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Bilingual notes of Pharmacognosy chapter 4Glycosides, Volatile oils,Tannins,Resins.pdf
1. PHARMACOGNOSY
CHAPTER 4
1. Glycosides
тЧП In a glycoside molecules, sugar group is bonded through its anomeric carbon to another
group via glcosidic bond. A glycosidic bond is a covalent bond that binds the sugar
molecule to another molecule (that may or may not be sugar molucles.) Thus a
sunstance containing a glycosidic bond is called a glycoside.
рдЧреНрд▓рд╛рдЗрдХреЛрд╕рд╛рдЗрдб рдЕрдгреБрдУрдВ рдореЗрдВ, рдЪреАрдиреА рд╕рдореВрд╣ рдЕрдкрдиреЗ рдПрдиреЛрдореЗрд░рд┐рдХ рдХрд╛рд░реНрдмрди рдХ
реЗ рдорд╛рдзреНрдпрдо рд╕реЗ рдЧреНрд▓рд╛рдЗрдХреЛрд╕рд┐рдбрд┐рдХ рдмрдВрдзрди рдХ
реЗ
рдорд╛рдзреНрдпрдо рд╕реЗ рджреВрд╕рд░реЗ рд╕рдореВрд╣ рд╕реЗ рдмрдВрдзрд╛ рд╣реЛрддрд╛ рд╣реИред рдЧреНрд▓рд╛рдЗрдХреЛрд╕рд┐рдбрд┐рдХ рдмрдВрдзрди рдПрдХ рд╕рд╣рд╕рдВрдпреЛрдЬрдХ рдмрдВрдзрди рд╣реЛрддрд╛ рд╣реИ рдЬреЛ рдЪреАрдиреА
рдЕрдгреБ рдХреЛ рджреВрд╕рд░реЗ рдЕрдгреБ рд╕реЗ рдмрд╛рдВрдзрддрд╛ рд╣реИ (рдЬреЛ рдЪреАрдиреА рдЕрдгреБ рд╣реЛ рднреА рд╕рдХрддрд╛ рд╣реИ рдФрд░ рдирд╣реАрдВ рднреА)ред рдЗрд╕ рдкреНрд░рдХрд╛рд░ рдПрдХ рд╕рдирд╕реНрдЯрд╛рдВрд╕
рдпреБрдХреНрдд рд╣реЛрддрд╛ рд╣реИред рдЧреНрд▓рд╛рдЗрдХреЛрд╕рд┐рдбрд┐рдХ рдмрдВрдзрди рдХреЛ рдЧреНрд▓рд╛рдЗрдХреЛрд╕рд╛рдЗрдб рдХрд╣рд╛ рдЬрд╛рддрд╛ рд╣реИ
тЧП Glycosidic may be defined as "the organic compounds of plants or animals origin which
on enzymatic or acid hydrolysis give one or more sugar moieyics along with non-sugar
moiety.
рдЧреНрд▓рд╛рдЗрдХреЛрд╕рд┐рдбрд┐рдХ рдХреЛ "рдкреМрдзреЛрдВ рдпрд╛ рдЬрд╛рдирд╡рд░реЛрдВ рдХ
реЗ рдХрд╛рд░реНрдмрдирд┐рдХ рдпреМрдЧрд┐рдХреЛрдВ рдХ
реЗ рд░реВрдк рдореЗрдВ рдкрд░рд┐рднрд╛рд╖рд┐рдд рдХрд┐рдпрд╛ рдЬрд╛ рд╕рдХрддрд╛ рд╣реИ рдЬреЛ
рдПрдВрдЬрд╛рдЗрдореИрдЯрд┐рдХ рдпрд╛ рдПрд╕рд┐рдб рд╣рд╛рдЗрдбреНрд░реЛрд▓рд┐рд╕рд┐рд╕ рдкрд░ рдЧреИрд░-рд╢рд░реНрдХ рд░рд╛ рдЕрдВрд╢ рдХ
реЗ рд╕рд╛рде рдПрдХ рдпрд╛ рдЕрдзрд┐рдХ рдЪреАрдиреА рдЕрдВрд╢ рджреЗрддреЗ рд╣реИрдВтАЭ
тЧП The sugar group is known as the glycone and the non-sugar group as aglycone or genin
part of the glycoside.
