Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

Cloning Vector

69,843 views

Published on

Published in: Education, Technology, Spiritual
  • DOWNLOAD FULL BOOKS, INTO AVAILABLE FORMAT ......................................................................................................................... ......................................................................................................................... 1.DOWNLOAD FULL. PDF EBOOK here { https://tinyurl.com/yxufevpm } ......................................................................................................................... 1.DOWNLOAD FULL. EPUB Ebook here { https://tinyurl.com/yxufevpm } ......................................................................................................................... 1.DOWNLOAD FULL. doc Ebook here { https://tinyurl.com/yxufevpm } ......................................................................................................................... 1.DOWNLOAD FULL. PDF EBOOK here { https://tinyurl.com/yxufevpm } ......................................................................................................................... 1.DOWNLOAD FULL. EPUB Ebook here { https://tinyurl.com/yxufevpm } ......................................................................................................................... 1.DOWNLOAD FULL. doc Ebook here { https://tinyurl.com/yxufevpm } ......................................................................................................................... ......................................................................................................................... ......................................................................................................................... .............. Browse by Genre Available eBooks ......................................................................................................................... Art, Biography, Business, Chick Lit, Children's, Christian, Classics, Comics, Contemporary, Cookbooks, Crime, Ebooks, Fantasy, Fiction, Graphic Novels, Historical Fiction, History, Horror, Humor And Comedy, Manga, Memoir, Music, Mystery, Non Fiction, Paranormal, Philosophy, Poetry, Psychology, Religion, Romance, Science, Science Fiction, Self Help, Suspense, Spirituality, Sports, Thriller, Travel, Young Adult,
       Reply 
    Are you sure you want to  Yes  No
    Your message goes here
  • DOWNLOAD FULL BOOKS, INTO AVAILABLE FORMAT ......................................................................................................................... ......................................................................................................................... 1.DOWNLOAD FULL. PDF EBOOK here { https://tinyurl.com/y6a5rkg5 } ......................................................................................................................... 1.DOWNLOAD FULL. EPUB Ebook here { https://tinyurl.com/y6a5rkg5 } ......................................................................................................................... 1.DOWNLOAD FULL. doc Ebook here { https://tinyurl.com/y6a5rkg5 } ......................................................................................................................... 1.DOWNLOAD FULL. PDF EBOOK here { https://tinyurl.com/y6a5rkg5 } ......................................................................................................................... 1.DOWNLOAD FULL. EPUB Ebook here { https://tinyurl.com/y6a5rkg5 } ......................................................................................................................... 1.DOWNLOAD FULL. doc Ebook here { https://tinyurl.com/y6a5rkg5 } ......................................................................................................................... ......................................................................................................................... ......................................................................................................................... .............. Browse by Genre Available eBooks ......................................................................................................................... Art, Biography, Business, Chick Lit, Children's, Christian, Classics, Comics, Contemporary, Cookbooks, Crime, Ebooks, Fantasy, Fiction, Graphic Novels, Historical Fiction, History, Horror, Humor And Comedy, Manga, Memoir, Music, Mystery, Non Fiction, Paranormal, Philosophy, Poetry, Psychology, Religion, Romance, Science, Science Fiction, Self Help, Suspense, Spirituality, Sports, Thriller, Travel, Young Adult,
       Reply 
    Are you sure you want to  Yes  No
    Your message goes here
  • DOWNLOAD FULL BOOKS, INTO AVAILABLE FORMAT ......................................................................................................................... ......................................................................................................................... 1.DOWNLOAD FULL. PDF EBOOK here { https://tinyurl.com/yxufevpm } ......................................................................................................................... 1.DOWNLOAD FULL. EPUB Ebook here { https://tinyurl.com/yxufevpm } ......................................................................................................................... 1.DOWNLOAD FULL. doc Ebook here { https://tinyurl.com/yxufevpm } ......................................................................................................................... 1.DOWNLOAD FULL. PDF EBOOK here { https://tinyurl.com/yxufevpm } ......................................................................................................................... 1.DOWNLOAD FULL. EPUB Ebook here { https://tinyurl.com/yxufevpm } ......................................................................................................................... 1.DOWNLOAD FULL. doc Ebook here { https://tinyurl.com/yxufevpm } ......................................................................................................................... ......................................................................................................................... ......................................................................................................................... .............. Browse by Genre Available eBooks ......................................................................................................................... Art, Biography, Business, Chick Lit, Children's, Christian, Classics, Comics, Contemporary, Cookbooks, Crime, Ebooks, Fantasy, Fiction, Graphic Novels, Historical Fiction, History, Horror, Humor And Comedy, Manga, Memoir, Music, Mystery, Non Fiction, Paranormal, Philosophy, Poetry, Psychology, Religion, Romance, Science, Science Fiction, Self Help, Suspense, Spirituality, Sports, Thriller, Travel, Young Adult,
       Reply 
    Are you sure you want to  Yes  No
    Your message goes here
  • I am 31 years old and have had severe Yeast Infection for over 8 years. I have been suffering (yes! really suffering for so long) and I have indeed tried every over-the-counter and prescription that you could think of to treat this horrible disease. My wife also suffered from moderate Yeast Infection for years and we both started your Yeast Infection No More system about 4 months ago and I am so excited to report that we are both free from Yeast Infection. Both of us! For the first time ever. The symptoms are gone...completely! And the overall feeling is unbelievable. Thank you for your help... ♥♥♥ https://tinyurl.com/y4uu6uch
       Reply 
    Are you sure you want to  Yes  No
    Your message goes here
  • STOP GETTING RIPPED OFF! LEARN THE SHOCKING TRUTH ABOUT CANDIDA, YEAST INFECTION, DRUGS AND CREAMS... To get the FACTS on exactly how to eliminate your yeast infection from the root 100% naturally and Permanently and achieve LASTING freedom from candida related symptoms without spending your hard-earned money on drugs and over the counters... ♣♣♣ http://ishbv.com/index7/pdf
       Reply 
    Are you sure you want to  Yes  No
    Your message goes here

