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BIOPSY AND HEALING OF
THE BIOPSY WOUND
INDIAN DENTAL ACADEMYINDIAN DENTAL ACADEMY
Leader in continuing Dental EducationLeader in continuing Dental Education
www.indiandentalacademy.com
Objectives
At the end of the lecture the student should be
able to-
Define the biopsy.
Enlist Types of biopsy
Portray Indications & Contra-indications
www.indiandentalacademy.com
GREEK
BIO- LIVING, LIFE OPSIS- TO VIEW
It is the removal of tissue from the living organism
for the purpose of microscopic examination and
diagnosis.
TYPES OF BIOPSY -
1. Excisional Biopsy – Total excision of a small
lesion for microscopic study
2. Incisional Biopsy - a small section of a large
lesion removed for microscopic study
www.indiandentalacademy.com
Contraindications
There are no absolute contraindications for
biopsy.
The relative contraindication for biopsy are
◦ Pigmented lesions
◦ Vascular lesions
◦ Uncontrolled diabetes
◦ Lesions prone for infections
www.indiandentalacademy.com
Methods of Biopsy
1. Surgical excision by scalpel
2. Surgical removal by cautery or a high frequency
cutting knife
3. Removal by biopsy forceps or biopsy punch
4. Aspiration through a needle with a large lumen
5. Exfoliative cytology
www.indiandentalacademy.com
Incisional Biopsy
Biopsy that samples only a particular portion or
representative part of a lesion.
If a lesion is large or has different characteristics
in various locations more than one area may
need to be sampled.
www.indiandentalacademy.com
Indications:
 Indicated in larger lesions for diagnosis
(i.e. larger than 1cm in diameter).
 Lesions in hazardous location.
 Indicated for tumors of the soft tissues to
distinguish benign conditions from malignant.
www.indiandentalacademy.com
Erythroplakia
Nodular Leukoplakia
Squamous cell carcinoma
www.indiandentalacademy.com
www.indiandentalacademy.com
EXCISIONAL BIOPSYEXCISIONAL BIOPSY
Total excision of a small lesion for microscopic
study is called excisional biopsy.
 It is preferred if the size of the lesion is small
enough (<1cm), such that it may be removed
along with the margins of normal tissue and
wound can be closed primarily.
www.indiandentalacademy.com
Indications:
Lesions smaller than 1 cm.
If excision does not risk any vital structure.
If clinical impression is that of benign lesion.
If excision can be curative.
www.indiandentalacademy.com
www.indiandentalacademy.com
Exfoliative cytologyExfoliative cytology
It is the study of cells which exfoliate from the
body surfaces
www.indiandentalacademy.com
Methodology
Surface of the lesion is wiped with sponge material
which is then sectioned, or scraped.
Scrape the surface of lesion vigorously with
moistened tongue blade or cytobrush, smeared on
a microscopic slide and fixed with 90% alcohol.
www.indiandentalacademy.com
It is studied by the cytopathologist for presence
of atypical or diagnostic cells
 The cytopathologist may classify the smear
into following classes
www.indiandentalacademy.com
Class I – Normal – normal cells are present
Class II – Atypical – minor atypia but no evidence
of malignant changes
Class III – intermediate- wider atypia that suggest
cancer, but is not clear cut and represent
precancerous lesions- ca in situ- Biopsy is
recommended
www.indiandentalacademy.com
Class IV - suggestive of cancer- few cells with
malignant characteristics or with many cells with
borderline characteristics- Biopsy is
mandatory
Class V - positive of cancer cells are obviously
malignant- Biopsy is mandatory
www.indiandentalacademy.com
PUNCH BIOPSY
Removal of a small disk-shaped sample of tissue.
Used in difficult to access areas like retromolar
trigone, maxillary tuberosity.
Indicated in patients with trismus.
www.indiandentalacademy.com
www.indiandentalacademy.com
Summary
Definition of biopsy, the indications and contra-
indications of biopsy.