рд╢рд░реНрдХ рд░рд╛ рд╕рдореВрд╣ рдХреЛ рдЧреНрд▓рд╛рдЗрдХреЛрди рдХ
реЗ рд░реВрдк рдореЗрдВ рдЬрд╛рдирд╛ рдЬрд╛рддрд╛ рд╣реИ рдФрд░ рдЧреИрд░-рд╢рд░реНрдХ рд░рд╛ рд╕рдореВрд╣ рдХреЛ рдПрдЧреНрд▓реАрдХреЛрди рдпрд╛ рдЧреНрд▓рд╛рдЗрдХреЛрд╕рд╛рдЗрдб
рдХ
реЗ рдЬреЗрдирд┐рди рднрд╛рдЧ рдХ
реЗ рд░реВрдк рдореЗрдВ рдЬрд╛рдирд╛ рдЬрд╛рддрд╛ рд╣реИред
Occurance and Distribution
тЧП Glycosides widely occurs in root, bark, fruits, and to a small extent in leaves.
рдЧреНрд▓рд╛рдЗрдХреЛрд╕рд╛рдЗрдб рд╡реНрдпрд╛рдкрдХ рд░реВрдк рд╕реЗ рдЬрдбрд╝, рдЫрд╛рд▓, рдлрд▓реЛрдВ рдФрд░ рдХ
реБ рдЫ рд╣рдж рддрдХ рдкрддреНрддрд┐рдпреЛрдВ рдореЗрдВ рдкрд╛рдпрд╛ рдЬрд╛рддрд╛ рд╣реИ
Isolation
. Sta-Otta method
Identification Tests
Borntragers Test: 1 gm of drug is boiled with 5-10 ml dilute HCL for 10 Min on a water bath.
тЧП After boiling the solution is filtered and the filtrate is extracted with CCl4 / benzene.
тЧП The obtained extracted solution is shaken with an equal quantity of ammonia solution. A
pink or red colour Appears in the ammonical layer Which confirms the presence of
anthraquinone moiety.
1 рдЧреНрд░рд╛рдо рджрд╡рд╛ рдХреЛ 5-10 рдорд┐рд▓реАрд▓реАрдЯрд░ рдкрддрд▓рд╛ рдПрдЪрд╕реАрдПрд▓ рдХ
реЗ рд╕рд╛рде рдкрд╛рдиреА рдХ
реЗ рд╕реНрдирд╛рди рдореЗрдВ 10 рдорд┐рдирдЯ рддрдХ рдЙрдмрд╛рд▓рд╛ рдЬрд╛рддрд╛
рд╣реИред рдЙрдмрд▓рдиреЗ рдХ
реЗ рдмрд╛рдж рдШреЛрд▓ рдХреЛ рдЫрд╛рди рд▓рд┐рдпрд╛ рдЬрд╛рддрд╛ рд╣реИ рдФрд░ рдЫрд╛рдирдХрд░ CCl4/рдмреЗрдВрдЬреАрди рдХ
реЗ рд╕рд╛рде рдирд┐рдХрд╛рд▓ рд▓рд┐рдпрд╛ рдЬрд╛рддрд╛ рд╣реИред
NOTES BY SUMIT TIWARI
2. PHARMACOGNOSY
CHAPTER 4
рдкреНрд░рд╛рдкреНрдд рдирд┐рдХрд╛рд▓реЗ рдЧрдП рдШреЛрд▓ рдХреЛ рд╕рдорд╛рди рдорд╛рддреНрд░рд╛ рдореЗрдВ рдЕрдореЛрдирд┐рдпрд╛ рдШреЛрд▓ рдХ
реЗ рд╕рд╛рде рд╣рд┐рд▓рд╛рдпрд╛ рдЬрд╛рддрд╛ рд╣реИред рдЕрдореЛрдирд┐рдХрд▓ рдкрд░рдд рдореЗрдВ
рдПрдХ рдЧреБрд▓рд╛рдмреА рдпрд╛ рд▓рд╛рд▓ рд░рдВрдЧ рджрд┐рдЦрд╛рдИ рджреЗрддрд╛ рд╣реИ рдЬреЛ рдПрдиреНрдереНрд░рд╛рдХреНрд╡рд┐рдиреЛрди рднрд╛рдЧ рдХреА рдЙрдкрд╕реНрдерд┐рддрд┐ рдХреА рдкреБрд╖реНрдЯрд┐ рдХрд░рддрд╛ рд╣реИред
Therapeutics Activity
Cardiac glycosides are compounds derived from the foxglove plant (Digitalis purpurea). Initially,
digitalis was used to treat dropsy (i.e., oedema). Later studies revealed that digitalis may treat
oedema caused by a weakened heart (i.e., heart failure).
