2. Introduction of institution:
The first comprehensive cancer
Center in Pakistan but a center
Where all patients receive multi-
-disciplinary treatment. SKMCH
has established itself as a center
Of excellence. It was founded by
Imran khan in 1994.
Anam yousaf
(2010-am-006)

3. Content list
Blood bank
Microbiology
Molecular biology
Cytogenetic
Histopathology
Cytopathology
Hematology

4. Blood bank section:
Blood donation area
• Blood collection
• Plateletpheresis
Laboratory area
• ABO Blood grouping
• Cross matching
• RPR test
• Cell separator

5. Microbiology section:
• Urine analysis
• Culture & sensitivity
• Culture reading
• Biochemical tests
• Staining
• Kit based tests
• (for stool samples)
• API
• Bactec 9240

6. Molecular biology section:
PCR section
• Extraction & amplification
• FISH for HER-2/neu gene
Genotyping section
• Genotyping of HCV
RNA extraction
PCR
Linear Array HCV genotyping detection kit

7. Cytogenetics section:
Cytogenetic for acquired disorders
 samples:
• Blood
• Bone marrow
 Processing:
• Cell counting & culturing
• Harvesting & staining
• Cytovision system

8. Histopathology section:
Grossing of different body tissues
Core & mucosal biopsies
Processing in Leica peloris processing unit
Embedding in Leica EG
Sectioning
Slide preparation & staining
H & E staining

9. Sectioning
by
microtome
Processing
(embeddin
g)
grossing

10. Cytopathology section:
• Samples: Staining:
• CSF Giemsa staining
• FNAs Rapi staining
• Body fluids PAP staining
• Sputum Reporting:
• Blood Microscopy
• Urine
• Semen analysis

11. Hematology section:
blood & CSF samples
• CBC + auto differential
• Manual differential by slide
• Cell counts of cells
• Coagulation factors
• ESR
• Flow cytometry for peripheral
blood
 Bone marrow screening:
 Four types of slides:
 Bone marrow aspirate
 Touch preparation
 Peripheral blood
 Trephine biopsy
 Staining:
 Giemsa staining
 Iron staining

12. CBC ( Auto diff) &
Sysmex coagulation
detector
Flow cytometer, Hema
tek stainer & ESR (
Western Gren)

13. Suggestions and future plans
 there should be no internship program. Instead of this research
work with thesis will be more productive for the students.
Will continue my studies in sha ALLAH