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GVK BIO vivarium is AAALAC certified and registered with CPCSEA (Committee for the Purpose of Control and Supervision of Experiments on Animals), Government of India.
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Biopta company presentation
Ethically-donated fresh, functional human tissues can be used to better predict the likely effect of drugs during clinical trials. As the closest possible model of drug function in patients, fresh human tissues are playing an increasingly important role in de-risking the drug discovery process, helping pharmaceutical and biotechnology companies to make earlier go/no-go decisions based on human data.
my synopsis. Leslie for printing
This thesis investigated the effect of cadmium sulfate exposure and treatment with the mushroom Pleurotus florida on albino rats. Rats were divided into groups that received various doses of cadmium and mushroom extract. Organs and blood were analyzed after 4, 8, 12, and 16 days. Histological analysis found damage to heart, liver, and kidneys in cadmium-exposed rats, which was reduced by mushroom treatment. Behavioral changes and clinical signs of toxicity were also observed with cadmium exposure. The mushroom extract showed protective effects on the organs. In conclusion, Pleurotus florida has potential for reducing cadmium-induced organ damage.
effect of cyclophosphamide on testis of rat
1. The study investigated the effects of cyclophosphamide (CP), an anticancer drug, on the testes of male rats (Rattus rattus).
2. CP was found to cause adverse effects on the morphology and histology of the rat testes including a reduced number of spermatozoa in the lumen and disorganized spermatogenic cells with fewer spermatids.
3. The results suggest that CP can cause histoarchitectural changes in the rat testes that may impair fertility, demonstrating the reproductive toxicity of this drug even at low doses over short periods of time.
Toxicology of genetically modified sheep meat
This study evaluated the safety of meat from genetically modified sheep overexpressing TLR4 compared to meat from wild-type sheep in a 90-day feeding study using Sprague-Dawley rats. Meat samples from GM and wild-type sheep were analyzed for composition. Rats were fed diets supplemented with 3.75%, 7.5%, or 15% GM or wild-type sheep meat powder. Results showed no differences in body weight, blood parameters, organ weights, or histopathology between rats fed GM versus wild-type meat. The study concluded that meat from GM sheep overexpressing TLR4 showed no adverse or toxic effects compared to wild-type sheep meat.
EFFECT OF CYCLOPHOSPHAMIDE(anticancer drug) ON TESTIS
This document describes a study that examined the histological effects of the anticancer drug cyclophosphamide (CPA) on the testes of male rats (Rattus rattus). Adult male rats were injected with CPA at 40mg/kg body weight alternately for 15 days. Histological analysis of the testes found several changes compared to controls, including a reduced number of spermatozoa in the lumen, disorganized spermatogenic cells with fewer spermatids, and prominent Leydig cells. The lumens also contained relatively fewer spermatozoa. The study suggests CPA can induce histological changes in the testes that may impact fertility.
Efficient recombination by tamoxifen inducible cre
This study sought to develop a tamoxifen-inducible form of Cre recombinase (Cre-ERTM) that would allow for temporal control of gene activation and inactivation in mice. The researchers generated a Cre-ERTM transgenic line and demonstrated that it produced recombination in a dose-dependent and tissue-dependent manner following tamoxifen administration. Recombination occurred rapidly within tissues and cell culture following tamoxifen treatment and nuclear localization of Cre-ERTM. This tool provides a method for temporally regulated genetic modification in mice.
EFFECTS OF LAYING KADHAKNATH HEN SERUM AND ANTI-PROLACTIN MEDICATION [BROMOCR...
EFFECTS OF LAYING KADHAKNATH HEN SERUM AND ANTI-PROLACTIN MEDICATION [BROMOCR...International Research Journal of Modernization in Engineering Technology and Science
This research is carried out in order to improve the production of eggs in indigenous chicken by reducing the
inter-sequence stopped days through use of anti-prolactin agent (Bromocriptine) and serum from laying hen.
Sixty-four indigenous (deshi) chickens of 20-22 weeks of age, were randomly assigned into four groups (i, j, k
and l) and each group consisting of 16 hens. Control was designated as Group I and Bromocriptine orally at a
dose of 641μg/bird/day was used to treat group j, group k was treated with serum of laying kadhaknath hen
serum at a dose of 1 ml intramuscularly/bird/day and group l was treated with both Kadhaknath serum and
Bromocriptine at doses given to group j and k for the period of 15 March, 2019 to 16 June, 2019 and egg
production, stopped days, prolactin level, hematological parameter and egg qualities were observed. A
significant increase (p<0.05) in Egg production was noticed in all treated groups in comparison to the groups
which were in non- treated control and group k showed the highest production. All treatment groups depicted a
significant decrease (p<0.05) in stopped days and prolactin levels and lowest were observed in hens of group l.
In hematological values between the chicken group, no significant differences were noticed. The present study
reveals that combined treatment with Bromocriptine and serum from laying kadhaknath hen increases egg
production without affecting the health of indigenous chickens.Artificial Organs
This presentation covers different artificial organs and techniques used to develop stem cell-based organs
Recommended
Biopta company presentation
Ethically-donated fresh, functional human tissues can be used to better predict the likely effect of drugs during clinical trials. As the closest possible model of drug function in patients, fresh human tissues are playing an increasingly important role in de-risking the drug discovery process, helping pharmaceutical and biotechnology companies to make earlier go/no-go decisions based on human data.
my synopsis. Leslie for printing
This thesis investigated the effect of cadmium sulfate exposure and treatment with the mushroom Pleurotus florida on albino rats. Rats were divided into groups that received various doses of cadmium and mushroom extract. Organs and blood were analyzed after 4, 8, 12, and 16 days. Histological analysis found damage to heart, liver, and kidneys in cadmium-exposed rats, which was reduced by mushroom treatment. Behavioral changes and clinical signs of toxicity were also observed with cadmium exposure. The mushroom extract showed protective effects on the organs. In conclusion, Pleurotus florida has potential for reducing cadmium-induced organ damage.
effect of cyclophosphamide on testis of rat
1. The study investigated the effects of cyclophosphamide (CP), an anticancer drug, on the testes of male rats (Rattus rattus).
