Lonza Bioscience provides a variety of life science research products and services including cell culture media, reagents, assays, primary cells, stem cells, and cell therapy services. They have expertise in areas such as cell culture, cell biology, bioassays, electrophoresis, and rapid microbial detection. Lonza Bioscience manufactures most of their offerings and maintains high quality standards in compliance with cGMP. They aim to support customers through technical services and reliable customer service.

1. Lonza Bioscience
Merck Boston
Ernie Desmarais 8/21/07

2. Lonza Bioscience Capabilities

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Table of Contents
Lonza Bioscience Overview
Research Products
Biotherapeutic Media Products
Rapid Microbial Detection
Cell Therapy Bioservices

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Lonza Bioscience Overview
Lonza Bioscience provides a variety of products and services for
life science research, quality control of pharmaceutical products for
injection, cell culture media for bioprocessing and cell therapy
services.
We Are Specialists In:
 Cell Culture
 Cell Biology
 Bioassays
 Electrophoresis
 Rapid Microbial Detection

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Bioscience is crucial to therapeutic innovation

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Lonza Bioscience Service & Support
Customer Service
 US: Customer Service in Walkersville, MD
and Rockland, ME
 Europe: Customer Service in Belgium,
Spain, Italy and England
 Phone Service M-F, 8:00am - 6:00pm
 E-Commerce, 24/7
Technical Services Group
 US and Europe
 Provide technical assistance and information
 Product experts
 Manage product testing, investigations and
Material Review Board

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Lonza Bioscience Quality Systems
Our entire staff is dedicated to maintaining the highest
compliance standards in the industry including:
All necessary requirements for cGMP compliance
QA review and approval of manufacturing documents
Comprehensive internal audit program
Raw materials release
Vendor audit and qualification programs
Reviewing and approving all product labeling and documents associated
with manufactured products based on FDA, EU and/or other current
applicable regulatory guidelines
Coordination of Corrective and Preventive Action Programs (CAPA)
Liaison to regulatory agencies and client
Regulatory support

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Research Products Overview
 Products and Services for Life Science Research
 Over 75 years of experience in cell culture:
 Primary Cells
 Classical Media & Sera
 Serum-Free Media
 Cell Therapy
 Integrated Platform of Cells, Media and BioAssays
 Over 40 years of experience in electrophoresis
 Agarose and Precast Gels
 Protein Precast Gels
 Basic manufacturer of >95% of offerings

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Research Products Overview (continued)
 Customers include:
 Academic and government institutions
 Research hospitals and medical schools
 Biotechnology companies
 Pharmaceutical companies
 Global presence
 Direct sales in 18 countries
 Distributors throughout the world
 Dedicated Technical Service

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Cell Biology Overview
Leader in primary cells and media systems
State-of-the-art cell isolation laboratory
Over 100 human and animal primary cell
types available
Proprietary growth and cryopreservation
protocols
Custom cell isolation capabilities
Extensive sources and experience in ethical
procurement of human and animal tissue
We offer performance and convenience:
We procure the tissue
We isolate and qualify the cells
We deliver the cells to you

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Primary Cells & Media
Clonetics®
Cells and Media Systems
Cells are non-immortalized, non-transformed and
produced from normal tissue
Advantages
Greater relevance – no species extrapolation, alternative to
animal testing, more relevant results
High quality – standardized isolation protocols and extensive
testing assure predictable results
Outstanding performance – optimized media systems drive
cell expansion yet maintain normal cell characteristics
Convenience – we procure the tissue and perform the
isolations under standardized, controlled conditions
Trusted source – ethical, documented tissue acquisition and
rigorous quality standards

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Primary Cells & Media (continued)
Available cyropreserved or as growing cultures
Specialized media optimized for superior
performance
Human cells tested for HIV, Hepatitis B and C as
well as for mycoplasma and sterility
Products are quality controlled and issued with a
certificate of analysis

