
A presentation on Tissue Engineering made by Deepak Rajput. It was presented as a seminar requirement at the University of Tennessee Space Institute in Spring 2009.
Homework X - Biomaterials Science.
An extracorporeal artificial organ is a man-made device that is integrated into a human — interfacing with living tissue — to replace a natural organ, for the purpose of duplicating or augmenting a specific function or a group of related functions so the patient may return to a normal life as soon as possible. The replaced function doesn't necessarily have to be related to life support, but it often is.
IST - 4th Year - 2nd Semester - Biomedical Engineering.
Chemical biology approaches for predicting human drug and chemical safety. Presentation for Thompson Hall Science and Mathematics Seminar Series at the University of Puget Sound, February 12, 2015, Tacoma, WA. Ellen Berg.
Stem cells for artificial organ regenerationElvis Samuel
A stem cell is a cell with the unique ability to develop into specialized cell types in the body. This presentation details the regeneration of artificial organs using stem cells
When your focus is small molecules, biomarkers, or protein biotherapeutics,
QPS’ LC-MS/MS laboratories provide a full range of
bioanalytical solutions to support drug development
from discovery through clinical development.
Haematological and Serum Biochemical Parameters of Mature Harco Cocks Treated...IJEAB
Twenty sexually matured (24 weeks old) healthy Harco cocks were used to determine the effect of Gonadotrophin (Diclair®) on haematology and serum biochemistry. The cocks were divided into 4 treatment groups of 5 cocks per group identified as T1 (control) administered with 1ml physiological saline, T2, administered with 6.75i.u Diclair® and T4, administered with 20.25i.u Diclair®, with one cock per replicate in a completely Randomized Design (CRD). The injections were dividedinto three doses each and administered intramuscularly in the thigh for three consecutive days. One week after Diclair® treatments, five birds from each group were bled from the wing veins for haematology and serum biochemistry. Results of this study showed significant differences (P<0.05)>0.05) among the treatment groups. Basophils were not detected among the treatment groups. The results further showed significant differences (P<0.05)>0.05) among the treatment groups. However, the values were within the normal ranges, indicating that Diclair® had no deleterious effect on these parameters.
In-vitro Cell culture is developing rapidly with the primary goal being to model human physiology in a realistic way that predicts clinical results. This presentation shares the strategy being employed by Kirkstall Ltd to provide a practical solution to the challenges of creating an organ on-a-chip.
A presentation on Tissue Engineering made by Deepak Rajput. It was presented as a seminar requirement at the University of Tennessee Space Institute in Spring 2009.
B2 B Marketing Excellence Asset Brand OverviewDana Theus
The B2B Marketing Excellence Asset BrandSM Program supports companies that are committed to leveraging brand through strategic shifts in their corporate strategy.
Integratori alimentari senza glutine al gluten free expo 2012GJAV
Con l'occasione della prima edizione del Gluten Free Expo di Brescia viene presentata la linea innovativa di integratori per sportivi a marchio GJAV.
Il marchio GJAV ha come obiettivo il miglioramento della performance atletica, fisica e mentale degli sportivi. GJAV è la prima linea italiana di integratori alimentari per sportivi, certificati senza glutine, senza lattosio, creulty free, doping tested, con integratori formulati anche per gli atleti agonisti vegani, vegetariani e celiaci.
GJAV si caratterizza per un approccio salutistico verso l'utilizzo degli integratori alimentari per sportivi con una particolare attenzione all'assenza di allergeni ma anche di conservanti, coloranti e di tutti quei coadiuvanti tecnologici che non migliorano il valore nutrizionale di un prodotto.
GJAV è un progetto di eccellenza che ha coinvolto Enti, Istituzioni, Associazioni, supportato da una visione innovativa del prodotto. La linea è stata presentata al SANA 2012 ha partecipato al concorso SANA Novità ed ha un premio ed una menzione speciale per “Prodotto innovativo con pack ad Impatto Zero”.
Gli integratori alimentari GJAV sono inseriti nel prontuario dell'Associazione Italiana Celiachia (AIC) e sono indicati per atleti celiaci e per quelli che soffrono di sensibilità al glutine. Ogni lotto di produzione viene certificato e titolato senza glutine mediante test ELISA R5. I risultati di tutte le analisi sono disponibili sul sito.
Grazie alla metodologia produttiva prima della commercializzazione ogni lotto viene rigorosamente testato presso il laboratorio Regionale Antidoping, accreditato del CONI (WADA) per escludere le contaminazione con sostanze dopanti. I risultati di tutte le analisi sono disponibili sul sito.
Le formulazioni in polvere o compresse, sono studiate per fornire un rapido assorbimento dei principi attivi e per favorire il recupero ed il benessere di ogni atleta sottoposto a sforzo intenso e prolungato. I prodotti sono formulati con sali minerali alcalinizzanti pH alcalin ®, con aminoacidi essenziali e con principi attivi purissimi, di sintesi, minerale e vegetale.
GJAV ha un anima verde! I prodotti sono certificati ad Impatto Zero ® da Life Gate. Le emissioni di CO2, derivante dalla produzione della plastica dei barattoli, dalle etichette e dalla movimentazione logistica dei prodotti viene compensata mediante crediti (RECS) generati da interventi di creazione e tutela di foreste in Amazzonia.