2. CP was found to cause adverse effects on the morphology and histology of the rat testes including a reduced number of spermatozoa in the lumen and disorganized spermatogenic cells with fewer spermatids.
3. The results suggest that CP can cause histoarchitectural changes in the rat testes that may impair fertility, demonstrating the reproductive toxicity of this drug even at low doses over short periods of time.
Toxicology of genetically modified sheep meat
This study evaluated the safety of meat from genetically modified sheep overexpressing TLR4 compared to meat from wild-type sheep in a 90-day feeding study using Sprague-Dawley rats. Meat samples from GM and wild-type sheep were analyzed for composition. Rats were fed diets supplemented with 3.75%, 7.5%, or 15% GM or wild-type sheep meat powder. Results showed no differences in body weight, blood parameters, organ weights, or histopathology between rats fed GM versus wild-type meat. The study concluded that meat from GM sheep overexpressing TLR4 showed no adverse or toxic effects compared to wild-type sheep meat.
EFFECT OF CYCLOPHOSPHAMIDE(anticancer drug) ON TESTIS
This document describes a study that examined the histological effects of the anticancer drug cyclophosphamide (CPA) on the testes of male rats (Rattus rattus). Adult male rats were injected with CPA at 40mg/kg body weight alternately for 15 days. Histological analysis of the testes found several changes compared to controls, including a reduced number of spermatozoa in the lumen, disorganized spermatogenic cells with fewer spermatids, and prominent Leydig cells. The lumens also contained relatively fewer spermatozoa. The study suggests CPA can induce histological changes in the testes that may impact fertility.
Efficient recombination by tamoxifen inducible cre
This study sought to develop a tamoxifen-inducible form of Cre recombinase (Cre-ERTM) that would allow for temporal control of gene activation and inactivation in mice. The researchers generated a Cre-ERTM transgenic line and demonstrated that it produced recombination in a dose-dependent and tissue-dependent manner following tamoxifen administration. Recombination occurred rapidly within tissues and cell culture following tamoxifen treatment and nuclear localization of Cre-ERTM. This tool provides a method for temporally regulated genetic modification in mice.
EFFECTS OF LAYING KADHAKNATH HEN SERUM AND ANTI-PROLACTIN MEDICATION [BROMOCR...
EFFECTS OF LAYING KADHAKNATH HEN SERUM AND ANTI-PROLACTIN MEDICATION [BROMOCR...International Research Journal of Modernization in Engineering Technology and Science
This research is carried out in order to improve the production of eggs in indigenous chicken by reducing the
inter-sequence stopped days through use of anti-prolactin agent (Bromocriptine) and serum from laying hen.
Sixty-four indigenous (deshi) chickens of 20-22 weeks of age, were randomly assigned into four groups (i, j, k
and l) and each group consisting of 16 hens. Control was designated as Group I and Bromocriptine orally at a
dose of 641μg/bird/day was used to treat group j, group k was treated with serum of laying kadhaknath hen
serum at a dose of 1 ml intramuscularly/bird/day and group l was treated with both Kadhaknath serum and
Bromocriptine at doses given to group j and k for the period of 15 March, 2019 to 16 June, 2019 and egg
production, stopped days, prolactin level, hematological parameter and egg qualities were observed. A
significant increase (p<0.05) in Egg production was noticed in all treated groups in comparison to the groups
which were in non- treated control and group k showed the highest production. All treatment groups depicted a
significant decrease (p<0.05) in stopped days and prolactin levels and lowest were observed in hens of group l.
In hematological values between the chicken group, no significant differences were noticed. The present study
reveals that combined treatment with Bromocriptine and serum from laying kadhaknath hen increases egg
production without affecting the health of indigenous chickens.Artificial Organs
This presentation covers different artificial organs and techniques used to develop stem cell-based organs
Introduction to alternatives to animal testing in toxicology
Introduction to alternatives to animal testing in toxicologyCenter for Alternatives to Animal Testing
This document provides an introduction to general toxicology using the zebrafish egg model. It discusses the author's background working with alternative test methods and regulatory affairs. The principles of toxicology are outlined, including common in vivo models and alternative test methods. The zebrafish egg model is presented as a universal model for applications like acute toxicity testing, developmental toxicity assessment, safety pharmacology, and mammalian organotoxicity prediction. Protocols for zebrafish husbandry, egg production, development stages, and teratogenicity testing are described.6
1) Treatment with 50 nM of the histone deacetylase inhibitor trichostatin A (TSA) improved the developmental competence of interspecies somatic cell nuclear transfer (iSCNT) embryos reconstructed from cat somatic cells and bovine oocytes.
2) TSA treatment at 50 nM resulted in significantly higher rates of cleavage and blastocyst formation compared to untreated iSCNT embryos.
