Single Cell Protein

1. PRESENTED BY:-
Sonika
MSc

2. CONTENTS
 INTRODUCTION
 SINGLE CELL PROTEIN
 MICROORGANISMS
 BASIC STEPS OF SCP PRODUCTION
 ALGAE AS SCP
 BACTERIA AS SCP
 FUNGAL SCP
 YEAST SCP

3. INTRODUCTION
 Single cell proteins is the dried biomass of
a single species of microbe that can be
used as a protein source in the diet, is
known as single cell protein.
 Biomass of certain algae, some fungi and
some bacteria are used as single cell
proteins.

5. MICROORGANISMS USED IN SCP PRODUCTION 1. ALGAE
 2.BACTERIA
 3. YEAST
 4. FUNGI

6. MICROORGANISMS USED IN SCP PRODUCTION
MUST BE 1. NON PATHOGENIC
 2. GOOD NUTRITIONAL VALUE
 3. EASILY AND CHEAPLY PRODUCED ON SCALE
 4. TOXIN FREE
 5. FAST GROWING
 6. EASY TO SEPARATE FROM MEDIUM

7. MICROORGANISMS
ALGAE BACTERIA FUNGI YEAST

9. ALGAE AS SCP Microalgae such as Spirulina, Chlorella,
Scenedesmus, Dunaliella etc are
consumed as Single Cell Protein.
 Algal SCP has about 60% crude protein.
 They are suitable for animal feeds or
protein rich supplements.

10. SPIRULINA
 Spirulina is a spirally
coiled, multicellular,
filamentous blue
green algae.
 Found in freshwater.
Brackishwater,
marine waters.
 Biomass is a rich
source of protein,
vitamins, minerals
and beta carotene

11. MASS CULTURE OF SPIRULINA
 Cultured in large scales in artificial ponds,
tanks, oxidation ponds.
Three methods of
culturing
 1.Open circulatory system.
 2.oxidation pond system.
 3.photobioreactors(PBR).

12. 1. OPEN CIRCULATORY SYSTEM
 Man-made open tank.
 May be circular or rectangular.
 Medium is filled in the open circulatory
tank.
 Tank is inoculated with small volume of
Spirulina.
 Culture is stirred continuously
 Spirulina grow rapidly.

13. 2.OXIDATION POND SYSTEM
 All solid wastes and suspended particles
are removed from the sewage.
 Sewage water allowed to flow in to an
oxidation pond.
 Spirulina culture is inoculated.
 Spirulina produce dense mat on the
surface of sewage water.
 Biomass is harvested.

14. 3. PHOTOBIOREACTORS(PBRs) These are transparent tubes or
containers.
 PBRs provide more controlled
environment for better growth.
 PBRs offers the following advantages:-
 Easy maintenance of the algal culture.
 Protection from contaminations.
 Avoidance of water loss.
 Low cost of production.

15. USES
As health food.
 As therapeutic agent.
As a supplement animal feed.
As a raw material for cosmetics.
USES

16. BACTERIA
 Dried biomass of some bacteria is rich in
protein.
 They can be grown in cheap renewable
raw material and wastes.
 For ex:- Candida utilis,
 Candida lipolytica,
 Methylophilus methylotrophus,
 Cellulomonas etc are consumed as protein
sources.

17. MASS PRODUCTION OF BACTERIAL SCP 1.FORMULATION OF MEDIUM:-
 Bacterial cells require a suitable carbon
source, P,K, Na,H2 and trace element for their
growth.
 Other nutrients are added to it.
 And dilute properly to formulate culture medium.

18. CONTI….2. FERMENTATION:-
sterilized medium pumped in to air lift
fermenter.
Inoculation of bacterium.
Mixing of sterile air.
Broth is harvested.

19. RECOVERY OF BACTERIAL BIOMASS
+
Centrifuge
HARVESTED
BROTH
WATER
REMOVAL
OF WATER

20. FUNGI AS SCP A few yeast or moulds are being used as
SCP.
 Fungi grow rapidly on wide variety of
cheap raw materials.
 Less amount of nucleic acid.
 Digestibility and acceptability of fungal
protein is high.
FUNGAL AS SCP

21. YEASTS
 Many yeast are consumed as SCP.
 Ex. Saccharomyces fragilis,
 Candida utilis,
 Candida lipolytica,
 Rodotorula glutinis,
 Torulopsis.

22. MASS CULTURE Can be grown by fed batch fermentation.
 Addition of inorganic materials such as
N,P,K,Fe,Mg,AND S.
 Diluted with water and sterilization of
medium.
 Medium is filled in fermenter.
 Inoculation of yeast.
 Sterile air is pumped.
 Broth is harvested.

23. Steri
lized
medi
um
Bacte
rial
suspe
nsion
Centrif
ugatio
n
Drying
Heat
shock
Toxicity
test
SCPferment
er
waterwate
r
Production of bacterial biomass for single cell
protein

24. Reference
Google
Research paper