Sulfur is an essential nutrient that is incorporated into amino acids like cysteine and methionine. Sulfate is absorbed by plant roots and transported to leaves, where it undergoes a multi-step reduction process to form cysteine. First, sulfate is activated by ATP and converted to APS. APS is then reduced to sulfite and sulfide using electrons from glutathione and ferredoxin. Sulfide reacts with O-acetylserine to form cysteine. Cysteine can then be used to synthesize other sulfur-containing compounds like methionine and the antioxidant glutathione, which transports sulfur within the plant.
Sulphate assimilation which takes place mainly in chloroplasts in higher plants leads to the formation of cysteine. cysteine is the central compound in sulphur assimilation.
Methionine is an amino acid( a building block of protein) hence cannot be produced by the body but only supplied by the diet.Although mammals cannot synthesize methionine, they can still use it in a variety of biochemical pathways: Metabolism,Regeneration, Reverse-transulfurylation pathway: conversion to cysteine & Ethylene synthesis.
ONE OF THESE ABOVE , ONLY METABOLISM IS DISCUSSED.
As an essential amino acid, methionine is not synthesized de novo in humans and other animals, which must ingest methionine or methionine-containing proteins. In plants and microorganisms, methionine biosynthesis belongs to the aspartate family, along with threonine and lysine (via diaminopimelate, but not via α-aminoadipate). The main backbone is derived from aspartic acid, while the sulfur may come from cysteine, methanethiol, or hydrogen sulfide.
Sulphate assimilation which takes place mainly in chloroplasts in higher plants leads to the formation of cysteine. cysteine is the central compound in sulphur assimilation.
Methionine is an amino acid( a building block of protein) hence cannot be produced by the body but only supplied by the diet.Although mammals cannot synthesize methionine, they can still use it in a variety of biochemical pathways: Metabolism,Regeneration, Reverse-transulfurylation pathway: conversion to cysteine & Ethylene synthesis.
ONE OF THESE ABOVE , ONLY METABOLISM IS DISCUSSED.
As an essential amino acid, methionine is not synthesized de novo in humans and other animals, which must ingest methionine or methionine-containing proteins. In plants and microorganisms, methionine biosynthesis belongs to the aspartate family, along with threonine and lysine (via diaminopimelate, but not via α-aminoadipate). The main backbone is derived from aspartic acid, while the sulfur may come from cysteine, methanethiol, or hydrogen sulfide.
Assimilation of ammonium ions is the ultimate aim of nitrogen metabolism in plants. this is the source of nitrogen for various organic compounds of structural and functional importance for the living world
This covers the topic on Proteins, in general. This discusses the different amino acids, bonds formed, structure of proteins and also the different chemical reactions involved with it.
This material is a great help for high school students and students taking up medical and science courses.
Introduction to Aminoacids the basic unit of proteins,there are about 300 amino acids in which 20 occur in proteins, Definition- amino acids are group of organic compounds containing two functional group (amino and carboxyl group) , Explained Classification on the basis of structure polarity metabolic rate and nutrition , Funtions, Structure, Properties and Reference.
Introcution to Proteins, Amino Acids and PolypeptidesDHANANJAY PATIL
A comprehensive introduction to the proteins, amino acids and polypeptides. This will give readers a overall view of this topic. All types of queries and suggestions are most welcome
Methionine metabolism
Activation of methionine and transmethylation
Conversion of methionine to cysteine
Degradation of cysteine.
Cysteine metabolism
Formation
Metabolic Function
Metabolism Disorders of Sulfur containing amino acid
Protein metabolism is more appropriately learnt as metabolism of Amino acid. The proteins on degradation(proteolysis) release individual amino acids. The amount of free amino acids distributed throught the body is called Amino acid pool. The amino acids undergo certain common reactions like transamination followed by deamination for the liberation of ammonia. The amino group of amino acids utilized for the formation of urea, which is the end product of protein metabolism
Assimilation of ammonium ions is the ultimate aim of nitrogen metabolism in plants. this is the source of nitrogen for various organic compounds of structural and functional importance for the living world
This covers the topic on Proteins, in general. This discusses the different amino acids, bonds formed, structure of proteins and also the different chemical reactions involved with it.
This material is a great help for high school students and students taking up medical and science courses.
Introduction to Aminoacids the basic unit of proteins,there are about 300 amino acids in which 20 occur in proteins, Definition- amino acids are group of organic compounds containing two functional group (amino and carboxyl group) , Explained Classification on the basis of structure polarity metabolic rate and nutrition , Funtions, Structure, Properties and Reference.