3) TSA treatment at 50 nM increased histone H3 lysine 9 (H3K9) acetylation levels in iSCNT embryos to levels similar to in vitro fertilized embryos, whereas untreated iSCNT embryos had lower acetylation levels.
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Twenty sexually matured (24 weeks old) healthy Harco cocks were used to determine the effect of Gonadotrophin (Diclair®) on haematology and serum biochemistry. The cocks were divided into 4 treatment groups of 5 cocks per group identified as T1 (control) administered with 1ml physiological saline, T2, administered with 6.75i.u Diclair® and T4, administered with 20.25i.u Diclair®, with one cock per replicate in a completely Randomized Design (CRD). The injections were dividedinto three doses each and administered intramuscularly in the thigh for three consecutive days. One week after Diclair® treatments, five birds from each group were bled from the wing veins for haematology and serum biochemistry. Results of this study showed significant differences (P<0.05)>0.05) among the treatment groups. Basophils were not detected among the treatment groups. The results further showed significant differences (P<0.05)>0.05) among the treatment groups. However, the values were within the normal ranges, indicating that Diclair® had no deleterious effect on these parameters.
Mice as an experimental animal
Mice are commonly used as experimental animals in research due to their genomic and physiological similarities to humans, low cost, short lifespan, and ability to be bred easily in a laboratory setting. There are several types of mice used, including inbred strains like C57BL/6 and BALB/c, with BALB/c being the most widely used strain. Mice are classified by age, from neonatal to aged adults. Transgenic and knockout mouse models also exist where genes are inserted or removed to study their functions. Mice are useful for studies in areas like toxicity, teratogenicity, genetics, cancer, and the screening of drugs.
Hammerman Xenotransplantation of organ primordia Curr Opin Org TX 2014
Organ primordia from pig embryos have been transplanted with some success to treat end-stage renal disease and diabetes in animal models. Embryonic kidney primordia transplanted into rats differentiated into functioning kidneys that attracted a blood supply from the host, though immune suppression was required. Embryonic pancreas primordia obtained very early in development engrafted long-term in diabetic rats and rhesus macaques without immune suppression, correcting glucose levels. Later attempts involved transplanting adult porcine islets into animals that had previously received embryonic pancreas transplants, to supplement insulin production.
Transgenic animals and process to make transgenic animals
The document summarizes topics related to transgenic animals and gene therapy. It discusses transgenic cows, sheep, poultry, and fish. For each animal, it describes the process used to create transgenic versions, including pronuclear microinjection and somatic cell nuclear transfer. Benefits include producing human therapeutic proteins and altering milk composition. Challenges include high costs and low success rates. Gene therapy techniques like viral vectors and electroporation are explained for inserting genes into tissues to treat disease. Somatic gene therapy aims to modify individual patients while germline gene therapy alters heritable genes passed to offspring.
Stem Cells in the Treatment of Osteoporosis
This study investigated using mesenchymal stem cells (MSCs) to treat osteoporosis. MSCs were labeled and injected intravenously into rats. The cells distributed mainly to the lungs and lymphoid organs and did not preferentially home to bone tissue. While locally injected MSCs participated in bone formation, systemic or local injection did not prevent bone loss in osteoporotic rats. The failure suggests that the osteoporotic environment does not provide enough stimulus for the MSCs to differentiate into osteoblasts and form new bone. Combining MSCs with osteoinductive carriers or growth factors may be needed to achieve bone regeneration.
042 introducing a novel model
This document summarizes studies examining methods to track macrophage recruitment into atherosclerotic plaques. Initial EM studies in pigs observed bi-directional movement of foam cells through the endothelium. Subsequent studies labeled monocytes with fluorescent dyes or microspheres and tracked their migration into plaques in pigs and mice. A newer method used PCR to detect transfusion of male monocytes into female mice. The presented study examined the effects of cytokines on monocyte homing in apoE knockout mice, finding greater iron particle deposition from injected SPIO nanoparticles in cytokine-treated mice, indicating increased monocyte recruitment. Non-invasive SPIO imaging offers potential to sequentially study monocyte recruitment dynamics into plaques.
Ea smac homing-oral
This document summarizes studies examining methods to track macrophage recruitment into atherosclerotic plaques. Initial EM studies in pigs observed bi-directional movement of foam cells through the endothelium. Subsequent studies labeled monocytes with fluorescent dyes or microspheres and tracked their migration into plaques in pigs and mice. A newer method used PCR to detect transfusion of male monocytes into female mice. The presented study examined the effects of cytokines on monocyte homing in apoE knockout mice, finding greater iron particle deposition from injected SPIO nanoparticles in cytokine-treated mice, indicating increased monocyte recruitment. Non-invasive SPIO imaging offers potential to sequentially study monocyte recruitment dynamics into plaques.
DVA Research Poster
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Thesis Defense
The document discusses research on using mechanostimulated Wharton's jelly stem cells seeded into human umbilical veins for tendon tissue engineering applications. Previous research found that seeding rat mesenchymal stem cells into human umbilical veins and applying mechanical stimulation improved the constructs' biomechanical properties and cellularity over static controls. The current research seeds human Wharton's jelly stem cells into umbilical veins and applies varying frequencies and durations of mechanical stimulation. Preliminary results found tenomodulin expression increased with stimulation, and collagen I expression increased most with less rigorous stimulation. Lower seeding densities and addition of growth factors may further improve the constructs for tendon tissue engineering.
