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Creation of a cDNA library starts with mRNA instead of DNA. Messenger RNA carries encoded information from DNA to ribosomes for translation into protein. To create a cDNA library, these mRNA molecules are treated with the enzyme reverse transcriptase, which is used to make a DNA copy of an mRNA (i.e., cDNA). A cDNA library represents a sampling of the transcribed genes, but a genomic library includes untranscribed regions.
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A recombinant DNA molecule is produced by joining together two or more DNA segments usually originating from two different organisms. More Specifically, a recombinant DNA molecule is a vector into which desired DNA fragment has been inserted to enable its cloning in an appropriate host. Recombinant DNA molecules are produced with one of the following objectives: 1. To obtain large number of copies of specific DNA fragments. 2. Large scale production of the protein encoded by the gene. 3. Integration of the desired DNA fragment into target organism where it expresses itself. Drought tolerant-genetically modified plants: Present abiotic stress is a major challenge in our quest for sustainable food production as these may reduce the potential yields by 70% in crop plants Of all abiotic stress, drought is regarded as the most damaging Transgenic plants carrying genes for abiotic stress tolerance are being developed for water stress management Conventional breeding approaches, involving inter specific and inter generic hybridizations and mutagenesis have been limited success. Major problems have been the complexity of drought tolerance & low genetic yield components under drought conditions. Unlike conventional plant breeding there is no need of repeated back crossing Gene pyramiding or gene stacking through co-transformation of different genes with similar effects can be achieved.
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Biochem recombinant dna technology(29.6.10)
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