Introcution to Proteins, Amino Acids and PolypeptidesDHANANJAY PATIL
A comprehensive introduction to the proteins, amino acids and polypeptides. This will give readers a overall view of this topic. All types of queries and suggestions are most welcome
Methionine metabolism
Activation of methionine and transmethylation
Conversion of methionine to cysteine
Degradation of cysteine.
Cysteine metabolism
Formation
Metabolic Function
Metabolism Disorders of Sulfur containing amino acid
Protein metabolism is more appropriately learnt as metabolism of Amino acid. The proteins on degradation(proteolysis) release individual amino acids. The amount of free amino acids distributed throught the body is called Amino acid pool. The amino acids undergo certain common reactions like transamination followed by deamination for the liberation of ammonia. The amino group of amino acids utilized for the formation of urea, which is the end product of protein metabolism
Plants absorb nitrogen from the soil in the form of nitrate (NO3−) and ammonium (NH4+). In aerobic soils where nitrification can occur, nitrate is usually the predominant form of available nitrogen that is absorbed. However this is not always the case as ammonia can predominate in grasslands and in flooded, anaerobic soils like rice paddies.[4] Plant roots themselves can affect the abundance of various forms of nitrogen by changing the pH and secreting organic compounds or oxygen. This influences microbial activities like the inter-conversion of various nitrogen species, the release of ammonia from organic matter in the soil and the fixation of nitrogen by non-nodule-forming bacteria.
Ammonium ions are absorbed by the plant via ammonia transporters. Nitrate is taken up by several nitrate transporters that use a proton gradient to power the transport.Nitrogen is transported from the root to the shoot via the xylem in the form of nitrate, dissolved ammonia and amino acids. Usually (but not always most of the nitrate reduction is carried out in the shoots while the roots reduce only a small fraction of the absorbed nitrate to ammonia. Ammonia (both absorbed and synthesized) is incorporated into amino acids via the glutamine synthetase-glutamate synthase (GS-GOGAT) pathway. While nearly all the ammonia in the root is usually incorporated into amino acids at the root itself, plants may transport significant amounts of ammonium ions in the xylem to be fixed in the shoots.This may help avoid the transport of organic compounds down to the roots just to carry the nitrogen back as amino acids.
Nitrate reduction is carried out in two steps. Nitrate is first reduced to nitrite (NO2−) in the cytosol by nitrate reductase using NADH or NADPH. Nitrite is then reduced to ammonia in the chloroplasts (plastids in roots) by a ferredoxin dependent nitrite reductase. In photosynthesizing tissues, it uses an isoform of ferredoxin (Fd1) that is reduced by PSI while in the root it uses a form of ferredoxin (Fd3) that has a less negative midpoint potential and can be reduced easily by NADPH. In non photosynthesizing tissues, NADPH is generated by glycolysis and the pentose phosphate pathway.
In the chloroplasts,glutamine synthetase incorporates this ammonia as the amide group of glutamine using glutamate as a substrate. Glutamate synthase (Fd-GOGAT and NADH-GOGAT) transfer the amide group onto a 2-oxoglutarate molecule producing two glutamates. Further transaminations are carried out make other amino acids (most commonly asparagine) from glutamine. While the enzyme glutamate dehydrogenase (GDH) does not play a direct role in the assimilation, it protects the mitochondrial functions during periods of high nitrogen metabolism and takes part in nitrogen remobilization.
Photosynthesis is an inevitable process that keeps us alive.It is the main source for food and it's byproduct keeps us breathing. This ppt is the detailed explanation of photosynthesis and the components involved in it. Here you can easily understand the concept and you are able to strengthen your grip on this topic.
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
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Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
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Safalta Digital marketing institute in Noida, provide complete applications that encompass a huge range of virtual advertising and marketing additives, which includes search engine optimization, virtual communication advertising, pay-per-click on marketing, content material advertising, internet analytics, and greater. These university courses are designed for students who possess a comprehensive understanding of virtual marketing strategies and attributes.Safalta Digital Marketing Institute in Noida is a first choice for young individuals or students who are looking to start their careers in the field of digital advertising. The institute gives specialized courses designed and certification.
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Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
2. SULPHUR
Sulfur is an essential macronutrient required for plant
growth. It is primarily used to synthesize cysteine,
methionine and numerous essential and secondary
metabolites derived from these amino acids.
Disulfide bridges in proteins play structural and
regulatory roles.
Sulfur participates in electron transport through iron–
sulfur clusters.