Mouse model: Pros & Cons
The document discusses mouse models as experimental systems for studying human disease. It notes that mice are commonly used due to their similarities to humans at the genomic, physiological and developmental levels. However, it also outlines some limitations of mouse models, such as poor translation of drug responses to humans and inability to model certain human diseases and behaviors. The document advocates for improving existing models and exploring alternatives like humanized mouse models and stem cell-based models to better mimic human conditions.
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AALAS ALAT Chapter 1 History
This chapter provides a summary of the history of laboratory animal science and the role of animal care programs. It discusses the early observations and experiments conducted on animals by scientists such as Aristotle and Galen. It then outlines the formation of organizations like AALAS and guidelines like the Three Rs (replacement, refinement, reduction) to promote ethical animal research. The chapter emphasizes that advances in medicine have benefited both humans and animals due to research using animals. It concludes by describing the role of the Assistant Laboratory Animal Technician in providing daily animal care and husbandry essential to research.
Alternatives to the use of animals in experimentations
This document discusses the importance of animal ethics in scientific research and the need for effective implementation of the 4Rs - refinement, replacement, reduction, and rehabilitation. It outlines various international acts and guidelines that are followed to protect animal welfare, such as the ICH, CPCSEA, NIH, and OECD guidelines. The principles of the 4Rs are explained in more detail, focusing on replacement methods like cell culture, QSAR models, and CAL programs that can be substituted for animal testing. The document concludes that continued development of alternative technologies and substitutions for animal testing through the 4Rs can help achieve successful disease treatments without the use of animals.
Animal house managemant
This document provides guidelines for the proper design and management of an animal house. It discusses key factors that must be considered like housing different species separately, maintaining proper temperature, humidity and noise levels. Proper veterinary care of the animals and maintenance of detailed records on staff training, animal health and procurement are also highlighted. The overall guidelines are intended to ensure quality care of the animals used for research studies.
Anaesthesia and euthanasia
This document provides background information on assessing and alleviating animal pain during research procedures. It discusses how animal pain can be recognized through behavioral and physiological changes. The importance of adequate post-procedure care like analgesia administration and prevention of complications is outlined. Common anesthetics used in laboratory animals like chloralose, urethane, barbiturates, paraldehyde, magnesium sulfate and ketamine are defined and their typical dosages for species like dogs, cats, rabbits and rats are provided. Factors affecting anesthetic activity and general considerations for anesthesia are also summarized.
Clinical pathophysiology f toxic plants
This document provides an overview of clinical and pathological effects of toxic plants. It begins with an introduction and classifications of toxic plants. It then discusses the clinical and pathophysiological effects of toxic plants, including specific plants that can cause various types of poisonings. The document covers various plant metabolites and toxins, including alkaloids, terpenes, glycosides, and others. It concludes with treatments and management of plant poisonings.
Expt. ani. models
This document provides an overview of various experimental animal models that are used to induce different disease conditions and evaluate potential treatments. It discusses models for inflammatory diseases, pyrexia, arrhythmias, hypertension, diabetes, hyperlipidemia, and tests for assessing central nervous system activity, muscle relaxation, sedation, anxiety, seizures, convulsions, and analgesia. Examples of specific animal models and procedures are provided for each condition. The models described allow for studying disease pathogenesis and testing new drug candidates before human trials.
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Alternative to Animal Experiment Models
The document discusses alternatives to animal experimentation. It provides an overview of animal experimentation, including its historical use and current regulatory guidelines. Some key uses of animals in experimentation include education, research, cosmetic testing, and toxicology testing. The document then discusses the development of alternatives such as in vitro techniques like cell cultures, microorganism studies, computer simulations, and epidemiological research that can replace or reduce animal use. It provides examples of specific alternative tests and methods that have been validated including embryonic stem cell tests, the Ames test, and skin patch tests. Overall, the document promotes developing and validating alternative methods to animal testing that satisfy the principles of replacement, reduction and refinement of animal use.
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Introduction to alternatives to animal testing in toxicology
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This document provides an introduction to general toxicology using the zebrafish egg model. It discusses the author's background working with alternative test methods and regulatory affairs. The principles of toxicology are outlined, including common in vivo models and alternative test methods. The zebrafish egg model is presented as a universal model for applications like acute toxicity testing, developmental toxicity assessment, safety pharmacology, and mammalian organotoxicity prediction. Protocols for zebrafish husbandry, egg production, development stages, and teratogenicity testing are described.6
1) Treatment with 50 nM of the histone deacetylase inhibitor trichostatin A (TSA) improved the developmental competence of interspecies somatic cell nuclear transfer (iSCNT) embryos reconstructed from cat somatic cells and bovine oocytes.
2) TSA treatment at 50 nM resulted in significantly higher rates of cleavage and blastocyst formation compared to untreated iSCNT embryos.
3) TSA treatment at 50 nM increased histone H3 lysine 9 (H3K9) acetylation levels in iSCNT embryos to levels similar to in vitro fertilized embryos, whereas untreated iSCNT embryos had lower acetylation levels.
Haematological and Serum Biochemical Parameters of Mature Harco Cocks Treated...
Twenty sexually matured (24 weeks old) healthy Harco cocks were used to determine the effect of Gonadotrophin (Diclair®) on haematology and serum biochemistry. The cocks were divided into 4 treatment groups of 5 cocks per group identified as T1 (control) administered with 1ml physiological saline, T2, administered with 6.75i.u Diclair® and T4, administered with 20.25i.u Diclair®, with one cock per replicate in a completely Randomized Design (CRD). The injections were dividedinto three doses each and administered intramuscularly in the thigh for three consecutive days. One week after Diclair® treatments, five birds from each group were bled from the wing veins for haematology and serum biochemistry. Results of this study showed significant differences (P<0.05)>0.05) among the treatment groups. Basophils were not detected among the treatment groups. The results further showed significant differences (P<0.05)>0.05) among the treatment groups. However, the values were within the normal ranges, indicating that Diclair® had no deleterious effect on these parameters.