рдХрд╛рд░реНрдбрд┐рдпрдХ рдЧреНрд▓рд╛рдЗрдХреЛрд╕рд╛рдЗрдбреНрд╕ рдлреЙрдХреНрд╕рдЧреНрд▓реЛрд╡ рдкреМрдзреЗ (рдбрд┐рдЬрд┐рдЯрд▓рд┐рд╕ рдкреБрд░рдкреБрд░рд┐рдпрд╛) рд╕реЗ рдкреНрд░рд╛рдкреНрдд рдпреМрдЧрд┐рдХ рд╣реИрдВред рдкреНрд░рд╛рд░рдВрдн рдореЗрдВ, рдбрд┐рдЬрд┐рдЯрд▓рд┐рд╕ рдХрд╛
рдЙрдкрдпреЛрдЧ рдбреНрд░реЙрдкреНрд╕реА (рдпрд╛рдиреА, рдПрдбрд┐рдорд╛) рдХ
реЗ рдЗрд▓рд╛рдЬ рдХ
реЗ рд▓рд┐рдП рдХрд┐рдпрд╛ рдЬрд╛рддрд╛ рдерд╛ред рдмрд╛рдж рдХ
реЗ рдЕрдзреНрдпрдпрдиреЛрдВ рд╕реЗ рдкрддрд╛ рдЪрд▓рд╛ рдХрд┐ рдбрд┐рдЬрд┐рдЯрд▓рд┐рд╕
рдХрдордЬреЛрд░ рджрд┐рд▓ (рдпрд╛рдиреА, рджрд┐рд▓ рдХреА рд╡рд┐рдлрд▓рддрд╛) рдХ
реЗ рдХрд╛рд░рдг рд╣реЛрдиреЗ рд╡рд╛рд▓реА рд╕реВрдЬрди рдХрд╛ рдЗрд▓рд╛рдЬ рдХрд░ рд╕рдХрддрд╛ рд╣реИред
2. Volatile Oils
тЧП Volatile oils are obtained from various plant parts. They are basically the mixture of
hydrocarbon sesquiterpenes, and polyterpenes and their oxygenated derivatives.
рд╡рд╛рд╖реНрдкрд╢реАрд▓ рддреЗрд▓ рд╡рд┐рднрд┐рдиреНрди рдкреМрдзреЛрдВ рдХ
реЗ рднрд╛рдЧреЛрдВ рд╕реЗ рдкреНрд░рд╛рдкреНрдд рд╣реЛрддреЗ рд╣реИрдВред рд╡реЗ рдореВрд▓ рд░реВрдк рд╕реЗ рд╣рд╛рдЗрдбреНрд░реЛрдХрд╛рд░реНрдмрди рд╕реЗрд╕реНрдХреНрдпреВрдЯрд░рдкреАрди, рдФрд░
рдкреЙрд▓реАрдЯрд░рдкреАрди рдФрд░ рдЙрдирдХ
реЗ рдСрдХреНрд╕реАрдЬрдирдпреБрдХреНрдд рдбреЗрд░рд┐рд╡реЗрдЯрд┐рд╡ рдХрд╛ рдорд┐рд╢реНрд░рдг рд╣реИрдВред
тЧП Volatile oils carry the plant's active constituents and hence are also called essential oils.
Mostly the volatile oils are already present in special secretory tissues in the Plant, e.g.,
the oil ducts in umbelliferous fruits.