The catalytic sites for several enzymes and
coenzymes, such as urease and coenzyme A, contain
sulfur.
3. Secondary metabolites that contain sulfur range from
the rhizobial Nod factors antiseptic alliin in garlic and
anticarcinogen sulforaphane in broccoli.
Sulfate enters a plant primarily through the roots by
way of an active uptake mechanism.
Gaseous sulfur dioxide readily enters the leaves,
where it is assimilated.
4. SULPHATE ASSIMILATION
Conversion of inorganic sulphur compounds
such as SO4
2- into sulfur-containing organic
compounds such as cysteine by plants is called
as sulfur or sulfate assimilation
Sulfate assimilation takes place chiefly in leaves
in chloroplasts. The sulfate absorbed by roots
from soil solution is translocated through xylem
to shoots for assimilation. To some extent sulfate
assimilation may also occur in roots in
proplastids
5. Sulfur in sulfate is present in highly oxidised state
with six positive charges while in cysteine it is present
in reduced state with four negative charges.
Therefore, conversion of sulfate into cysteine is a
reduction process that is energy dependent and
requires ATP.
It entails transfer of 10 electrons which are provided
by reduced glutathione (GSH), reduced ferredoxin
(Fd. red) and O-Acetylserine
9. STEPS INVOLVED
In the first step, sulfate is activated by ATP in the
presence of the enzyme ATP- sulfurylase to form
APS (Adenosine-5′-phosphosulfate) and
pyrophosphate (PPi). Mg++ ions are required in this
reaction
SO4
2- + Mg-ATP → APS + PPi
PPi so formed is quickly hydrolysed by the enzyme
inorganic pyrophosphatase to yield two molecules of
inorganic phosphate (2Pi).
PPi + H2O → 2Pi
10. The APS is now reduced . The enzyme APS-
reductase transfers two electrons from two molecules
of reduced glutathione (2 GSH) to produce sulfite
(SO3
2-). Glutathione is oxidised (GSSG).
APS + 2 GSH → SO3
2- + 2 H+ + GSSG + AMP
11. Sulfite (SO3
2-) is now reduced to form sulfide (S2-) in
the presence of the enzyme sulfite reductase. This
reduction requires six electrons which are provided by
6 mols of reduced ferredoxin (Fd.red).
SO3
2- + 6 Fd.red → S2- + 6 Fd.oxi
12. In the last step, sulfide reacts with O-acetylserine
(OAS) under the influence of the enzyme OAS-
thiolyase to form the amino acid cysteine and acetic
acid. There is transfer of two electrons in this
reduction, from OAS to sulfide.
OAS + S2- → cysteine + acetate
(The OAS is formed from serine and acetyl-CoA in
the presence of the enzyme serine acetyl transferase.)
13. Formation of other sulphur containing
compounds
After the synthesis of cysteine, another sulfur-
containing amino acid methionine is synthesized from
it.
Thereafter, other sulfur-containing organic
compounds are synthesized from these two amino
acids.
15. Various steps of synthesis of methionine
from cysteine are as follows:
(i) In the first step, cysteine reacts with O-
phosphohomoserine in the presence of the enzyme
cystathionine synthase to form cystathionine.
Inorganic phosphate (Pi) is released in the reaction.
(ii) In the next step, the enzyme cystathionine β-Iyase
splits cystathionine into homocysteine, NH3 and
pyruvate.
iii) Finally, homocysteine is methylated by the
enzyme methionine synthase to form methionine.
16. Glutathione
GSH (y-glutamyl-cysteinyl-glycine) and GSSH,
reduced and oxidized forms of glutathione,
respectively, are readily interchangeable.
This tripeptide (y -Glu-Cys-Gly) is the dominant non-
protein thiol in plants and can play a role in regulating
the uptake of So4 by plant roots.
Glutathione accumulates after excess feeding of sulfur
compounds if the normal regulatory control
mechanisms are circumvented , suggesting that
glutathione functions as a storage pool for excess
cysteine.
18. BIOSYNTHESIS OF GLUTATHIONE
GSH is synthesized by a γ-glutamyl-cysteine
synthase and has been characterized from Nicotiana
tabacum .
This compound is condensed with glycine by the
glutathione synthase, forming GSH.
19.
20. The sulfur-containing organic compounds produced
after sulfur (sulfate) assimilation are exported to
other parts of the plant, such as root and shoot
apices and fruits, through phloem chiefly as
glutathione.
It is believed glutathione also contribute absorption
of sulphate by roots and its assimilation by shoots.