Mice as an experimental animal
Mice are commonly used as experimental animals in research due to their genomic and physiological similarities to humans, low cost, short lifespan, and ability to be bred easily in a laboratory setting. There are several types of mice used, including inbred strains like C57BL/6 and BALB/c, with BALB/c being the most widely used strain. Mice are classified by age, from neonatal to aged adults. Transgenic and knockout mouse models also exist where genes are inserted or removed to study their functions. Mice are useful for studies in areas like toxicity, teratogenicity, genetics, cancer, and the screening of drugs.
Hammerman Xenotransplantation of organ primordia Curr Opin Org TX 2014
Organ primordia from pig embryos have been transplanted with some success to treat end-stage renal disease and diabetes in animal models. Embryonic kidney primordia transplanted into rats differentiated into functioning kidneys that attracted a blood supply from the host, though immune suppression was required. Embryonic pancreas primordia obtained very early in development engrafted long-term in diabetic rats and rhesus macaques without immune suppression, correcting glucose levels. Later attempts involved transplanting adult porcine islets into animals that had previously received embryonic pancreas transplants, to supplement insulin production.
Transgenic animals and process to make transgenic animals
The document summarizes topics related to transgenic animals and gene therapy. It discusses transgenic cows, sheep, poultry, and fish. For each animal, it describes the process used to create transgenic versions, including pronuclear microinjection and somatic cell nuclear transfer. Benefits include producing human therapeutic proteins and altering milk composition. Challenges include high costs and low success rates. Gene therapy techniques like viral vectors and electroporation are explained for inserting genes into tissues to treat disease. Somatic gene therapy aims to modify individual patients while germline gene therapy alters heritable genes passed to offspring.
Stem Cells in the Treatment of Osteoporosis
This study investigated using mesenchymal stem cells (MSCs) to treat osteoporosis. MSCs were labeled and injected intravenously into rats. The cells distributed mainly to the lungs and lymphoid organs and did not preferentially home to bone tissue. While locally injected MSCs participated in bone formation, systemic or local injection did not prevent bone loss in osteoporotic rats. The failure suggests that the osteoporotic environment does not provide enough stimulus for the MSCs to differentiate into osteoblasts and form new bone. Combining MSCs with osteoinductive carriers or growth factors may be needed to achieve bone regeneration.
042 introducing a novel model
This document summarizes studies examining methods to track macrophage recruitment into atherosclerotic plaques. Initial EM studies in pigs observed bi-directional movement of foam cells through the endothelium. Subsequent studies labeled monocytes with fluorescent dyes or microspheres and tracked their migration into plaques in pigs and mice. A newer method used PCR to detect transfusion of male monocytes into female mice. The presented study examined the effects of cytokines on monocyte homing in apoE knockout mice, finding greater iron particle deposition from injected SPIO nanoparticles in cytokine-treated mice, indicating increased monocyte recruitment. Non-invasive SPIO imaging offers potential to sequentially study monocyte recruitment dynamics into plaques.
Ea smac homing-oral
This document summarizes studies examining methods to track macrophage recruitment into atherosclerotic plaques. Initial EM studies in pigs observed bi-directional movement of foam cells through the endothelium. Subsequent studies labeled monocytes with fluorescent dyes or microspheres and tracked their migration into plaques in pigs and mice. A newer method used PCR to detect transfusion of male monocytes into female mice. The presented study examined the effects of cytokines on monocyte homing in apoE knockout mice, finding greater iron particle deposition from injected SPIO nanoparticles in cytokine-treated mice, indicating increased monocyte recruitment. Non-invasive SPIO imaging offers potential to sequentially study monocyte recruitment dynamics into plaques.
DVA Research Poster
The study aimed to test the effects of commercial deer velvet antler (DVA) and insulin-like growth factor 1 (IGF-1) on cell proliferation using 3T3 mouse fibroblast cells. Scratch assays were performed with DVA, IGF-1, and their combination in media with and without serum. The assays found increased cell proliferation at higher concentrations of the substances. However, results from follow-up MTT assays to confirm the effects were inconclusive. Further scratch assays and MTT assays are needed to fully support the hypothesis that DVA enhances cell proliferation similarly to IGF-1.
Thesis Defense
The document discusses research on using mechanostimulated Wharton's jelly stem cells seeded into human umbilical veins for tendon tissue engineering applications. Previous research found that seeding rat mesenchymal stem cells into human umbilical veins and applying mechanical stimulation improved the constructs' biomechanical properties and cellularity over static controls. The current research seeds human Wharton's jelly stem cells into umbilical veins and applies varying frequencies and durations of mechanical stimulation. Preliminary results found tenomodulin expression increased with stimulation, and collagen I expression increased most with less rigorous stimulation. Lower seeding densities and addition of growth factors may further improve the constructs for tendon tissue engineering.
Mouse model: Pros & Cons
The document discusses mouse models as experimental systems for studying human disease. It notes that mice are commonly used due to their similarities to humans at the genomic, physiological and developmental levels. However, it also outlines some limitations of mouse models, such as poor translation of drug responses to humans and inability to model certain human diseases and behaviors. The document advocates for improving existing models and exploring alternatives like humanized mouse models and stem cell-based models to better mimic human conditions.