рд╡рд╛рд╖реНрдкрд╢реАрд▓ рддреЗрд▓реЛрдВ рдореЗрдВ рдкреМрдзреЗ рдХ
реЗ рд╕рдХреНрд░рд┐рдп рдШрдЯрдХ рд╣реЛрддреЗ рд╣реИрдВ рдФрд░ рдЗрд╕рд▓рд┐рдП рдЙрдиреНрд╣реЗрдВ рдЖрд╡рд╢реНрдпрдХ рддреЗрд▓ рднреА рдХрд╣рд╛ рдЬрд╛рддрд╛ рд╣реИред
рдЕрдзрд┐рдХрддрд░ рд╡рд╛рд╖реНрдкрд╢реАрд▓ рддреЗрд▓ рдкрд╣рд▓реЗ рд╕реЗ рд╣реА рдкреМрдзреЗ рдХ
реЗ рд╡рд┐рд╢реЗрд╖ рд╕реНрд░рд╛рд╡реА рдКрддрдХреЛрдВ рдореЗрдВ рдореМрдЬреВрдж рд╣реЛрддреЗ рд╣реИрдВ, рдЙрджрд╛рд╣рд░рдг рдХ
реЗ рд▓рд┐рдП,
рдирд╛рднрд┐рджрд╛рд░ рдлрд▓реЛрдВ рдореЗрдВ рддреЗрд▓ рдирд▓рд┐рдХрд╛рдПрдВред
тЧП Volatile or ethereal oils are defined principles of plant as, "odorous volatile principle of
plants and animal origin which evaporate when exposed to air at ordinary temperature"
рд╡рд╛рд╖реНрдкрд╢реАрд▓ рдпрд╛ рдИрдерд░ рддреЗрд▓реЛрдВ рдХреЛ рдкреМрдзреЗ рдХ
реЗ рд╕рд┐рджреНрдзрд╛рдВрддреЛрдВ рдХ
реЗ рд░реВрдк рдореЗрдВ рдкрд░рд┐рднрд╛рд╖рд┐рдд рдХрд┐рдпрд╛ рдЧрдпрд╛ рд╣реИ, "рдкреМрдзреЛрдВ рдФрд░ рдЬрд╛рдирд╡рд░реЛрдВ рдХреА
рдЙрддреНрдкрддреНрддрд┐ рдХрд╛ рдЧрдВрдзрдпреБрдХреНрдд рдЕрд╕реНрдерд┐рд░ рд╕рд┐рджреНрдзрд╛рдВрдд рдЬреЛ рд╕рд╛рдорд╛рдиреНрдп рддрд╛рдкрдорд╛рди рдкрд░ рд╣рд╡рд╛ рдХ
реЗ рд╕рдВрдкрд░реНрдХ рдореЗрдВ рдЖрдиреЗ рдкрд░ рд╡рд╛рд╖реНрдкрд┐рдд рд╣реЛ
рдЬрд╛рддрд╛ рд╣реИтАЭ
Occurrence and Distribution
тЧП Aromatic plants contain essential oils
тЧП Volatile oils can be formed by two methods, i.e., by hydrolysis of some glycosides, and
directly by the Protoplasm
NOTES BY SUMIT TIWARI
3. PHARMACOGNOSY
CHAPTER 4
Isolation
тЧП The amount of volatile oils present in plants reaches its maximum at some particular
time, e.g.. jasmine has the greatest concentration of volatile oil at sunset, and thus the
plant parts containing volatile oils should be collected at this particular time.
тЧП Extraction by Steam Distillation:
тЧП Extraction by Scarification:
Identification Tests
1) A thin section of drug is treated with an alcoholic solution of Sudan III. A red colour appears
that confirms the presence of volatile oils.
2) A thin section of drug is treated with a tincture of alkane. A red colour appears that confirms
the presence of volatile oils.
Therapeutic Activity
volatile oil molecules enter the body, they correlate with physiological functions through the
following three modes of action:
A. Biochemical (Pharmacological): They chemically interact in the bloodstream with
hormones and enzymes (like farnesene).
B. Physiological: They interact by participating in specific physiological function; For example,
the volatile oil present in fennel contains oestrogen-like compounds which prevent female
problems (like lactation and menstruation).