Exfoliative cytology, Punch biopsy.
www.indiandentalacademy.com
BIBLIOGRAPHYBIBLIOGRAPHY
Text book of oral pathology Shafer's, 5 & 6th
edition
Color Atlas of Oral Diseases Cawson, R. 2nd
edition
Oral and Maxillofacial Pathology Neville, Brad
W. 2nd
Lucas’s Pathology Of Tumor’s of the Oral
Tissues
Cawson, R. A., Bennie, W. H 5th
edition
www.indiandentalacademy.com
www.indiandentalacademy.com

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BIOPSY AND HEALING OF THE BIOPSY WOUND / dental crown & bridge courses

  • 1. BIOPSY AND HEALING OF THE BIOPSY WOUND INDIAN DENTAL ACADEMYINDIAN DENTAL ACADEMY Leader in continuing Dental EducationLeader in continuing Dental Education www.indiandentalacademy.com
  • 2. Objectives At the end of the lecture the student should be able to- Define the biopsy. Enlist Types of biopsy Portray Indications & Contra-indications www.indiandentalacademy.com
  • 3. GREEK BIO- LIVING, LIFE OPSIS- TO VIEW It is the removal of tissue from the living organism for the purpose of microscopic examination and diagnosis. TYPES OF BIOPSY - 1. Excisional Biopsy – Total excision of a small lesion for microscopic study 2. Incisional Biopsy - a small section of a large lesion removed for microscopic study www.indiandentalacademy.com
  • 4. Contraindications There are no absolute contraindications for biopsy. The relative contraindication for biopsy are ◦ Pigmented lesions ◦ Vascular lesions ◦ Uncontrolled diabetes ◦ Lesions prone for infections www.indiandentalacademy.com
  • 5. Methods of Biopsy 1. Surgical excision by scalpel 2. Surgical removal by cautery or a high frequency cutting knife 3. Removal by biopsy forceps or biopsy punch 4. Aspiration through a needle with a large lumen 5. Exfoliative cytology www.indiandentalacademy.com
  • 6. Incisional Biopsy Biopsy that samples only a particular portion or representative part of a lesion. If a lesion is large or has different characteristics in various locations more than one area may need to be sampled. www.indiandentalacademy.com
  • 7. Indications:  Indicated in larger lesions for diagnosis (i.e. larger than 1cm in diameter).  Lesions in hazardous location.  Indicated for tumors of the soft tissues to distinguish benign conditions from malignant. www.indiandentalacademy.com
  • 8. Erythroplakia Nodular Leukoplakia Squamous cell carcinoma www.indiandentalacademy.com
  • 10. EXCISIONAL BIOPSYEXCISIONAL BIOPSY Total excision of a small lesion for microscopic study is called excisional biopsy.  It is preferred if the size of the lesion is small enough (<1cm), such that it may be removed along with the margins of normal tissue and wound can be closed primarily. www.indiandentalacademy.com
  • 11. Indications: Lesions smaller than 1 cm. If excision does not risk any vital structure. If clinical impression is that of benign lesion. If excision can be curative. www.indiandentalacademy.com
  • 13. Exfoliative cytologyExfoliative cytology It is the study of cells which exfoliate from the body surfaces www.indiandentalacademy.com
  • 14. Methodology Surface of the lesion is wiped with sponge material which is then sectioned, or scraped. Scrape the surface of lesion vigorously with moistened tongue blade or cytobrush, smeared on a microscopic slide and fixed with 90% alcohol. www.indiandentalacademy.com
  • 15. It is studied by the cytopathologist for presence of atypical or diagnostic cells  The cytopathologist may classify the smear into following classes www.indiandentalacademy.com
  • 16. Class I – Normal – normal cells are present Class II – Atypical – minor atypia but no evidence of malignant changes Class III – intermediate- wider atypia that suggest cancer, but is not clear cut and represent precancerous lesions- ca in situ- Biopsy is recommended www.indiandentalacademy.com
  • 17. Class IV - suggestive of cancer- few cells with malignant characteristics or with many cells with borderline characteristics- Biopsy is mandatory Class V - positive of cancer cells are obviously malignant- Biopsy is mandatory www.indiandentalacademy.com
  • 18. PUNCH BIOPSY Removal of a small disk-shaped sample of tissue. Used in difficult to access areas like retromolar trigone, maxillary tuberosity. Indicated in patients with trismus. www.indiandentalacademy.com
  • 20. Summary Definition of biopsy, the indications and contra- indications of biopsy. Exfoliative cytology, Punch biopsy. www.indiandentalacademy.com
  • 21. BIBLIOGRAPHYBIBLIOGRAPHY Text book of oral pathology Shafer's, 5 & 6th edition Color Atlas of Oral Diseases Cawson, R. 2nd edition Oral and Maxillofacial Pathology Neville, Brad W. 2nd Lucas’s Pathology Of Tumor’s of the Oral Tissues Cawson, R. A., Bennie, W. H 5th edition www.indiandentalacademy.com