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AALAS ALAT Chapter 1 History
This chapter provides a summary of the history of laboratory animal science and the role of animal care programs. It discusses the early observations and experiments conducted on animals by scientists such as Aristotle and Galen. It then outlines the formation of organizations like AALAS and guidelines like the Three Rs (replacement, refinement, reduction) to promote ethical animal research. The chapter emphasizes that advances in medicine have benefited both humans and animals due to research using animals. It concludes by describing the role of the Assistant Laboratory Animal Technician in providing daily animal care and husbandry essential to research.
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This document discusses the importance of animal ethics in scientific research and the need for effective implementation of the 4Rs - refinement, replacement, reduction, and rehabilitation. It outlines various international acts and guidelines that are followed to protect animal welfare, such as the ICH, CPCSEA, NIH, and OECD guidelines. The principles of the 4Rs are explained in more detail, focusing on replacement methods like cell culture, QSAR models, and CAL programs that can be substituted for animal testing. The document concludes that continued development of alternative technologies and substitutions for animal testing through the 4Rs can help achieve successful disease treatments without the use of animals.
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Anaesthesia and euthanasia
This document provides background information on assessing and alleviating animal pain during research procedures. It discusses how animal pain can be recognized through behavioral and physiological changes. The importance of adequate post-procedure care like analgesia administration and prevention of complications is outlined. Common anesthetics used in laboratory animals like chloralose, urethane, barbiturates, paraldehyde, magnesium sulfate and ketamine are defined and their typical dosages for species like dogs, cats, rabbits and rats are provided. Factors affecting anesthetic activity and general considerations for anesthesia are also summarized.
Clinical pathophysiology f toxic plants
This document provides an overview of clinical and pathological effects of toxic plants. It begins with an introduction and classifications of toxic plants. It then discusses the clinical and pathophysiological effects of toxic plants, including specific plants that can cause various types of poisonings. The document covers various plant metabolites and toxins, including alkaloids, terpenes, glycosides, and others. It concludes with treatments and management of plant poisonings.
Expt. ani. models
This document provides an overview of various experimental animal models that are used to induce different disease conditions and evaluate potential treatments. It discusses models for inflammatory diseases, pyrexia, arrhythmias, hypertension, diabetes, hyperlipidemia, and tests for assessing central nervous system activity, muscle relaxation, sedation, anxiety, seizures, convulsions, and analgesia. Examples of specific animal models and procedures are provided for each condition. The models described allow for studying disease pathogenesis and testing new drug candidates before human trials.
CPCSEA ppt final (S S C)
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Cpcsea guidelines for laboratory animal facility
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The document summarizes guidelines from the Committee for the Purpose of Control and Supervision on Experiments on Animals (CPCSEA) regarding the care and use of animals for research purposes. It outlines provisions for veterinary care, quarantine, food/water/bedding, sanitation, facilities, transportation, anesthesia, euthanasia, record keeping, and standard operating procedures. The guidelines aim to promote the humane treatment of laboratory animals used for biomedical and behavioral research experiments.
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This document discusses the key steps and principles of histotechniques, which is the process of preparing tissue for microscopic examination. The main steps described are:
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The document emphasizes best practices for fixation like using the proper fixative concentration and duration tailored to the specific tissue type and intended analysis. Artifacts from improper
Alternatives to animal experiments
The document discusses alternatives to animal experiments that can be used in biomedical research and testing. It covers 3R strategies like refinement, reduction and replacement of animal experiments. Some alternatives mentioned include in vitro cell and tissue culture methods, computer-based models, microdosing studies and quantitative structure-activity relationships. The summary provides an overview of the different alternative methods discussed in the document like in vitro toxicity testing, in chemico tests, computer-assisted learning programs, microfluidic chips and in silico models. The use of these alternatives can help reduce animal experiments while making toxicity testing more accurate and reliable.
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SUB ACUTE ORAL TOXICITY
Ethynyl estradiol was evaluated for sub-acute oral toxicity in a repeated 28-day study using rats in accordance with OECD 407 guidelines. Rats were divided into four groups that received daily doses of either an olive oil solution (control), 10 μg/kg, 50 μg/kg, or 200 μg/kg of ethynyl estradiol by oral gavage. Parameters evaluated included body weight, food consumption, hematology, histopathology, spermatology, and estrous cycling. Results showed reduced food consumption and body weight gain in males at 200 μg/kg. Hematological changes and abnormal estrous cycling were also observed at higher doses.
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The pancreas is densely innervated, and neural signals play a significant role in glucose regulation by modulating pancreatic hormone release. However, relatively little is known about the anatomical relationships between islets and nerves across the whole pancreas. In this webinar, Dr. Sarah Stanley and Dr. Alexandra Alvarsson will discuss their research using tissue clearing and whole organ imaging of the pancreas to identify the 3D structure of pancreatic nerves and islets.
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Transgenic animal (pharmacology) (M.PHARM)
A transgenic animal is one that carries a foreign gene that has been deliberately inserted into its genome.
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Mouse – common transgenic expt.
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Ali lahko celični modeli nadomestijo laboratorijske živali?
Avrelija Cencič
Ali lahko celični modeli nadomestijo laboratorijske živali?