C. Psychological: On inhaling the volatile oil molecules, the factory area of the brain (limbic
system) gets triggered and releases chemical and neurotransmitter messengers.
Pharmaceutical Applications
Volatile oils are employed as flavouring agent, perfuming agent in pharmaceutical products,
foods, beverages, and in cosmetics.
NOTES BY SUMIT TIWARI
4. PHARMACOGNOSY
CHAPTER 4
3. Tannins
тЧП The term tannin was first used by Seguin in 1976 to denote substances present in plant
extracts which are able to combine with proteins of animal hides, prevent their
putrefaction, and convert them into leather.
тЧП On this basis tannin is a substance which is detected qualitatively by a tanning test (the
Goldbeater's skin test) and is determined quantitatively by its absorption on standard
hide powder.
тЧП Tannins are defined as, "complex substances that usually Occur as mixtures of
polyphenols that are very difficult to separate since they do not crystallise".
рдЯреИрдирд┐рди рдХреЛ рдЗрд╕ рдкреНрд░рдХрд╛рд░ рдкрд░рд┐рднрд╛рд╖рд┐рдд рдХрд┐рдпрд╛ рдЧрдпрд╛ рд╣реИ, "рдЬрдЯрд┐рд▓ рдкрджрд╛рд░реНрде рдЬреЛ рдЖрдорддреМрд░ рдкрд░ рдкреЙрд▓реАрдл
реЗ рдиреЛрд▓реНрд╕ рдХ
реЗ рдорд┐рд╢реНрд░рдг рдХ
реЗ
рд░реВрдк рдореЗрдВ рд╣реЛрддреЗ рд╣реИрдВ рдЬрд┐рдиреНрд╣реЗрдВ рдЕрд▓рдЧ рдХрд░рдирд╛ рдмрд╣реБрдд рдореБрд╢реНрдХрд┐рд▓ рд╣реЛрддрд╛ рд╣реИ рдХреНрдпреЛрдВрдХрд┐ рд╡реЗ рдХреНрд░рд┐рд╕реНрдЯрд▓реАрдХреГ рдд рдирд╣реАрдВ рд╣реЛрддреЗ рд╣реИрдВ"ред
тЧП Tannins are present in the aerial parts, e.g., leaves, fruits, barks, or stem, generally
occurs in immature fruits, but disappears during the ripening process.
рдЯреИрдирд┐рди рд╣рд╡рд╛рдИ рднрд╛рдЧреЛрдВ рдореЗрдВ рдореМрдЬреВрдж рд╣реЛрддреЗ рд╣реИрдВ, рдЬреИрд╕реЗ, рдкрддреНрддрд┐рдпрд╛рдВ, рдлрд▓, рдЫрд╛рд▓ рдпрд╛ рддрдиреЗ, рдЖрдорддреМрд░ рдкрд░ рдЕрдкрд░рд┐рдкрдХреНрд╡ рдлрд▓реЛрдВ рдореЗрдВ
рд╣реЛрддреЗ рд╣реИрдВ, рд▓реЗрдХрд┐рди рдкрдХрдиреЗ рдХреА рдкреНрд░рдХреНрд░рд┐рдпрд╛ рдХ
реЗ рджреМрд░рд╛рди рдЧрд╛рдпрдм рд╣реЛ рдЬрд╛рддреЗ рд╣реИрдВред
Occurrence and Distribution
тЦ▓ Rhubarb, red rose petals, bearberry leaves, Chinese galls, Turkish gall, hamamelis, chestnut,
and maple are rich in gallitannins. Pomegranate rind, pomegranate myrobolans, eucalyptus
leaves, some Australian kinds and Oak bark are rich in ellagitannins
тЦ▓ parts of plants are rich in condensed tannins
1) Barks: Cinnamon, wild cherry, cinchona, willow, acacia, oak, and hamamelis.
2) Roots and Rhizomes: Krameria and male fern.
3) Flowers: Lime and hawthorn.
4) Seeds: Cocoa, guarana, kola, and areca.
5) Leaves: Hamamelis, howathron, and tea.
6) Extracts and Dried Juice: Catechu, acacia, mangrove cutches, East Indian Kino, butea gum,
eucalyptus, and kino.