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Primary Cell Systems Offered
Airway Cell Systems
Bladder Cell Systems
Endothelial Cell Systems
Fibroblast Cell Systems
Keratinocyte Cell Systems
Mammary Epithelial Cell
Systems
Smooth Muscle Cell Systems
Skeletal Muscle Cell Systems
Human and Animal Neural
Cell Systems
Prostate Cell Systems
Skeletal Cell Systems
Hepatocyte Cell Systems
Renal Epithelial Cell Model
Blood Brain Barrier Model
(Bovine)

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Stem Cell Systems
Poietics®
Stem Cells and Media
 Poietics®
products include a collection of adult stem cells
derived from the hematopoietic system and other tissues
 Fresh or cryopreserved cells are available from bone
marrow, G-CSF mobilized peripheral blood, cord blood, and
fetal liver
 Obtained through Institutional Review Board approved
donor programs
 Optimized media are available for growth and differentiation
 Highly purified populations from proprietary positive and
negative selection protocols
 All products are quality controlled and issued with a
certificate of analysis

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Stem Cell Systems (continued)
Preadipocyte Cell and Media Systems
Visceral Preadipocytes
Subcutaneous Preadipocytes
Osteoclast Precursors
Neural Progenitor Cells and Media
Human Bone Marrow
Mononuclear Cells from multiple sources
Hematopoietic Progenitors from multiple sources
Erythroid Progenitors
Mesenchymal Stem Cell System
Immune System Cells and Media
CD14+ Monocytes
Stromal Cells from Human Bone Marrow
Quadratox™ 4 Species Panel

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Custom Cell Isolation
Custom Cell Isolation Services
Extensive experience with multiple human and
animal cell types
Broad sourcing capabilities
Packaged to your unique specifications:
Cell count
Pre-seeded multi-well plates
Pre-seeded flasks
Cryopreserved
Customized treatments available:
Cell pellets
Specialized media
Customer specified cell treatment

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Cell Culture
Broadest range of cell culture applications
Bench research
High throughput screening
Production of biological therapeutics
Production of cell therapy products
Production of diagnostic tests
Cell culture products are manufactured under cGMP
protocols
Production is controlled from raw materials to
finished goods
All products are manufactured with WFI (water for
injection) quality water
Media is sterile filtered using pharmaceutical-grade
filters (USP or EP sterility)
Sold as BioWhittaker™ brand

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Specialty Media
Serum-free, protein-free, chemically
defined and NAO media
Advantages of specialty media
formulations
Increased lot-to-lot consistency
Simplified purification and
downstream processing
Better control over physiological
conditions of cultures
Ability to optimize formulations for
specific cell types

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Specialty Media (continued)
Media systems are available to optimize performance in:
CHO cells (PowerCHO™, ProCHO™, and UltraCHO™ media)
Immune cells (X-VIVO™ media)
Kidney cells (UltraMDCK™, Pro293™, and ProVero™ 1 media)
Insect cells (Insect-EXPRESS™ medium)
Hybridoma cells (HL-1™, UltraDOMA™, and ProDoma™ media)
ES Cells (X-VIVO™ media + growth factors)
General purpose serum-free media
UltraCULTURE™, PC-1™, and UltraMEM™ media
Other applications (available in Europe only)
Karyotyping, Cytogenetics, In-vitro fertilization

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Classical Media
Broad range of standard media used for
many cell culture applications
Manufactured with Tissue Culture
Association formulas
Manufactured under cGMP protocols
Prepared with WFI water
Available in liquid or powder formulations
Tests performed
Sterility
pH and Osmolality
Cell Growth Promotion on three cell
types (primary, diploid, heteroploid)
Endotoxin

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Cell Culture Reagents
Tissue culture grade products including
amino acids, antibiotics, buffers
Prepared with WFI water
Manufactured under cGMP protocols
Products include:
Buffers
Balanced salt solutions
Antibiotics
Cell harvesting reagents