--
21. 12. | 17.00 | Miklošičeva dvorana UM
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33133 article text-60219-2-10-20211104
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Reversible antifertility effect_of_cassia_tora_linn_in_male_rats
Reversible antifertility effect_of_cassia_tora_linn_in_male_ratsSSR Institute of International Journal of Life Sciences
Reversible Antifertility Effect of Cassia tora Linn in Male Rats
Samiya Khan, Pratap Chand Mali*
*Address for Correspondence: Dr. Pratap Chand Mali, Associate Professor, Reproductive Biomedicine and Natural Products Lab, Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur, India
ABSTRACT- Background: Plant Cassia tora has been used in traditional and modern medicines for different pharmacological activities.
Objectives: The present investigation has been taken to observe and evaluate effects of Cassia tora on the reproduction functions of male rats in search a safe, orally effective and reversible fertility regulating agent.
Materials and Methods: Fifty percent ethanolic extract of Cassia tora was prepared and administered orally in male Wistar rats at the doses of 50, 100 and 200 mg/kg.b.wt./rat/day dose levels respectively for a period of 60 days and some of the treated rats were kept 30 days for recovery of fertility to assessed reversibility effects. Hematological indices, serum clinical investigations were also performed to assess toxic effects if any caused in rats by treatment. Proteins, cholesterol, glycogen, ascorbic acid, sialic acid and fructose level were analyzed in rats. Serum FSH, LH and Testosterone levels were measure. Rats were castrated to evaluate effects on reproductive functions of hormones and mode of action of the Cassia tora treatment. For histopathological observations tissues were fixed in Bouin’s fluid, dehydrated, sectioned and stained with Hematoxylin and Eosin.
Results: Treatment of Cassia tora significantly reduced the weights of testes and accessory sex organs. Sperm density and motility were declined high significantly. Levels of Testosterone and FSH hormone were significantly decreased in rats. The protein, sialic acid, fructose, ascorbic acid and glycogen contents of reproductive accessory sex organs were decreased significantly. Germinal epithelium of testes degenerated and number of spermatocytes, spermatids and spermatozoa in lumen of seminiferous tubules reduced.
Conclusions: The decreased testes and accessory sex organs weights, sperm motility, density and testosterone level in rats might be due to androgen suppression effects of Cassia tora treatment cause inhibition of spermatogenesis resulted reduction of fertility in treated male rats.
Key-words- Cassia tora, Contraception, Fertility, Sperm motility, Sperm density, Male rat
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原件一模一样【微信：WP101A】【澳洲纽卡斯尔大学毕业证（UoN学位证）成绩单】【微信：WP101A】（留信学历认证永久存档查询）采用学校原版纸张、特殊工艺完全按照原版一比一制作（包括：隐形水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠，文字图案浮雕，激光镭射，紫外荧光，温感，复印防伪）行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备，十五年致力于帮助留学生解决难题，业务范围有加拿大、英国、澳洲、韩国、美国、新加坡，新西兰等学历材料，包您满意。
【业务选择办理准则】
一、工作未确定，回国需先给父母、亲戚朋友看下文凭的情况，办理一份就读学校的毕业证【微信：WP101A】文凭即可
二、回国进私企、外企、自己做生意的情况，这些单位是不查询毕业证真伪的，而且国内没有渠道去查询国外文凭的真假，也不需要提供真实教育部认证。鉴于此，办理一份毕业证【微信：WP101A】即可
三、进国企，银行，事业单位，考公务员等等，这些单位是必需要提供真实教育部认证的，办理教育部认证所需资料众多且烦琐，所有材料您都必须提供原件，我们凭借丰富的经验，快捷的绿色通道帮您快速整合材料，让您少走弯路。
留信网认证的作用:
1:该专业认证可证明留学生真实身份【微信：WP101A】
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
→ 【关于价格问题（保证一手价格）
我们所定的价格是非常合理的，而且我们现在做得单子大多数都是代理和回头客户介绍的所以一般现在有新的单子 我给客户的都是第一手的代理价格，因为我想坦诚对待大家 不想跟大家在价格方面浪费时间
对于老客户或者被老客户介绍过来的朋友，我们都会适当给一些优惠。
选择实体注册公司办理，更放心，更安全！我们的承诺：可来公司面谈，可签订合同，会陪同客户一起到教育部认证窗口递交认证材料，客户在教育部官方认证查询网站查询到认证通过结果后付款，不成功不收费！
办理澳洲纽卡斯尔大学毕业证（UoN学位证）学位证【微信：WP101A 】外观非常精致，由特殊纸质材料制成，上面印有校徽、校名、毕业生姓名、专业等信息。
办理澳洲纽卡斯尔大学毕业证（UoN学位证）学位证【微信：WP101A 】格式相对统一，各专业都有相应的模板。通常包括以下部分：
校徽：象征着学校的荣誉和传承。
校名:学校英文全称
授予学位：本部分将注明获得的具体学位名称。
毕业生姓名：这是最重要的信息之一，标志着该证书是由特定人员获得的。
颁发日期：这是毕业正式生效的时间，也代表着毕业生学业的结束。
其他信息：根据不同的专业和学位，可能会有一些特定的信息或章节。
办理澳洲纽卡斯尔大学毕业证（UoN学位证）学位证【微信：WP101A 】价值很高，需要妥善保管。一般来说，应放置在安全、干燥、防潮的地方，避免长时间暴露在阳光下。如需使用，最好使用复印件而不是原件，以免丢失。
综上所述，办理澳洲纽卡斯尔大学毕业证（UoN学位证）学位证【微信：WP101A 】是证明身份和学历的高价值文件。外观简单庄重，格式统一，包括重要的个人信息和发布日期。对持有人来说，妥善保管是非常重要的。
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PK Services