Galerie des Beaux Arts. Een unieke galerie waar u in een stijlvolle omgeving geniet van een bijzondere collectie oude en hedendaagse kunstobjecten. Moderne schilderijen en beeldhouwwerken, antieke meubelen en klokken, kostelijke sieraden en juwelen, verfijnde kleinoden in zilver en goud …
Beeld en Geluid als grootste nationale beheerder van audiovisueel erfgoed.
Het beschikbaarstellen van av-bronnen, het (zelf) creëren van context hierbij en het faciliteren van het zélf arrangeren en creëren met av-bronnen. Les 2.0 , ED*IT en Academia als rijke bron voor modern onderwijs. De Beeld en Geluid experience als etalage van het archief en als plaats voor informeel leren.
Assessing gastrointestinal toxicity using human tissues bioptaBiopta Inc.
Many drugs adversely affect the gastrointestinal system. This presentation describes the use of ethically-donated human tissue samples in the prediction of drug-induced gastrointestinal toxicity and will be of interest to scientists involved in drug discovery and development.
Human Cell Systems Biology for Drug Discovery and Chemical Safety. Presentation at the 7th Brazilian Symposium on Medicinal Chemistry, November 12, 2014, Campos do Jordao-SP, Brazil. Ellen Berg.
A Comprehensive How-To Demonstration of Higher Throughput Excitation-Contract...InsideScientific
From myocyte isolation to data acquisition, analysis, and post-analysis plotting, join Dr. Michiel Helmes and Dr. Diederik Kuster as they demonstrate best practices and new techniques in high-content, higher throughput investigations of excitation-contraction coupling in isolated cardiomyocytes.
During this 60 minute live webinar, Michiel Helmes and Diederik Kuster will deliver a comprehensive how-to demonstration of higher throughput excitation-contraction coupling investigations with isolated cardiomyocytes.
Characterizing excitation-contraction coupling in isolated cardiac myocytes has been essential to our understanding of heart function. Historically these studies have been constrained by lower throughput data collection and limited sample sizes. Because isolated myocytes display a high degree of functional variability, acquiring data from more myocytes is required for greater accuracy and statistical confidence.
In this webinar, we will demonstrate important aspects of data collection from myocyte isolation to precision data acquisition, data analysis, and post-analysis interpretation. We will focus on how to get the most out of every isolation, how to collect quality data consistently, why statistical power matters, and how to get statistically meaningful data in hours.
Key Topics Include:
Get more from less: learn to maximize data from each animal
Quality and quantity: best practices for data acquisition
Knowledge is power: understand what your data means and how to interpret it
Go beyond numbers: see how to get automated, same-day post-analysis data plotting and processing
An organ-on-a-chip (OOC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems, a type of artificial organ
Specialty pathology endpoints for all clients, including complex immunohistochemistry, in situ hybridization, image analysis, artificial intelligence, stereology, tissue cross-reactivity, and electron microscopy.
Organs-on-chips (OoCs) are systems containing engineered or natural miniature tissues grown inside microfluidic chips. To better mimic human physiology, the chips are designed to control cell microenvironments and maintain tissue-specific functions. Combining advances in tissue engineering and microfabrication, OoCs have gained interest as a next-generation experimental platform to investigate human pathophysiology and the effect of therapeutics in the body. There are as many examples of OoCs as there are applications, making it difficult for new researchers to understand what makes one OoC more suited to an application than another.
What process is used to obtain cells from bone marrow and normal peripheral blood?
What is the best cell counting and viability method for primary cells?
AllCells, your primary cells research partner, and Nexcelom Bioscience, your cell counting experts, have joined together in an exclusive collaboration to host a free webinar to help educate researchers and present data from their own experiences.