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Animal & Human Sera
Animal sera is collected from USDA inspected abattoirs in the 48
contiguous states or from USDA certified sources outside of the
United States
US sourced sera are processed using dedicated tanks and
equipment
Human sera is obtained from donors who test negative for
hepatitis B and C, HIV 1 and 2
Sera products are manufactured with cGMP protocols

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Animal & Human Sera (continued)
Sera are tested for:
Mycoplasma
Adventitious virus (animal sera)
Sterility
Endotoxin
Hemoglobin (animal sera)
pH and Osmolality
Biochemical profile (animal sera)
Growth promotion (animal sera)
Products include:
 Fetal Bovine Serum
 Newborn calf serum
 Calf serum
 Donor calf serum
 Equine serum
 Human serum

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BioAssays
Cell health and cytotoxicity assays
Mycoplasma detection assays
Cell function assays
Cell analysis reagents
HTS (high throughput screening) assays
Enzyme assays
GPCRs
Conditionally immortalized cells
Unique, sensitive assays based on
luminescence and fluorescence…

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BioAssays (continued)
Cell health and cytotoxicity assays
ViaLight®
Cell Proliferation and
Cytotoxicity Bioassays
ToxiLight®
Non-Destructive
Cytotoxicity Bioassay
ApoGlow®
Rapid Apoptosis
Screening Kit
Mycoplasma Detection Assay
MycoAlert®
Mycoplasma Detection
Assay Kit - Unique 20 min assay for
mycoplasma contamination detection in
cell cultures

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BioAssays (continued)
Cell Function Assays
OsteoLyse™ Assay (Collagen)
OsteoAssay™ Human Bone Plate
Cell Analysis Reagents
Fluorescent stains for cell organelles, cell structural
elements, and cell tracking

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BioAssays (continued)
Unique products for cell biology research
Transport™ Protein Delivery Reagent
HTS screening assays
PKLight®
HTS Protein Kinase Assay Kit
PDELight®
HTS cAMP Phosphodiesterase Assay Kit
G Protein-Coupled Receptors
Rapidly expanding list of newly de-orphanized
GPCRs
Clonetics®
Conditionally Immortalized Cells
Primary cell performance with telomerase
technology

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Molecular Biology Overview
Integrated platform gels, reagents and buffers for superior
separation of macromolecules
“Ready-to-use” products for protein and nucleic acid separation
Pre-cast gels for nucleic acid electrophoresis
Pre-cast gels for protein electrophoresis
Manufacturer of agarose and agarose derivatives for next
generation chromatography products and large scale protein
purification
World renowned brands in electrophoresis:
SeaKem®
LE Agarose
Reliant ®
Precast Gels
PAGEr®
Gold Gels
Long Ranger®
Sequencing Solutions
GelBond™ Film

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Nucleic Acid Electrophoresis
We provide:
The best known and most widely
cited agarose brands – SeaKem®
,
NuSieve®
and MetaPhor®
We are the inventor and the sole
innovator in agarose technology
for the past 40 years
We use the most stringent requirements in our manufacturing
– FDA and ISO 9001/2000 certifications
Precast Reliant®
and Latitude®
Gels
Precast RNA Gels
RNA & DNA markers and ladders
Nucleic acid stains
Electrophoresis Buffers

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Protein Gel Electrophoresis
High performance protein electrophoresis
products optimized to deliver sharp
resolution, superb sensitivity and fast,
convenient separations
We provide:
PAGEr®
Gold Precast Protein Gels
Protein stains
Protein markers
Electrophoresis buffers

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Sequencing
Superior sequencing performance matched with unique
packaging convenience
Long Ranger®
acrylamide gel solutions for longer
sequencing reads
Long Ranger®
Singel®
Packs – convenience and safety in
casting gels
MDE®
Mutation Detection gels
Sequencing Buffers
GelBond®
Film supports for agarose and acrylamide gels
Durable support for all gel processing steps
Available precut or in rolls

32. Thank You