INTRODUCTION DEFINITION TYPES OF RECOMBINASES Cre Recombinase, Hin Recombinase, Tre recombinase, Rec A Recombinase, CONCLUSION REFERENCES

1. Types of Recombinases
By
KAUSHAL KUMAR SAHU
Assistant Professor (Ad Hoc)
Department of Biotechnology
Govt. Digvijay Autonomous P. G. College
Raj-Nandgaon ( C. G. )

2. SYNOPSIS
• INTRODUCTION
• DEFINITION
• TYPES OF RECOMBINASES
 Cre Recombinase
 Hin Recombinase
 Tre recombinase
 Rec A Recombinase
• CONCLUSION
• REFERENCES

3. INTRODUCTION
A broad category of enzymes that are involved in
the process of genetic recombination.
DNA recombinases are widely used in
multicellular organisms to manipulate the
structure of genomes, and to control gene
expression.
These enzymes, derived from bacteria and fungi,
catalyze directionally sensitive DNA exchange
reactions between short (30–40 nucleotides) target
site sequences that are specific to each
recombinase.

4. DEFINITION
 Recombinases are genetic recombination enzymes.
 An enzyme that catalyzes the exchange of short
pieces of DNA between two long DNA strands,
perticularly the exchange of homologous regions
between the paired maternal and paternal
chromosomes.
 Those circuits depend on enzymes known as
recombinases, which can cut out stretches of DNA,
flip them or insert them.

5. Types of Recombinases
These are the types of recombinases :-
 Cre Recombinase
Hin Recombinase
Tre Recombinase
Rec A Recombinase

6. Cre Recombinase
 Cre recombinase is used as a tool to modify genes
and chromosomes.
 Cre Recombinase is a tyrosine recombinase enzyme
derived from the P1 Bacteriophage.
 The enzyme requires no additional cofactors or
accessory proteins for its function.
his 34 base pair loxP recognition site consists of two
13 bp palindromic sequences.
The products of Cre-mediated recombination at loxP
sites are dependent upon the location and relative
orientation of the loxP sites.

8. HIN RECOMBINASE
 Hin recombinase is a 21
protein composed of 198
amino acids that is found in
the bacteria Salmonella.
 Hin belongs to the serine
recombinase family of
DNA.
 Hin functions by binding to
two 26bp imperfect inverted
repeat sequences.
Function of Hin
recombinase

9. Tre Recombinase
 Tre recombinase is an
experimental enzyme that in
lab tests has successfully
removed DNA inserted by
HIV from infected cells.
 The enzyme was derived
from Cre recombinase
through selective mutation
for the purposes of
identifying HIV markers.
function of Tre recombinase

10. Rec A recombinase
 RecA binds to a region of
single-stranded DNA and
catalyses the formation of
duplex DNA.
 A recombinase recognizes
and binds to each of two
recombinant sites on
different DNA molecules or
within the same DNA.
function of Rec A recombinase

11. REFERENCES
Molecular biology of the cell – Alberts
fifth Edition
 www.wikipedia.com (9:25 6/02/14)