These presentation includes the information about the replacement of animal experiments (invivo tests) with all the alternative methods like invitro tests and in-silico methods which are used in present century and made the research work easy for pre-clinical and clinical trials.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
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Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
The prevalence of testicular torsion in adult patients hospitalized with acute scrotal pain is approximately 25 to 50 percent
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Pharynx and Clinical Correlations BY Dr.Rabia Inam Gandapore.pptx
Biopta company presentation
1. The Use of Fresh Human Tissues to
Predict Drug Efficacy, Safety and
Absorption
2. Human Tissues Predict
Clinical Safety and Efficacy
• Functional data produced in ex vivo human tissue
• Provide information to pharmaceutical and biotechnology companies
about the behaviour of their compounds in human relevant systems
• Efficacy
• Do compounds work in human tissue
• Absorption
• Are compounds permeable through human gut, skin, bronchi
• Safety Pharmacology
• Do compounds show adverse effects in human tissue – e.g. human coronary arteries
• Comparative studies with other species strengthens this capability
3. Biopta- Leaders in Fresh
Human Tissue Research
Beltsville,
Maryland
Glasgow, UK• HQ and lab in Glasgow, UK
• Lab facility in Maryland
• Founded 2002
• First, and currently only, GLP
compliant functional human
tissue CRO
• Experts in human tissue
research and procurement
• 50% of scientists at PhD level
• Pharma and Biotech
customers worldwide
• Tailored human functional
studies with responsive and
flexible collaboration to achieve
clients’ goals
4. Biopta’s Tissue Network: Fresh Human
Tissues Sourced 24/7 in USA and UK
Surgical residual tissues
Nontransplantable
organs
• Biopta receives ethically donated fresh tissues via two
main routes:
- Surgical residual tissues (mainly UK)
-Non-transplantable organs (mainly USA)
• Biopta’s facilities are open 24/7 to receive the tissues,
dissect and conduct experiments – Laboratories ( )
placed strategically to minimise transport time
• Biopta is an ethically approved Research Tissue Bank
5. Biopta’s Scientific Expertise: Techniques
to Assess Human Tissue Pharmacology
• Biopta has a track record of producing high quality decision-making
data from human and animal tissue in a variety of functional assay
systems
• Experience with addressing complex issues across a broad range
of therapeutic areas and mechanistic pathways
• Staffed with highly trained laboratory scientists skilled in tissue
dissection and experimentation, and with experienced study
directors with a track record of coordinating complex programs of
work
6. Biopta – How we work
Fresh Human
Tissue
Pharmacology
Techniques
Scientific
Experience
Scientific
Question
BioptaClient
Protocol design
e.g. Blood pressure change e.g. Resistance arteries e.g. Tissue baths
-10 -9 -8 -7 -6 -5
0
25
50
75
100
log M [acetylcholine]
%Relaxation
-9 -8 -7 -6 -5 -4
0
25
50
75
100
log M [phenylephrine]
%Constriction
Decision-making data
Relaxation to
Acetylcholine
Constriction to
Phenylephrine
Raw data traces Analysed data Interim and final reports
7. Tissue baths Ussing
Chambers
Wire myographs Perfusion
myographs
Ex vivo
cultures
Biopta Techniques
• Contractility and relaxation of tissues
• vascular and myocardial tissue
• lung tissue
• gastrointestinal tissue
• genitourinary tissue
• Inflammation, cytokine and gene exp. analysis
• lung tissue
• gastrointestinal tissue
• Compound penetration and metabolism
• gastrointestinal tissue
• lung tissue
• skin tissue
• Ion channel activity
• lung tissue
• gastrointestinal tissue
8. Biopta Human Tissues
Blood Vessels: Gastrointestinal: Others:
- resistance arteries - large intestine - skin
- coronary arteries - small intestine - skeletal muscle
- renal arteries - oesophagus
- pulmonary arteries - stomach
- cerebral arteries
Cardiovascular: Genitourinary: Respiratory:
- atrial appendage - bladder - bronchus
- ventricular muscle - urethra - trachea
- uterus
From both healthy and diseased patients: heart failure, asthma, COPD, diabetes,
atherosclerotic tissue, ischaemic limbs, psoriatic and atopic dermatitis skin biopsies
Other tissues are available from Biopta’s wide tissue network. Contact us to check availability: info@biopta.com
9. Constriction
↑ blood pressure
Relaxation
↓ blood pressure
0 min 10 min 15 min 20 min 25 min5 min
Fresh human tissue
(heart shown here but many
tissue and organ types used)
Tissue transported
immediately to
laboratory
Scientist isolates
the tissues of
interest
The “living”
tissues are
exposed to
drugs
Small blood
vessels
isolated from
tissue sample
A Biopta project: patient > lab > translational data
10. Using human tissues de-risks development
by avoiding species differences
Sensitivity differences to the vasoconstrictor 5-HT
(serotonin) in subcutaneous resistance arteries of dogs
and humans
-10 -9 -8 -7 -6 -5 -4
0
25
50
75
100
Canine
Human
5-HT log [M]
%constriction
(ofKPSSresponse)
Where there are clear species differences between humans and animals, human
isolated tissues can avoid such differences
Relaxation CRC to PDE2 inhibitor in
canine and human subcutaneous
resistance arteries
-7.0 -6.5 -6.0 -5.5 -5.0 -4.5 -4.0
-10
15
40
65
90
**
**
** canine sc arteries
human sc arteries
Log M [PDE2 inhibitor]
Relaxationasa%of
300nMU46619preconstriction
Biopta collaboration with J&J demonstrating the involvement
of PDE2 in the control of subcutaneous artery tone
(results published at the Safety Pharmacology Society
Meeting, 2009)
11. Biopta’s data impacts across
the whole drug development process
Clinical trials
Discovery phase Development phase
Clinical trials
Phase 1 II III IV
Lead
optimisation
Lead
identification
Hit
identification
Target
validation
Nomination
phase
Target
validation
Compound
characterisation DMPK Safety Clinical observations
12. Why Biopta?
• Add commercial value and reduce risk of clinical failure
• Efficacy and safety data generated in target species eliminating species
differences
• Conduct experiments to directly compare animal and human data
• Biopta is the world leading GLP-compliant laboratory for testing in fresh,
functional human tissues
Email: KeithBowers@biopta.com
Tel: +44 141 330 3831
www.biopta.com