NOTES BY SUMIT TIWARI
5. PHARMACOGNOSY
CHAPTER 4
Isolation
The steps involved in extracting tannins are:
1) 100gm of the powdered sample is refluxed for 15 minutes in a 500ml Erlenmeyer flask.
During the reflux process, the entire sample should be covered with 70% acetone. The flask is
removed from the hot plate and filtered in another flask.
2) The residue on the filter paper is washed off using a sufficient amount of 70% acetone.
3) The washed solution is combined with the filtrate and the resultant mixture is subjected to
extraction using diethyl ether.
4) This process is repeated five times till the diethyl ether and tannin separate out completely as
distinct upper and lower layer, respectively.
5) The tannin layer is separated via separatory funnel. The tannin so collected is evaporated till
a residue, known as the extracted tannin, is obtained which is the final product.
6) The tannin percentage is calculated with the following formula:
% tannin = Wt. of dried tannin / Wt. of the powdered sample x 100%
7) After extracting the tannin, the aqueous and ethereal layers are concentrated, dried, further
iso-lated, and purified using various chromatography techniques.
Identification tests:
Gelatin Test: To a solution of tannin (0.5-1%), aqueous solutions of gelatin (1%) and sodium
chloride (10%) are added. A white buff-coloured precipitate is formed.
Therapeutic Activity
The medicinal uses of tannins are:
I. Anti-Diarrhoeal
II. Anti-Tumour
NOTES BY SUMIT TIWARI
6. PHARMACOGNOSY
CHAPTER 4
III.Antidote
4. Resins
тЧП Resins can be defined as, "the hydrocarbon secretion of various plants (especially the
coniferous trees)".
рд░реЗрдЬрд┐рди рдХреЛ "рд╡рд┐рднрд┐рдиреНрди рдкреМрдзреЛрдВ (рд╡рд┐рд╢реЗрд╖ рд░реВрдк рд╕реЗ рд╢рдВрдХ
реБ рдзрд╛рд░реА рдкреЗрдбрд╝реЛрдВ) рдХ
реЗ рд╣рд╛рдЗрдбреНрд░реЛрдХрд╛рд░реНрдмрди рд╕реНрд░рд╛рд╡" рдХ
реЗ рд░реВрдк рдореЗрдВ рдкрд░рд┐рднрд╛рд╖рд┐рдд рдХрд┐рдпрд╛
рдЬрд╛ рд╕рдХрддрд╛ рд╣реИред
тЧП Apart from this, resins are also used as a raw material for synthesising organic
compounds.
рдЗрд╕рдХ
реЗ рдЕрд▓рд╛рд╡рд╛, рд░реЗрдЬрд┐рди рдХрд╛ рдЙрдкрдпреЛрдЧ рдХрд╛рд░реНрдмрдирд┐рдХ рдпреМрдЧрд┐рдХреЛрдВ рдХ
реЗ рд╕рдВрд╢реНрд▓реЗрд╖рдг рдХ
реЗ рд▓рд┐рдП рдХрдЪреНрдЪреЗ рдорд╛рд▓ рдХ
реЗ рд░реВрдк рдореЗрдВ рднреА рдХрд┐рдпрд╛
рдЬрд╛рддрд╛ рд╣реИ
Occurrence and Distribution
Below are the examples of different plants whose different structures or secretion zone contain
resins:
тЧП Ginger (Zingiber officinale Roscoe) belonging to family Zingiberaceae has resin cells
тЧП Cannabis (Cannabis sativa Linn.) belonging to family- Moraceae contain resins in their
glandular hair.
Identification Tests
i.Colophony resins are The tests recommended for colophony resins are
ii. 100mg of resin powder is dissolved in ioml acetic anhydride. To this solution, few drops of
sulphuric acid is added which results in purple violet colouration of the solution.
Guaiacum Resin: A solution of resin is prepared in ethanol and to this solution ferric chloride is
added. This results in deep blue colouration of the solution.
Therapeutic Activity
тЧП Resins possess mild antiseptic property, hence are used topically in the form of plaster,
ointments, and cerates.
тЧП They are also used for preparing emulsions.
NOTES BY SUMIT TIWARI