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Bacteriophage based vector
1. Lamda phage-
It has a double stranded,linearDNA GENOMEOF 48514 bp.
Presence of 12 basesat the bothend.
These are unpairedbutcomplementarycalledcossites.
Cos site isusedforpackagingDNA intophage heads.
The central regionof the genome causeslyticinfection.
Therefore,infectedgenesare substitutedorreplacedbydesiredgenes.
Cloningcapacityis23 kb.
Fig.Lamda phage
M13 Phage-
It isa filamentousphage,infectE.coli havingFpili.
Genome issingle strandedcircular,6407bp insize.
Single strandedgenomeentersintobacteriafromdouble strandardmoleculecalledReplicative form.
Afterforming100 copiesina cell at thatpointreplicationbecome asymmetricandsingle stranded
copiesare produced.
These are extractedoutfromthe hostcelsas M13 PHAGE ANDDOUBLE STRANDEDreplicative form.
Application-
It isusedin site-directedmutagenesisandDNA sequencing.