Cloning Vector

  1. 1. A SEMINAR ON GENE CLONING VECTORS PRESENTED BY NASREEN SULTAN M.Sc BIOTECHNOLOGY 3 RD SEMESTER
  2. 2. INTRODUCTION <ul><li>A cloning vector is a DNA molecule in which foreign DNA can be inserted or integrated and which is further capable of replicating within host cell to produce multiple clones of recombinant DNA. </li></ul><ul><li>Examples: Plasmids,phage or virus </li></ul>
  3. 3. Characteristics <ul><li>It should be able to replicate autonomously. </li></ul><ul><li>Origin of replication. </li></ul><ul><li>Selectable markers. </li></ul><ul><li>Restriction sites. </li></ul>
  4. 4. Types <ul><li>Plasmid as vector . </li></ul><ul><li>Bacteriophage as vector. </li></ul><ul><li>Cosmid as vector </li></ul><ul><li>Phagemid as vector. </li></ul>
  5. 5. Plasmid Vector: pBR322 <ul><ul><ul><li>Contains: </li></ul></ul></ul><ul><li>Selectable Markers : </li></ul><ul><li>Ampicillin resistance gene. </li></ul><ul><li>Tetracycline resistance gene. </li></ul><ul><li>Col E I replication origin. </li></ul><ul><li>Eco RI site. </li></ul>Structure of E.Coli plasmid cloning vector pBR322 4362 bp
  6. 6. pUC Plasmids <ul><ul><li>Contains </li></ul></ul><ul><ul><li>Ampicillin resistance gene. </li></ul></ul><ul><ul><li>Multiple cloning site. </li></ul></ul><ul><ul><li>ColEI ( origin ). </li></ul></ul>
  7. 7. Multiple Cloning Site (MCS or Polylinker) .
  8. 8. How insertion takes place
  9. 9. Screening procedure of cloning vector <ul><li>Blue/White selection. </li></ul><ul><li>replica plating technique. </li></ul>
  10. 10. Blue/White Selection Only colonies from bacteria that have plasmid IPTG + X-Gal Overnight growth Bacteria with plasmid plus insert Colonies with insert - white Colonies w/o insert - blue
  11. 11. Based on the enzymatic reaction of β -galactosidase
  12. 12. Replica Plating Technique
  13. 13. BACTERIOPHAGE VECTORS <ul><li>It infects bacteria. </li></ul><ul><li>Follow either lytic or lysogenic cycle. </li></ul><ul><li>Lambda phage vector </li></ul><ul><li>high transformation efficiency, about 1000 times more efficient than the plasmid vector. </li></ul><ul><li>Origin of replication. </li></ul><ul><li>genes for head and tail protein. </li></ul><ul><li>single- stranded protruding cohesive ends. </li></ul><ul><li>Size is 48,502 bp. </li></ul>
  14. 14. . DNA cloning using phages as vectors
  15. 15. PHAGE M13 VECTOR
  16. 16. Cosmid vector <ul><li>Combine parts of the lambda chromosome with parts of plasmids. </li></ul><ul><li>an origin of replication (ori). </li></ul><ul><li>a cos site(a sequence yield cohesive end) . </li></ul><ul><li>an ampicillin resistance gene ( amp ), </li></ul><ul><li>restriction sites for cloning </li></ul><ul><li>Cosmids can carry up to 50 kb of inserted DNA. </li></ul>
  17. 17. Cloning by using Cosmid vectors
  18. 18. Latest Generation VECTOR: Phagemid- p Bluescript
  19. 19. APPLICATION <ul><li>A particular gene can be isolated and its nucleotide sequence determined </li></ul><ul><li>Control sequences of DNA can be identified & analyzed </li></ul><ul><li>Protein/enzyme/RNA function can be investigated </li></ul><ul><li>Mutations can be identified, e.g. gene defects related to specific diseases </li></ul><ul><li>Organisms can be ‘engineered’ for specific purposes, e.g. insulin production. </li></ul>
  20. 20. THANK YOU
  21. 21. MODE OF REPLICATION IN PHAGE M13 VECTOR

×