- 1. PK Services Animal Facility GVK BIO vivarium is AAALAC certified and registered with CPCSEA (Committee for the Purpose of Control and Supervision of Experiments on Animals), Government of India. In our rodent facilities, animals are housed in Individual Ventilated Cages (IVC) in controlled temperature (22° -25°C) & relative humidity (30%-70%) maintained at 12 h/12 h light/dark cycle. In Vivo Pharmacokinetics • Rats of different strains- Sprague Dawley(SD), Wistar, Wistar Han and Lewis • Mice of different strains - Swiss albino, BALB/c, C57BL/6, CD-1, Nude mice, etc. • Hamster and Guinea pigs • Discrete/Serial/Drug- Drug Interaction • Rapid PK/Snapshot PK/Cassette PK • Absolute Bioavailability • Capabilities to conduct PK/PD relationship studies • Blood sample collection techniques – retro-orbital / cardiac puncture/tail vein and jugular vein • In Vivo Blood Brain Barrier(BBB) study • Tissue Distribution studies • Specialized studies for enterohepatic/first pass effect • Ex vivo protein binding studies to measure free/bound drug and unbound drug in plasma, brain and CSF • Ex vivo tissue permeability studies -Franz Diffusion assay (PermeGear) • Biliary excretion studies • Toxicokinetic studies • Perfusion capabilities Cannulation/Surgical Procedures • Jugular vein cannulation • Portal vein cannulation • Bile duct cannulation • Femoral vein cannulation • Double cannulation (jugular and femoral) • Double cannulation (jugular and portal, jugular and bile duct • Carotid artery cannulation • Ovarectomy • Adrenalectomy Routes of Administration • Oral - Solution, Suspension, Solids (pellets, capsules) • Intravenous - Bolus, Infusion • Intraperitoneal • Subcutaneous • Intramuscular • Dermal • Intratracheal • Buccal/Sublingual • Intraduodenal • Intracolonic • Intranasal Type of PK Studies • Single Dose • Multiple Dose • Dose Linearity • Tissue Distribution • Alternate Dosing • Drug-Drug Interaction Studies • Fed/Fast PK • BBB • BBB & CSF • Topical PK • Infusion Studies • Cassette PK Biological Fluid/Organ Collection • Blood • Urine • CSF (Rat) • BAL • Bile • All organs (brain, lungs, heart, lymphnode, salivary gland, ovary, spleen, skin, muscle, adrenals, kidney, fat tissue, thymus etc.) We provide flexibility in protocol development, quality data and rapid turnaround time to our clients.
- 2. PK Services Bio-analysis • • • • • Analysis by LC-MS/MS/HPLC-UV/PDA/ Fluorimetry/ Spectrophotometer Method Development and Validation in Biological Matrices such as Blood, Plasma/Serum, CSF and Tissue homogenates PK analysis by Watson LIMS Quantification of Parent and Metabolites, Cassette Analysis, Cell Culture Measurement of Parent and Prodrug Typical study design for In Vivo PK/Bio-analysis Report delivery within seven working days Administration Route Animal Protocol p.o.,i.v., i.p., s.c., i.m. infusion Mice/Rats (N=3/group) Parameters Data Analysis Blood/Tissue samples collected at scheduled time points post dosing, plasma separated and analyzed for test item concentration Cmax, Tmax, AUC, clearance, terminal elimination half life, bioavailiability and volume of distribution WinNonlin Software AUC (ng.hr/g) 60000 50000 40000 30000 c BBB –CSF study of test compound in SD rat at 10 mg/kg following oral administration. c BBB –CSF 20000 10000 Adrenal … Blood Brain Heart Fat Lung Liver Kidney Lymph Salivary Pancreas Testis Muscle Spleen 0 BBB –CSF study of test compound in SD rat at 10 mg/kg following oral BBB-CSF study of Test compound in SD Rats (10 mg/kg) following oral administration. Tissue distribution profile the test compound in Wistar rat Tissue distribution profile of of the Test compound in Wistar Rats c BBB –CSF study of test compound in SD rat at 10 mg/kg f ollowing oral administration. 750 c BBB –CSF study of test compound in SD rat at 10 mg/kg following oral c BBB –CSF study of test compound in SD rat at 10 mg/kg following oral administration. administration. Concentration (ng/mL) Prodrug 500 Drug 250 0 0 0.1 0.2 Time (hr) 0.3 Bioavailability compound in male Sprague Dawley rat after intravenous (1mg/kg) Bioavailability ofof Test compound in male SD Rats after intravenous (1mg/kg) and oral (5mg/kg) administration and oral (5mg/kg) administration. The pharmacokinetic profile of Prodrug and parent compound in blood inmale Wistar rat Pharmacokinetic profile of Prodrug and Parent compound in blood in male Wistar after intraportal administration of Prodrug at a dose of 3 mg/kg Rats after intraportal administration of Prodrug (3 mg/kg) About GVK BIO GVK Biosciences (GVK BIO) is Asia’s leading Discovery Research and Development organization. GVK BIO provides a broad spectrum of services, stand-alone and integrated, across the R&D value chain. Chemistry Services ▪ Process R&D and Custom Synthesis ▪ Biology ▪ Informatics ▪ Clinical Research ▪ Clinical Pharmacology Contact: bdbio@gvkbio.com Visit: www.gvkbio.com